In this study, we review the synthesis of copper nanoparticles (CuNPs) and their prospective uses... more In this study, we review the synthesis of copper nanoparticles (CuNPs) and their prospective uses as antioxidants, antimicrobials, and anticancer agents in the pharmaceutical sector. Copper nanoparticles could be created through a variety of methods, including chemical reduction, green synthesis, physical methods, electrochemical deposition, and the microemulsion method. These methods make it possible to precisely create nanoparticles with the necessary shapes, sizes, and surface properties, which in turn affect how well they perform biologically. CuNPs have strong antioxidant properties because they can scavenge reactive oxygen species (ROS) and prevent oxidative damage. In addition to their antioxidant properties, CuNPs show antibacterial activity against a number of microbes, including bacteria, fungi, and viruses. The potential of CuNPs as an anticancer agent has also been extensively investigated. These nanoparticles have toxic effects on a variety of cancer cell lines by induc...
We investigated the effects of phytochemical, ascorbic acid and total phenolic content on reducti... more We investigated the effects of phytochemical, ascorbic acid and total phenolic content on reduction of silver ions from plant materials and the toxicity of these silver ions on Drosophila melanogaster as an in vivo model system. In the presence of ascorbic acid concentration 38.88µg/gm and 35.5088µg/gm and the total phenolic contents concentration 97.89µg/gm and 26.99µg/gm in Chenopodium and Marigold extracts respectively, the multiple plasmon peaks were observed at the range of 360-426nm with absorbance of 0.780-0.925cm-1 in Chenopodium extract with absorbance 0.392-0.569 cm-1 in marigold extract. The appearance of multiple surface plasmon peaks indicated the spherical shape of silver nanoparticles (primary characterization). Obtained multiple plasmon resonances at 360-426nm and 310-450nm in Chenopodium and Marigold extracts, respectively may be indication of maximum reduction of Ag + and this enhancement of maximum reduction of Ag + due to the presence of ascorbic acid and total p...
This study describes the use of an extract from Cassia stula Linn (Cf) leaves to produce biogenic... more This study describes the use of an extract from Cassia stula Linn (Cf) leaves to produce biogenic copper oxide nanoparticles (CuONPs) employing a green synthesis approach. UV-Vis, FTIR, DLS, XRD and TEM studies are implemented to characterize the synthesized CfBio-CuONPs. A maximum peak was produced by the CfBio-CuONPs at 272 nm. The CfBio-CuONPs were crystalline, according to XRD measurements. The O-H group, aromatic group, and other functional groups were present in the FTIR spectrum of CfBio-CuONPs. The spherical, 15-25 nm-diameter CfBio-CuONPs were investigated using TEM to determine their size and shape. The antibacterial potential of the synthesized CfBio-CuONPs was then examined against four pathogenic bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, Bacillus subtilis and it was found that E. coli had the highest zone of inhibition (282.4), followed by B. subtilis (272.01), P. aeruginosa (240.97), and S. epidermidis (231.2). The maximum antioxidant activity (73% 1.54%) of CfBio-CuONPs was detected at a dose-dependent concentration of 2000 g/ml. On the other hand, the toxtrak test was used to determine the in vitro toxicity of CfBio-CuONPs and evaluate the percentage inhibition (%PI). According to the data, CfBio-CuONPs have a substantially stronger toxic effect value/PI against E. coli (93.52%) than against P. aeruginosa (92.65), B. subtilis (91.25%), and S. epidermidis (82.89%). These results also show that CfBio-CuONPs are more effective in killing harmful bacteria than antibiotics. Overall, the results of this study demonstrate that CfBio-CuONPs are superior to chloramphenicol and close to gentamycin in their ability to eliminate pathogenic microorganisms. The ecotoxicological consequences may bene t from these ndings.
Editorial on the Research Topic Recent trends in nanotechnology in precision and sustainable agri... more Editorial on the Research Topic Recent trends in nanotechnology in precision and sustainable agriculture The goal of this research on contemporary advancements in nanotechnology for precision and sustainable agriculture is to improve agricultural practices by making use of the special qualities of nanomaterials, nanoparticles, and nano devices. Nanotechnology provides creative solutions for the problems encountered by agriculture by increasing precision in monitoring soil conditions and insect infestations, optimising resource utilisation, and minimising environmental effects (Tyagi et al., 2021; Yadav et al., 2023) Furthermore, nanotechnology can help in the development of crop types that are resilient to stress, allowing farmers to adjust to climate change and guarantee long-term sustainability in food production. The goal of the investigation of nanotechnology in agriculture is to develop farming methods that are more robust, ecologically friendly, and effective. In conventional farming, over one-third of crops are lost due to insect infestation, microbial attacks, natural disasters, poor soil quality, and decreased nutrient availability. To solve these problems, novel technologies are required (Haris et al., 2023). The agro technological revolution and sustainable agriculture, which have the potential to change the agricultural system and provide food security, have been made possible by precision nanotechnology. The indiscriminate use of pesticides and chemical fertilisers, which diminished soil biodiversity and created resistance to diseases and pests, was a problem that the green revolution helped to address. For precision farming, improved biosensors may be made using nanomaterials, nanoparticles, and nanochips to transport ingredients to plants. Traditional fertilisers, pesticides, and herbicides that have been nanoencapsulated aid in the delayed and sustained release of nutrients and agrochemicals, giving the plants precise amounts. Plant growth regulators and immune system enhancers are two recent commercial applications of nano fertilisers (Zaim et al., 2023). They contain essential elements (such as iron, titanium dioxide, silica, and zinc) that play critical roles in plant growth, nutrient absorption, and disease resistance. Their presence in the soil ensures that crops receive the nutrients they require for optimal growth and yield. Pests frequently infest agricultural fields and reduce agricultural productivity. The development of nanoencapsulated insecticides has reduced the pesticide dosage. Therefore, the development of Frontiers in Plant Science frontiersin.org 01
Silver Nanoparticles Biosynthesis, Structural Characterization, Antibacterial Action, and Antioxidant Potential
ECS Transactions
The goal of this work is to address the green synthesis of silver nanoparticles (AgNPs) from Cass... more The goal of this work is to address the green synthesis of silver nanoparticles (AgNPs) from Cassia occidentalis leaf aqueous extract. The AgNPs were studied using UV–visible spectroscopy, transmission electron microscopy, scanning electron microscopy, and X-ray diffraction. The spectra of AgNPs had a single strong peak at 435 nm, but the spectra of extract had two peaks at 230 and 250 nm, indicating the presence of polyphenols and phytochemicals in the solution, and the X-ray diffraction patterns showed that they were crystals. Obtained nanoparticles are round and oval in shape, according to TEM and SEM investigations, with diameters ranging from 6 to 30 nm. Pathogenic microorganisms such as E. coli and B. subtilis were used to investigate AgNPs antibacterial efficacy. As a consequence, it was discovered that green AgNPs exhibit significant antibacterial activity.
In the current investigation, silver/gold nanoparticles (NPs) were synthesized using two methods:... more In the current investigation, silver/gold nanoparticles (NPs) were synthesized using two methods: chemical and biological, and then characterized colloidal solutions of both NPs using UV-Vis, transmission electron microscopy (TEM) and zeta potential analyzers, X-ray powder diffraction (XRD), and energy dispersive X-ray (EDX) as well as the ToxTrak test for in vitro toxicity and antibacterial activity against Gram-positive bacteria (B. subtilis) and Gram-negative bacteria (E. coli). The plasmon peak of chemical synthesized silver NPs (CH-AgNPs) and gold NPs (CH-AuNPs) was observed at 414 and 530 nm, respectively, while the sharp plasmon peak of biological synthesized silver NPs (Bio-AgNPs) and gold NPs (Bio-AuNPs) was observed at 410 and 525 nm. Under transmission electron microscopy (TEM), the average sizes of CH-AgNPs and CH-AuNPs were 50.56 and 25.98 nm, respectively. Bio-AgNPs and Bio-AuNPs, on the other hand, had average sizes of 25.25 and 16.65 nm, respectively. The stability o...
Silver nanoparticle synthesis of the leaf extract Tagetes erecta L. enriched with ascorbic acid a... more Silver nanoparticle synthesis of the leaf extract Tagetes erecta L. enriched with ascorbic acid and polyphenols has been investigated. The color of the golden yellow extract has changed to pinkish-brown due to the reduction of Ag+ to the colloidal solution of AgNPs and a sharp absorption peak at 420 nm under the UV-Vis spectrophotometer. In addition, the Fourier Transfer Infrared Spectroscopy (FTIR) estimation was completed in order to recognize and identify the biomolecules present in the extract acting as a reducing and capping agent for the AgNPs. The X-ray diffraction (XRD) peaks at (111), (201), (220), and (311) confirm the presence of monoclinic crystals in the solution. The morphology and size of the particles were provided by transmission electron microscopy (TEM) images of AgNPs. At a scale of 100 nm, synthesized AgNPs were predominantly spherical with a size range of 7-35 nm. In comparison to 7.39 mg/100 g in AgNPs, aqueous leaf extract was 55.14 mg/100 g higher in ascorbi...
Randomly selected 80 samples of open air/surface samples 40 samples each of kitchens and living r... more Randomly selected 80 samples of open air/surface samples 40 samples each of kitchens and living rooms respectively from 80 homes ( 40 homes of rural and 40 homes of urban areas) of Meerut district from July to August 2013. Higher bacterial growth (97.5%) in kitchens of rural areas as compared to that of the urban areas (95%) was reported. The higher bacterial growth (97.5%) was observed in living rooms of rural areas as compared to the living rooms of urban areas (87.5%). The results of this work indicated that the rural kitchens are more contaminated with 7 genera of pathogenic virulent bacteria i.e. Klebsiella spp. (97.5%), Shigella spp. (90%), Salmonella spp. (87.5%), Proteus spp. (87.5%), Citrobacter spp. (45%) Enterobacter spp. (27.5%) and Aeromonas spp. (20%) as compared to urban kitchens in which, only 3 genera of pathogenic virulent bacteria i.e. Acinetobacter spp. (80%), Streptococcus spp. (35%) and Corynebacterium spp. (22.5%) are found to share human habitat. On the other...
Spinacia oleracea (spinach) and Musa acuminata (banana) were chosen for the study, and aqueous ex... more Spinacia oleracea (spinach) and Musa acuminata (banana) were chosen for the study, and aqueous extracts of spinach leaf extract (SLE) and banana peel extract (BPE) were prepared for the synthesis of iron nanoparticles (FeNPs), and their antibacterial potential against pathogenic bacteria Bacillus subtilis (MTTC 1133) and Escherichia coli (MTTC 62) was evaluated. In 10 minutes at 60°C, the color of the mixture (FeCl3+SLE) changed from light green to dark blackish-brown, and the color of the mix (FeCl3+BPE) changed from transparent yellow to dark black, confirming the synthesis of FeNPs from SLE and BPE, respectively. The UV-Vis spectra of spinach- and banana-derived FeNPs revealed two peaks ranging from 240 to 430 nm and multiple peaks at 240, 270, and 395 nm, respectively. FTIR spectroscopy was used to show different functional groups on BPE and SLE, and their role in FeNP synthesis was predicted. TEM micrographs showed that the particles were in nanoscale, ranging in size from 20 t...
Beware! Our home is Wonderland of Pathogenic Bacteria
The number of bacteria isolates from living rooms in 55 (bacterial growth was observed in 39 plat... more The number of bacteria isolates from living rooms in 55 (bacterial growth was observed in 39 plates out of 40 plates only 1 plate were devoid of bacteria) in rural areas and 41 (bacterial growth was observed in 37 plates out of 40 plates 3 plate are devoid of bacteria) in urban areas was recorded. The present investigation clearly indicates that the living rooms of rural areas are more contaminated (with the major fractions of 4 genera such as Proteus spp., Salmonella spp., Shigella spp., Klebsiella spp.) in comparison to urban areas (3 genera: Acinetobacter spp., Klebsiella spp. and Proteus spp.). All in all, the rural living rooms are more contaminated with pathogenic virulent bacteria as compare to urban living rooms. On the other hand, we can say that our home is wonderland of phatogenic bacteria share with our habitat. The results of this study will help the awareness of residing populations in rural and urban areas to make an educated decisions and precautions in developing a ...
Full Length Research Article ESTIMATION OF SLIVER NANOPARTICLES TOXICITY ON HUMAN GUT MICRO FLORA
We synthesized silver nanoparticles ( AgNPs ) by using the biological material, the extract of Al... more We synthesized silver nanoparticles ( AgNPs ) by using the biological material, the extract of Allium sativum (Garlic) and chemically synthesized AgNPs by using silver nitrate. The reduction of pure Ag + ions was monitored by measuring the UV - Vis s pectrum of the reaction medium at 2 hours after diluting a small aliquot of the sample into distilled water. Toxicity of AgNPs was tested using toxtrak test in which, fresh overnight broths of Bacillus subtilis and resazurin dye was used to calculate percentage inhibition (PI). The PI is a relative measure only and since there is toxic substances that increase respiration, to give result in a negative number. The PI of both chemically and biologically synthesized AgN Ps was compared in order to evaluate toxic effect value. The toxic effect value PI of chemically synthesized AgNPs is much greater ( 85.45% ) than the biologically synthesized AgNPs from Garlic ( 46.35% ) . These observation shows that the bacteria Bacillus sub tilis killed...
Jatropha Curcas the Possible Uses, Potential Products and Current Strategies for Biofuel Production in India
Jatropha is well adapted to marginal areas with poor soils and low rainfall, where it grows witho... more Jatropha is well adapted to marginal areas with poor soils and low rainfall, where it grows without competing with annual food crops, thus filling an ecological niche. It is widely distributed in the tropics and is already used to a certain extent. Any further promotion of its use would, therefore, be facilitated by this. The species has numerous uses and in their combination lies the potential of this crop. The most important is the combination of erosion control and oil production. The use of the oil as a substitute for diesel fuel and for soap production in rural areas would improve the living conditions of the people and would offer additional income. As Jatropha is not browsed by cattle, it can grow without protection and can be used as a hedge to protect fields. All parts of the plant are used in traditional medicine and active components are being investigated in scientific trials. Several ingredients appear to have promising applications both in medicine and as a plant prote...
A B S T R A C T The antibacterial activity of extracted biological synthesized sliver nanoparticl... more A B S T R A C T The antibacterial activity of extracted biological synthesized sliver nanoparticles (Bio-AgNPs) were observed 0.027, 0.024, 0.018, 0.028 against B. subtilis and 0.023, 0.020, 0.015, 0.025 against E. coli for garlic, onion, papaya and apple extract respectively. For chemically synthesized sliver nanoparticles (CH-AgNPs) used as reference/control sample, the zone of inhibition was observed 0.033 and 0.029 against pathogens E. coli and B. subtilis. The above observation of antimicrobial activities was clearly indicated that the CH-AgNPs are suitable for inhabit the growth of photogenic bacteria with greater disadvantage of higher toxicity. On the other hand the Bio-AgNPs extract, apple on average equally inhabited the growth of pathogenic bacteria with lesser toxicity then garlic>onion>papaya. Bio-AgNPs was proved under UV-VIS absorption spectroscopy and observed plasmon peak maximum at 399nm, 400nm, 420nm and 410nm for garlic, onion, papaya and apple respectively...
Coronavirus disease (COVID-19) is a respiratory infectious disease caused by a newly discovered v... more Coronavirus disease (COVID-19) is a respiratory infectious disease caused by a newly discovered virus strain, severe acute respiratory syndrome coronavirus-2 (SARS-Cov-2). This pandemic spread quickly across nations with a high mortality rate in immunocompromised patients. This contagious disease posed a serious threat to health systems. It impacted the continents of the earth in a way that could not have been predicted. Therefore, many leading funding agencies announced the call for proposal to diagnosis and treatment of COVID-19 pandemic using advanced technology-based methods, including nanotechnology. The researchers coming from the nanotechnology community can contribute their efforts to cope with COVID-19. As a community member of nanotechnology, we suggest some new research targets that can be designed and improved, optimized, and developed the existing/new materials in the sub-field of diagnostics and healthcare of nanotechnology. The potential research targets to fight agai...
Uploads
Papers by Shruti Tyagi