Papers by Fatima Zohra El kadi
Azerbaijan Medical Journal, 2025
In an attempt to better understand the role of medicinal plants in the treatment of diabetes, an ... more In an attempt to better understand the role of medicinal plants in the treatment of diabetes, an ethnobotanical survey was conducted in the city of Sidi Bel Abbes among dozens of diabetic patients. The results obtained by 90 patients allowed us to identify 20 medicinal plants and 11 ethnobotanical family, based on the statistical analyses which were conducted using RStudio software a phytochemical study was achieved on the most cited plants representing, Olea europaea L cited by 20 individual (22.22 %) Rosmarinus Officinalis cited by 12 individual (13, 33%) and Artemisia herba Alba Asso cited by 6 individuals (6.66%). The results of qualitative phytochemical analysis revealed the presence of such active substances as flavonoids, tannins, alkaloids and phenols, almost no side effects were associated with the use of medicinal plants and concluded that these plants can be a natural source of antioxidant compounds of high importance.
AgroLife Scientific Journal, 2025
Opuntia ficus-indica (OFI) belongs to the Cactaceae family grown in arid regions, including Alger... more Opuntia ficus-indica (OFI) belongs to the Cactaceae family grown in arid regions, including Algeria. Various parts of OFI have been widely used in different countries for their therapeutic properties. Due to the lack of scientific evidence, the consumption of OFI cladodes remains still limited. Therefore, the present study aimed to evaluate the physicochemical and phytochemical properties of OFI cladodes from various harvesting areas in Algeria. Qualitative phytochemicals analysis was measured by following the standard methods, antioxidant activity was measured by the DPPH method, and quantification of secondary metabolites and ferric reducing antioxidant power (FRAP) tests were conducted using spectrophotometric methods respectively. The study revealed that cladodes from Medea station showed a significant yield, however, the content of ash, lipids, and potassium significantly differed from one region to another. While compared to the other stations, the phytochemical study showed that the ethanolic extract of cladodes from the Ain Temouchent station had significant levels of total polyphenols and flavonoids, hydrolyzable tannins and condensed tannins, steroids, alkaloids, and triterpenoids, respectively. Moreover, all of the extracts from different regions contained a significant amount of strong antioxidant activity. It can be concluded that the OFI cladodes contain a significant amount of phytochemicals and strong antioxidant activities, which could lead to new opportunities in various sectors including health, cosmetic, and food sectors.
African Journal of Biological Sciences, 2024
Abstract
The incidence of preeclampsia (PE) is associated with numerous risk
factors that are cla... more Abstract
The incidence of preeclampsia (PE) is associated with numerous risk
factors that are classified as high and moderate risk factors. In this
context, we carried out this study to determine the potential risks of PE
and to highlight those associated with the severity of the disease. This
is a case-control study of 200 pregnant women, groups of 100
preeclamptic (PG) and 100 non-preeclamptic (NPG) admitted between
March 2022 and January 2023 at the Maternity Ward of Sidi Bel Abbes
(Algeria). Data were analyzed using SPSS for Windows version 20.
Frequencies and percentages were calculated for categorical variables;
the Chi-square test was performed to compare between the two groups;
the risk factors and the odds ratio were identified using binary
regression. The current study showed that the development of PE was
associated with several risk factors, in particular renal disease, BMI ≥
35 and represented potential risk factors for the development of PE
with an a OR of (21.65, 95% CI; 2.56-182.75) and (18.47, 95% CI;
4.37-78.01), respectively. Furthermore, a history of PE and gestational
hypertension were potential risk factors and contributed to the
development of the severe form of PE with an aOR of (6.55, 95%
CI;2.78-15.43) and (7.32, 95% CI; 1.60-33.35); respectively
African Journal of Biological Sciences, 2024
Psoriasis is an immune-mediated inflammatory skin condition affecting 2-3% of the global populati... more Psoriasis is an immune-mediated inflammatory skin condition affecting 2-3% of the global population, often contributing to various comorbidities. This study aimed to investigate the profile, severity, associations, forms, treatment strategies, and quality of life in psoriasis patients in Oran, Algeria. Data were retrospectively collected over seven years (2016-2022) from two dermatology departments in Oran. Data analysis was performed using IBM SPSS Statistics 20. From 282 patients, 55.3% were female, with disease onset primarily between 18-30 years. Psoriasis was more common in urban residents (83.7%). High blood pressure (16.2%) was the most frequent comorbidity, and 24.2% had a family history of psoriasis. Psychological stress was a triggering factor in 58.9% of cases. Psoriasis vulgaris was most prevalent (70.2%). Topical treatments were the most common (57.4%). The severity index was moderate (10.9±0.40), with a moderate to severe impact on quality of life (11.3±0.48). PASI correlated with family history of autoimmune/metabolic diseases (P=0.046; P=0.043), comorbidities like diabetes and hypertension (P=0.000), and lifestyle factors (P=0.000). PASI was strongly correlated with the quality of life (P=0.000, r=0.604) and inflammation markers (CRP, ESR).Psoriasis significantly influence health, affecting both genders and various age groups. Its etiology and development of comorbidities remain poorly defined.
Scientific Journal of King Faisal University: Basic and Applied Sciences, 2024
This research investigated the influence of a hyperlipidic diet in Wistar rats and the therapeuti... more This research investigated the influence of a hyperlipidic diet in Wistar rats and the therapeutic effect of Brassica rapa powder (BrP). A hyperlipidic diet was administered followed by oral supplementation of BrP (5%) for 28 days. The BrP supplementation caused a decrease in weight (-41%), glycaemia (-12%), creatinemia (-51%), aspartate aminotransferase (AST) level (-80%), and cholesterolaemia (-25%) associated with an increase in HDLc level (+59%). However, the hepatic lipid profile showed a decrease in phospholipids (PL) (-59%) and total cholesterol (TC) (-67%). In addition, oxidative stress assessment showed a decrease in the serum (-22%), renal (-30%) and adipose (-8%) levels of TBARS. Furthermore, BrP supplementation boosted glutathione (GSH) protective activity in adipose tissue with a maximum of +49%. In conclusion, Brassica rapa enhances the serum and tissue balance of metabolic and antioxidant status.
THE ANNALS OF “VALAHIA” UNIVERSITY OF TARGOVISTE, 2024
Three species of Allium cepa (red, purple, yellow) were evaluated for their secondary metabolite ... more Three species of Allium cepa (red, purple, yellow) were evaluated for their secondary metabolite content, free radical scavenging activity (DPPH), and ferric reducing ability (FRAP). Our findings confirm that the dry matter content in all varieties ranged from 12.3% to 18.1%, rendering them suitable for extended storage. The ethanolic extract of red onion (EERO) displayed the highest yield (3.86%). Similarly, the levels of total phenols (TPC), total flavonoid content (TFC), hydroxycinnamic acid (HTC), and cysteine-containing peptide (CTC) varied between 0.14 and 0.27 mg/g GAE, 0.20 and 0.29 mg/g QE, 0.25 and 0.29 mg/g GAE, and 0.015 and 0.102 mg CE/g DW, respectively." Furthermore, ethanolic extract of yellow onion (EEYO) demonstrated a significant iron reduction capacity (0.75 ± 0.06 mM Fe2+) and an IC50 of 0.159 ± 0.18 mg/ml, indicating enhanced antioxidant potential.
FARMACIA , 2023
Many developing countries employ medicinal plants for the treatment of kidney stone dissolution. ... more Many developing countries employ medicinal plants for the treatment of kidney stone dissolution. Patients consume aqueous extracts without precise knowledge of the plant's composition. This study aimed to expand the phytochemical knowledge of traditional herbal remedies and evaluate their constituents for the dissolution and inhibition of kidney stones. The herbal plants selected were Ammi visnaga, Nigella sativa, Berberis vulgaris, Haloxylon scoparium, Atriplex halimus and Arthrophytum schmittianum. An extract of each plant was prepared by the infusion method in physiological serum; the extract was filtered and put in the presence of calculi for eight weeks under magnetic agitation. The calculi were weighed biweekly after undergoing a drying process at 40°C for 18 hours, and the pH of the solution was consistently determined. Ammi visnaga, Nigella sativa and Haloxylon scoparium caused significant mass loss and thus had a considerable influence on the dissolution and in vitro inhibition of calcium calculi. In conclusion, this study shows that plants containing coumarins, alkaloids, triterpenes and tannins affect the dissolution and inhibition of kidney stone calcium oxalate. Rezumat Plantele medicinale se utilizează pentru tratamentul calculilor renali, însă pacienții consumă fitopreparatele fără a cunoaște cu exactitate compoziția acestora. Studiul a evaluat constituenții fitopreparatelor pentru dizolvarea și inhibarea calculilor renali. Plantele medicinale selectate au fost Ammi visnaga, Nigella sativa, Berberis vulgaris, Haloxylon scoparium, Atriplex halimus și Arthrophytum schmittianum. A fost preparat un extract din fiecare plantă prin metoda infuziei în ser fiziologic; extractul a fost filtrat și evaluat în prezența calculilor timp de opt săptămâni sub agitație magnetică. Calculii au fost cântăriți de două ori pe săptămână, după ce au fost supuși unui proces de uscare la 40°C timp de 18 ore, iar pH-ul soluției a fost determinat în mod constant. Ammi visnaga, Nigella sativa și Haloxylon scoparium au provocat pierderi semnificative de masă și, prin urmare, au avut o influență considerabilă asupra dizolvării și inhibării in vitro a calculilor de calciu. În concluzie, acest studiu arată că produsele vegetale care conțin cumarine, alcaloizi, triterpene și taninuri influențează dizolvarea și inhibarea calculilor renali de oxalat de calciu.
THE ANNALS OF “VALAHIA” UNIVERSITY OF TARGOVISTE, 2023
In order to study the adaptive behavior of two tomato varieties (Solanumlicopersicum L.) Safa and... more In order to study the adaptive behavior of two tomato varieties (Solanumlicopersicum L.) Safa and Assila subjected to salt stress with concentrations of (50, 100, 150 and 200 mM) in (NaCl+CaCl2) during the growth phase, a morphological (number of leaves, stem length, stomatal density, length of the main root axis) and physio-biochemical (relative water content, chlorophyll pigments content, soluble sugars content) evaluation was conducted. Our results confirm the sensitivity of both varieties to applied salt stress; this sensitivity was manifested by a statistically significant decrease in: number of leaves, length of the stem and the contents of chlorophyll a and total chlorophyll. While, the soluble sugars content at the leaf level increased associated with an increase stomatal density on both ventral and dorsal epiderms. On the other hand, the ratio of root dry weight and shoot dry weight (RDW/SDW) was decreased with an elongation of the main root according to the different doses of salts.
HPLC-MS Analysis and evaluation of Antioxidant and Anti-Inflammatory Potential of Cinnamomum cassia Extract, 2023
In the current decade, growing interest has been in using herbs and spices as antioxidants and an... more In the current decade, growing interest has been in using herbs and spices as antioxidants and anti- inflammatory agents. This study aims to investigate the chemical composition and evaluation of the antioxidant effect of Cinnamomum cassia extract. First, the chemical composition of C. cassia
methanolic extract was determined using the HPLC-MS method. Then, the antioxidant potential was evaluated using the DPPH scavenging and total antioxidant capacity (TAC) methods. For the anti-inflammatory potential, three methods were performed: Membrane stabilization assay and
inhibition of protein denaturation. five compounds were identified in the methanolic extract: Quercetin-O-hexoside, Quercetin-O-pentoside, Kaempferol-O-coumaroyl, Diosmin and Cinnamic acid. The methanolic extract exhibited the highest scavenging activity recording activity
of 93.08 ± 0.05% at 1000 μg/ mL concentrations compared to the aqueous extract (77.03 ± 0.07%). The methanolic extract also presented the highest value of TAC (208.85 mg AAE/g) compared to the aqueous extract (199.56 ± 0.47 mg AAE/g). Methanolic extract presented the highest percentage for HRBC protection (87.27 ± 0.09%) at concentrations of 1000 μg/ mL. For the
protein denaturation method, the aqueous extract showed an inhibition percentage of 32.86 ± 4.66% against methanolic extract (24.56 ± 4.11%). Cinnamomum cassia manifested an important free radical scavenging activity and anti-inflammatory potential. Therefore, it can be considered
an alternative treatment against oxidative stress and biomolecules damages.
AGROFOR International Journal, 2023
The objective of our study was the antifungal power test of the pomegranate peel aqueous extract ... more The objective of our study was the antifungal power test of the pomegranate peel aqueous extract PPE, on a phytopathogenic fungal strain of tomato "Fusarium oxysporum f.sp.radicis-lycopersici". For this purpose, a test was carried out, by the direct contact method in PDA potato dextrose agar medium, on the phytopathogen, in different concentrations of PPE (1%, 2%, 3%, 5%). Antifungal activity was evaluated by the estimation of the mycelial growth, the mycelial inhibition rate and the spore count. Our results showed a remarkable and highly significant antifungal activity against the studied phytopathogen, an inhibition index of 100% with the 3% and 5% concentrations was noted. The effectiveness of PPE could be the subject of investigation and exploitation in the integrated control of the tested fungus that causes much damage to the tomato crop.
Egyptian Academic Journal of Biological Sciences C. Physiology & Molecular Biology, 2023
Bacteria are attracting the interest of worldwide investors; their use is
interesting in several ... more Bacteria are attracting the interest of worldwide investors; their use is
interesting in several industrial fields, this organism produces a wide variety of extracellular enzymes, including proteases. The objective of this study was to evaluate the production of protease by different bacterial strains isolated from local marine samples collected from the Cap Rousseau beach in the Oran city, northwestern Algeria, 44 bacterial isolates were tested for protease production by cultivating them on skim milk agar medium. The proteolytic activities of all strains were tested using skim milk agar and gelatin agar. Among the 14 isolates that showed a significant hydrolysis diameter, two bacterial strains EC23 and EC2S3 demonstrated the highest potential for protease production and they were selected for further studies. In addition, the extracellular protease was examined using the fermentation production medium at 30°C for 48h, with a constant agitation of 150 rpm. The enzyme activity was determined under varying conditions of pH, incubation temperature, and salt concentration, using Sigma’s
Universal Protease Activity Assay. The enzyme from EC23 strain showed higher activity in all cases than the EC2S3 strain, which indicated that it was the most ideal organism for enzyme production.
Egyptian Academic Journal of Biological Sciences C. Physiology & Molecular Biology, 2022
Diabetes mellitus is a chronic metabolic disorder, which can cause damage
to multiple organs; one... more Diabetes mellitus is a chronic metabolic disorder, which can cause damage
to multiple organs; one of the long-term effects of diabetes is diabetic nephropathy; oxidative stress is thought to play a role in the development of diabetic nephropathy, as it can induce an inflammatory response and damage cells by disrupting their redox homeostasis; The study's goal was to assess the levels of oxidative damage and antioxidant protection in diabetic nephropathy patients. During routine medical examinations, 105 patients and 101 controls were selected; in serum, the reaction of thiobarbituric acid with malondialdehyde can be quantified by measuring the intensity of a pink color produced at 95°C. The same method is used to assess the degree of oxidation of free-cholesterol-LDL; total plasma antioxidant status was
determined by a colorimetric enzymatic peroxidase method. Erythrocyte superoxide dismutase activity was determined by xanthine oxidase, catalase and glutathione peroxidase activities were measured by a colorimetric method; ascorbic acid was measured in an acidic solution using a colorimetric technique at a wavelength of 465 nanometers. Retinol and tocopherol were isolated from serum by liquid-liquid extraction and immediately injected into a high-performance liquid chromatography
system. The results show that patients had 1.77 times higher serum TBARS, 2.77 times higher free-cholesterol-LDL-TBARS, 1.43 times lower total antioxidant status, 1.30 times lower superoxide dismutase activity, 1.19 times lower catalase activity, 1.28 times lower glutathione peroxidase activity, 1.83 times lower α-tocopherol, 1.33 times lower ascorbic acid, and 1. 15 times lower retinol than controls; the results of the present research also demonstrate that significant differences were observed between patients and controls in blood urea (p=.013), serum creatinine (p=.007), uric
acid (p=.015), albumin (p=.018), and glomerular filtration rate (GFR) (p=.006). In diabetic nephropathy, there is a negative correlation between glomerular filtration rate and oxidant status parameters, while there is a positive correlation with antioxidant status indicators
Egyptian Academic Journal of Biological Sciences C. Physiology & Molecular Biology, 2022
The current study's main goal is to assess the antioxidant activity, secondary metabolites, and p... more The current study's main goal is to assess the antioxidant activity, secondary metabolites, and physicochemical quality indices of linseed (Linum usitatissimum L.) oily extract (Luo), along with the effect of linseed oil addition on the preservation of beef mince during refrigerated storage at 6°C. The oil was extracted by the Soxhlet method from Linum usitatissimum seeds with a yield of 53.96%, and its physicochemical and biological quality were assessed. The results showed that the oil met international standards, with values of refractive index (1.4764), acid index (0.40± 0.06 mg KOH/g), saponification index (177± 0.66 mg KOH/g), ester index (176.6 ± 0.66 mg KOH/g), and peroxide index (1.5 ± 0.66 meq d'O2/kg). Secondary metabolite analysis revealed that the concentrations of phenolic compounds, flavonoids, flavonols, and condensed tannins were (0.50±0.04mg GAE/ml oil), (0.39±0.02mg QE/ml oil), (0.038±0.003mg QE/ml oil), and (0.036±0.002 mg CE/ml oil), respectively. However, IC50 value was 0.54±0.06 mg/ml, demonstrated a remarkable ability to scavenge the 2,2-diphenyl-1picrylhydrazyl radical (DPPH-). Likewise, pH of the ground meat enriched with flaxseed oil was extremely high compared to the control meat. Ultimately, Linum usitatissimum oil was abundant in bioactive and antioxidant molecules. This suggests that flaxseed oil could be used to promote the food industry as a preservative.
Egyptian Academic Journal of Biological Sciences C. Physiology & Molecular Biology, 2022
The objective of the present study is to the total phenolic compound
determination, as well as th... more The objective of the present study is to the total phenolic compound
determination, as well as the in vitro evaluation of methanolic and ethanolic
extracts antimicrobial activity of the medicinal plant Mentha rotundifolia L.
against pathogenic strains. The Follin-Cioacalteu method was used to
determine the total polyphenols, while the flavonoids were estimated
according to the colorimetric method with aluminum chloride. In addition,
the determination of condensed tannins is performed by the vanillin method.
The antimicrobial activity is tested on five pathogenic bacterial strains, two of
which are Gram-negative (Escherichia coli ATCC 25922, and Pseudomonas
aeruginosa ATCC 27853), three Gram-positive (Bacillus cereus ATCC
11778, Staphylococcus aureus ATCC 25923 and Micrococcus leutus ATCC
4698), and one yeast (Candida albicans ATCC 26790), by the diffusion on
solid medium and microdilution methods. The ethanolic extract of round leaf
mint from the Bechar region is very rich in polyphenols and flavonoids
(401.75 ± 0.32 mg GAE/g, 549.75 ± 1.23 mg QE/g) compared to the
methanolic and hydroalcoholic extracts of this plant from the Sidi-Bel-Abbes
region. The results of the obtained well method showed that all bacterial
strains exhibited sensitivity to the ethanolic extract of Mentha rotundifolia L.
from the Bechar region, with inhibition zones up to 21,67 ± 0,57 mm and
minimum inhibitory concentrations (MIC) varying between 06.25 mg/ml and
100 mg/ml, The effect of the other extracts on the tested strains was less
significant, the methanolic extract was the least effective. These results
demonstrate that the Mentha rotundifolia L. ethanolic extract has a great
potential of secondary metabolites with antimicrobial activity.
JOURNAL of Horticulture, Forestry and Biotechnology, 2022
The objective of this study is to carry out a phytochemical and biological characterization of Ha... more The objective of this study is to carry out a phytochemical and biological characterization of Hawthorn (Crataegus oxyacantha). For this purpose, the Crataegus oxyacantha extracts prepared from leaves, fruits and bark of were subjected to a qualitative and quantitative phytochemical screening ; as well as an estimation of the level of mineral salts, namely Na and K, and an evaluation of the antioxidant profile via the DPPH test. Our results showed the presence of highly antioxidant substances (flavonoids, condensed tannins). The quantitative estimation of total polyphenols (Folin-ciocalteau), total flavonoids (aluminum trichloride) and condensed tannins (vanillin) ,showed that the aqueous extract of the bark is the richest extract in these compounds. In addition, this study confirmed the richness of our plant in Na and P, especially the fruit with Na + = 712.79 mg/l and the leaf with K + = 2331.73 mg/l. According to the DPPH test, the extract of the bark is the most active. This antioxidant power provides to Crataegus oxyacantha species its therapeutic properties, namely cardiotonic.
Acta Agriculturae Serbica, 2022
Beta-casein (-CN) is a major dairy protein subject to preferential degradation during storage. O... more Beta-casein (-CN) is a major dairy protein subject to preferential degradation during storage. Our study aimed to isolate β-casein from bovine milk under dissociative conditions by the electrophoretic technique using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and optimize an immunochemical technique such as Mancini radial immunodiffusion in order to monitor the evolution of this milk protein during storage. For this purpose, a series of physicochemical and bacteriological analyses revealed conformity with international standards. This allowed us to use quality milk for more detailed studies of proteins, in particular β-casein, which was selected as a marker of milk protein quality. The total casein of mixed milk samples was isolated and then submitted to electrophoretic separation. Various combinations of acrylamide and bisacrylamide were used and the one corresponding to the ratio 8 g / 230 mg was retained. A total casein deposit of 833 µg provided an adequate β-casein concentration (300 µg) for consistent animal immunization. Thirty Wistar rats were subjected to an immunization protocol for 35 days. The obtained antisera were used to optimize the Mancini technique and to assay β-casein in our samples at different storage times ranging from 3 h to 69 h/+4°C. A decrease in β-casein content from 0.85 mg ml-1 to 0.32 mg ml-1 was detected. These results demonstrate the vulnerability of β-casein at low temperatures and provide information on the origin of milk and its application for dairy or cheese production. In addition, our study confirms the usefulness of immunochemical techniques such as the Mancini test in the determination of β-casein.
International Journal of Minor Fruits, Medicinal and Aromatic Plants., 2022
The coffee grounds CG are considered as a solid residue generated by consumers of ground coffee.F... more The coffee grounds CG are considered as a solid residue generated by consumers of ground coffee.For this purpose, three samples of dry coffee grounds DCG and its oily extract (Sample 1: Arabica, Sample 2: Robusta, Sample 3: mixture of two varieties) were subjected to a physicochemical characterization, quantification of primary and secondary metabolites and the mineral salts determination.The results show that the DCG of the three samples is a good source of carbohydrates with a max of 65.31±0.67%. In fact, the oil DCG extraction provided a yield with a maximum of 15.01±0.9%. The obtained values for the different physicochemical quality indices (refractive index RI , acidity index AI , ester index EI , saponification index SI and peroxide index PI),showed the conformity of the studied oils to the standards. Laquantification of secondary metabolites showed that the oily extract of mixture of two varieties is the richest in polyphenols with a value of 50.28±13.32 mg GAE/gr, on the other hand, the Robusta variety was found to be the richest in flavonoids (337.12±22.66 mg CE/gr).
Journal of Agroalimentary Processes and Technologies , 2022
The tolerance of two durum wheat varieties was tested under different levels of salinity using an... more The tolerance of two durum wheat varieties was tested under different levels of salinity using an experimental approach in the laboratory. The results show that the Vitron variety performs significantly better than the Simeto variety despite the fact that the tolerance threshold of both varieties to NaCl is lower than 2 gr/ l. The germination capacity of the Simeto variety was significantly lower than that of the Vitron variety which germinated in a relatively average time of 4 days and recorded the highest germination rates, 73% for seeds watered with distilled water, 53% at 2g/l , and 33% at 5g/l of NaCl , a significant decrease in coleoptile and root length was recorded for both varieties, the Simeto variety recorded significantly higher levels of proline than the Vitron variety, 0.346 µg/ml vs 0.280 µg/ml under 2 g/l , respectively
gyptian Academic Journal of Biological Sciences C. Physiology & Molecular Biology, 2022
Psoriasis is an autoimmune and inflammatory disease that affects
the skin cells and is characteri... more Psoriasis is an autoimmune and inflammatory disease that affects
the skin cells and is characterized by the presence of plaques covered with
squamous. The aim of this study is to determine the epidemiological,
ethiopathogenic and therapeutic profile of a psoriatic population. To this
purpose, we conducted a retrospective clinical study on 87 psoriatic
patients, the parameters under consideration are: sex, age, socio-economic
level, personal and family history, disease history (age and site of
beginning, triggering factors and symptoms of beginning, type of
psoriasis) and therapeutic strategy. Our results showed that psoriasis
affects both sexes equally, as it can appear at any age, although it
manifests itself preferentially between 21 and 60 years-old , especially in
the following populations: urban (60.90%), smokers (44.8%), those with
an autoimmune and/or metabolic pathology (50.5%), family character of
psoriasis (9.2%). Similarly, medication (32%), stress (17.2%) and psycho-
affective shock (11.4%) are the major triggering factors. This pathology is
mainly manifested by pruritus (41.4%), of which (71%) of patients have a
vulgar type. The therapeutic strategy was rotational between the different
therapeutic weapons with a dominance of partial response (43.7%) to
therapies.
Universitatea”Vasile Alecsandri” din Bacău, 2022
The determination of bilirubin remains the
key examination to diagnose neonatal icterus and its
s... more The determination of bilirubin remains the
key examination to diagnose neonatal icterus and its
severity and on which the therapeutic decision is
based, The objective of our study is to detect the
choice method for the bilirubin determination .To
this effect, 100 cases of newborn babies with
mucocutaneous icterus at the Neonatology
Department of the maternity Hospital of the Sidi Bel
Abbés region (Algeria) were subjected to bilirubin
determination by diazoreaction manually by
spectrophotometry using the ERBA and Biomaghreb
reagents and automatically by means of an automaton
using the ERBA reagent. Our results showed that the
accelerating factor and the incubation time influence
the most sensitive fraction of bilirubin which is the
total bilirubin, Similarly, the cetrimide accelerant of
the ERBA reagent is more efficient than the DMSO
of the Biomaghreb reagent. In view of the advantages
of the automatic technique and the quality of the
reagent used, ERBA and its cetrimide booster, it is
chosen as the reference technique for the bilirubin
determination in neonates.
Uploads
Papers by Fatima Zohra El kadi
The incidence of preeclampsia (PE) is associated with numerous risk
factors that are classified as high and moderate risk factors. In this
context, we carried out this study to determine the potential risks of PE
and to highlight those associated with the severity of the disease. This
is a case-control study of 200 pregnant women, groups of 100
preeclamptic (PG) and 100 non-preeclamptic (NPG) admitted between
March 2022 and January 2023 at the Maternity Ward of Sidi Bel Abbes
(Algeria). Data were analyzed using SPSS for Windows version 20.
Frequencies and percentages were calculated for categorical variables;
the Chi-square test was performed to compare between the two groups;
the risk factors and the odds ratio were identified using binary
regression. The current study showed that the development of PE was
associated with several risk factors, in particular renal disease, BMI ≥
35 and represented potential risk factors for the development of PE
with an a OR of (21.65, 95% CI; 2.56-182.75) and (18.47, 95% CI;
4.37-78.01), respectively. Furthermore, a history of PE and gestational
hypertension were potential risk factors and contributed to the
development of the severe form of PE with an aOR of (6.55, 95%
CI;2.78-15.43) and (7.32, 95% CI; 1.60-33.35); respectively
methanolic extract was determined using the HPLC-MS method. Then, the antioxidant potential was evaluated using the DPPH scavenging and total antioxidant capacity (TAC) methods. For the anti-inflammatory potential, three methods were performed: Membrane stabilization assay and
inhibition of protein denaturation. five compounds were identified in the methanolic extract: Quercetin-O-hexoside, Quercetin-O-pentoside, Kaempferol-O-coumaroyl, Diosmin and Cinnamic acid. The methanolic extract exhibited the highest scavenging activity recording activity
of 93.08 ± 0.05% at 1000 μg/ mL concentrations compared to the aqueous extract (77.03 ± 0.07%). The methanolic extract also presented the highest value of TAC (208.85 mg AAE/g) compared to the aqueous extract (199.56 ± 0.47 mg AAE/g). Methanolic extract presented the highest percentage for HRBC protection (87.27 ± 0.09%) at concentrations of 1000 μg/ mL. For the
protein denaturation method, the aqueous extract showed an inhibition percentage of 32.86 ± 4.66% against methanolic extract (24.56 ± 4.11%). Cinnamomum cassia manifested an important free radical scavenging activity and anti-inflammatory potential. Therefore, it can be considered
an alternative treatment against oxidative stress and biomolecules damages.
interesting in several industrial fields, this organism produces a wide variety of extracellular enzymes, including proteases. The objective of this study was to evaluate the production of protease by different bacterial strains isolated from local marine samples collected from the Cap Rousseau beach in the Oran city, northwestern Algeria, 44 bacterial isolates were tested for protease production by cultivating them on skim milk agar medium. The proteolytic activities of all strains were tested using skim milk agar and gelatin agar. Among the 14 isolates that showed a significant hydrolysis diameter, two bacterial strains EC23 and EC2S3 demonstrated the highest potential for protease production and they were selected for further studies. In addition, the extracellular protease was examined using the fermentation production medium at 30°C for 48h, with a constant agitation of 150 rpm. The enzyme activity was determined under varying conditions of pH, incubation temperature, and salt concentration, using Sigma’s
Universal Protease Activity Assay. The enzyme from EC23 strain showed higher activity in all cases than the EC2S3 strain, which indicated that it was the most ideal organism for enzyme production.
to multiple organs; one of the long-term effects of diabetes is diabetic nephropathy; oxidative stress is thought to play a role in the development of diabetic nephropathy, as it can induce an inflammatory response and damage cells by disrupting their redox homeostasis; The study's goal was to assess the levels of oxidative damage and antioxidant protection in diabetic nephropathy patients. During routine medical examinations, 105 patients and 101 controls were selected; in serum, the reaction of thiobarbituric acid with malondialdehyde can be quantified by measuring the intensity of a pink color produced at 95°C. The same method is used to assess the degree of oxidation of free-cholesterol-LDL; total plasma antioxidant status was
determined by a colorimetric enzymatic peroxidase method. Erythrocyte superoxide dismutase activity was determined by xanthine oxidase, catalase and glutathione peroxidase activities were measured by a colorimetric method; ascorbic acid was measured in an acidic solution using a colorimetric technique at a wavelength of 465 nanometers. Retinol and tocopherol were isolated from serum by liquid-liquid extraction and immediately injected into a high-performance liquid chromatography
system. The results show that patients had 1.77 times higher serum TBARS, 2.77 times higher free-cholesterol-LDL-TBARS, 1.43 times lower total antioxidant status, 1.30 times lower superoxide dismutase activity, 1.19 times lower catalase activity, 1.28 times lower glutathione peroxidase activity, 1.83 times lower α-tocopherol, 1.33 times lower ascorbic acid, and 1. 15 times lower retinol than controls; the results of the present research also demonstrate that significant differences were observed between patients and controls in blood urea (p=.013), serum creatinine (p=.007), uric
acid (p=.015), albumin (p=.018), and glomerular filtration rate (GFR) (p=.006). In diabetic nephropathy, there is a negative correlation between glomerular filtration rate and oxidant status parameters, while there is a positive correlation with antioxidant status indicators
determination, as well as the in vitro evaluation of methanolic and ethanolic
extracts antimicrobial activity of the medicinal plant Mentha rotundifolia L.
against pathogenic strains. The Follin-Cioacalteu method was used to
determine the total polyphenols, while the flavonoids were estimated
according to the colorimetric method with aluminum chloride. In addition,
the determination of condensed tannins is performed by the vanillin method.
The antimicrobial activity is tested on five pathogenic bacterial strains, two of
which are Gram-negative (Escherichia coli ATCC 25922, and Pseudomonas
aeruginosa ATCC 27853), three Gram-positive (Bacillus cereus ATCC
11778, Staphylococcus aureus ATCC 25923 and Micrococcus leutus ATCC
4698), and one yeast (Candida albicans ATCC 26790), by the diffusion on
solid medium and microdilution methods. The ethanolic extract of round leaf
mint from the Bechar region is very rich in polyphenols and flavonoids
(401.75 ± 0.32 mg GAE/g, 549.75 ± 1.23 mg QE/g) compared to the
methanolic and hydroalcoholic extracts of this plant from the Sidi-Bel-Abbes
region. The results of the obtained well method showed that all bacterial
strains exhibited sensitivity to the ethanolic extract of Mentha rotundifolia L.
from the Bechar region, with inhibition zones up to 21,67 ± 0,57 mm and
minimum inhibitory concentrations (MIC) varying between 06.25 mg/ml and
100 mg/ml, The effect of the other extracts on the tested strains was less
significant, the methanolic extract was the least effective. These results
demonstrate that the Mentha rotundifolia L. ethanolic extract has a great
potential of secondary metabolites with antimicrobial activity.
the skin cells and is characterized by the presence of plaques covered with
squamous. The aim of this study is to determine the epidemiological,
ethiopathogenic and therapeutic profile of a psoriatic population. To this
purpose, we conducted a retrospective clinical study on 87 psoriatic
patients, the parameters under consideration are: sex, age, socio-economic
level, personal and family history, disease history (age and site of
beginning, triggering factors and symptoms of beginning, type of
psoriasis) and therapeutic strategy. Our results showed that psoriasis
affects both sexes equally, as it can appear at any age, although it
manifests itself preferentially between 21 and 60 years-old , especially in
the following populations: urban (60.90%), smokers (44.8%), those with
an autoimmune and/or metabolic pathology (50.5%), family character of
psoriasis (9.2%). Similarly, medication (32%), stress (17.2%) and psycho-
affective shock (11.4%) are the major triggering factors. This pathology is
mainly manifested by pruritus (41.4%), of which (71%) of patients have a
vulgar type. The therapeutic strategy was rotational between the different
therapeutic weapons with a dominance of partial response (43.7%) to
therapies.
key examination to diagnose neonatal icterus and its
severity and on which the therapeutic decision is
based, The objective of our study is to detect the
choice method for the bilirubin determination .To
this effect, 100 cases of newborn babies with
mucocutaneous icterus at the Neonatology
Department of the maternity Hospital of the Sidi Bel
Abbés region (Algeria) were subjected to bilirubin
determination by diazoreaction manually by
spectrophotometry using the ERBA and Biomaghreb
reagents and automatically by means of an automaton
using the ERBA reagent. Our results showed that the
accelerating factor and the incubation time influence
the most sensitive fraction of bilirubin which is the
total bilirubin, Similarly, the cetrimide accelerant of
the ERBA reagent is more efficient than the DMSO
of the Biomaghreb reagent. In view of the advantages
of the automatic technique and the quality of the
reagent used, ERBA and its cetrimide booster, it is
chosen as the reference technique for the bilirubin
determination in neonates.