Papers by Channarong Sartsanga
Localization and quantitative distribution of a chromatin structural protein Topoisomerase II on plant chromosome using HVTEM and UHVTEM
Micron, Apr 1, 2024
Differential intra-host infection kinetics in Aedes aegypti underlie superior transmissibility of African relative to Asian Zika virus
mSphere
Despite numerous studies highlighting the higher transmissibility of the African Zika virus (ZIKV... more Despite numerous studies highlighting the higher transmissibility of the African Zika virus (ZIKV) lineage compared to the Asian lineage in mosquito vectors, little is known about how the viruses interact with different tissues during the early steps of mosquito infection. To address this gap, we aimed to characterize intra-host infection barriers by combining tissue-level monitoring of infection using plaque assays and a novel quantitative analysis of single-cell-level infection kinetics by in situ immunofluorescent staining. Our results revealed that, in Aedes aegypti , an African ZIKV strain exhibited a higher replication rate across various tissues than an Asian ZIKV strain. This difference was potentially due to a higher virus production in individual cells, faster spread within tissues, or a combination of both factors. Furthermore, we observed that higher blood meal titers resulted in a faster viral spread to neighboring cells suggesting that intra-host infection dynamics dep...
Description of four new Medeopteryx Ballantyne (Coleoptera, Lampyridae, Luciolinae) species from Thailand and their phylogenetic placements based on mitochondrial DNA
Journal of Asia-pacific Entomology, Jun 1, 2023
Surface structures consisting of chromatin fibers in isolated barley (Hordeum vulgare) chromosomes revealed by helium ion microscopy
Chromosome Research, Feb 22, 2021
The chromosome compaction of chromatin fibers results in the formation of the nucleosome, which c... more The chromosome compaction of chromatin fibers results in the formation of the nucleosome, which consists of a DNA unit coiled around a core of histone molecules associated with linker histone. The compaction of chromatin fibers has been a topic of controversy since the discovery of chromosomes in the 19th century. Although chromatin fibers were first identified using electron microscopy, the chromatin fibers on the surface of chromosome structures in plants remain unclear due to shrinking and breaking caused by prior chromosome isolation or preparation with alcohol and acid fixation, and critical point drying occurred into dehydration and denatured chromosomal proteins. This study aimed to develop a high-quality procedure for the isolation and preparation of plant chromosomes, maintaining the native chromosome structure, to elucidate the organization of chromatin fibers on the surface of plant chromosomes by electron microscopy. A simple technique to isolate intact barley (Hordeum vulgare) chromosomes with a high yield was developed, allowing chromosomes to be observed with a high-resolution scanning ion microscopy and helium ion microscopy (HIM) imaging technology, based on a scanning helium ion beam. HIM images from the surface chromatin fibers were analyzed to determine the size and alignment of the chromatin fibers. The unit size of the chromatin fibers was 11.6 ± 3.5 nm and was closely aligned to the chromatin network model. Our findings indicate that compacting the surface structure of barley via a chromatin network and observation via HIM are powerful tools for investigating the structure of chromatin.
Coleopterists Bulletin, Mar 23, 2018
Despite numerous studies highlighting the higher transmissibility of the African Zika virus (ZIKV... more Despite numerous studies highlighting the higher transmissibility of the African Zika virus (ZIKV) lineage compared to the Asian lineage in mosquito vectors, little is known about how the viruses interact with different tissues during the early steps of mosquito infection. To address this gap, we aimed to characterize intra-host infection barriers by combining a tissue-level monitoring of infection using plaque assays and a novel quantitative analysis of single-cell level infection kinetics byin situimmunofluorescent staining. Our results revealed that, inAedes aegypti, an African ZIKV strain exhibited a higher replication rate across various tissues than an Asian ZIKV strain. This difference was potentially due to a higher virus production in individual cells, faster spread within tissues, or a combination of both factors. Furthermore, we observed that higher bloodmeal titers resulted in a faster viral spread to neighboring cells suggesting that intra-host infection dynamics depend...
Description of four new Medeopteryx Ballantyne (Coleoptera, Lampyridae, Luciolinae) species from Thailand and their phylogenetic placements based on mitochondrial DNA
Journal of Asia-Pacific Entomology
Higher-order structure of barley chromosomes observed by electron tomography
Micron
Australoluciola Ballantyne 2013
<i>Australoluciola</i> Ballantyne, 2013 <i>Australoluciola</i> Ballantyne... more <i>Australoluciola</i> Ballantyne, 2013 <i>Australoluciola</i> Ballantyne and Lambkin 2013: 43.
Fig. 3 in Distribution of the Firefly Genus Pteroptyx Olivier and a New Record of Pteroptyx asymmetria Ballantyne (Coleoptera: Lampyridae: Luciolinae) in Thailand
Fig. 3. Pteroptyx malaccae (KU-2013-32-17). A) Dorsal habitus, B) Ventral habitus, C) Apex of abd... more Fig. 3. Pteroptyx malaccae (KU-2013-32-17). A) Dorsal habitus, B) Ventral habitus, C) Apex of abdomen (V5–V7) and elytra, ventral view, D) Dissected apex of abdomen (V5–V7), cleared, dorsal view, E) Tergite 8, ventral view, F) Intact abdominal tergites, part of wings and elytra, ventral view, G) Aedeagal sheath, dorsal view, H) Aedeagal sheath, left lateral view, I) Aedeagal sheath, ventral view, J) Aedeagal sheath with aedeagus still contained, ventral view, K) Aedeagus, dorsal view, L) Aedeagus, right lateral view, M) Aedeagus, ventral view, N) Right elytron, ventral view.
Fig. 2 in Distribution of the Firefly Genus Pteroptyx Olivier and a New Record of Pteroptyx asymmetria Ballantyne (Coleoptera: Lampyridae: Luciolinae) in Thailand
Fig. 2. Pteroptyx asymmetria (KU-2012-14-1). A) Dorsal habitus, B) Ventral habitus, C) Head and p... more Fig. 2. Pteroptyx asymmetria (KU-2012-14-1). A) Dorsal habitus, B) Ventral habitus, C) Head and part of thorax, cventral view, D) Apices of abdomen (V5–V7) and elytra, ventral view, E) Dissected apex of abdomen (V5–V7), cleared, dorsal view, F) Aedeagal sheath, dorsal view, G) Aedeagal sheath, left lateral view, H) Aedeagal sheath, ventral view, I) Tergite 8, ventral view, J) Aedeagus, ventral view, K) Aedeagus, right lateral view, L) Aedeagus, dorsal view, M) Right elytron, ventral view.
Fig. 4 in Distribution of the Firefly Genus Pteroptyx Olivier and a New Record of Pteroptyx asymmetria Ballantyne (Coleoptera: Lampyridae: Luciolinae) in Thailand
Fig. 4. Pteroptyx tener (KU-2013-28-08). A) Dorsal habitus, B) Ventral habitus, C) Apex of abdome... more Fig. 4. Pteroptyx tener (KU-2013-28-08). A) Dorsal habitus, B) Ventral habitus, C) Apex of abdomen (V5–V7) and elytra, ventral view, D) Dissected apex of abdomen (V5–V7), cleared, ventral view, E) Tergite 8, ventral view, F) Intact abdominal tergites, part of wings and elytra, ventral view, G) Aedeagal sheath, ventral view, H) Aedeagal sheath, left lateral view, I) Aedeagal sheath, dorsal view, J) Aedeagal sheath with aedeagus still contained, dorsal view, K) Aedeagus, dorsal view, L) Aedeagus, left lateral view, M) Aedeagus ventral view, N) Left elytron, ventral view.
Fig. 1 in Distribution of the Firefly Genus Pteroptyx Olivier and a New Record of Pteroptyx asymmetria Ballantyne (Coleoptera: Lampyridae: Luciolinae) in Thailand
Fig. 1. Distribution and habitat of Pteroptyx species in Thailand. A) Map of Thailand with Pterop... more Fig. 1. Distribution and habitat of Pteroptyx species in Thailand. A) Map of Thailand with Pteroptyx locations, B) Synchronous flashing display of P. malaccae in Samut Prakan, C) Estuary in Samut Songkhram, D) Mangrove forest in Trat, E) P. asymmetria habitat in Krabi.
Fig. 5 in Distribution of the Firefly Genus Pteroptyx Olivier and a New Record of Pteroptyx asymmetria Ballantyne (Coleoptera: Lampyridae: Luciolinae) in Thailand
Fig. 5. Pteroptyx valida (KU-2012-12-52). A) Dorsal habitus, B) Ventral habitus, C) Apex of abdom... more Fig. 5. Pteroptyx valida (KU-2012-12-52). A) Dorsal habitus, B) Ventral habitus, C) Apex of abdomen (V5–V7) and elytra, ventral view, D) Dissected apex of abdomen (V5–V7), cleared, dorsal view, E) Tergite 8, ventral view, F) Intact abdominal tergites, part of wings and elytron, dorsal view, G) Aedeagal sheath, ventral view, H) Aedeagal sheath, left lateral view, I) Aedeagal sheath, dorsal view, J) Aedeagal sheath with aedeagus still contained, dorsal view, K) Aedeagus, dorsal view, L) Aedeagus, right lateral view, M) Aedeagus, ventral view, N) Right elytron, ventral view.
Fig. 1 in Australoluciola thailandica Sriboonlert, Swatdipong, and Sartsanga, New Species (Coleoptera: Lampyridae: Luciolinae) from Thailand: First Record of the Genus Australoluciola Ballantyne from Southeast Asia
Fig. 1. Type locality of Australoluciola thailandica. A) Map of Thailand indicating type locality... more Fig. 1. Type locality of Australoluciola thailandica. A) Map of Thailand indicating type locality in Tak province near the Myanmar border (acquired from commons.wikimedia.org/wiki/File:Thailand_Topography.png), B) Habitat.
Fig. 2 in Australoluciola thailandica Sriboonlert, Swatdipong, and Sartsanga, New Species (Coleoptera: Lampyridae: Luciolinae) from Thailand: First Record of the Genus Australoluciola Ballantyne from Southeast Asia
Fig. 2. Australoluciola thailandica, holotype male (KU-2016-08-23). A) Dorsal habitus, B) Ventral... more Fig. 2. Australoluciola thailandica, holotype male (KU-2016-08-23). A) Dorsal habitus, B) Ventral habitus, C) Pronotum, D) Abdomen (V5–7), paratype male (KU-2016-08-24), E) Aedeagus and sheath within T8, dorsal view, F) Aedeagal sheath with aedeagus still contained, dorsal view, G) T8, ventral view, H) Head and part of thorax, ventral view, I) Aedeagal sheath, dorsal view, J) Aedeagal sheath, lateral view, K) Aedeagal sheath, ventral view, L) Left elytron, dorsal view, M) Aedeagus, dorsal view, N) Aedeagus, right lateral view, O) Aedeagus, right ventral view, P) Abdominal tergites.
Calcium ion significance on the maintenance of barley (Hordeum vulgare) chromosome compaction
Micron, 2021
Cations, especially calcium ions (Ca2+), is one of the major factors responsible for the chromoso... more Cations, especially calcium ions (Ca2+), is one of the major factors responsible for the chromosome higher-order structure formation. The effects of cations on the human chromosomes have already been evaluated, however, whether the presence of similar effects on plant chromosomes has not been reported to date. Thus, in this study, we investigated the role of Ca2+ on the barley (Hordeum vulgare L.) chromosome structure. Barley chromosomes were isolated from the meristematic tissue within the germinated roots. The roots were subjected to enzymatic treatment, fixed, and drop on the cover glass to spread the chromosomes out. Some chromosomes were treated with BAPTA (1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid) to chelate Ca2+. Chromosome samples were then observed by fluorescence microscopy and scanning electron microscopy (SEM). The disperse structure of the chromosome was observed after BAPTA treatment. Chromosomes showed less condensed structure due to Ca2+ chelation. The high-resolution of SEM provided a more detailed visualization of chromosome ultrastructure under different calcium ion conditions. This study revealed the calcium ion effect on chromosome structure is important regardless of the organisms, suggesting a similar mechanism of chromosome condensation through humans and plants.
The Coleopterists Bulletin, 2017
The discovery of Australoluciola thailandica Sriboonlert, Swatdipong, and Sartsanga, new species,... more The discovery of Australoluciola thailandica Sriboonlert, Swatdipong, and Sartsanga, new species, in a northern province of Thailand extends the range of the genus previously known only in Australia and New Guinea. A description based on three males is given, and a key to the species of Australoluciola Ballantyne is provided.
The Coleopterists Bulletin, 2018
Surface structures consisting of chromatin fibers in isolated barley (Hordeum vulgare) chromosomes revealed by helium ion microscopy
Chromosome Research
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Papers by Channarong Sartsanga