A method for the quantification of S-RNase levels in single styles of self-incompatible Solanum c... more A method for the quantification of S-RNase levels in single styles of self-incompatible Solanum chacoense was developed and applied toward an experimental determination of the S-RNase threshold required for pollen rejection. It was found that, when single style values are averaged, accumulated levels of the S 11and S 12 -RNases can differ up to 10-fold within a genotype, while accumulated levels of the S 12 -RNase can differ by over 3-fold when different genotypes are compared. Surprisingly, the amount of S 12 -RNase accumulated in different styles of the same plant can differ by over 20-fold. A low level of 160 ng S-RNase in individual styles of fully incompatible plants, and a high value of 68 ng in a sporadic self-compatible (SSC) line during a bout of complete compatibility was measured, suggesting that these values bracket the threshold level of S-RNase needed for pollen rejection. Remarkably, correlations of S-RNase values to average fruit sets in different plant lines displaying sporadic self-compatibility (SSC) to different extents as well as to fruit set in immature flowers, are all consistent with a threshold value of 80 ng S 12 -RNase. Taken together, these results suggest that S-RNase levels alone are the principal determinant of the incompatibility phenotype. Interestingly, while the S-RNase threshold required for rejection of S 12 -pollen from a given genetic background is the same in styles of different genetic backgrounds, it is different when pollen donors of different genetic backgrounds are used. These results reveal a previously unsuspected level of complexity in the incompatibility reaction.
This paper is dedicated to the memory of our ATLAS colleagues who did not live to see the full im... more This paper is dedicated to the memory of our ATLAS colleagues who did not live to see the full impact and significance of their contributions to the experiment.
This paper summarizes the current views on coping styles as a useful concept in understanding ind... more This paper summarizes the current views on coping styles as a useful concept in understanding individual adaptive capacity and vulnerability to stress-related disease. Studies in feral populations indicate the existence of a proactive and a reactive coping style. These coping styles seem to play a role in the population ecology of the species. Despite domestication, genetic selection and inbreeding, the same coping styles can, to some extent, also be observed in laboratory and farm animals. Coping styles are characterized by consistent behavioral and neuroendocrine characteristics, some of which seem to be causally linked to each other. Evidence is accumulating that the two coping styles might explain a differential vulnerability to stress mediated disease due to the differential adaptive value of the two coping styles and the accompanying neuroendocrine differentiation. ᭧ Neuroscience and Biobehavioral Reviews 23 925-935 PERGAMON NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS NBR 376 0149-7634/99/$ -see front matter ᭧
Forward Looking Infrared Radiometer (FLIR) cameras offer a unique view of explosive volcanism by ... more Forward Looking Infrared Radiometer (FLIR) cameras offer a unique view of explosive volcanism by providing an image of calibrated temperatures. In this study, 344 eruptive events at Stromboli volcano, Italy, were imaged in 2001–2004 with a FLIR camera operating at up to 30 Hz. The FLIR was effective at revealing both ash plumes and coarse ballistic scoria, and a wide range of eruption styles was recorded. Eruptions at Stromboli can generally be classified into two groups: Type 1 eruptions, which are dominated by coarse ballistic particles, and Type 2 eruptions, which consist of an optically-thick, ash-rich plume, with (Type 2a) or without (Type 2b) large numbers of ballistic particles. Furthermore, Type 2a plumes exhibited gas thrust velocities (>15 m s−1) while Type 2b plumes were limited to buoyant velocities (−1) above the crater rim. A given vent would normally maintain a particular gross eruption style (Type 1 vs. 2) for days to weeks, indicating stability of the uppermost conduit on these timescales. Velocities at the crater rim had a range of 3–101 m s−1, with an overall mean value of 24 m s−1. Mean crater rim velocities by eruption style were: Type 1 = 34 m s−1, Type 2a = 31 m s−1, Type 2b = 7 m s−1. Eruption durations had a range of 6–41 s, with a mean of 15 s, similar among eruption styles. The ash in Type 2 eruptions originates from either backfilled material (crater wall slumping or ejecta rollback) or rheological changes in the uppermost magma column. Type 2a and 2b behaviors are shown to be a function of the overpressure of the bursting slug. In general, our imaging data support a broadening of the current paradigm for strombolian behavior, incorporating an uppermost conduit that can be more variable than is commonly considered.
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