Papers by Saeed Ziaei-nejad
Journal of Aquaculture Development, May 10, 2016

Effects of Supplemental Feeding of Common Carp (Cyprinus carpio) with Iron Nanoparticles and Probiotic Lactobacillus on Blood Biochemical Factors
Biology bulletin of the Russian Academy of Sciences, Mar 1, 2021
Abstract The purpose of this study was to investigate the effect of commercial Lactobacillus prob... more Abstract The purpose of this study was to investigate the effect of commercial Lactobacillus probiotic and iron nanoparticles on some blood biochemical parameters of common carp ( Cyprinus carpio ). Common carp (mean weight 50 g) was fed in six treatments with 0.25 and 0.50 mg/g iron oxide nanoparticles and 10 8 CFU/g commercial Lactobacillus probiotic either together or separately for 56 days. After the rearing period, blood biochemical parameters including total plasma protein, albumin, globulin, creatinine, triglyceride and cholesterol and ALP, ALT, AST and LDH enzymes were measured. Total protein and globulin levels showed a significant increase in probiotic alone and probiotic plus 0.50 mg iron nanoparticles treatments ( p ≤ 0.05). Creatinine, albumin, liver enzymes, serum lipids did not show any significant differences between treatments ( p ≥ 0.05), but the values of these parameters showed no negative effects in different treatments. Based on the findings of this study, it can be concluded that using iron nanoparticles and Lactobacillus probiotic can improve some of the biochemical factors in carp.
Effects of processing on the proximate composition and energetic values in two fish species from Iran southern waters
Ukrainian Food Journal, 2015

Effects of diets containing grape pomace on the growth, nutrition indices, and the quality traits of common carp (<i>Cyprinus carpio</i>)
Food Science and Nutrition, Aug 10, 2023
The fish diet is one of the essential factors in the development of aquaculture. The purpose of p... more The fish diet is one of the essential factors in the development of aquaculture. The purpose of present study was to evaluate using grape pomace as a main feed ingredient on growth performance, body chemical composition, survival rate, and morphological indices of the carp (Cyprinus carpio). 200 fish with an average weight of 7 ± 0.4 g were randomly distributed in four tanks for total of 56 days. The fish were fed with a diet containing 5%, 10%, and 15% grape pomace in different feeding groups designated as G1, G2, and G3. The average daily weight gain (g), weights gain (g), and specific growth rate (%) were significantly higher (p &lt; .05) in G3 as compared to G1 and G2 groups. The lowest feed conversion ratio was recorded in G3 group. The morphological indices, condition factors, viscerosomatic index, and hepatosomatic index were significantly higher in G3 group as compared to other treatments. The protein, fat, moisture, and ash contents in the C. carpio fillets were significantly influenced by feeding rate. The results showed that grape pomace had a positive effect on growth, survival, and nutritional indices in the carp fish. According to the obtained results, grape pomace (150 g/kg food) can be considered as the diet component for the carp fish.

Effect of <i>Beta vulgaris</i> L. as feed ingredient on muscle growth, nutritional factors, and quality of common carp, <i>Cyprinus carpio</i>
Food Science and Nutrition, Jun 9, 2023
The purpose of this study was to evaluate the utilization of Beta vulgaris powder as feed ingredi... more The purpose of this study was to evaluate the utilization of Beta vulgaris powder as feed ingredient in the diet of Cyprinus carpio for a period of 56 days. Common carp fry with an average weight of 20 ± 0.2 g were fed using the diet containing B. vulgaris leaves with different concentrations. Fry of Cyprinus carpio were equally distributed in four feeding groups having three replicates each. The study was conducted indoors, in FRP tanks, and aeration was provided to individual rearing units, and it was a flow‐through system. The basal diet was replaced at 0.5%, 1%, and 2% with B. vulgaris powder. The basal diet without B. vulgaris powder (0%) served as control (T1). Significantly higher feed conversion ratio, protein efficiency ratio, and fat and protein indices were recorded in fish fed with B. vulgaris powder in treatments. Different feeding groups showed greater acceptability of B. vulgaris powder mixed diet without any adverse behavioral response. The protein and fat average percentages were 43.32 and 10.79 g, when fish reached commercial weight (48.02, 11.85 g) after 56 days for treatment 2%. After fish feeding with the B. vulgaris, for treatment 2% lower moisture than the control was recorded. The carp fish diet containing 1% B. vulgaris leaf powder caused a significant decrease in the fish fat content. It can be concluded that the diet containing 2% B. vulgaris leaf powder in the common carp led to better growth performance. The presence of B. vulgaris leaves in the fish diet increased the fillet protein and ash content.

Effects of diets containing grape pomace on the growth, nutrition indices, and the quality traits of common carp (Cyprinus carpio)
Food Science & Nutrition
The fish diet is one of the essential factors in the development of aquaculture. The purpose of p... more The fish diet is one of the essential factors in the development of aquaculture. The purpose of present study was to evaluate using grape pomace as a main feed ingredient on growth performance, body chemical composition, survival rate, and morphological indices of the carp (Cyprinus carpio). 200 fish with an average weight of 7 ± 0.4 g were randomly distributed in four tanks for total of 56 days. The fish were fed with a diet containing 5%, 10%, and 15% grape pomace in different feeding groups designated as G1, G2, and G3. The average daily weight gain (g), weights gain (g), and specific growth rate (%) were significantly higher (p < .05) in G3 as compared to G1 and G2 groups. The lowest feed conversion ratio was recorded in G3 group. The morphological indices, condition factors, viscerosomatic index, and hepatosomatic index were significantly higher in G3 group as compared to other treatments. The protein, fat, moisture, and ash contents in the C. carpio fillets were significant...

Effect of Beta vulgaris L. as feed ingredient on muscle growth, nutritional factors, and quality of common carp, Cyprinus carpio
Food Science & Nutrition
The purpose of this study was to evaluate the utilization of Beta vulgaris powder as feed ingredi... more The purpose of this study was to evaluate the utilization of Beta vulgaris powder as feed ingredient in the diet of Cyprinus carpio for a period of 56 days. Common carp fry with an average weight of 20 ± 0.2 g were fed using the diet containing B. vulgaris leaves with different concentrations. Fry of Cyprinus carpio were equally distributed in four feeding groups having three replicates each. The study was conducted indoors, in FRP tanks, and aeration was provided to individual rearing units, and it was a flow‐through system. The basal diet was replaced at 0.5%, 1%, and 2% with B. vulgaris powder. The basal diet without B. vulgaris powder (0%) served as control (T1). Significantly higher feed conversion ratio, protein efficiency ratio, and fat and protein indices were recorded in fish fed with B. vulgaris powder in treatments. Different feeding groups showed greater acceptability of B. vulgaris powder mixed diet without any adverse behavioral response. The protein and fat average pe...

This study was carried out in order to assess the effect of inulin and Bacillus on nutrition and ... more This study was carried out in order to assess the effect of inulin and Bacillus on nutrition and growth indices of rainbow trout (Oncorhynchus mykiss). For this purpose, 525 of fish rainbow trout with an average weight of 56.84g ± 4.32 were purchased from fish breeding farm and transferred to the aquaculture department of Shiraz University. After initial adaptation and one week of quarantine, 25 fish were stored in each 500 L polyethylene tank. The duration of the experiment was 60 days. Triplicates were used for each treatment experimental groups included a control (C) without any supplement, treatment (A) with a level of 0.5% inulin, treatment (B) with a level of 1.5% inulin, treatment (D) with a concentration of 103 CFU/g Bacillus, treatment (E) with 106 CFU/g Bacillus, treatment (F) with 103 CFU/g Bacillus and 0.5% inulin and treatment (H) with 106 CFU/g Bacillus and 1.5% inulin. The ratio of feed conversion on 15th, 30th and 60th days in F treatment showed a significant increas...
Study on the hematological toxicity of Cyprinus carpio exposed to water-soluble fraction of crude oil and TiO2 nanoparticles in the dark and ultraviolet
Chemosphere

Background The optimizing nutritional conditions are effective in the quantitative and qualitativ... more Background The optimizing nutritional conditions are effective in the quantitative and qualitative position of carp production. The use of plant material in the fish diet to reduce production costs and increase growth rate. Objectives The aim of present research was to investigate the effect of different levels of powder Beta vulgaris was preformatted on some indices of nutrition and the fillet analysis of common carp (Cyprinus carpio). Methods The 360 fish with a weight average of 20 g and a standard length of 9 cm were selected and randomly divided into 12 tanks. The experiment consisted of 4 treatments and 3 triplicates. The fishes were fed to diet containing 0.5%, 1% and 2% of Beta vulgaris powder for 6 weeks. The nutrition indices and chemical composition of the fish fillet were investigated. Results The adding Beta vulgaris leaves powder to the fish diet had a positive effect on the nutrition indices. Feed conversion ratio was increased by adding Beta vulgaris to diet, and the...

Evaluation of nutritional quality and fatty acid profile of carp (Cyprinus carpio) fed with diet based on oil sources
Current Nutrition & Food Science
Background: The Cyprinus carpio as commercial fish in Iran contains nutritive components and play... more Background: The Cyprinus carpio as commercial fish in Iran contains nutritive components and plays an important role in human health. The present study research evaluated the nutritive quality of carp including proximate and fatty acid compositions. Objective: The aim of the present study was to investigate the effects of diet based on oil sources on the fatty acids composition of fish fillets of Cyprinus carpio. Methods: The carp (Cyprinus carpio) were fed with four diets based on soybean oil (SO), palm oil (PO), fish oil (FO), and (soybean and palm) oil (SPO). Results: The quality of fatty acids in the body of fish fed with SO and PO diets was found significantly better (p <0.05) than those fish fed with FO diet. Total SFA (Saturated fatty acids) was highest significant in the fillet fat of the carp fish fed with PO diet, but there were no significant differences between the MUFA (Mono unsaturated fatty acids), PUFA (Poly unsaturated fatty acids), HUFA (High unsaturated fatty a...

تجمع بافتیکلوئید نانو ذرات نقره در بافتهای آبشش و عضله ساقه دمی در ماهی کپور معمولی (Cyprinus carpio)
با توجه به روند روز افزون مصرف نانو ذرات و رهایش آن در بوم سازگان آبی، بیم خطرات احتمالی برای محی... more با توجه به روند روز افزون مصرف نانو ذرات و رهایش آن در بوم سازگان آبی، بیم خطرات احتمالی برای محیط زیست و پیرو آن، برای انسان می¬رود. هدف از پژوهش حاضر، بررسی تجمع بافتی نانو ذرات نقره و تأثیر آن بر بازماندگی در ماهی کپور معمولی (Cyprinus carpio) است. به این منظور، طی یک دوره 60 روزه، ماهیان در معرض غلظت¬های متفاوت نانو ذرات نقره (0، 025/0، 05/0، 1/0 و 2/0 ppm ) قرار داده شد. طول دوره¬ی آداپتاسیون 2 هفته در نظر گرفته شد. در هر تانک 25 قطعه ماهی کپور معمولی قرار داده و آزمایش با 5 تیمار و 3 تکرار انجام شد. نتایج حاصل از تجمع نانو ذرات نقره در بافت آبشش و عضله ساقه دمی ماهی کپور معمولی، در پایان دوره تیمار بندی، اختلاف معناداری را بین تجمع بافتی در تیمارها با غلظت¬های متفاوت، نشان داد. با افزایش غلظت نانو ذرات نقره مورد استفاده، تجمع بافتی در بافت¬های مختلف افزایش یافته (05/0&lt;p)، با این حال غلظت 2/0 ppm نانو نقره دریافتی، بیشترین میزان تجمع نانو نقره را در همه¬ی بافت¬ها نشان داد. کمترین تجمع بافتی در آبشش و بیشترین تجمع در بافت عضله ساقه دمی مشاهده شد. نتایج درصد بازماندگی در پایان دوره 60 روزه¬ی تیمار بندی با افزایش غلظت نانو ذرات، افزایش کمی را نشان داده، با این حال، هیچ اختلاف معناداری بین درصد بازماندگی در دوز¬های مختلف رویارویی ماهیان با نانو ذرات نقره وجود ندارد (05/0&lt;P). به نظر می¬رسد که حضور نانو ذرات نقره چه به عنوان آلاینده محیطی و چه به هدف استفاده از فنانوری نانو باعث تجمع بافتی به خصوص در بافت¬های داخلی ماهی و تأثیر منفی هر چند اندک بر بازماندگی آبزی دارد. با توجه به این که ماهیان به خصوص ماهی کپور معمولی در زنجیره غذایی انسان جایگاه مشخصی دارد، بنابر این جلوگیري از ورود این مواد جدید به چرخه غذایی یا محیط زیستمندان ساکن بوم سازگان¬هاي آبی بسیار مهم می باشد. از طرف دیگر، استفاده از فناوری نانو ذرات نقره به عنوان روش درمانی جهت مبارزه با بیماری های آبزیان توصیه نمی شود.

Food Science and Technology, 2018
Because of fishes Sparidentex hasta and Pampus argenteus in the southern of Iran are consumed abu... more Because of fishes Sparidentex hasta and Pampus argenteus in the southern of Iran are consumed abundant in a particular season and it should be frozen for consumption throughout the year. Therefore, this research was carried out to investigate the effects of freezing on some of the physicochemical properties of fillets the fishes. Factors such as fat with chloroform-methanol method, amount of TBA (Thiobarbituric acid) in fish muscle accordance method of Pearson, pH using a pH meter, FFA (Free fatty acids) with titration in the presence of phenolphthalein was determined based on the percentage of oleic acid, peroxide value according to AOAC (Association of Official Analytical Chemists), in fresh samples at time zero and after different periods of freezing were tested respectively. Result showd that TBA (Thiobarbituric acid) content in fish fillet, found Pampus argenteus and Sparidentex hasta 0.65 and 0.53 respectively. The results showed that the highest percentage of fat found for Pa...
Turkish Journal of Agriculture - Food Science and Technology, 2015
Fish Platycephalus indicus usually are consumed by southern people in Iran. The present study ass... more Fish Platycephalus indicus usually are consumed by southern people in Iran. The present study assessed the effect of processing on proximate compositions in the fillets of P.indicus. The fish samples were prepared by boiling, baking and frying, while proximate analysis was done by standard methods. Boiling processing method significantly reduced ash content in the fillet whereas fat content was significantly increased in frying. Baking method recorded highest ash content of 10.64%. The highest protein concentration was obtained for boiled fillet (82.73%). Lipid content was recorded highest in fried fillet (17.27%). P. indicus was, rich in fat, protein, and ash, thus its consumption should be encouraged.
International Journal of Agricultural Research, Innovation and Technology, 2016
The chemical composition of four edible plant foods species, three fish species and one prawn wer... more The chemical composition of four edible plant foods species, three fish species and one prawn were analyzed in Food

Czech Journal of Animal Science, 2016
Effects of different levels of Biomin ® IMBO synbiotic, including Enterococcus faecium (as probio... more Effects of different levels of Biomin ® IMBO synbiotic, including Enterococcus faecium (as probiotic), and fructooligosaccharides (as prebiotic) on survival, growth performance, and digestive enzyme activities of common carp fingerlings (Cyprinus carpio) were evaluated. The experiment was carried out in four treatments (each with 3 replicates), including T1 = control with non-synbiotic diet, T2 = 0.5 g/kg synbiotic diet, T3 = 1 g/kg synbiotic diet, and T4 = 1.5 g/kg synbiotic diet. In total 300 fish with an average weight of 10 ± 1 g were distributed in 12 tanks (25 animals per 300 l) and were fed experimental diets over a period of 60 days. The results showed that synbiotic could significantly enhance growth parameters (weight gain, length gain, specific growth rate, percentage weight gain) (P < 0.05), but did not exhibit any effect on survival rate (P > 0.05) compared with the control. An assay of the digestive enzyme activities demonstrated that the trypsin and chymotrypsin activities of synbiotic groups were considerably increased than those in the control (P < 0.05), but there was no significant difference in the levels of α-amylase, lipase, or alkaline phosphatase (P > 0.05). This study indicated that different levels of synbiotic have the capability to enhance probiotic substitution, to improve digestive enzyme activity which leads to digestive system efficiency, and finally to increase growth. It seems that the studied synbiotic could serve as a good diet supplement for common carp cultures.
CULTURE AND BREEDING OF FRESH WATER PRAWN Macrobrachium rosenbergii AS AN EXOTIC SPECIES IN IRAN, PRESENT STATUS AND FUTURE PERSPECTIVE

AACL Bioflux
Aquaculture industry especially shrimp farming in Iran and other parts of the world has been faci... more Aquaculture industry especially shrimp farming in Iran and other parts of the world has been facing various disease that among various agents, pathogenic bacteria has emerged as important because it causes large economic losses to the shrimp farming industry. This study investigated the antagonistic properties of copper nanoparticles (34 mg/L), probiotic Bacillus subtilis (108 CFU/mL), and combination of these two against with pathogenic bacteria, luminescent Vibrio harveyi in vitro condition in order to investigate possible use of probiotic B. subtilis and copper nanoparticles in the diet for growth and immunity enhancement in Litopenaeus vannamei. This study reports that in Well diffusion test and Disc diffusion test against pathogenic bacteria luminescent V. harveyi maximum inhibition showed by combination of suspension of probiotic B. subtilis and copper nanoparticles the amount of (W = 22.54±1.72 and D = 16.37±2.9) whereas minimum inhibition was copper nanoparticles the amount ...

International Journal of Agricultural Research, Innovation and Technology, 2015
This research work was directed on the study of the proximate chemical composition to compare the... more This research work was directed on the study of the proximate chemical composition to compare the effect of the brining constant concentration and different times on the nutritive value of fish. The proximate composition of treated Chirocentrus nudus (protein, fat, ash and energetic value) for 8% salt and 18 h brining was 61.2%, 15.2%, 23.2% and 381.60 kcal/100g respectively. Fat and ash contents in brined fillets of Nemipterus japonicus and Saurida tumbil were found lower and more than C. nudus respectively. As for the proximate composition of C. nudus, it is clearly observed that all the studied parameters are significantly different in fresh and treated samples. Variations appeared in nutrients was due to the interaction of the salting treatment. It is concluded that fish treated C. nudus was best option.

Aquaculture, 2006
This study examined the effects of a commercial Bacillus probiotic on the digestive enzyme activi... more This study examined the effects of a commercial Bacillus probiotic on the digestive enzyme activity, survival and growth of Fenneropenaeus indicus at various ontogenetic stages in three separate experiments: (1) Nauplius 1-2 to Zoea 3 , which were exposed to probiotic added directly to the water; (2) Mysis 1 to PL 14 in tanks, which were exposed to the probiotic either through adding it directly to the water or by feeding shrimp with probiotic-enriched Artemia; (3) postlarval shrimp reared in earthen ponds during the farming stages (PL 30 to PL 120), which were exposed to probiotic added to the water. The counts of Bacillus bacteria in the digestive tract in all treatments were significantly (P b 0.05) higher than in controls (no Bacillus bacteria were detected in any controls), although total bacterial counts were not significantly different among treatments and controls. Colonization rates of shrimp digestive tracts by Bacillus bacteria were very low in all treatments in earthen ponds. In most treatments, the specific activities of amylase, total protease, and lipase were significantly higher (P b 0.05) in shrimp to which probiotic had been administered, and shrimp that had received probiotic exhibited significant (P b 0.05) increases in both survival (11-17% higher) and wet weight (8-22% higher) as compared to controls. Shrimp fed probiotic-enriched Artemia had significantly (P b 0.05) higher Bacillus counts than did shrimp administered probiotic in the water, but growth and survival were not significantly different between the two modes of administration. Where probiotic was administered during both the hatchery stages (Nauplius 1-2 through PL 30) and the farming stages, the feed conversion ratio, specific growth rate, and final production were slightly, but significantly (P b 0.05), higher in shrimp receiving the probiotic than in control shrimp which had received no probiotic. Because these improvements in growth parameters in postlarval shrimp were significant only in shrimp
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Papers by Saeed Ziaei-nejad