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Intercellular adhesive specificity

Profile image of Stephen RothStephen Roth

1976, Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes

https://doi.org/10.1016/0304-4157(76)90005-8
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Intercellular adhesive specificity plays a crucial role in the precision of cellular interactions essential for development and physiology. However, direct experimental evidence and understanding of the molecular mechanisms behind cellular recognition remain limited. Recent advancements in measurement techniques have opened avenues for studying biological processes related to intercellular adhesion, emphasizing its significance in morphogenetic events such as embryogenesis and gastrulation in various model organisms. The review discusses these biological processes alongside the methods developed for analyzing adhesive specifics and suggests future directions for exploring the complexities of intercellular interactions.

Figures (3)
Fig. 1. Dictyostelium discoideum cells in end to end contact during aggregation. Each cell is about 25 um in length. Different areas of the surface of a single cell appear to possess different adhesive properties. (Scanning electron micrograph from Chang et al. [198]).
Fig. 1. Dictyostelium discoideum cells in end to end contact during aggregation. Each cell is about 25 um in length. Different areas of the surface of a single cell appear to possess different adhesive properties. (Scanning electron micrograph from Chang et al. [198]).
Fig. 2. Protocols for collection assays. A single-cell suspension, prepared from one tissue and labeled with a radioactive isotope, is exposed to collecting surfaces of similar shape and area prepared from any number of tissues. Since the collision frequencies between the surfaces and the labeled cells are identical, the number of radioactive cells adhering to a surface gives a direct estimate of the relative adhesivity between the cells in suspension and the cells making up that surface.
Fig. 2. Protocols for collection assays. A single-cell suspension, prepared from one tissue and labeled with a radioactive isotope, is exposed to collecting surfaces of similar shape and area prepared from any number of tissues. Since the collision frequencies between the surfaces and the labeled cells are identical, the number of radioactive cells adhering to a surface gives a direct estimate of the relative adhesivity between the cells in suspension and the cells making up that surface.
Fig. 3. Scanning electron micrograph of a 3T12 fibroblast adhering to monolayer of 3T12 cells. In this experiment, the cell suspension was preincubated with colloidal gold. The inset, obtained using high energy electrons, shows a bright reflection from the gold and identifies the cell as originating from the cell suspension. (From Howard et al. [199]).
Fig. 3. Scanning electron micrograph of a 3T12 fibroblast adhering to monolayer of 3T12 cells. In this experiment, the cell suspension was preincubated with colloidal gold. The inset, obtained using high energy electrons, shows a bright reflection from the gold and identifies the cell as originating from the cell suspension. (From Howard et al. [199]).

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