Bacteriological Spectrum of Neonatal Sepsis

International Journal of Contemporary Pediatrics

Background: The present study was designed to evaluate the clinical spectrum, bacteriological profile, antibiotic sensitivity pattern and mortality due to neonatal septicemia in neonates admitted in neonatal units attached to the SMS Medical College, Jaipur.Methods: In born and out born babies of postnatal age up to 28 days who were bacteriological proven cases of septicemia were subjected to history, clinical examination and laboratory evaluation and data were analyzed statistically.Results: Out of 150 cases 67.33% neonates were preterm and 77.33% were low birth weight neonates. Gram negative organisms were most common cause of septicemia (66.6%), Both the Gram negative and Gram positive organisms were sensitive to piperacillin-tazobactam, linezolid, fosfomycin, teicoplanin, polymyxin B, colistin, ofloxacin. Both the Gram negative and Gram positive organisms were resistant against cephalosporins, ampicillin, Amoxyclav, cotrimoxazole. Gram negative isolates were most sensitive to Po...

Journal of Pharmaceutical Research International

Background: The drug resistance and pathogens are different in various Hospitals of any country. Very high resistance pattern is observed nowadays to the frequently used antibiotics. The important observation has been noted that most of the doctors do not obtain blood cultures before start of the antibiotics, which becomes competent source of resistance. For the same purpose, this study has been done to find out the responsible microbes causing ailment and their susceptibility towards antibiotics to plan early and effective management. Materials and Methods: A number of 100 new borns admitted in the NICU CMC Children Hospital Larkana with signs and symptoms of sepsis were included in this research work. The study will help in provision of a comprehensive record on microorganisms causing sepsis in the neonates and their antibiotic sensitivity. The epidemiology and presence of neonatal sepsis in particular area makes it more easy and convenient to implement the rationale of empirical ...

Journal of clinical and diagnostic research : JCDR, 2013

Neonatal sepsis is one of the most common causes of neonatal mortality and morbidity, particularly in the developing countries. Its causative bacteria and their respective sensitivity patterns are different in each hospital and region. The objective of this study was to determine the causative bacteria and pattern of susceptibility to antibiotics in NICU of a tertiary care centre, which in turn may help in implementation of empirical therapy. This prospective study was carried out at a medical college during the period from 1st April 2011 to 31st March 2013. A total of 364 cases of suspected sepsis were admitted in our NICU during the mentioned period. Out of which, 137 cases were positive for culture. All the neonates of suspected sepsis were screened by using a panel consisting of CRP, ANC, I/T ratio, micro ESR and culture and sensitivity. A total of 137 cultures were found to be positive out of 364 cases. The most common organism isolated was Staphylococcus aureus (37.22%) follow...

THE PROFESSIONAL MEDICAL JOURNAL, 2017

ORIGINAL PROF-3914 ABSTRACT… Introduction: Neonatal sepsis is a systemic condition characterized by bacteremia that occurs in the first month of life. It is a fatal condition and need to be treated promptly. Bacterial isolates include both gram positive and negative bacteria and the cure of condition is highly dependent on antimicrobial drug sensitivity and resistant patterns. It is there for utmost important to known commonly occurring bacteria in neonatal septic states and their drug sensitivity patterns. Objectives: To determine the frequency of the bacterial isolates in blood and their sensitivity patterns to commonly used antibiotics in neonatal sepsis. Setting: Neonatal intensive care unit(NICU), Department of Shifa International Hospital. (SIH), Islamabad. Study Design: Cross sectional. Duration: This study was conducted between 6 1 st June 2013 to 30 th November 2013. Subject and Methods: A total of 180 neonates, admitted in NICU with evidence of clinical sepsis i.e. with signs and symptoms suggestive of septicemia (fever, lethargy, reluctance to feed, seizures, and irritability) were included in this study. The samples for blood cultures were taken. Identification of bacterial isolates was carried out by the standard bacteriological techniques, which include gram staining and bacterial cultures and antimicrobial sensitivity patterns which was performed by modified Kirby and Bauer disc diffuse method as per CLSI (Clinical and Laboratory StandardsInstitute)guidelines.A predesigned Performa was filled. Results: Culture revealed bacterial growth in 7.2% samples. Gram negative organisms were observed in 6.67% and only 1 were gram positive. In this study, 50% and 100% of E-coli were sensitive to ampicillin, meropenem and amikacin, gentamycin respectively. Sixty to 100% of pseudomonas was sensitive to ceftazidime, tazobactum, meropenem and 100% of enterococcus was sensitive to ampicillin and vancomycin. Conclusion: Antimicrobial drug resistance and constantly changing resistance patterns is emerging issues in various groups of infections and septic states, especially for routinely used antibiotics as found in our study. Thus by prescribing rational use of antimicrobial as per bactriogram, It'll be easier totreat sepsis effectively and economically and reduce the mortality and morbidity related to neonatal sepsis.

Pakistan Journal of Health Sciences

Sepsis in newborns is a serious medical condition having a high mortality. Pakistan and other developing countries have a high burden of neonatal sepsis. Objectives: To determine the bacterial spectrum and antibiotic activity pattern in neonatal sepsis. Methods: This retrospective cross-sectional research was performed at the Pediatrics Unit of Ziauddin University Hospital Karachi from 1st June 2022 till 1st December 2022. A total of 120 medical records of neonates admitted with the clinical suspicion of sepsis were reviewed. Non-probability convenience sampling technique was used. Data regarding clinical characteristics of neonates, type of bacteria isolated and antibiotic susceptibility results were recorded. SPSS version 20 was used for statistical analysis. The results were written as frequencies / percentages. Results: Neonatal sepsis was suspected in 120 newborns but blood culture-proven infection was present in 32 (13.3%) neonates with 23(71.8%) having early -onset sepsis. T...

2015

Objectives: Neonatal sepsis is one of the major causes of morbidity and mortality in the newborn. Early diagnosis and appropriate treatment of blood stream infections would minimise the risk besides reducing emergence of multidrug resistant organisms. Therefore the present study was done to know the etiological agents of neonatal sepsis, and their antimicrobial susceptibility pattern. Methods: All neonates with signs and symptoms of neonatal septicaemia were enrolled in the study. Blood culture was done by conventional method. Any growth was identified by colony characteristics and standard biochemical tests. Antimicrobial susceptibility tests was done by Kirby Bauer Disc Diffusion method according to National Committee for Clinical Laboratory Standards (NCCLS) guidelines. Results: 115 cases were enrolled in the study . Out of them early onset sepsis occurred in 76(66.08%)and late onset sepsis in 39 (39%) neonates. Rates of infection was high in males (60%) as compared to females(40...

The Professional Medical Journal, 2020

Objectives: We planned this study to observe the pattern related to bacterial isolates causing neonatal septicemia along with their pattern of antibiotic susceptibility. Study Design: Prospective study. Setting: Department of Neonatology, Sheikh Khalifa Bin Zaid Al Nahyan Teaching Hospital, Rawlakot. Period: From 1st January 2018 to 30th June 2018. Material & Methods: Prior to empiric antibiotic usage, blood was collected from neonates having clinical sepsis and sent for blood culture. A total of 60 neonates with culture proven sepsis were enrolled for this study. Reports of blood culture were evaluated for isolates of bacteria as well as patterns of sensitivity for frequently used antibiotics in the institution. SPSS version 21.0 was used for data entry and analysis. Results: A total of 60 neonates having culture proven sepsis were enrolled in the current study. Amongst these, 39 (65.0) were male and 21 (35.0%) female. Early onset of sepsis was diagnosed in most, 42 (70.0%) neonate...

International Journal of Pediatrics

Background. Neonatal sepsis remains one of the leading causes of mortality and morbidity in developing countries. With a dearth of data on neonatal sepsis in our country, this study was conducted to determine the incidence of clinical neonatal sepsis and evaluate the clinical, bacteriological, and antimicrobial susceptibility profile of organisms. Material and Methods. A prospective cross-sectional study was conducted in the Neonatal Unit of the National Hospital from 1st January to 31st December 2016. All neonates admitted with suspected clinical sepsis were included. Sepsis screens and cultures were sent under aseptic conditions. Data was analyzed using STATA™ version 12. Clinical features and neonatal and maternal risk factors were analyzed using chi-squared test. Bacteriological profile was analyzed with descriptive statistics. Results. During the study period, incidence of culture positive neonatal sepsis was 19 per 1000 admissions with a blood culture positivity rate of 14%. 5...

African journal of microbiology research

The spectrum of bacteria which cause neonatal sepsis varies in different parts of the world. Antibiotic resistance is an increasing problem of these bacteria. The aim of this study was to detect the most common bacterial causes of neonatal sepsis and determination of their sensitivity to antibiotics. In a descriptive cross-sectional study, records of the neonates suspicious to neonatal sepsis who were admitted to Neonatal Intensive Care Unit (NICU) were assessed. Neonates with positive blood culture along with clinical signs of sepsis were entered in the study. Evaluated data included: age, sex, birth weight, type of infection, type of isolated pathogen, antibiotic sensitivity and disease outcome. Of 3700 screened blood cultures, 153 (4.1%) reported as positive, 76.5% of the evaluated neonates were preterm, 142 (92.8%) had gram negative sepsis and 11 (7.2%) had gram positive sepsis. The most common isolated gram negative bacteria were Klebsiella pneumoniae (46.4%), enterobacter spp. (17.6%) and Escherishia coli (14.4%). Coagulase negative staphylococci (CONS) were the most prevalent gram positive bacteria (5.9%) in this study. All of the klebsiella and enterobacter strains were resistant to ampicillin and gentamicin. The sensitivity of K. pneumoniae and enterobacter to imipenem was: 90 and 92.5%, respectively. Prevalence of early and late-onset sepsis was 64.7 and 35.3% respectively. Mortality rate of sepsis in this study was reported as 53.5%. In conclusion, the most common cause of bacterial sepsis in this study was K. pneumoniae which was completely resistant to ampicillin and gentamicin. So empirical treatment of sepsis with ampicillin and gentamicin in Imam Khomeini NICU must be reconsidered.

Journal of Rawalpindi Medical College, 2021

Objective: To find out the most common organisms involved in neonatal sepsis origination and observe the pattern of antibiotic sensitivity and resistance of bacterial isolates. Materials and Methods: This descriptive cross-sectional study was conducted at the Department of Paediatrics Izzat Ali Shah Hospital, Wah Cantt. Out of 420 patients admitted with sepsis in NICU, 19.5% of patients with positive blood cultures were included in the study. A consecutive, non-probability sampling technique was used. Results: Out of 82 positive blood cultures gram-positive bacteria were observed in 19 patients (23.2%) and gram-negative bacteria were seen in 63 patients (76.8%). The most common gram-negative pathogens isolated were Acinetobacter (29.3%) and Klebsiella (24.4%). Staphylococcus aureus (12.2%) was the commonest gram-positive organism isolated. Gram-negative organisms showed maximum sensitivity to Tigecycline and Colistin and were resistant to Cefixime, Aztreonam, Amoxicillin, and Ceftri...