Table 4 Genes’ interaction values for yeast25-alpha. In this section we deliver an example of KSG application to Genetic Regulatory System (GRS) that is a mechanism for determining when and in what form a gene or genes will be expressed [15]. Shortly, KGS is utilized to produce knowledge regarding the genes behavior in GRS for estimating their interaction’ s behavior and what the most expressive gene during a sequence of interaction times. By having much knowledge regarding those phenomena, we can manage the GRS to produce better results. We use the simplest gene database namely yeast25- alpha database which consists of 25 genes with 18t interaction times as presented in Table 4. Figure 6 depicts the knowledge gains by KGS from time-to-time with single gene, 12 genes, and all the 25 genes. The last figure shows the original data.