SSO1450 – A CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated protein genes ( cas genes) are ubiquitous in archaea and eubacteria. It has been suggested that CRISPR and CAS proteins act as an immune system preventing the invasion of foreign genomic elements at the DNA level. The protein SSO1450 from Sulfolobus solfataricus ( Sso) P2 belongs to the CAS1 cluster which is one of the core protein clusters most frequently associated with CRISPR sequences. In this study we show that SSO1450 is a high-affinity nucleic acid binding protein. It binds DNA, RNA and DNA–RNA hybrid apparently sequence non-specific in a multi-site binding mode. Furthermore, SSO1450 promotes the hybridization of complementary nucleic acid strands.
- Publication:
-
FEBS Letters
- Pub Date:
- January 2009
- DOI:
- Bibcode:
- 2009FEBSL.583.1928H
- Keywords:
-
- CAS;
- CRISPR-associated;
- CASS;
- CRISPR-associated system;
- COG;
- cluster of orthologous groups;
- CRISPR;
- clustered regularly interspaced short palindromic repeats;
- DTT;
- dithiothreitol;
- EMSA;
- electrophoretic mobility shift assay;
- IPTG;
- isopropyl-β- d-thiogalactoside;
- K d;
- apparent dissociation constant;
- LB;
- Luria-Bertani medium;
- OD 600;
- optical density at 600 nm;
- PAGE;
- polyacrylamide gel electrophoresis;
- RNAi;
- RNA interference;
- SDS;
- sodium dodecylsulfate;
- Sso;
- Sulfolobus solfataricus;
- ss/ds;
- single/double-stranded;
- bp;
- base pair;
- nt;
- nucleotide;
- CRISPR-associated;
- CRISPR;
- Nucleic acid binding;
- SSO1450