Papers by jorge rodriguez suarez
A lipid mediated mechanism increases protein O‐glycosylation in hearts of Type 2 diabetic mice
The FASEB Journal, 2008
Mitochondrial calcium is decreased by high glucose and is improved by reduction of protein O‐GlcNAcylation in cardiac myocytes (1078.2)
The FASEB Journal, 2014
Increased nuclear protein O‐linked β‐N‐acetylglucosamine glycosylation (O‐GlcNAcylation) mediated... more Increased nuclear protein O‐linked β‐N‐acetylglucosamine glycosylation (O‐GlcNAcylation) mediated by high glucose treatment or the hyperglycemia of diabetes mellitus contributes to cardiac myocyte dysfunction. Mitochondrial proteins in cardiac myocytes are also submitted to O‐GlcNAcylation, however, whether excessive O‐GlcNAcylation alters mitochondrial function is incompletely studied. We have previously demonstrated decreased mitochondrial calcium concentration in cardiac myocytes exposed to high glucose. We hypothesize that excessive O‐GlcNAcylation may be responsible of this maladaptive phenomenon and that reducing excessive O‐GlcNAcylation may return mitochondrial calcium levels to normal. Our objective was to test this hypothesis, therefore, we incubated cultured neonatal mouse myocytes in high glucose (HG, 25mM) for 72 hours and inhibited O‐GlcNAcylation by adenoviral expression of a dominant negative mutant of the O‐GlcNAc transferase (dnOGT). As expected, mitochondrial calc...
Estudio de consolidantes y protectivos para la restauración de material pétreo
Historia de las ideas, pensamiento y exilio
Ensayos Sobre La Historia Del Pensamiento Espanol Homenaje a Jose Luis Abellan 2012 Isbn 978 84 15463 22 1 Pags 189 218, 2012
Aplicación de un modelo casi ideal de demanda: el sector de bedidas no alcohólicas en Medellín, Colombia
Pregnancia de mitos del artista en estudiantes de diversas facultades de bellas artes nacionales: Perfiles psicológicos y salidas profesionales
Revista De Bellas Artes Revista De Artes Plasticas Estetica Diseno E Imagen, 2010
MEMENTO. Parte del canon de la misa, en que hace conmemoración de los difuntos
Inmanencia Revista Del Hospital Interzonal General De Agudos Eva Peron, Jul 3, 2012
Les propongo, con la gravedad de las palabras finales de la vida, que nos abracemos en un comprom... more Les propongo, con la gravedad de las palabras finales de la vida, que nos abracemos en un compromiso: salgamos a los espacios abiertos, arriesguemonos por el otro, esperemos, con quien extiende sus brazos, que una nueva ola de la historia nos levante. Quiza ya lo esta haciendo, de un modo silencioso y subterraneo, como los brotes que laten bajo las tierras del invierno. Algo por lo que todavia vale la pena sufrir y morir; una comunion entre hombres, aquel pacto entre derrotados. Una sola torre, si, pero refulgente e indestructible. En tiempos oscuros nos ayudan quienes han sabido andar en la noche. Lean las cartas que Miguel Hernandez envio desde la carcel donde finalmente encontro la muerte: Volveremos a brindar por todo lo que se pierde y se encuentra: la libertad, las cadenas, la alegria. Y ese carino oculto que nos arrastra a buscarnos. A traves de toda la tierra. Piensen siempre en la nobleza de estos hombres que redimen a la humanidad. A traves de su muerte nos entregan el valor supremo de la vida, mostrandonos que el obstaculo no impide la historia, nos recuerdan que el hombre solo cabe en la utopia. Solo quienes sean capaces de encarnar la utopia seran aptos para el combate decisivo, el de recuperar cuanto de humanidad hayamos perdido. Ernesto Sabato. Antes del Fin. Buenos Aires: Seix Barral, 1998: pp 213-214
Use of calcium binding proteins to improve cardiac contractile function
Del significado responsabilidad de los socios en las compañías mercantiles por acciones y por cuotas
Breve historia de los montes de piedad en Argentina y Latinoamérica
Pignus Revista Internacional De Credito Pignoraticio, 2004
American journal of physiology. Regulatory, integrative and comparative physiology, 2014
Ischemic heart disease (IHD) is the single most common cause of death. New approaches to enhance ... more Ischemic heart disease (IHD) is the single most common cause of death. New approaches to enhance myocardial perfusion are needed to improve outcomes for patients with IHD. Thyroid hormones (TH) are known to increase blood flow; however, their usefulness for increasing perfusion in IHD is limited because TH accelerates heart rate, which can be detrimental. Therefore, selective activation of TH effects is desirable. We hypothesized that cell-type-specific TH receptor (TR) expression can increase TH action in the heart, while avoiding the negative consequences of TH treatment. We generated a binary transgenic (BTG) mouse that selectively expresses TRα1 in endothelial cells in a tetracycline-inducible fashion. In BTG mice, endothelial TRα1 protein expression was increased by twofold, which, in turn, increased coronary blood flow by 77%, coronary conductance by 60%, and coronary reserve by 47% compared with wild-type mice. Systemic blood pressure was decreased by 20% in BTG mice after TR...
Increased sorcin expression in hypertrophied mouse hearts
Journal of Molecular and Cellular Cardiology, 2001
Pressure Overload-Induced Heart Failure Is Associated With an Increased Enzymatic O-GlcNAcylation of Calcium Handling Proteins
Journal of Cardiac Failure, 2010
Journal of Biological Chemistry, 2012
Background: DRP1 plays a significant role to control mitochondrial fission. Results: DRP1 is O-Gl... more Background: DRP1 plays a significant role to control mitochondrial fission. Results: DRP1 is O-GlcNAcylated. Increased O-GlcNAcylation augments the level of the GTP-bound active form of DRP1 and induces translocation of DRP1 from cytoplasm to mitochondria. Conclusion: O-GlcNAcylation modulates DRP1 function, which has consequences for mitochondrial function. Significance: The modulation of DRP1 function by increased overall O-GlcNAcylation could play a significant role in the development of diabetic mitochondrial dysfunction.
Loan Etymologies in Historical Method
International Journal of American Linguistics, 1985
Page 1. INTERNATIONAL JOURNAL OF AMERICAN LINGUISTICS correspondence categorize differently along... more Page 1. INTERNATIONAL JOURNAL OF AMERICAN LINGUISTICS correspondence categorize differently along another, even within the limited phonetic sets which were selected by Sapir as exemplifying regularity. This complexity ...
Human Gene Therapy, 2013
Urocortin-2 (UCn2) peptide infusion increases cardiac function in patients with heart failure, bu... more Urocortin-2 (UCn2) peptide infusion increases cardiac function in patients with heart failure, but chronic peptide infusion is cumbersome, costly, and provides only short-term benefits. Gene transfer would circumvent these shortcomings. Here we ask whether a single intravenous injection of adeno-associated virus type 8 encoding murine urocortin-2 (AAV8.UCn2) could provide long-term elevation in plasma UCn2 levels and increased left ventricular (LV) function. Normal mice received AAV8.UCn2 (5 • 10 11 genome copies, intravenous). Plasma UCn2 increased 15-fold 6 weeks and > 11-fold 7 months after delivery. AAV8 DNA and UCn2 mRNA expression was persistent in LV and liver up to 7 months after a single intravenous injection of AAV8.UCn2. Physiological studies conducted both in situ and ex vivo showed increases in LV + dP/dt and in LV -dP/dt, findings that endured unchanged for 7 months. SERCA2a mRNA and protein expression was increased in LV samples and Ca 2 + transient studies showed an increased rate of Ca 2 + decline in cardiac myocytes from mice that had received UCn2 gene transfer. We conclude that a single intravenous injection of AAV8.UCn2 increases plasma UCn2 and increases LV systolic and diastolic function for at least 7 months. The simplicity of intravenous injection of a long-term expression vector encoding a gene with paracrine activity to increase cardiac function is a potentially attractive strategy in clinical settings. Future studies will determine the usefulness of this approach in the treatment of heart failure.
Observaciones sobre la evolución fonológica del tlapaneco
Anales de antropología, 2011
Page 1. OBSERVACIONES SOBRE LA EVOLUCIÓN FONOLóGICA DEL TI,APANECO JoRcE A. SUAIM O. en una monog... more Page 1. OBSERVACIONES SOBRE LA EVOLUCIÓN FONOLóGICA DEL TI,APANECO JoRcE A. SUAIM O. en una monografía reciente, C. Rensch (1977) ha in-teg¡ado finr¡ement€ el tlapaneco dentro de su reconstruccién ...
Circulation Research, 2005
To examine whether excessive protein O -GlcNAcylation plays a role in the dysfunction of the diab... more To examine whether excessive protein O -GlcNAcylation plays a role in the dysfunction of the diabetic heart, we delivered adenovirus expressing O -GlcNAcase (Adv-GCA) into the myocardium of STZ-induced diabetic mice. Our results indicated that excessive cellular O -GlcNAcylation exists in the diabetic heart, and that in vivo GCA overexpression reduces overall cellular O -GlcNAcylation. Myocytes isolated from diabetic hearts receiving Adv-GCA exhibited improved calcium transients with a significantly shortened T decay ( P <0.01) and increased sarcoplasmic reticulum Ca 2+ load ( P <0.01). These myocytes also demonstrated improved contractility including a significant increase in +dL/dt and −dL/dt and greater fractional shortening as measured by edge detection ( P <0.01). In isolated perfused hearts, developed pressure and −dP/dt were significantly improved in diabetic hearts receiving Adv-GCA ( P <0.05). These hearts also exhibited a 40% increase in SERCA2a expression. Pho...
British Journal of Pharmacology, 2010
Urocortin 2 is beneficial in heart failure, but the underlying cellular mechanisms are not comple... more Urocortin 2 is beneficial in heart failure, but the underlying cellular mechanisms are not completely understood. Here we have characterized the functional effects of urocortin 2 on mouse cardiomyocytes and elucidated the underlying signalling pathways and mechanisms. Mouse ventricular myocytes were field-stimulated at 0.5 Hz at room temperature. Fractional shortening and [Ca 2+ ]i transients were measured by an edge detection and epifluorescence system respectively. Western blots were carried out on myocyte extracts with antibodies against total phospholamban (PLN) and PLN phosphorylated at serine-16. Urocortin 2 elicited time-and concentration-dependent positive inotropic and lusitropic effects (EC50: 19 nM) that were abolished by antisauvagine-30 (10 nM, n = 6), a specific antagonist of corticotrophin releasing factor (CRF) CRF2 receptors. Urocortin 2 (100 nM) increased the amplitude and decreased the time constant of decay of the underlying [Ca 2+ ]i transients. Urocortin 2 also increased PLN phosphorylation at serine-16. H89 (2 mM) or KT5720 (1 mM), two inhibitors of protein kinase A (PKA), as well as KN93 (1 mM), an inhibitor of Ca 2+ /calmodulin-dependent protein kinase II (CaMKII), suppressed the urocortin 2 effects on shortening and [Ca 2+ ]i transients. In addition, urocortin 2 also elicited arrhythmogenic events consisting of extra cell shortenings and extra [Ca 2+ ]i increases in diastole. Urocortin 2-induced arrhythmogenic events were significantly reduced in cells pretreated with KT5720 or KN93.
American Journal of Physiology-Heart and Circulatory Physiology, 2004
Cardiac fibroblasts (CF) express adenosine (ADO) receptors, and pharmacological evidence suggests... more Cardiac fibroblasts (CF) express adenosine (ADO) receptors, and pharmacological evidence suggests the possible involvement of the A2(A2aand A2b) receptor (A2aR and A2bR) subtypes in inhibiting cell functions involved in fibrosis. The main objective of this study was to define the contributions of A2aand/or A2breceptors in modulating ADO-induced decreases in CF functions. For this purpose, CF were either treated pharmacologically or had the A2aR or A2bR levels modified through the use of recombinant adenovirus or siRNA. The assessment of mRNA expression in adult rat CF yielded evidence for A1R, A2bR, A2aR, and A3R. Endogenously or exogenously enhanced ADO significantly inhibits CF proliferation, collagen, and protein synthesis. A2R and A2aR agonists, although capable of inhibiting CF protein and collagen synthesis, were unable to define the contributions derived from A2aR or A2bR. Overexpression of A2bR in CF yielded significant decreases in basal levels of collagen and protein synth...
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Papers by jorge rodriguez suarez