Papers by prabakaran kalaivanan
The isolation isorhamnetin 3-O-α-L-(6''-E-p-coumaroyl)-rhamnoside was obtained from Indigofera ti... more The isolation isorhamnetin 3-O-α-L-(6''-E-p-coumaroyl)-rhamnoside was obtained from Indigofera tinctoria. Indigofera tinctoria flower has been found to rich in medicinal properties such as anti-inflammatory anticancer, anti diabetic, antimicrobial activity. The plant flower have been found to rich in nutrients and anti nutrients such as crude protein The present study has been under taken with an objective to determine the anti-inflammatory or immunosuppressive agent., antibacterial, antioxidant and cytotoxic activity of the flower methanol extract Indigofera tinctoria. The selected medicinal plant was collected from nearby region of cuddlaore. The compound present in isorhamnetin 3-O-α-L-(6''-E-p-coumaroyl)-rhamnoside the extract were identified by analysis of UV, IR, 1 H NMR, 13 C NMR, HPLC, TLC spectroscopy methods .
Memecylon edule (Melastamataceae family) is a flower extract with botanical and phytochemical pro... more Memecylon edule (Melastamataceae family) is a flower extract with botanical and phytochemical properties. The current work used a bioassay-guided isolation technique to examine the spectrum characterization, antibacterial activity antibiofilm from the memecylon module flowers. Plants used in traditional medicine are a great source for the discovery of novel therapeutic substances. The purpose of this study was to determine the anti-microbial effects of an EtOAc extract of Memecylon edule. Furthermore, the compound's mode of action must be examined by extracting the extract and analyzing the active element that plays the most important function. Different spectrum analyses, including The functional groups in the extract of memecylon edule study were also identified by further UV, FT-IR, NMR, and GC-MS analysis, were used to determine the structure of the isolated chemical analysis. The findings of this study imply that bioactive chemicals can be used in the pharmaceutical sector to produce plant-based medications to treat illnesses caused by microbes.
The Hygrophila auriculata found in Cuddalore (Tamil nadu) is regarded as an important medicinal p... more The Hygrophila auriculata found in Cuddalore (Tamil nadu) is regarded as an important medicinal plant. The current investigation assessed the antioxidant and antibacterial properties of H. auriculata methanol flowers extract.The phytochemicals in the plant extract were studied using GC-MS, UV, FT-IR, and NMR. There have been qualitative phytochemical tests performed on actively retorted plants. In DPPH radical scavenging tests, H.auriculata extracts were measured for their anti-oxidant activity in solvents with varying polarities. By using the agar well diffusion technique, these extracts were tested for the existence of antimicrobial activity against the Staphylococcus and Escherichia coli, Staphylococcus aureus, Bacillus subtillis, Sarcina lutea and E. coli, Pseudomonas aeruginosa and Candida albicans
Launaea nudicaulis is a significant medicinal plant with anti-inflammatory, anti-cancer, antidiab... more Launaea nudicaulis is a significant medicinal plant with anti-inflammatory, anti-cancer, antidiabetic and antibacterial properties. In the present study, we have studied the phytochemical analysis of Launaea nudicaulis leaf extract by using NMR and GC-MS technique. According to the preliminary phytochemical analysis, identify the various active ingredients. For the purposes GC-MS study was suggested to look into the organic compounds of the unique drug Launaea nudicaulis. From the results, Launaea nudicaulis' ethanolic whole plant extract was found 11 different phytochemical compounds. The GC-MS analysis confirmed their presence various Phenyl methyl esters of 6, 9, and 12-octadecatrienoic acid (368) and Phytol (296) Molecular weight compounds. Squalene, an important chemical, was identified in the fraction using a GC-MS analysis, with an 80% match rate. By analyzing the extract using spectroscopy, 1 HNMR 13 C NMR and GC-MS techniques, the compound squalene could be identified.
An efficient new approach toward the synthesis of 2-methylperlolidine alkaloids has been illustra... more An efficient new approach toward the synthesis of 2-methylperlolidine alkaloids has been illustrated, the present article describes the synthesis, in Antileukemic activities and in-silico molecular docking studies of compound 2-methylperlolidine 4. The synthesis of 4 is initiated by a new, efficient, solvent-free Minisci radical reaction. The structures of the compounds are established using both spectral and analytical data. An in-silico prediction of activity spectra for substances, the Swiss ADME-assisted docking approach is found to be suitable to derive and synthesize effective receptor tyrosine kinase agents. Claisen ester condensation reaction resulted in the discovery of inexpensive and user-friendly solvents. Structures of the newly synthesized compounds were characterized by FT-IR, 1 H NMR, 13 C NMR, and HRMS (FTMS + PESI) analyses. Molecular docking was investigated to determine the probable binding mode. The experimental values and docking simulation exhibited that the complex had better anti-leukemic than the positive reference 2-methylperlolidine.
Thespesia populnea (Fabaceae) is a native plant species of many Asian countries, including Malays... more Thespesia populnea (Fabaceae) is a native plant species of many Asian countries, including Malaysia and India. The root, stem, bark, and flowers of Thespesia populnea are used to treat various ailments, including ulcers and stomach cancer, we studied the antiulcer activity of lipid-soluble extract of Thespesia populnea obtained via extraction of air-dried flowers using chloroform. Anti ulcer activity of Chloroform extract was studied by pylorus ligated, aspirin induced gastric ulceration in albino rats the antiulcer activity chloroform of an extract of Thespesia populnea flowers was investigated by agar well diffusion method. Furthermore, our phytochemical studies indicated that the chloroform extract of Thespesia populnea flowers contains flavonoids, alkaloids, tannins and anthroquinone, glycosides. The anti ulcer activity was assessed by determining the ulcer index, pH and acidity. Chloroform extract (200mg/kg) produced a significant reduction in the ulcer index, PH and acidity when compared with ulcer control group and standard group. Chloroform extract reduces ulcer, there by the present study indicates that Thespesia populnea flowers showed Protective activity on gastric ulcer
In the present investigation a new class of pyrazole, pyrazolone and its derivatives containing q... more In the present investigation a new class of pyrazole, pyrazolone and its derivatives containing quinoline moiety have been designed and synthesized from available starting materials aniline and ethyl acetoacetate with thermodynamic favoured condensation followed by cyclization, chlorination, nucleophilic hydrazination and condensation reaction to obtain goal and characterized by FT-IR, 1 H-NMR and 13 C-NMR spectroscopy in good yield.
The present work deals with, Synthesis of quinoline Schiff's bases; they were characterized by FT... more The present work deals with, Synthesis of quinoline Schiff's bases; they were characterized by FT-IR, 1 H-NMR, 13 C-NMR spectroscopy and Mass spectrometry in good yield. Among the four Schiff's bases, N,N'-(butane-1,4-diylidene)bis(quinolin-2amine and its derivatives (3 ad) were screened for antibacterial activity and the results are described.
A chemical investigation on the leaves of Lawsonia inermis led to the isolation and identificatio... more A chemical investigation on the leaves of Lawsonia inermis led to the isolation and identification of five known and rare flavonoids, apigenin (1), 5, 7, 4-trihydroxy-6, 3, 5-trimethoxyflavone (2), pectolinarigenin (3), apigenin-7-O-β-D-glucoside (4), and pectolinarin (5). Their structures were established on the basis of chromatography and spectroscopic evidence and comparisons with literature data. Antimicrobial and carrageenan induced paw edema in male albino rats were determined. Compounds 4 and 5 showed the most powerful antimicrobial effect against Escherichia coli and Aspergillus flavus at the dose of 200 mg/kg. Compound 2 showed pronounced anti-inflammatory effects (3.17±0.15 after 3 h of injection), slightly higher than that of other four compounds.
Objective: Medicinal plants play an important role in the management of diabetes. The main object... more Objective: Medicinal plants play an important role in the management of diabetes. The main objective of the study was designed to screen the phytochemical constituents of the leaves of Prenanthes sarmentosus and also to study its effect on blood glucose of rats. Methods: The compound oncocalyxone A isolated from Prenanthes sarmentosus leaves by using Column chromatography. Structure of the compound was identified by HPLC analysis, UV, IR, NMR spectroscopic methods and tested for anti-diabetic activity in alloxan induced diabetes in albino rats was evaluated. Albino rats weighing about 200-250 g were divided into five groups (n=6). The weights of the animals in each group were recorded daily throughout the duration of the experiment. Biochemical parameters, including glucose, serum urea, creatinine, cholesterol, and protein were also assessed. The blood glucose levels in the different groups were assayed. Results: The isolated oncocalyxone A increased the body weight and significantly reduced the glucose level at 200 mg/kg was almost equally the reference standard glibenclamide. In our present study, biochemical parameters were unchanged in non-diabetic rats. Conclusion: The oncocalyxone A has the potential to enhance the glucose dependent insulin release from the pancreatic β cells and there by decrease the blood glucose level in alloxan induced diabetic rats. Further biochemical investigations will clearly elucidate the mechanism of action and will be helpful this compound as a therapeutic target in diabetes research.
Root extracts of Pavonia odorata has to investigate the bioactive compounds using GC-MS analysis.... more Root extracts of Pavonia odorata has to investigate the bioactive compounds using GC-MS analysis. It revealed the existence of 9 compounds. The present investigation was undertaken by comparative study of antimicrobial and anti-inflammatory activity between chloroform, ethyl acetate and methanolic extracts. The chloroform and ethyl acetate extract has excellent activity against Staphylococcus aureus (24 ± 0.69 & 22 ± 1.38 mm) and Candida albicans (19 ± 0.24 & 16 ± 1.02 mm). The anti-inflammatory activity of root extracts, Carrageenan-induced rat paw edema has frequently used to assess the anti-inflammatory effects of natural products. Chloroform extract has higher activity than ethyl acetate extract. It may be due to the presence of bioactive components.
Clitoria ternatea belonging to the family Fabaceae, commonly known as Butterfly pea. A wide range... more Clitoria ternatea belonging to the family Fabaceae, commonly known as Butterfly pea. A wide range of secondary metabolites including triterpenoids, flavonol glycosides, anthocyanins and steroids were isolated from Clitoria ternatea Linn. GC-MS analyses of CHCl3 and EtOAc fractions were performed on Gas Chromatography with mass spectrometer. The aim of the present study was to evaluate the radical scavenging ability, using the stable radical DPPH and anti-diabetic activity in alloxan induced diabetes in albino rats was evaluated from the flowers extract of Clitoria ternatea. The blood glucose level in the different groups was assayed and biochemical parameters were assessed to support the proposed hypothesis. During the experimental period, body weight of all the rats was determined at regular intervals of time. The serum glucose lowering activity was compared with glibenclamide, a standard hypoglycemic drug.
The aim of the present study was to evaluate the anti-bacterial, analgesic and anti-inflammatory ... more The aim of the present study was to evaluate the anti-bacterial, analgesic and anti-inflammatory activities with oncocalyxone A isolated from the leaves of Prenanthes sarmentosus. The anti-bacterial activity of different concentrations of oncocalyxone A (100, 200 mg) was evaluated against four bacterial species, namely Staphylococcus aurous, Escherichia coli, Aspergillus niger and Aspergillus flavus. Escherichia coli showed the highest susceptibility to this compound. The oral dose of oncocalyxone A at a 200mg/kg exhibited analgesic and anti-inflammatory activities in comparison with standard drug Morphine sulphate at a dose of 5 mg/kg and Diclofenac sodium at a dose of 100 mg/kg respectively. The methanolic extract of Prenanthes sarmentosus, exhibited a weak anti-bacterial, analgesic and anti-inflammatory activities in comparison with isolated oncocalyxone A. The oncocalyxone A at dose of 200mg/kg showed highly significant activities as compared to standard drugs.
Leucas aspera (Willd.) Linn. (Lamiaceae), commonly known as "Thumbai," is distributed throughout ... more Leucas aspera (Willd.) Linn. (Lamiaceae), commonly known as "Thumbai," is distributed throughout India. This study includes the isolation and characterization of flavonoids present in Leucas aspera flowers were compared wound healing activity with standard soframycin ointment. The plant materials were extracted with 95% methanol, petroleum ether, ethyl acetate, and were subjected to column and TLC chromatographic separation analysis. The chemical constituents isolated from the flowers of L. aspera were characterized based on chemical tests and spectral analysis such as UV and NMR spectroscopy. The structure of the isolated compound was confirmed as baicalein-7-O-β-D-glucuronide (baicalin). It has been investigated for wound healing activity by applying on the albino rats. A slow rate of healing was observed earlier which turns to very rapid on the 12 th day. The histopathological examination provided additional evidence for the experimental wound healing studies. Protease is a biochemical marker and pH measured to support the wound healing activity. We conclude that the baicalin isolated with the flowers of L. aspera has better wound healing activity.
Introduction: Plant has been used for the treatment of diseases all over the world and is known t... more Introduction: Plant has been used for the treatment of diseases all over the world and is known to contain substances that can be used for therapeutic purposes or as precursors for the synthesis of useful drugs. Objective: To analyses chloroform extract of Cyperus rotundus rhizomes using GC-MS and to evaluate anti-oxidant, anti-microbial activities. Method: The dried rhizomes of C. rotundus extracted with 90% methanol (MeOH) (4 X 500 ml) under reflux and has subjected to column chromatography. The anti-oxidant activity of C. rotundus rhizomes extracts in solvents of varying polarity has measured radical scavenging ability, using the stable radical, DPPH. The different extracts of C. rotundus rhizomes has evaluated for anti-microbial activity against clinical isolates of Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Aspergillus niger, Aspergillus flavus and Candida albicans has selected based on anti-microbial activity of available drugs. Result: GC-MS analysis led to identification of five compounds namely (1) Trans-(2-chlorovinyl) dimethylethoxysilane, (2) 5-hydroxymethyl furfural, (3) Vanillin lactoside, (4) 2-propenoic acid, 3-(4-hydroxy-3-methoxy phenyl)-methyl ester, (5) 9, 12, 15-octadeca trienoic acid, 2, 3-bis [(trimethyl) oxy] propyl ester is the first report in the chloroform extracts of Cyperus rotundus rhizome. Conclusion: The results showed the CHCl 3 fraction of C. rotundus rhizomes can be considered as good sources of natural antioxidants and antimicrobial compounds present in it and has incorporated into the drug theories.
Flavonoids were isolated from Salvia leucantha using column and thin layer chromatography separat... more Flavonoids were isolated from Salvia leucantha using column and thin layer chromatography separation techniques. By comparing their melting points, R f values and UV, 1 H-NMR, 13 C-NMR spectral data proved it to be quercetin-3-O-α-L-rhamnopyranosyl-(1 6)-β-D-glucopyranoside. The present study is aimed to investigate the agar diffusion antibacterial and wound healing activities of isolated compound against different microorganisms and excision wound model in albino rats, respectively. The results revealed most valuable information and also support the continued sustainable use of quercetin glycoside isolated from Salvia leucantha in traditional system of medicine.
Calotropis gigantea white (Asclepiadaceae), is a weed plant commonly known as giant milkweed. It ... more Calotropis gigantea white (Asclepiadaceae), is a weed plant commonly known as giant milkweed. It has one of the important traditional medicines to treat various ailments. The aim of this study to screen the phytochemicals present in the flower extract by GC-MS analysis. The results showed the presence of phytochemicals of alkaloids, tannins, phenol, flavonoids, sterols, anthraquinones, proteins and quinones in the flower extract. The GC-MS analysis of the extract revealed the presence of 4 major compounds. This study forms a basis of biological characterization and the importance of the compounds identified and creates many bioactive ingredients to treat many diseases.
Flavonoids were isolated from flowers of Salvia leucantha using chromatography separation techniq... more Flavonoids were isolated from flowers of Salvia leucantha using chromatography separation techniques. The structure of the isolated compound was analyzed by chemical tests and spectroscopic methods such as UV, 1 H-NMR, and 13 C-NMR. The spectral data's proved its structure confirmed as Quercetin-3-O-α-L-rhamnopyranoside-2-gallate. The present study was to evaluate the agar diffusion antibacterial against gram positive and gram negative microorganisms and wound healing activities to excision wound model in albino rats, respectively. The highest inhibitory zone was observed in S. aurous (28mm), and E. coli (24mm). The results revealed most valuable information and also support the continued sustainable use of Quercetin-3-O-α-L-rhamnopyranoside-2-gallate isolated from Salvia leucantha in traditional system of medicine for future development of new antibiotic agents.
Leucas aspera (Willd.) Linn. (Lamiaceae), commonly known as “Thumbai,” is distributed throughout ... more Leucas aspera (Willd.) Linn. (Lamiaceae), commonly known as “Thumbai,” is distributed throughout India. This study includes the isolation and characterization of flavonoids present in Leucas aspera flowers were compared wound healing activity with standard soframycin ointment. The plant materials were extracted with 95% methanol, petroleum ether, ethyl acetate, and were subjected to column and TLC chromatographic separation analysis. The chemical constituents isolated from the flowers of L. aspera were characterized based on chemical tests and spectral analysis such as UV and NMR spectroscopy. The structure of the isolated compound was confirmed as baicalein-7-O- β-D-glucuronide (baicalin). It has been investigated for wound healing activity by applying on the albino rats. A slow rate of healing was observed earlier which turns to very rapid on the 12 th day. The histopathological examination provided additional evidence for the experimental wound healing studies. Protease is a bioc...
Flavonoids were isolated from Salvia leucantha using column and thin layer chromatography separat... more Flavonoids were isolated from Salvia leucantha using column and thin layer chromatography separation techniques. By comparing their melting points, Rf values and UV, 1 H-NMR, 13 C-NMR spectral data proved it to be quercetin - 3 - O - α - L-rhamnopyranosyl - (1 6) - β - D - glucopyranoside. The present study is aimed to investigate the agar diffusion antibacterial and wound healing activities of isolated compound against different microorganisms and excision wound model in albino rats, respectively. The results revealed most valuable information and also support the continued sustainable use of quercetin glycoside isolated from Salvia leucantha in traditional system of medicine.
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Papers by prabakaran kalaivanan