Journal of Chromatography B-analytical Technologies in The Biomedical and Life Sciences, 2004
Scorpion venom are complex mixtures of peptides, known to cause impairment of ion-channel functio... more Scorpion venom are complex mixtures of peptides, known to cause impairment of ion-channel function in biological membranes. This report describes the separation of approximately 60 different components by high performance liquid chromatography and the characterization by Edman degradation and mass spectrometry of 26 peptides from the soluble venom of the Amazonian scorpion Tityus cambridgei. One of these peptides, named Tc48a, was fully characterized. It contains 65 amino acid residues, the C-terminal residue is amidated and it affects Na + -channels with a K d of about 82 nM. Furthermore, this report shows the thermo-instability of scorpion toxins subjected to electron spray ionization-mass spectrometry (ESI-MS). When a proline residue is located near the N-terminal region of the toxin, not stabilized by disulfide bridges, artificial components are generated by the mass spectrometer conditions, due to the cleavage of the peptide bond at the proline positions. This phenomenon was confirmed by using four model proteins (variable regions of immunoglobulins) studied by ESI-MS and matrix assisted laser desorption ionization-time of flight (MALDI-TOF)/MS.
Cualquier enfermedad en el hombre es el resultado de un proceso dinámico, en donde diferentes ele... more Cualquier enfermedad en el hombre es el resultado de un proceso dinámico, en donde diferentes elementos ambientales y características propias del hombre entran en interacción y concluyen con la ruptura del equilibrio llamado homeostasis. Al ser parte de un proceso continuo que se origina antes o durante la concepción del ser humano, la enfermedad responde al fenómeno de adaptación ecológica del organismos humano y a las condiciones sociales y económicas que facilitan o dificultan el proceso adaptativo.
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