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Fic. 4.—Expansion of probe p; with neighboring probes Pi-2, Pi-1, Pis1, and Piso. Probes are diagrammed in an intact DNA region with restriction cleavage sites indicated and also in homologous cloned DNA segments, C,, Cz, C3, and C4, which may contain parts of some probes.

Figure 4 Fic. 4.—Expansion of probe p; with neighboring probes Pi-2, Pi-1, Pis1, and Piso. Probes are diagrammed in an intact DNA region with restriction cleavage sites indicated and also in homologous cloned DNA segments, C,, Cz, C3, and C4, which may contain parts of some probes.

Related Figures (5)

Fic. 1.—a, Cuts made in pair of homologous chromosomes by enzyme A and enzyme B; b, hybridization pattern of enzymes A and B given cuts of a.
Fic. 1.—a, Cuts made in pair of homologous chromosomes by enzyme A and enzyme B; b, hybridization pattern of enzymes A and B given cuts of a.
Fic. 2. —Expected LOD score as a function of recombination fraction for two 4-member pedigrees To answer this question, we must know how large the human genome is in genetic terms and how far one can be from a marker and still detect linkage. Renwick [4] has estimated that the human genome is 33 Morgans in length. Figure 2, from [22], shows that the expected LOD score for two linked markers in small family structures falls by an order of magnitude between @ = .1 and 6 = .3, where @ is the recombination fraction. Therefore, a search for linkage will be very inefficient at greater values of 0. For this discussion, an equivalence between Morgans and recombination fraction is assumed, which is approximately true within this range of values. We will pick 6 = .2 as the maximum distance desired between RFLP marker loci which will still allow linkage to be detected. To map a new RFLP, a dominant gene for a disease, or a gene for a carrier trait, RFLPs spaced 0.2 Morgans apart will be sufficient to guarantee that a gene is detectably linked to an RFLP locus at a distance no greater than 6 = .1. At this distance, and without further dividing the 33 Morgans into individual chromosome
Fic. 2. —Expected LOD score as a function of recombination fraction for two 4-member pedigrees To answer this question, we must know how large the human genome is in genetic terms and how far one can be from a marker and still detect linkage. Renwick [4] has estimated that the human genome is 33 Morgans in length. Figure 2, from [22], shows that the expected LOD score for two linked markers in small family structures falls by an order of magnitude between @ = .1 and 6 = .3, where @ is the recombination fraction. Therefore, a search for linkage will be very inefficient at greater values of 0. For this discussion, an equivalence between Morgans and recombination fraction is assumed, which is approximately true within this range of values. We will pick 6 = .2 as the maximum distance desired between RFLP marker loci which will still allow linkage to be detected. To map a new RFLP, a dominant gene for a disease, or a gene for a carrier trait, RFLPs spaced 0.2 Morgans apart will be sufficient to guarantee that a gene is detectably linked to an RFLP locus at a distance no greater than 6 = .1. At this distance, and without further dividing the 33 Morgans into individual chromosome
MATING TYPES, PROBABILITY THAT OFFSPRING ARE INFORMATIVE, AND FREQUENCY OF EACH TYPE FOR THE LINKAGE MODELS TABLE 1
MATING TYPES, PROBABILITY THAT OFFSPRING ARE INFORMATIVE, AND FREQUENCY OF EACH TYPE FOR THE LINKAGE MODELS TABLE 1
* Data compiled from: Mourant AC, Kopec AC, Domaniewska-Sobczak: The Distribution of the Human Blood Groups and Other Polymorphisms , 2nd ed. London, Oxford Univ. Press, 1976 PIC oF 29 CODOMINANT HUMAN CHROMOSOME MARKER LOCI IN CAUCASIANS
* Data compiled from: Mourant AC, Kopec AC, Domaniewska-Sobczak: The Distribution of the Human Blood Groups and Other Polymorphisms , 2nd ed. London, Oxford Univ. Press, 1976 PIC oF 29 CODOMINANT HUMAN CHROMOSOME MARKER LOCI IN CAUCASIANS
Fic. 3. —Expected LOD score for two recombination fractions (r) as a function of sibship size
Fic. 3. —Expected LOD score for two recombination fractions (r) as a function of sibship size
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