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* Labeling density over peroxisomes: number of gold particles/0.01 jm? (+ SEM) of 15-nm or 5-nm diameter was compared between the electron-lucent area and the electron-dense area in the matrix. Fifteen-nm and 5-nm particles indi- cate the localization of D-amino acid oxidase and catalase, respectively. The labeling density for each enzyme was significantly different between the two areas (p<0.01). Statistical analysis was done by Student's ¢-test. Table 1. Labeling indices of 15-nm and 5-nm colloidal gold particles showing the localization of D-amino acid oxidase and catalase over electron-lucent and electron-dense areas in peroxisomal matrix?

Table 1 * Labeling density over peroxisomes: number of gold particles/0.01 jm? (+ SEM) of 15-nm or 5-nm diameter was compared between the electron-lucent area and the electron-dense area in the matrix. Fifteen-nm and 5-nm particles indi- cate the localization of D-amino acid oxidase and catalase, respectively. The labeling density for each enzyme was significantly different between the two areas (p<0.01). Statistical analysis was done by Student's ¢-test. Table 1. Labeling indices of 15-nm and 5-nm colloidal gold particles showing the localization of D-amino acid oxidase and catalase over electron-lucent and electron-dense areas in peroxisomal matrix?

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Figure 1. Electron micrograph of rat hepatocyte stained for D-amino acid oxidase by the protein A-gold technique. Gold particles for 0-amino acid oxidase were localized over the peroxisomal matrix. Peroxisomes are shown by arrowheads. Original magnification x 35,000. Bar = 0.5 um.
Figure 1. Electron micrograph of rat hepatocyte stained for D-amino acid oxidase by the protein A-gold technique. Gold particles for 0-amino acid oxidase were localized over the peroxisomal matrix. Peroxisomes are shown by arrowheads. Original magnification x 35,000. Bar = 0.5 um.
Figure 2. Electron micrograph of peroxisome of a rat hepatocyte stained for D-amino acid oxidase by the protein A-gold technique. Note the localization of gol particles over an electron-lucent area in the peroxisome. Original magnification x 100,000. Bar = 0.5 um. Figure 3. Electron micrograph of peroxisome of a rat hepatocyte stained for D-amino acid oxidase by the protein A-gold technique on rapid-frozen and freeze- substituted tissue processed without any chemical fixatives. Note the localization of gold particles over a small area in the peroxisome. Original magnification x 90,000. Bar = 05 um.
Figure 2. Electron micrograph of peroxisome of a rat hepatocyte stained for D-amino acid oxidase by the protein A-gold technique. Note the localization of gol particles over an electron-lucent area in the peroxisome. Original magnification x 100,000. Bar = 0.5 um. Figure 3. Electron micrograph of peroxisome of a rat hepatocyte stained for D-amino acid oxidase by the protein A-gold technique on rapid-frozen and freeze- substituted tissue processed without any chemical fixatives. Note the localization of gold particles over a small area in the peroxisome. Original magnification x 90,000. Bar = 05 um.
Figures 6-11. Electron micrographs of peroxisomes stained for matrix enzymes by the protein A-gold technique. Figure 6, catalase; Figure 7, acyl-CoA oxidase; Figure 8, enoy!-CoA hydratase; Figure 9, 3-ketoacyl-CoA thiolase; Figure 10, L-a-hydroxy acid oxidase; and Figure 11, glycolate oxidase. Note the presence of an electron-lucent area in the matrix negative for labeling by the antibody for each enzyme (arrows). Original magnification x 80,000. Bar = 0.5 um.
Figures 6-11. Electron micrographs of peroxisomes stained for matrix enzymes by the protein A-gold technique. Figure 6, catalase; Figure 7, acyl-CoA oxidase; Figure 8, enoy!-CoA hydratase; Figure 9, 3-ketoacyl-CoA thiolase; Figure 10, L-a-hydroxy acid oxidase; and Figure 11, glycolate oxidase. Note the presence of an electron-lucent area in the matrix negative for labeling by the antibody for each enzyme (arrows). Original magnification x 80,000. Bar = 0.5 um.
Figure 12. Electron micrograph of peroxisome doubly stained for 0-amino acid oxidase (15-nm gold particles) and catalase (5-nm gold particles). Some of the 5-nm gold particles are shown by arrowheads. Original magnification x 200,000. Bar = 0.5 um.
Figure 12. Electron micrograph of peroxisome doubly stained for 0-amino acid oxidase (15-nm gold particles) and catalase (5-nm gold particles). Some of the 5-nm gold particles are shown by arrowheads. Original magnification x 200,000. Bar = 0.5 um.
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