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Biology

Why is chlorophyll b only used in light-harvesting systems?

Profile image of Kenlo NasaharaKenlo Nasahara

2018, Journal of Plant Research

https://doi.org/10.1007/S10265-018-1052-7
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Abstract

Chlorophylls (Chl) are important pigments in plants that are used to absorb photons and release electrons. There are several types of Chls but terrestrial plants only possess two of these: Chls a and b. The two pigments form light-harvesting Chl a/b-binding protein complexes (LHC), which absorb most of the light. The peak wavelengths of the absorption spectra of Chls a and b differ by c. 20 nm, and the ratio between them (the a/b ratio) is an important determinant of the light absorption efficiency of photosynthesis (i.e., the antenna size). Here, we investigated why Chl b is used in LHCs rather than other light-absorbing pigments that can be used for photosynthesis by considering the solar radiation spectrum under field conditions. We found that direct and diffuse solar radiation (PARdir and PARdiff, respectively) have different spectral distributions, showing maximum spectral photon flux densities (SPFD) at c. 680 and 460 nm, respectively, during the daytime. The spectral absorban...

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Chlorophyll (Chl) molecules attached to plant lightharvesting complexes (LHC) differ in their spectral behavior. While most Chl a and Chl b molecules give rise to absorption bands between 645 nm and 670 nm, some special Chls absorb at wavelengths longer than 700 nm. Among the Chl a/b-antennae of higher plants these are found exclusively in LHC I. In order to assign this special spectral property to one chlorophyll species we reconstituted LHC of both photosystem I (Lhca4) and photosystem II (Lhcb1) with carotenoids and only Chl a or Chl b and analyzed the effect on pigment binding, absorption and fluorescence properties. In both LHCs the Chl-binding sites of the omitted Chl species were occupied by the other species resulting in a constant total number of Chls in these complexes. 77-K spectroscopic measurements demonstrated that omission of Chl b in refolded Lhca4 resulted in a loss of long-wavelength absorption and 730-nm fluorescence emission. In Lhcb1 with only Chl b long-wavelength emission was preserved. These results clearly demonstrate the involvement of Chl b in establishing longwavelength properties.

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    Beenish Afzal

    Frontiers in Plant Science, 2021

    Water-scarce areas are continually increasing worldwide. This factor reduces the quantity and quality of crops produced in affected areas. Physical seed treatments are considered economical and ecofriendly solutions for such problems. It was hypothesized that a moderately drought-tolerant crop grown from seeds treated with a He-Ne laser utilizes water-limited conditions better than plants grown from untreated seeds. A field study was conducted, growing a moderately drought tolerant crop (sunflower) with supportive seed treatment (He-Ne laser treatment at 300 mJ) for 0, 1, 2, and 3 min. Thirty-day-old plants were subjected to two irrigation conditions: 100% (normal) and 50% (water stress). Harvesting was done at flowering (60-day-old plants) at full maturity. The sunflowers maintained growth and yield under water limitation with a reduced achene number. At 50%, irrigation, there was a reduction in chlorophyll a, a+b and a/b; catalase activity; soluble sugars; and anthocyanin, alongsi...

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    2019 Awards in the Journal of Plant Research
    Kouki Hikosaka

    Journal of Plant Research, 2019

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