Potential value of slug secretions in the treatment of wounds

2021

Collagen production and deposition is crucial to replace damaged tissue. Glycosaminoglycans in snail mucus has high biological value for wound healing. This study aims to determine the effect of concentration differences of snail mucus gel on collagen density and wound closure rate in skin punch biopsy wounds. Quasi experimental research was done using 6 Wistar rats, each wounded with 4 punch biopsy wound in dorsa skin, and subsequently applied with 24%, 48%, 96% snail mucus gel and 3% CMC-Na gel. Collagen density was measured on 2nd and 7th day using histophotometry analysis. Wound closure rate was measured from wound area on the 4th and 7th day.Data analysis using 2-way Anova showed significant differences in collagen density (p=0.000) and wound closure rate (p=0.000) of all treatment groups and observation period, but there is no interaction between them. Post hoc LSD showed significant difference in wound closure rate between control groups and all treatment groups (p=0.000), bu...

Saudi Journal of Medicine, 2020

Background: Wound healing is the process of repair that follows injury to the skin and other soft tissues. It is a natural restorative response to tissues injury. In the primitive years a lot of substances were considered to be medicinal and were used to enhance wound size contraction and closure. Similarly, the Iwere people of Delta State, Nigeria are known for using Achantina fulica species shell for wound healing although there is no known empirical study showing its ability to enhance wound contraction. Thus, this study was aimed at assessing the wound healing effect of powdered snail shell of Achantina fulica species on the wound sizes of rat. Materials and Methods: Eight wistar rats were used and grouped into experimental and control groups. Paravertebral round wound of 2cm were made on the skin of each rat under light chloroform anesthesia. The wounds were treated with powdered snail shell and 0.9% normal saline for the experimental and control group respectively. Wound sizes were measured everyday using a meter rule before redressing. Results and Discussions: Day 1, 2, 3, showed percentage reduction of 100%, 99% 92. 5% for those of the experimental group while 99%, 94% and 90% for those of the control group. On day 4, the percentage reduction was 72% for the experimental while 86% for the control group. This was the first outstanding wound size reduction observed in rats dressed with powdered snail shell. The wound size reduction continued from day 5-12 with a percentage reduction of 61.5%, 50%, 40%, 34%, 26.5%, 24%, 15%, 9% for the experimental group while that of the control group was 86%, 76.5%, 65%, 63.7% 57.5%, 55%, 5 1.5%, 47.5%, 42.5%. Conclusion: The wounds treated with powered snail shell by day 13 was completely healed while those on 0.9% normal saline took 21 days to heal. It suggests that the powdered Achantina fulica shell promoted the wound size contraction which further led to a rapid healing and wound closure earlier than the control in the study. It could be inferred that this Achantina fulica shell powder could be refined and used as an alternative substance for wound healing.

Biotechnology Progress, 2006

The resurgence of larval biotherapy as a debridement tool in wound management has been accompanied by several clinical reports highlighting concomitant tissue regeneration. Studies employing in vitro cell motility assays have found that purified excretory/secretory (ES) products from Greenbottle larvae (blowfly, Lucilia sericata) are motogenic for human dermal fibroblasts when used as a supplement in culture media. The objective of the present study was to determine whether ES delivered using a prototype hydrogel wound dressing induced similar motogenic effects on fibroblastic (3T3) and epithelial cells (HaCaTs) comprising a scratched-monolayer wound model. Quantitative analysis by MTT assay failed to detect significant mitogenic effects of ES on either cell type. Quantitative image analysis revealed that ES exposure markedly accelerated wound closure through a motogenic effect on both fibroblasts and keratinocytes. Quantitative histochemical analysis detected significantly higher phosphotyrosine (pTyr) expression in ES-exposed cell cultures than in controls; moreover immunocytochemistry revealed conspicuously raised levels of pTyr expression in cells located at the wound margin. By attenuation with a panel of enzyme inhibitors these effects were attributed to the protease components of ES. The present results suggest that controlled delivery of ES as a follow-up to maggot debridement therapy may be an effective therapeutic option for stimulation of tissue regeneration in wound management.

Acta cirurgica brasileira, 2018

To analyzed the healing effect of the powdered shell of the Megalobulimus lopesi snail on wounds of diabetic rats, since in non-diabetic rats the powdered shell presented healing potential. Seventy-two Wistar rats (Rattus norvegicus albinus) were divided into three groups: Control group (GC.diab), no therapeutic intervention on the wound; Vehicle's Control group, topical via, in diabetic rats (GCvt.diab): Powder Shell Group (PC) applied topically (GPCvt.diab): Experimental group was administered topically shortly after wound dressing and once a day during the experimental period (3, 7, 14 and 21 days) the composition containing the powdered shell of the snail. The following variables related to the healing potential were analyzed: macroscopic one, where the capacity of reduction of the wound area was evaluated; histological analysis in HE, angiogenic activity, morphometric analysis (re-epithelization), leukocyte inflammatory infiltrate; leukocyte count and also differentiation i...

International Journal of Clinical Practice, 2007

Acta Poloniae Pharmaceutica - Drug Research, 2022

Natural origin substances with healing properties of potential use in the treatment of different diseases, particularly wound management, remain in the scientific and research interest for many years. The substance of natural origin with potentially wide use in medicine and pharmacy is snail mucus. The study aims to evaluate the effect of snail mucus extract on human fibroblasts and evaluate the biological and cytotoxic activity of this substance. In the present study, human NHDF fibroblast cells were exposed to snail slime at two different concentrations: 15 µL/mL and 70 µL/mL. Moreover, in this study, the assessment of the biological activity of cells was conducted using mitochondrial redox activity. At the same time, the analysis of the mucus sample was carried out throughout the infrared spectroscopy implementation. It has been shown that this substance contains compounds such as mucin, achacin, mitomycin, allantoin. The snail mucus affects the viability of fibroblasts-connective tissue cells found in the dermis, responsible for collagen and elastin production. The mechanism of positive action on human fibroblasts is not entirely known and requires further research.

F1000Research, 2021

Background: Angiogenesis is the process through which new blood vessels are formed from existing ones. This process plays an important role in supplying the oxygen and nutrients needed for cellular metabolism and eliminating cell debris during wound healing. Snail mucus can bind to several factors that stimulate angiogenesis, including vascular endothelial growth factor, platelet-derived growth factor, and fibroblast growth factor. The aim of this study is to observe changes in angiogenesis during the healing of wounds topically applied with snail mucus. Methods: Punch biopsy was performed on the back of male Wistar rats to obtain four wounds, and different concentrations of snail mucus were applied to each of these wounds. The animals were sacrificed on days 2, 4, and 7 to observe the extent of angiogenesis during wound healing by microscopy. Results: Two-way ANOVA showed differences in number of blood vessels formed (p = 0.00) and day of observation (p = 0.00) between groups. Post...

Journal of Applied Science, Engineering, Technology, and Education, 2023

Snail mucus Achasin protein is a protein that has important biological functions, besides being intended to prevent evaporation, and assist smooth movement, it is also needed to protect the body from mechanical injuries. This research is expected to increase knowledge about the effect of achasin protein on snail mucus on wound healing and can provide scientific information to the public about the use and benefits of snail mucus as a traditional medicine for healing cuts on the skin of mice (Mus Musculus). This type of research is a literature review study that aims to examine the effect of achasin protein on snail mucus (Achatina Fulica) on the healing of cuts on the skin of mice (mus musculus). Sources of data used in this study used a literature review with relevant sources such as books, official documents, publications, and relevant research results. The results of this study were the achasin protein in snail mucus (Achatina Fulica) can accelerate the healing of cuts (Vulnus Scissum) on the skin of mice (mus musculus). The content of achasin isolate is also useful as an antibacterial and painkiller. So that people can take advantage of snail slime as an alternative to traditional medicine derived from natural ingredients that have been clinically tested for their efficacy.

Biomedicine, 2023

Chemical composition, mineral profile, anti-bacterial, and wound Chemical composition, mineral profile, anti-bacterial, and wound healing properties of snail slime of Helix aspersa Müller healing properties of snail slime of Helix aspersa Müller

Ulcers, 2013

Chronic wounds are a signi�cant health problem in the United States, with annual associated costs exceeding $20 billion annually. Traditional wound care consists of surgical debridement, manual irrigation, moisture retentive dressings, and topical and/or systemic antimicrobial therapy. However, despite progress in the science of wound healing, the prevalence and incidence of chronic wounds and their complications are escalating. e presence � complexity of bacterial bio�lms in chronic wounds has recently been recognized as a key aspect of non-healing wounds. �acterial bio�lms are sessile colonies of polymicrobial organisms (bacteria, fungus, etc.) enclosed within a self-produced exopolymeric matrix that provides high levels of tolerance to host defenses, antibiotics and antiseptics. us, there is a need for alternative therapies to reduce bio�lms in chronic wounds. In this report, we present initial �ndings from in vitro experiments which show that larval debridement therapy with disinfected blow �y larvae (Phaenicia sericata) reduced total CFUs (�-logs) of planktonic and mature bio�lms of Pseudomonas aeruginosa or Staphylococcus aureus grown on dermal pig skin explants by 5-logs a�er 24 hours of exposure, and eliminated bio�lms (no measurable CFUs) a�er 48 hours of exposure.