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On-Chip Spyhole Mass Spectrometry for Droplet-Based Microfluidics

Profile image of Hubert GiraultHubert Girault

2014, Angewandte Chemie International Edition

https://doi.org/10.1002/ANIE.201310795
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6 pages

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Abstract

For efficient coupling of droplet-based microfluidics with mass spectrometry (MS), a spyhole drilled on the top of a microchip is used to sample the passing droplets by electrostatic-spray ionization (ESTASI) MS. The technique involves placing an electrode below the chip under the spyhole and applying high-voltage pulses. Electrospray occurs directly from the spyhole, and the droplet content is analyzed by MS without a dilution or oil removal step. To demonstrate the versatility of this technique, we have successfully monitored a droplet-based tryptic digestion, as well as a biphasic reaction between b-lactoglobulin in water and a-tocopheryl acetate in 1,2-dichloroethane, where the protein extracts the antioxidant from the oil phase and becomes reduced.

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