Shravan Paswan

Hydro alcoholic extract of Holarrhena antidysenterica L. induced toxicity research in experimental animals

Routledge eBooks, May 18, 2023

India’s COVID-19: Diverse Population DemandsDiverse Requisites

EC Pulmonology and Respiratory Medicine, May 25, 2021

Wound Healing Activity of Ethanolic Extract of Selaginella Bryopteris on Rats

Pharmacognosy Journal, Sep 7, 2019

The objective of the present study was to determine wound healing activity of ethanolic extract o... more The objective of the present study was to determine wound healing activity of ethanolic extract of Selaginella bryopteris on rats. The whole plant of S. bryopteris Linn. was collected from Andhra Pradesh, India and extraction was done using ethanol. GC-MS analysis was performed to determine active metabolites present in the extract followed by determination of total phenolic and flavonoid contents. In vivo wound healing activity of ethanolic extract was evaluated using excision wound model. The extract was applied topically on animals by preparing ointment in two concentrations (5% and 10%) where soframycin (10%) was taken as positive control. Antioxidant activity of S. bryopteris extract was observed by measuring oxidative enzymatic levels i.e. Superoxide dismutase (SOD), Catalase (CAT), reduced Glutathione (GSH) and lipid peroxidation (LPO) in animal tissues. Histopathological studies of excised skin were carried out after the experimental period. The contraction rate of the wound was higher and dose-dependent in rats treated with 5% and 10 % ointment of extract in comparison to untreated control group. The drug treated groups showed recovery phase and the percentage of healing was more in 10% at the end of experimental period. Results exhibited sufficient insights on the healing process with normal recovery stages and restored oxidative enzymatic levels. Histopathological findings provided additional positive results; the dermis with proliferating capillaries and skeletal muscle were replaced by cellular fibrous tissue and collagen fibers. Overall, the results showed that ethanolic extract of S.bryopteris was an interesting traditional agent that possess significant wound healing activity.

Download

Phytochemical investigation, antioxidant and wound healing activities of Citrullus colocynthis (bitter apple)

Asian pacific Journal of Tropical Biomedicine, 2018

Biodegradable PEG-PCL Nanoparticles for Co-delivery of MUC1 Inhibitor and Doxorubicin for the Confinement of Triple-Negative Breast Cancer

Journal of Polymers and The Environment, Nov 11, 2022

Combating triple-negative breast cancer (TNBC) is still a problem, despite the development of num... more Combating triple-negative breast cancer (TNBC) is still a problem, despite the development of numerous drug delivery approaches. Mucin1 (MUC1), a glycoprotein linked to chemo-resistance and progressive malignancy, is unregulated in TNBC. GO-201, a MUC1 peptide inhibitor that impairs MUC1 activity, promotes necrotic cell death by binding to the MUC1-C unit. The current study deals with the synthesis and development of a novel nano-formulation (DM-PEG-PCL NPs) comprising of polyethylene glycol-polycaprolactone (PEG-PCL) polymer loaded with MUC1 inhibitor and an effective anticancer drug, doxorubicin (DOX). The DOX and MUC1 loaded nanoparticles were fully characterized, and their different physicochemical properties, viz. size, shape, surface charge, entrapment efficiencies, release behavior, etc., were determined. With IC 50 values of 5.8 and 2.4 nm on breast cancer cell lines, accordingly, and a combination index (CI) of < 1.0, DM-PEG-PCL NPs displayed enhanced toxicity towards breast cancer cells (MCF-7 and MDA-MB-231) than DOX-PEG-PCL and MUC1i-PEG-PCL nanoparticles. Fluorescence microscopy analysis revealed DOX localization in the nucleus and MUC1 inhibitor in the mitochondria. Further, DM-PEG-PCL NPs treated breast cancer cells showed increased mitochondrial damage with enhancement in caspase-3 expression and reduction in Bcl-2 expression.In vivo evaluation using Ehrlich Ascites Carcinoma bearing mice explicitly stated that DM-PEG-PCL NPs therapy minimized tumor growth relative to control treatment. Further, acute toxicity studies did not reveal any adverse effects on organs and their functions, as no mortalities were observed. The current research reports for the first time the synergistic approach of combination entrapment of a clinical chemotherapeutic (DOX) and an anticancer peptide (MUC1 inhibitor) encased in a diblock PEG-PCL copolymer. Incorporating both DOX and MUC1 inhibitors in PEG-PCL NPs in the designed nanoformulation has provided chances and insights for treating triple-negative breast tumors. Our controlled delivery technology is biodegradable, non-toxic, and anti-multidrug-resistant. In addition, this tailored smart nanoformulation has been particularly effective in the therapy of triple-negative breast cancer.

Download

Assessment of Toxicity and Wound Healing Activity of <i>Selaginella Bryopteris</i> Extract

Drug and Chemical Toxicology, May 29, 2022

Phytochemical Investigation and HPTLC Fingerprinting Analysis of<i>Selaginella bryopteris</i>Ethanolic Plant Extract for Analgesic and Anti-inflammatory Activities in Animals

Journal of biologically active products from nature, Jul 4, 2021

Abstract The present study was performed to determine analgesia and anti-inflammatory activities ... more Abstract The present study was performed to determine analgesia and anti-inflammatory activities of whole plant extract of Selaginella bryopteris on animals. The extract of S. bryopteris whole plant was performed using ethanol (70%) as a solvent. The phytochemical investigation of plant extract was done to evaluated secondary metabolites present in the plant. It was further characterized using HPTLC technique to identify active constituents present in the S. bryopteris extract. The efficacy of plant extract was evaluated using the hot plate method and tail flick method against pain and carrageenan-induced paw oedema model against inflammation. Results exhibited that S. bryopteris extract possesses alkaloids, glycosides, tannins, saponins, flavonoids, carbohydrate, protein and amino acids, steroids, vitamins, gums and mucilages. HPTLC fingerprinting analysis revealed the presence of gallic acid and rutin as active constituents present in the plant extract. S. bryopteris extract showed significant analgesic and anti-inflammatory activities. Thus, it can be used as a safer alternative drug against pain and inflammation in comparison to existing non-steroidal anti-inflammatory drugs (NSAIDs).

The International Natural Product Sciences Taskforce (INPST) and the power of Twitter networking exemplified through #INPST hashtag analysis

Phytomedicine

Background: The development of digital technologies and the evolution of open innovation approach... more Background: The development of digital technologies and the evolution of open innovation approaches have enabled the creation of diverse virtual organizations and enterprises coordinating their activities primarily online. The open innovation platform titled "International Natural Product Sciences Taskforce" (INPST) was established in 2018, to bring together in collaborative environment individuals and organizations interested in natural product scientific research, and to empower their interactions by using digital communication tools. Methods: In this work, we present a general overview of INPST activities and showcase the specific use of Twitter as a powerful networking tool that was used to host a one-week "2021 INPST Twitter Networking Event" (spanning from 31st May 2021 to 6th June 2021) based on the application of the Twitter hashtag #INPST. Results and Conclusion: The use of this hashtag during the networking event period was analyzed with Symplur Signals (https://www.symplur.com/), revealing a total of 6,036 tweets, shared by 686 users, which generated a total of 65,004,773 impressions (views of the respective tweets). This networking event's achieved high visibility and participation rate showcases a convincing example of how this social media platform can be used as a highly effective tool to host virtual Twitter-based international biomedical research events.

Download

Pain Allaying Epalrestat-Loaded Lipid Nanoformulation for the Diabetic Neuropathic Pain Interventions: Design, Development, and Animal Study

Current Drug Metabolism

Background: Diabetic peripheral neuropathy is the most common complication of diabetes mellitus. ... more Background: Diabetic peripheral neuropathy is the most common complication of diabetes mellitus. Epalrestat, an aldose reductase inhibitor, has been approved for clinical therapy for diabetic peripheral neuropathic pain. In the present study, solid lipid-based nanoparticles are used for oral administration of epalrestat (E-SLN) and evaluated against diabetic neuropathic pain in a rat model. Method: Experimental diabetes in rats was induced by a single dose of streptozotocin (STZ) administration. The therapeutic efficiency of Epalrestat nanoparticles (0.25, 0.50, 1, and 5 mg/kg) in diabetic rats was studied. STZ induced diabetic rats were treated with different doses of E-SLN for 8 weeks. The nanoparticles were orally administered at a single dose in rats, and the various parameters related to peripheral neuropathy were evaluated and compared with the bare drug. The blood glucose level was estimated by standard glucometer, HbA1c, triglycerides, total cholesterol and liver function te...

Chronic Pneumonia

Extract of Moringa concanensis Nimmo Leaves Ameliorates Hyperglycemia and Oxidative Stress, and Improves β-cell Function in Alloxan Monohydrate Induced Diabetic Rats

Current Bioactive Compounds, 2021

Background: The ethanomedicinal importance of Moringa concanensis Nimmo plant is reflected in the... more Background: The ethanomedicinal importance of Moringa concanensis Nimmo plant is reflected in the Ayurvedic and traditional system of medicine. It has vast usage in Ayurvedic preparation and diabetes management. Aims of the Study: The research was centered on bringing out the hyperglycemic capabilities of Moringa concanensis Nimmo leaves, and their ethanolic extract (PE) on alloxan monohydrate (AXM) induced diabetic rat model. Materials and Methods: Wistar rats were made diabetic by AXM and treated with PE (200 mg/kg body weight) and glibenclamide as the standard drug. All essential parameters like fasting blood glucose (FBS), post-prandial blood glucose (PPBS), AST, ALT, ALP, ACP, LDH, and oxidative stress markers were measured. Also, to observe β-cells structures, histology of the pancreas was studied. Results: The non-toxicity of PE dose was confirmed by an acute toxicity study. This study model helped determine the anti-hyperglycemic effects of PE by decreasing FBS and PPBS leve...

Current Understanding to Accelerate Wound Healing: Mechanism and Clinical Importance

Recent Advances in Wound Healing, 2022

Wound mending is a complex organic cycle that brings about the reclamation of tissue honesty. Phy... more Wound mending is a complex organic cycle that brings about the reclamation of tissue honesty. Physiologically, it very well may be separated into four particular periods of hemostasis, inflammation, proliferation, and tissue remodeling (redesigning). This chapter portrays the cellular premise of wound mending and extracellular flagging cycles, which is responsible to control them. The capacity of fibroblasts, neutrophils, platelets, and macrophages is contemplated exhaustively. The idea of mending by essential and optional expectation is talked about. Numerous components are known to unfavorably influence mending including undernourishment, hypoxia, immunosuppression, ongoing sickness, and medical procedure. It is fundamental that specialists comprehend the key physiological cycles associated with mending to limit patient illness from postponed recuperating.

(whole plant) Linn. Against Isoniazid & Rifampicin Induced Hepatic Toxicity in Wistar rats

Introduction and objective: The scientific assessment of medicinal plants used in the preparation... more Introduction and objective: The scientific assessment of medicinal plants used in the preparation of folk remedies has contributed modern medicine with effective pharmaceuticals for the treatment of diseases. Objective: The 50 % ethonolic extract of solanum xanthocarpum (Solanaceae) whole plant was explored for its hepatoprotective and antioxidant effects on RIF+INH (50 mg/kg) induced acute liver damage in Wistar albino rats.

Assessment of Hepatoprotective Activity of Musa Paradisica Linn. Whole Plant Extract Against Carbon Tetrachloride Induced Hepatotoxicity in Wistar Rats

Ethonolic whole plant extract of Musa paradisica has a multiple pharmacological activities includ... more Ethonolic whole plant extract of Musa paradisica has a multiple pharmacological activities including antimicrobial, antiviral, antiinflammatory, anticancer effects. The present study aims to determine the effect of Musa paradisica on serum and tissue superoxide dismutase (SOD) levels and the histology in carbon tetrachloride (CCl4)-induced liver injury. Wister rats aged were injected Intraperitoneally with 50% CCl4 in olive oil. Musa paradisica was orally administered before or after CCl4 treatment in various groups. Twenty-four hours after CCl4 injection, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, serum and liver superoxide dismutase (SOD) activities were measured and Histological changes of liver were examined by microscopy. Serum ALT and AST activities significantly decreased in a dose-dependent manner in both pre-treatment and post-treatment groups with ethonolic whole plant extract of Musa paradisica. The present study demonstrates tha...

Recent Advances in Mucoadhesive Buccal Drug Delivery Systems and Its Marketed Scope and Opportunities

earthjournals.org

Page 1. CRITICAL REVIEW IN PHARMACEUTICAL SCIENCES Issue 1 : 2012 www.earthjournals. org 79 Revie... more

Alkaloid Based Chemical Constituents of Ocimum santum & Cinchona Bark : A Meta Analysis

Journal for Research in Applied Sciences and Biotechnology

This article provides a concise summary of the recent developments that have been achieved in our... more This article provides a concise summary of the recent developments that have been achieved in our comprehension of the asymmetric addition processes that are catalysed by native Cinchona alkaloids and their derivatives. This class of reactions includes cycloadditions, 1,4-adds, direct nucleophilic additions across carbon–oxygen or carbon–nitrogen double bonds, and direct nucleophilic additions across carbon–oxygen double bonds. Because of their capacity to catalyse the addition of a wide variety of functional groups to C9, many Cinchona alkaloids have been utilised in these processes as catalysts. These functional groups include amino, alkoxy, hydroxyl, amido, urea, and thiourea, among others. The importance of mechanical variables is emphasised in many different contexts. Additionally, the utilisation of adducts in future synthesis is sometimes broken down into its component steps. Ocimum basilicum was discovered to be mostly consisted of estragol (> 35.71 percent), (E)-ocimene ...

Download

Cystic Fibrosis: Biology and Therapeutics

Chronic Lung Diseases, 2020

Cystic fibrosis could be a common life-bound autosomal recessive hereditary condition, with highe... more Cystic fibrosis could be a common life-bound autosomal recessive hereditary condition, with highest occurrence in Europe, North America, and Australia. The root of illness is mutation of a gene that encodes a chloride-conducting transmembrane channel known as the cystic fibrosis transmembrane conductance regulator (CFTR) that regulates anion transfer and mucociliary clearance within the airways. Operational failure of CFTR ends up in mucus withholding and chronic contagion, followed by local airway swelling that is harmful to the lungs. CFTR operational impairment principally affects epithelial cells, though there is proof of a function in immune cells. Cystic fibrosis influences numerous body systems, and morbidity and mortality are typically due to bronchiectasis, tiny airways obstacle, and progressive respiratory abnormality. Necessary comorbidities due to epithelial cell operational impairment occur within the pancreas (malassimilation), liver (biliary cirrhosis), sweat glands (heat shock), and vas deferens (sterility). The progress and delivery of medication that recover the clearance of mucus from the lungs and treat the ensuing infection, together with rectification of pancreatic insufficiency and malnutrition via multidisciplinary requisites, have resulted in noteworthy enhancements of life and clinical conclusion in patients with cystic fibrosis. Inventive and transformational treatments that aim on the fundamental defect in cystic fibrosis have currently been grown and are useful in lung surgery and dropping pulmonary

Download

Chronopharmacology: As A Therapy for Cardiovascular Disease

The Pharma Innovation Journal, 2012

1. Institute of pharmaceutical research, GLA University, Mathura, India 2. Advance institute of b... more 1. Institute of pharmaceutical research, GLA University, Mathura, India 2. Advance institute of biotech & paramedical sciences, kanpur 3. Karpagam University, Coimbatore, Tamil Nadu, India Chronopharmacology is the study of the manner & extent to which the kinetics & dynamics of medication directly affected by endogenous biological rhythm & also how the dosing time of medications affects biological timekeeping & features ( period, level, amplitude & phase ) of biological rhythms. Chronopharmacology includes chronopharmacotherapy, chronopharmacokinetics & Chronotoxity. Chronopharmacotherapy is the investigative science that elucidates the biological rhythm dependencies of medication. It is useful to solve problems of drug optimization i.e. to enhance the desired efficiency or to reduce its undesired effects. So Chronopharmacologic approaches involve a lesser risk of errors and or false information than the conventional homeostatic approach. The effectiveness & toxicity of many drugs ...

Download

Cystic Fibrosis: Biology and Therapeutics

Chronic Lung Diseases, 2020

Cystic fibrosis could be a common life-bound autosomal recessive hereditary condition, with highe... more Cystic fibrosis could be a common life-bound autosomal recessive hereditary condition, with highest occurrence in Europe, North America, and Australia. The root of illness is mutation of a gene that encodes a chloride-conducting transmembrane channel known as the cystic fibrosis transmembrane conductance regulator (CFTR) that regulates anion transfer and mucociliary clearance within the airways. Operational failure of CFTR ends up in mucus withholding and chronic contagion, followed by local airway swelling that is harmful to the lungs. CFTR operational impairment principally affects epithelial cells, though there is proof of a function in immune cells. Cystic fibrosis influences numerous body systems, and morbidity and mortality are typically due to bronchiectasis, tiny airways obstacle, and progressive respiratory abnormality. Necessary comorbidities due to epithelial cell operational impairment occur within the pancreas (malassimilation), liver (biliary cirrhosis), sweat glands (heat shock), and vas deferens (sterility). The progress and delivery of medication that recover the clearance of mucus from the lungs and treat the ensuing infection, together with rectification of pancreatic insufficiency and malnutrition via multidisciplinary requisites, have resulted in noteworthy enhancements of life and clinical conclusion in patients with cystic fibrosis. Inventive and transformational treatments that aim on the fundamental defect in cystic fibrosis have currently been grown and are useful in lung surgery and dropping pulmonary

Download

Cystic Fibrosis: Biology and Therapeutics

Chronic Lung Diseases, 2020

Cystic fibrosis could be a common life-bound autosomal recessive hereditary condition, with highe... more Cystic fibrosis could be a common life-bound autosomal recessive hereditary condition, with highest occurrence in Europe, North America, and Australia. The root of illness is mutation of a gene that encodes a chloride-conducting transmembrane channel known as the cystic fibrosis transmembrane conductance regulator (CFTR) that regulates anion transfer and mucociliary clearance within the airways. Operational failure of CFTR ends up in mucus withholding and chronic contagion, followed by local airway swelling that is harmful to the lungs. CFTR operational impairment principally affects epithelial cells, though there is proof of a function in immune cells. Cystic fibrosis influences numerous body systems, and morbidity and mortality are typically due to bronchiectasis, tiny airways obstacle, and progressive respiratory abnormality. Necessary comorbidities due to epithelial cell operational impairment occur within the pancreas (malassimilation), liver (biliary cirrhosis), sweat glands (heat shock), and vas deferens (sterility). The progress and delivery of medication that recover the clearance of mucus from the lungs and treat the ensuing infection, together with rectification of pancreatic insufficiency and malnutrition via multidisciplinary requisites, have resulted in noteworthy enhancements of life and clinical conclusion in patients with cystic fibrosis. Inventive and transformational treatments that aim on the fundamental defect in cystic fibrosis have currently been grown and are useful in lung surgery and dropping pulmonary

Download

Uploads

Papers by Shravan Paswan

Log In