Papers by Luma Abdul-Qadir
Effect of Sorbitol on Callus Induction and Somatic Embryos Regeneration in Two Local Wheat (Triticum aestivum L.) Cultivars
Journal of Basrah Researches (Sciences), 2013
A series of experiments were conducted in Plant Two local wheat varieties cultivars named Dor29 a... more A series of experiments were conducted in Plant Two local wheat varieties cultivars named Dor29 and Fateh were evaluated to determine the impact of 0, 10, 20 and 30 g.lP-1 P sorbitol for callus induction and somatic embryos regeneration. Mature seeds of the tested cultivars were selected as explants for callus induction and regeneration on MS medium with Gambrog vitamins containing 30 g.lP-1 P sucrose, 5 g.lP-1 P agar, 2 mg.lP-1 P BA and 1 mg.lP-1 P 2,4-D. Results were showed that sorbitol at 20 g.lP-1 P had a significant effect on callus fresh weight; mean globular somatic embryos number with no significant difference between the two cultivars under investigation. The obtained results from our study suggested that the optimal callus induction medium was MS + Gambrog vitamins + 30 g.lP-1 P sucrose + 20 g.lP-1 P sorbitol + 2 mg.lP-1 P BA + 1 mgP-P 2,4-D + 5 g.lP-1 P agar ,while maximum number of somatic embryos formed at the same previous medium with the amition of BA and 2,4-D.
Journal of Biology, Agriculture and Healthcare, 2017
Laboratory experiment using hydroponic culture system was conducted at plant physiology laboratory,
Journal of Biology, Agriculture and Healthcare, 2016
The study was conducted to determine the appropriate concentration of coconut water (CW) that enh... more The study was conducted to determine the appropriate concentration of coconut water (CW) that enhance an efficient and cost effective protocol for rice micropropagation through callus induction and somatic embryogenesis.Seeds of a local Iraqi rice (Oryza sativa L.) cultivar Amber33, were used as explant which inoculated on basal medium consists of MS inorganic salts, Gamborg vitamins and supplemented with the auxin 2,4-Dichlorophenoxyacetic acid (2,4-D) and the cytokinin Benzyl adenine (BA). In order to evaluate the role of CW, three concentrations (10, 20 and 30 g.l-1) were added to MS inorganic salts after the exclusion of growth regulators (2.4-D and BA) and Gamborg vitamins. Results showed that adding CW especially at concentration of 30g.l-1 have minimized the callus induction period, enhanced callus fresh weight and numbers and germination percentage of somatic embryos when compared with the media enriched with growth regulators only.
Muthanna journal of agricultural science, Aug 4, 2021
Effect of EDTA chelating on bioccmulation of lead ion in alfalfa plant (medicago astiva L.
Effect of water stress on callus induction from shoot tips of date palm (Phoenix dactylifera L.) cv. Bream cultured in vitro
Basrah Journal For Date Palm Research, Dec 28, 2011
The results indicate that using PEG 3000 with previous concentrations caused significant increase... more The results indicate that using PEG 3000 with previous concentrations caused significant increase in callus fresh weight when compared with nutrient medium free of PEG 3000 with no significant difference between the two concentrations; while there was a significant increase in somatic embryos produced by nutrient medium with 10% PEG 3000 compared with that having 20% PEG or control treatment free of PEG.
The effect of water stress on callus and somatic embryos formation of rice (Oryza sativa L.) cv. Jasmine cultured in vitro
Journal of Basrah …, 2011
... Abdul-Qadir & Al-Ka'aby References 1-AM Al-Bahrany. Pak.J.Bi... more ... Abdul-Qadir & Al-Ka'aby References 1-AM Al-Bahrany. Pak.J.Biol.Sci. 5(12):1294-1296. (2002). 2-AS Moffat. Science. 271:1497-1506. (1996). 3-, PM Hasegawa; RA Bressan; S. Handa and AK, Handa. Hort. Science. 19:371-377. (1984). 4-AT Abdel-Raheem; AR, Ragab; ZA ...
The gradual and sudden additions of sodium chloride salt and the role of proline in minimizing their effects on two wheat (Triticum aestivum L.) cultivars cultured in vitro
Thi-Qar University Journal for Agricultural Researches, 2016
Effect of Sorbitol on Callus Induction and Somatic Embryos Regeneration in Two Local Wheat (Triticum aestivum L.) Cultivars
Journal of Basrah Researches (Sciences), 2013
A series of experiments were conducted in Plant Two local wheat varieties cultivars named Dor29 a... more A series of experiments were conducted in Plant Two local wheat varieties cultivars named Dor29 and Fateh were evaluated to determine the impact of 0, 10, 20 and 30 g.lP-1 P sorbitol for callus induction and somatic embryos regeneration. Mature seeds of the tested cultivars were selected as explants for callus induction and regeneration on MS medium with Gambrog vitamins containing 30 g.lP-1 P sucrose, 5 g.lP-1 P agar, 2 mg.lP-1 P BA and 1 mg.lP-1 P 2,4-D. Results were showed that sorbitol at 20 g.lP-1 P had a significant effect on callus fresh weight; mean globular somatic embryos number with no significant difference between the two cultivars under investigation. The obtained results from our study suggested that the optimal callus induction medium was MS + Gambrog vitamins + 30 g.lP-1 P sucrose + 20 g.lP-1 P sorbitol + 2 mg.lP-1 P BA + 1 mgP-P 2,4-D + 5 g.lP-1 P agar ,while maximum number of somatic embryos formed at the same previous medium with the amition of BA and 2,4-D.
Journal of Biology, Agriculture and Healthcare, 2017
Laboratory experiment using hydroponic culture system was conducted at plant physiology laborator... more Laboratory experiment using hydroponic culture system was conducted at plant physiology laboratory, Department of Biology, College of Education for Pure Sciences, University of Basrah, in order to study the role of seeds soaking with salicylic acid (SA) and the effect of PEG8000 induced water stress on germination and early seedling growth stages of maize ( Zea mays L.) cv. Baghdad. Seeds were soaked with three concentrations of SA (0.1,0.3 and 0.5 mM) in addition to control treatment with distilled water, while PEG8000 were used at concentrations of 0% (distilled water) 1%, 2%, 3%, 6%, 9% and 12%. The data was recorded on various parameters like seeds germination percentage, seedlings roots and shoot length and seedlings roots and shoot fresh and dry weight.The experiment was designed as completely randomized factorial with three replicates for each treatment. Results indicated that SA at 0.5 mM caused a significant increase in germination percentage roots and shoot length, roots...
The effect of seeds pretreatment with thiourea solution on salt stress tolerance in wheat plant (... more The effect of seeds pretreatment with thiourea solution on salt stress tolerance in wheat plant (cultivar Ebba-99) was studied. Seeds pretreated with 6 mmol/L of thiourea in addition to distilled water (control) for 6 hours, then the seeds was cultivated after during in pots contain on mixture river sand and peat. The seedling was exposed after two weeks from date of germination for induced salt stress with NaCl at concentrations (50, 100, 150 mmol) for 8 weeks. Salt stress caused significant decrease in growth of shoot height, leaf area, fresh and dry weights, relative water content (RWC) and significant increase activity of CAT, SOD and APX. The salt stress caused significant decrease in concentration of Ascorbic Acid while did not record significant difference in concentration both of Glutathione and Carotene, Lipid peroxidation (MDA), Proline accumulation significantly increased under salt stress conditions. Thiourea application by seeds soaking led to improvement in some of gro...
Effect of water stress on callus induction from shoot tips of date palm (Phoenix dactylifera L.) cv. Bream cultured in vitro
The results indicate that using PEG 3000 with previous concentrations caused significant increase... more The results indicate that using PEG 3000 with previous concentrations caused significant increase in callus fresh weight when compared with nutrient medium free of PEG 3000 with no significant difference between the two concentrations; while there was a significant increase in somatic embryos produced by nutrient medium with 10% PEG 3000 compared with that having 20% PEG or control treatment free of PEG.
Plant Cell Reports, 1998
Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Sha... more Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer 'Santa Cruz' were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with sucrose. The optimal concentration of sucrose was 40 g • l-1. Medium containing 200 ml • l-1 coconut water together with 40 g • l-1 sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.
Muthanna Journal of Agricultural Sciences
The study was conducted at plant physiology laboratory, department of biology, college of educati... more The study was conducted at plant physiology laboratory, department of biology, college of education for pure sciences, university of Basrah, at the period from 2/6/2019 till 1/11/2019 in order to evaluate the efficiency of alfalfa plant (Medicago sativa L.) for bioaccumulation of lead ions and to study the role of the chelating agent ethylene diamine tetraacetic acid (EDTA) in the accumulation process.Seeds of local variety of alfalfa plant were planted in plastic pots (16x16) filled with a mixture of sand and peat moss at ratio of 1:1 on volume basis. Lead as lead nitrate (Pb(NO3)2) was added alone to the pots at concentrations of 0 (full strength Hoagland nutrient solution), 0.25, 0.5 and 0.75 mM or in interaction with EDTA at concentrations of 0, 5 and 10 mM. The experiment continued for sixteen weeks after which the following results were estimated:Increasing lead nitrate concentrations or its interactions with EDTA cased a significant increase in lead ions accumulation in alfal...
Journal of Basrah Researches ((Sciences)), 2021
Model organisms are loadstar to the workers in the scientific field, because of its features such... more Model organisms are loadstar to the workers in the scientific field, because of its features such as small of chromosomes numbers or short life cycle, in addition it have a complete genomic map. Arabidopsis thaliana L. is a member of these groups. This plant is not its exists in Iraqi flora. And because of the difficult and costs of importing seeds from certificated suppliers, this study aimed to put a rapid and cost effective protocol for planting A. thaliana in Iraqi laboratories ambient and make it available for plant biology researchers.
Indian Journal of Ecology, 2021
The present study was aimed to evaluate the role of exogenous application for hydrogen peroxide d... more The present study was aimed to evaluate the role of exogenous application for hydrogen peroxide during soaking of seeds on tolerance of salt stress for wheat plant cultivar Ebba-99. The soaking of seeds with H O was carried out at 1.5 mmol concentration in addition to 2 2 distilled water (control) for 6 hours and the seeds were sown in pots contain on mixture river sand and peat. The seedling was exposed after two weeks from date of germination for induced salt stress with NaCl at different concentrations (50, 100, 150 mmol) for 8 weeks. The salt stress led to significant decrease in shoot height, leaf area, fresh and dry weight, relative water content and significant increase activity of catalase (CAT) and superoxide dismutase (SOD) at 150 mmol concentration, while increase was observed in activity of ascorbate peroxidase (APX) at 50 mmol concentration. The salt stress caused significant decrease in concentration of ascorbic acid but with no difference in concentration of glutathione and carotene. Salt stress caused significant increase in concentration of proline and malondialdehyde (MDA) content and increase of electrolyte leakage. The pretreatment of seeds with H O led to improvement in some of growth parameters (leaf area and fresh weight) and 2 2 improvement in enzymatic antioxidants (CAT, SOD, APX) and also in enhancement of non-enzymatic antioxidants(carotene, glutathione, ascorbic acid) levels and reduction in MDA content and electrolyte leakage.
Plant Archives Vol. 20, Supplement 2, 2020 pp. 717-722 , 2020
The effect of seeds pretreatment with thiourea solution on salt stress tolerance in wheat plant (... more The effect of seeds pretreatment with thiourea solution on salt stress tolerance in wheat plant (cultivar Ebba-99) was studied. Seeds pretreated with 6 mmol/L-1 of thiourea in addition to distilled water (control) for 6 hours, then the seeds was cultivated after during in pots contain on mixture river sand and peat. The seedling was exposed after two weeks from date of germination for induced salt stress with NaCl at concentrations (50, 100, 150 mmol) for 8 weeks. Salt stress caused significant decrease in growth of shoot height, leaf area, fresh and dry weights, relative water content (RWC) and significant increase activity of CAT, SOD and APX. The salt stress caused significant decrease in concentration of Ascorbic Acid while did not record significant difference in concentration both of Glutathione and Carotene, Lipid peroxidation (MDA), Proline accumulation significantly increased under salt stress conditions. Thiourea application by seeds soaking led to improvement in some of growth parameters, antioxidants system, reduction in MDA content and electrolyte leakage.
The effect of seawater salinity on callus growth, sodium and potassium concentrations, proline ac... more The effect of seawater salinity on callus growth, sodium and potassium concentrations, proline accumulation and some antioxidant enzymes ( ascorbate peroxidase, catalase and superoxide dismutase) activity in the callus of tomato (Lycopersicon esculentum Mill.) cv. Super Marmmande were investigated. Seeds of tomato were used as explant and cultured using Pyrex test tubes (150x20 mm) on MS medium with Gamborg vitamins, sucrose, 2,4-D, BA and soldifies with agar, for six weeks, to induce the callus formation and to get a considerable amount of callus to be used in the upcoming experiment. Seawater was diluted with distilled water and added to the medium at three concentrations (20%, 30% and 40%), 100 mg of the induced callus were cultured on the same previous medium for another six weeks to evaluate the effects of seawater salinity on growth and some biochemical parameters. Results indicated that high seawater concentration (40%) caused a significant decrease in callus fresh weight and potassium ion concentration, while a significant increase noticed in sodium ion concentration, proline accumulation, and the activity of antioxidant enzymes in the callus.
Journal of Biology, Agriculture and Healthcare , 2017
Laboratory experiment using hydroponic culture system was conducted at plant physiology laborator... more Laboratory experiment using hydroponic culture system was conducted at plant physiology laboratory, Department of Biology, College of Education for Pure Sciences, University of Basrah, in order to study the role of seeds soaking with salicylic acid (SA) and the effect of PEG8000 induced water stress on germination and early seedling growth stages of maize (Zea mays L.) cv. Baghdad. Seeds were soaked with three concentrations of SA (0.1,0.3 and 0.5 mM) in addition to control treatment with distilled water, while PEG8000 were used at concentrations of 0% (distilled water) 1%, 2%, 3%, 6%, 9% and 12%. The data was recorded on various parameters like seeds germination percentage, seedlings roots and shoot length and seedlings roots and shoot fresh and dry weight.The experiment was designed as completely randomized factorial with three replicates for each treatment. Results indicated that SA at 0.5 mM caused a significant increase in germination percentage roots and shoot length, roots and shoots fresh and dry weight while PEG8000 caused a significant decrease especially at its high concentrations (6% and 9%) in all the previous parameters. Interaction between SA at 0.5 mM and PEG had improved significantly germination percentage, shoot and roots length, shoot and roots fresh and dry weight so it had a positive significance effect on increasing the drought tolerance in maize.
Basrah Resch.J.(Sciences), 2013
A series of experiments were conducted in Plant Two local wheat varieties cultivars named Dor29 a... more A series of experiments were conducted in Plant Two local wheat varieties cultivars named Dor29 and Fateh were evaluated to determine the impact of 0, 10, 20 and 30 g.lP-1 P sorbitol for callus induction and somatic embryos regeneration. Mature seeds of the tested cultivars were selected as explants for callus induction and regeneration on MS medium with Gambrog vitamins containing 30 g.lP-1 P sucrose, 5 g.lP-1 P agar, 2 mg.lP-1 P BA and 1 mg.lP-1 P 2,4-D. Results were showed that sorbitol at 20 g.lP-1 P had a significant effect on callus fresh weight; mean globular somatic embryos number with no significant difference between the two cultivars under investigation. The obtained results from our study suggested that the optimal callus induction medium was MS + Gambrog vitamins + 30 g.lP-1 P sucrose + 20 g.lP-1 P sorbitol + 2 mg.lP-1 P BA + 1 mgP-P 2,4-D + 5 g.lP-1 P agar ,while maximum number of somatic embryos formed at the same previous medium with the amition of BA and 2,4-D.
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Papers by Luma Abdul-Qadir