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. 2000 Feb;129(3):416-9.
doi: 10.1038/sj.bjp.0703068.

Urothelium-derived inhibitory factor(s) influences on detrusor muscle contractility in vitro

M H Hawthorn  1 , C R Chapple, M Cock, R Chess-Williams
Affiliations

Urothelium-derived inhibitory factor(s) influences on detrusor muscle contractility in vitro

M H Hawthorn et al. Br J Pharmacol. 2000 Feb.

Abstract

The function of the bladder urothelium in modulating contractile responses of the underlying detrusor smooth muscle to muscarinic stimulation has been examined in the pig bladder. Saturation curves for [3H]-QNB binding demonstrated a greater muscarinic receptor density in the urothelium than in the detrusor smooth muscle. The presence of an intact urothelium on isolated bladder strips inhibited contractions induced by carbachol but not KCl. Contractions of a urothelium-denuded muscle strip were inhibited in the presence of a second bladder strip with an intact urothelium, but not if the second strip was denuded. The urothelium-induced inhibition of contractions was not prevented in the presence of L-NOARG, methylene blue, indomethacin, propranolol, suramin, TEA or apamin. The data suggest the presence of a diffusable, urothelium-derived inhibitory factor, which could not be identified but appears to be neither nitric oxide, a cyclo-oxygenase product, a catecholamine, adenosine, GABA nor an EDHF sensitive to apamin.

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Figures

Figure 1
Figure 1
Saturation curve and Scatchard plot for the specific binding of [3H]-QNB to membranes prepared from pig urothelium.
Figure 2
Figure 2
Responses to carbachol of detrusor strips with an intact urothelium or with the urothelium removed. Responses are plotted as a percentage of the maximum response of the denuded tissue. *P<0.001 vs responses of urothelium-denuded tissues.
Figure 3
Figure 3
Responses to carbachol of urothelium-denuded detrusor muscle in the presence of a second bladder strip with or without a urothelium. Responses are plotted as a percentage of the maximum response of the denuded tissue. All responses were depressed in the presence of the urothelium (P<0.001 for all points). Responses in the presence of the denuded muscle strips were significantly greater than in the presence of an intact urothelium (P<0.05 for all points) and were not significantly different to controls (except where indicated, *P<0.05).
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