Papers by Manmohan Sharma
Journal of Pharmacognosy and Phytochemistry, 2020
Archives of Agriculture and Environmental Science
The experiment was conducted to investigate the parental diversity along the rice genome and to u... more The experiment was conducted to investigate the parental diversity along the rice genome and to understand and screen out the SSR markers-indicated polymorphism between two indica rice (Oryza sativa L.) cultivars. Namely K343, the most well-liked rice variety in the hill zone of the Jammu Region, and RML22, a rice line created at IRRI, Philippines. The study is to select polymorphic markers (Simple Sequence Repeat- SSR) associated with hill ecologies rice cultivars and additional research projects like gene pyramiding and background selection to recover the recurrent parent genome (RPG) in blast gene introgression in elite lines. 450 SSR markers, evenly distributed throughout the rice genome, were used to assess the parental polymorphism between these genotypes. Of these two cultivars, 51 markers (11.33%) showed polymorphism with bands in different spectrums throughout the genome. The study has been used to Marker Assisted Backcross (MAB) breeding to integrate rice blast resistance ...
Journal of Pharmacognosy and Phytochemistry, 2021
The present study was carried out to analyse the anther culture response of four commercial rice ... more The present study was carried out to analyse the anther culture response of four commercial rice hybrids namely; DRRH3, PRH10, PHB7 and Indira Sona towards haploid induction with respect to cold pre treatment along with effect of growth hormones and amino acids on the callus induction and regeneration. N6 medium augmented with different concentration and combinations of growth hormones and amino acids was used to record the response of genotypes for anther culture, calli regeneration, haploid production and root induction. It was observed that callus induction frequency increased with increase in 2,4-D concentration (1.5 to 2.5 mg l -1 ). In addition, fortification of amino acids like tryptophan (25 mg l -1 ) and cysteine (40 mg l -1 ) showed a synergistic effect on callus induction ability. DRRH3 showed significantly higher scores of androgenic frequencies in comparison to other hybrids. Rooting was effectively induced in liquid half-strength MS medium. It was concluded that callus induction, regeneration, haploid production and androgenesis are genotype specific. However, slight manipulations in cold pretreatment prior to in vitro culturing and media composition can improve the response of genotype towards haploid induction.
The Indian Journal of Agricultural Sciences
Mentha piperita is a completely sterile hybrid due to the fact that male organs of flower fail to... more Mentha piperita is a completely sterile hybrid due to the fact that male organs of flower fail to develop. The stamens remain abnormally short, sometimes degenerate and wither from bud. As a result it is not amenable to improvement by sexual crosses. Ruthless exploitation of peppermint due to growing interest in the commercialization of plant based medicines has lead to drastic decrease of natural resource base which will result in extinction. In order to meet pharmaceutical needs and to provent the plants from extinction, development of rapid large scale propagation systems is a necessity. In vitro multiplication was carried out for mass production of disease free planting material without seasonal constraints ensuring germplasm conservation and sustainable use of the medicinal crop.
International Journal of Agriculture System
Doubled haploid plants are very important for the development of complete homozygous plants from ... more Doubled haploid plants are very important for the development of complete homozygous plants from heterozygous parents in one generation as they possess duplicate copy of haploid chromosome. Haploid production is easily obtained from in vitro anther culture. The present study was undertaken with the objective to develop doubled haploids using anthers for in vitro induction of callus on N6 medium supplemented with various combinations and concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D) (0.5-2.5 mg/L), Kinetin (0.5-1.0 mg/L) and Naphthalene acetic acid (NAA) (2.0 mg/L) as callus induction medium (CIM). The highest callus induction frequency was obtained when N6 medium fortified with 2,4-D (2.5 mg/L), Kinetin (0.5 mg/L) and NAA (2 mg/L) of 10.07 per cent. The induced callus was sub cultured for shoot regeneration on Murashige and Skoog medium (MS) supplemented with growth regulators: Kinetin and NAA (0.5 mg/L each) in combination with BAP (0.0 - 2.5 mg/L). MS medium supplement...
Sustainability
The cold tolerance studies were carried out in a bi-parental F2 population of a cross between tol... more The cold tolerance studies were carried out in a bi-parental F2 population of a cross between tolerant and susceptible parents (SKUA-529 and HEERA, respectively). The purpose was to screen the individuals of a population for primary cold-tolerance-related attributes. The information generated has a direct application and use in identifying cold tolerance quantitative trait loci (QTLs) and further can be used for genotyping with an appropriate marker system. The screening was carried out on F2-derived F3 seeds and F3 plants for seedling and agronomic traits, respectively. Two tests measuring cold tolerance were conducted. In experiment I, seeds were germinated for 28 days at 13 °C and 7 days at 28 °C, and in experiment II, the seeds were germinated for 72 h at 28 °C, 96 h at 13 °C, and once more for 72 h at 28 °C. Coleoptile length, germination percentage, and radical reduction percentages were all measured in experiment I. The radicle and coleoptile regeneration in experiment II wer...
International Journal of Advance Research, Ideas and Innovations in Technology, 2018
Indian mustard is the second most important oilseed crops of India, next to groundnut sharing 27.... more Indian mustard is the second most important oilseed crops of India, next to groundnut sharing 27.8% in India’s oilseed economy. The improved mustard seeds contain 39-44% oil. Oil quality is determined by fatty acid profile, whereas, level of erucic acid predicts the quality of seed oil. Breeding of oilseed has evoked a strong bottleneck selection towards double-low (00) seed quality with zero euroic acid and low seed Glucosinolate content. DNA based molecular markers are important tools in breeding programmes for crop improvement. The main role of these makers to detect the polymorphism. The experimental material comprised 71 genotypes including parents, half diallel crosses and simplified triple test crosses for study. In the molecular characterization of erucic acid and glucosinolate using 4 SSR markers was clustered into three groups. Molecular analysis was done using four markers and a total of 56 amplified bands were obtained, out of which 17 were polymorphic. All the genotypes...
Division of Plant Breeding & Genetics; Division of Plant Pathology; School of Biotechnology; and ... more Division of Plant Breeding & Genetics; Division of Plant Pathology; School of Biotechnology; and Division of Plant Biochemistry and Physiology, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, Chatha, Jammu, Jammu & Kashmir-180 009, India Rice & Wheat Research Station, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya (CSK HPKV), Malan, Kangra, Himachal Pradesh, India
Brazilian Journal of Microbiology, 2021
Télécharger le PDF intégral, notamment pour les sources accessibles sous authentification Veille ... more Télécharger le PDF intégral, notamment pour les sources accessibles sous authentification Veille Bacillus du 25/03/2021 au 01/04/2021 Sommaire > Veille Bacillus > 1. Publications Efficacy of bioinoculants to control of bacterial and fungal diseases of rice (Oryza sativa L.) in northwestern Himalaya Biological control of Fusarium wilt of Ustica landrace lentil Microorganisms as biocontrol agents against bacterial citrus diseases Biological control of Fusarium wilt of sesame by Penicillium bilaiae 47M-1 Biocontrol agent, biofumigation, and grafting with resistant rootstock suppress soil-borne disease and improve yield of tomato in West Virginia Genome Mining, Heterologous Expression, Antibacterial and Antioxidant Activities of Lipoamides and Amicoumacins from Compost-Associated Bacillus subtilis fmb60 Combining potential oomycete and bacterial biocontrol agents as a tool to fight tomato Rhizoctonia root rot Genomic insights into biocontrol potential of edible seaweed-associated Bacillus velezensis MTCC 10456 from Gulf of Mannar > 2. Brevets ANTIFUNGAL LIPOPEPTIDES PRODUCED BY PLANT GROWTH PROMOTING RHIZOBACTERIAL STRAIN BACILLUS AMYLOLIQUEFACIENS RHNK22 (MTCC 25230) AND THEIR APPLICATION AS BIOCONTROL AGENTS VEILLE BACILLUS 1. PUBLICATIONS Efficacy of bioinoculants to control of bacterial and fungal diseases of rice (Oryza sativa L.
International Journal of Current Microbiology and Applied Sciences, 2020
Frontiers in Plant Science, 2019
Modern genome editing (GE) techniques, which include clustered regularly interspaced short palind... more Modern genome editing (GE) techniques, which include clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system, transcription activator-like effector nucleases (TALENs), zinc-finger nucleases (ZFNs) and LAGLIDADG homing endonucleases (meganucleases), have so far been used for engineering disease resistance in crops. The use of GE technologies has grown very rapidly in recent years with numerous examples of targeted mutagenesis in crop plants, including gene knockouts, knockdowns, modifications, and the repression and activation of target genes. CRISPR/Cas9 supersedes all other GE techniques including TALENs and ZFNs for editing genes owing to its unprecedented efficiency, relative simplicity and low risk of off-target effects. Broad-spectrum disease resistance has been engineered in crops by GE of either specific host-susceptibility genes (S gene approach), or cleaving DNA of phytopathogens (bacteria, virus or fungi) to inhibit their proliferation. This review focuses on different GE techniques that can potentially be used to boost molecular immunity and resistance against different phytopathogens in crops, ultimately leading to the development of promising disease-resistant crop varieties.
Electronic Journal of Plant Breeding, 2019
Basmati rice grain quality traits are the parameters which determine the export potential. Among ... more Basmati rice grain quality traits are the parameters which determine the export potential. Among various parameters, amylose content along with grain dimensions are important grain quality traits of Basmati rice. A total of 140 recombinant inbred lines (RILs) developed by crossing Basmati 370 and Pusa Basmati 1 were used in the present study to determine the relationship between amylose content and grain dimensions namely, grain length, breadth and length breadth ratio. Significant variations for grain length and amylose content were observed among the RILs. Grain length ranged from 6.3 mm (RIL 40) to 8.1 mm (RIL 101) whereas the amylose content ranged from low (18.1%) to intermediate (24.5%). Amylose content showed a positive and significant correlation with grain length and grain breadth whereas it was found to be negatively correlated with grain length to grain breadth ratio. This study suggested that for better cooking quality in basmati rice an optimum balance has to be maintained between the grain dimensions, particularly grain length and amylose content.
Natural Resources Conservation and Research, 2019
Climate change is an unequivocal fact and its impacts are already perceptible today. Climate chan... more Climate change is an unequivocal fact and its impacts are already perceptible today. Climate change will profoundly alter the present conditions of agriculture in almost all countries with variable severity from region to region. On one hand unpredicted changes in climatic parameters are likely to threaten the production and productivity of economically important plant species particularly that of food crops, at the same time agriculture productivity requires significant increase to meet the expected growth in demand for food by the ever increasing world population. Conservation and sustainable use of diversity present in plant genetic resources and traditional knowledge of germplasm within and among plant species represent economic, scientific and societal value which has tendency to solve the food security problems erupt due to expanding global population. An advance made in biotechnology field such as in vitro culture technology, cryopreservation and molecular markers technology ...
Journal of Pharmacognosy and Phytochemistry, 2018
In the present study, F2 segregating population was developed from the crosses between the parent... more In the present study, F2 segregating population was developed from the crosses between the parental lines K343 and DHMAS rice during Kharif 2016 and evaluated for statistical description, genetic variability components i.e. GCV & PCV, heritability (broad sense) and genetic advance of various agronomical traits. The experiment was conducted using augmented design-I, analysis of variance (ANOVA) revealed statistically significant differences (p<0.05) indicating the existence of genetic variability amongst the population. The mean value and range in days to maturity, duration of grain filling and spikelet fertility % age was greater than that of other traits. The values recorded for genotypic variance was less than those of the phenotypic variance. In the present study spikelet density (7.24) and yield per plant (7.02) showed the highest GCV values whereas days to maturity showed the lowest GCV value (2.61). The low GCV and PCV values, indicates that trait expression was less influe...
International Journal of Plant & Soil Science, 2021
Assessment of genetic diversity was made for sixteen yields and yield contributing traits in twen... more Assessment of genetic diversity was made for sixteen yields and yield contributing traits in twenty-one tomato genotypes using D2 statistics. These genotypes were grouped into five clusters by the use of the Tocher method. Cluster I was the most significant, comprising 13 genotypes (Pusa Ruby, CLN2123A1 Red, PKM-1, IIHR-2042, Marglobe, EC 163605, Hawaii 7998, Palam Pink, EC 521038, Punjab Rakthak, EC 163383, Arka Abha and IIVR BT-10) followed by Cluster II with 5 genotypes (EC 163611, Punjab Sartaj, EC 160885, Punjab Barkha Bahar and Roma). Other Clusters (II, IV and V) were solitary ones (EC 249515, Kashi Chayan, DVRT-2). Cluster II recorded a maximum intra cluster distance of 3521.73 followed by cluster I (2796.45), whereas, cluster II, IV and V had zero intra cluster distance. The inter cluster D2 value also ranged widely, with a minimum value of 4082.34 between cluster I and IV, indicating a close relationship among the genotypes included. The maximum inter cluster D2 value (2...
International Journal of Current Microbiology and Applied Sciences, 2020
International Journal of Current Microbiology and Applied Sciences, 2019
Source Variation in Important Seed and Nursery Characteristics of Grewia optiva D. and Celtis australis L
Journal of Research Skuast J, 2011
Anther culture is a biotechnology technique that can be used for production of pure lines in many... more Anther culture is a biotechnology technique that can be used for production of pure lines in many species. The aim of this experiment is to study comparative response of anther culture on different genotypes of Brassica. In order to access the effect of anther culture response in different genotypes of Brassica, three varieties of B. napus [GSL1, DGS1 and RSPN25) and three varieties of Brassica juncea (RSPR01, Varuna and Kranti) were evaluated. The best response of callus induction was observed in MS medium supplemented with 2, 4-D (1.0 mg l -1 ) and NAA (0.5 mg l -1 ). Enhancement of regeneration of plantlets was achieved by sub culturing embryogenenic callus on media containing different concentrations of cytokinins [BAP and Kinetin] and the best response of embryogeniccalli was got with kinetin [2.0 mg l 1 ]. The best rooting was achieved with 0.4 mg/l IBA. After 5 days of rooting root samples were taken for confirmation of haploid nature of the plants. Haploid frequency was more...
International Journal of Current Microbiology and Applied Sciences
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Papers by Manmohan Sharma