Papers by Rosario Linacero
Somaclonal Variation In Plants Regenerated From Embryo Calluses In Rye (Secale Cerale L.)
De Gruyter eBooks, Dec 31, 1986
Tree nuts show nutritional properties and human health benefits. However, they contain allergenic... more Tree nuts show nutritional properties and human health benefits. However, they contain allergenic proteins, which make them harmful to the sensitive population. The presence of tree nuts on food labelling is mandatory and, consequently, the development of suitable analytical methodologies to detect nuts in processed foods is advisable. Real-time PCR allowed a specific and accurate amplification of allergen sequences. Some food processing methods could induce structural and/or conformational changes in proteins by altering their allergenic capacity, as well as produce the fragmentation and/or degradation of genomic DNA. In this work, we analysed by means of Real-time PCR, the influence of pressure and thermal processing through Instant Controlled Pressure Drop (DIC) on the detectability of hazelnut; pistachio and cashew allergens have been tested. The detection of targets in hazelnut, pistachio and cashew (Cor a 9, Pis v 1 and Ana o 1, respectively) is affected by the treatment, in different extent depending on the tree nut. Results are compared to those previously obtained by our group in the analysis of different treatments on the amplificability of the same targets. Reduction in amplificability is similar to that reported for some autoclave conditions. Our assays might allow detecting up to 1000 ppm of hazelnut, pistachio and cashew flours after being submitted to DIC treatment in food matrices.
Tree nuts show nutritional properties and human health benefits. However, they contain allergenic... more Tree nuts show nutritional properties and human health benefits. However, they contain allergenic proteins, which make them harmful to the sensitised population. The presence of tree nuts on food labelling is mandatory and, consequently, the development of suitable analytical methodologies to detect nuts in processed foods is advisable. Real-Time PCR allowed a specific and accurate amplification of allergen sequences. Some food processing methods could induce structural and/or conformational changes in proteins by altering their allergenic capacity, as well as produce the fragmentation and/or degradation of genomic DNA. In this work, we analysed by means of Real-Time PCR, the influence of pressure and thermal processing through Instant Controlled Pressure Drop (DIC) on the detectability of hazelnut, pistachio and cashew allergens. The detection of targets in hazelnut, pistachio and cashew (Cor a 9, Pis v 1 and Ana o 1, respectively) is affected by the treatment to different extents depending on the tree nut. Results are compared to those previously obtained by our group in the analysis of different treatments on the amplificability of the same targets. Reduction in amplificability is similar to that reported for some autoclave conditions. Our assays might allow for the detection of up to 1000 mg/kg of hazelnut, pistachio and cashew flours after being submitted to DIC treatment in food matrices.
Somatic embryogenesis from immature inflorescences of rye
Plant Science, 1990
ABSTRACT Immature inflorescences of four cultivars of rye were cultured on Murashige and Skoog me... more ABSTRACT Immature inflorescences of four cultivars of rye were cultured on Murashige and Skoog medium (MS) with different concentration of 2,4-dichlorophenoxyacetic acid (2,4-D). Embryogenic calluses were obtained in most cases. However the frequency varied in regard to inflorescence length, 2,4-D concentration and donor cultivar. Evidence is provided that the genotypic effect on the in vitro response of an allogamous cultivar in rye is due to two factors: the number of competent plants and the response level of each individual.
Genetic analysis of a polyembryonic mutant in rye
Sexual plant reproduction, Sep 1, 1994
We have obtained one plant regenerated from rye tissue culture which showed a high percentage of ... more We have obtained one plant regenerated from rye tissue culture which showed a high percentage of polyembryonic seeds in its progeny. The mutation inducing the development of extra embryos is also influencing erroneous cell division, mitosis and meiosis. The genetic analysis indicated that the aptitude for polyembryonic seed formation is a heritable trait controlled by a dominant gene. However, for
BioTechniques, Apr 1, 2001
Quantification of DNA
Quantification of DNA is a very important step in many procedures where it is necessary to know t... more Quantification of DNA is a very important step in many procedures where it is necessary to know the amount of DNA that is present when carrying out restriction digests or performing different techniques such as PCR and RAPDs. There are several methods for quantifying DNA, the most widespread being: (i) the comparison of an aliquot of the extracted sample with standard DNAs of known concentration using gel electrophoresis and (ii) spectrophotometric determination. With both methods additional information is gained concerning the quality and purity of the extracted sample obtained. Normally both methods are used, but if the amount of DNA available is very small the gel electrophoresis technique alone may be employed. If there is no limitation on DNA amount, however, spectrophotometric measures should also be taken.
Theoretical and Applied Genetics, Feb 1, 2000
RAPD analysis was performed to assess DNA variation among rye plants regenerated from immature em... more RAPD analysis was performed to assess DNA variation among rye plants regenerated from immature embryos and inflorescences. From the studied plants, 40% showed at least one variation, and the number of mutations per plant was quite high, ranging from 1 up to 12. On some occasions (2.9% of the scored bands) the modified band was observed in only one plant or in several but originated from the same callus (variable band). In other cases (5.25%) the same band varied in several plants obtained from different calli. We call these hypervariable bands and they could vary between plants belonging to different cultivars and/or with different origins, inflorescences or embryos. Thus, they must originate through independent mutational events. We assume that these bands represent hypervariable regions of the rye genome and so detect hot spots of DNA instability. Some of these bands proved to be unique sequences, others were present in a low copy number while the remaining ones were moderately or highly repetitive.
Ecological Intensification Strategies Increase Abundance of Denitrifying Functional Genes in a Greenhouse Agricultural Soil
Foods
Food allergy is a worldwide health problem that concerns all ages from infants to adults [...]
Euphytica, 1996
In the course of experiments performed to obtain haploid wheat plants in which 2,4-dichlorophenox... more In the course of experiments performed to obtain haploid wheat plants in which 2,4-dichlorophenoxyacetic acid (2,4-D) was applied to developing spikes, it was found that three cultivars showed a different ability to produce polyembryos (Thatcher 20 .19%, Chris 7 .06%, Dollar 0%). This behaviour was related to their capacity to form somatic embryos. Diploid immature embryos cultured in vitro after 2,4-D treatment, gave a higher frequency of embryogenic callus in Thatcher and Chris than in Dollar. As the common factor in both experiments was the 2,4-D treatment we propose that the three cultivars showed a differential sensitivity to 2,4-D .
Isolation of plant regulatory sequences by random GUS gene fusion
Critical Reviews in Food Science and Nutrition, 2019
Currently, food allergies are an important health concern worldwide. The presence of undeclared a... more Currently, food allergies are an important health concern worldwide. The presence of undeclared allergenic ingredients or the presence of traces of allergens due to accidental contamination during food processing poses a great health risk to sensitized individuals. Therefore, reliable analytical methods are required to detect and identify allergenic ingredients in food products. Real-time PCR allowed a specific and accurate amplification of allergen sequences. Some processing methods could induce the fragmentation and/or degradation of genomic DNA and some studies have been performed to analyze the effect of processing on the detection of different targets, as thermal treatment, with and without applying pressure. In this review, we give an updated overview of the applications of real-time PCR for the detection of allergens of tree nut in processed food products. The different variables that contribute to the performance of PCR methodology for allergen detection are also review and discussed.
Analysis of T-DNA-mediated translational �-glucuronidase gene fusions
Plant Mol Biol, 1998
Molecular aspect of somaclonal variation in rye
DNA extraction: plant total DNA
A universal genomic DNA microextraction method for PCR, RAPD, restriction and Southern analyses
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Papers by Rosario Linacero