Cardioprotective Effect of Eugenol Against Cd-Induced Inflammation, Oxidative Stress, and Dyslipidemia in Male Rats: An In Vivo and Molecular Docking Study
Analysis of misinsertion and mispair extension by human immunodeficiency virus type 1 reverse transcriptase (HIV-1RT) in development of drug resistance
Journal of biotechnology & biomaterials, Aug 30, 2016
Evidence-based Complementary and Alternative Medicine, 2013
The demand for interdisciplinary research is increasing in the new millennium to help us understa... more The demand for interdisciplinary research is increasing in the new millennium to help us understand complex problems and find solutions by integrating the knowledge from different disciplines. The present review is an excellent example of this and shows how unique combination of physics, chemistry, and biological techniques can be used for the evaluation of Indian medicinal herbs used for treating diabetes mellitus. Laser-induced breakdown spectroscopy (LIBS) is a sensitive optical technique that is widely used for its simplicity and versatility. This review presents the most recent application of LIBS for detection of glycemic elements in medicinal plants. The characteristics of matrices, object analysis, use of laser system, and analytical performances with respect to Indian herbs are discussed.
British Journal of Applied Science and Technology, Jan 10, 2013
The control of insect pests in agriculture, forestry, stores, animal husbandry, poultry and in hu... more The control of insect pests in agriculture, forestry, stores, animal husbandry, poultry and in human hygiene are still considered a challenge. Widespread use of chemical pesticides represents a potential risk to human and the environment. Therefore, the search for alternative strategies in pest control is timely to overcome this problem. Desirable are preparations that exhibit new modes of actions and impair processes that are rather specific to the pest in order to be combated. In the last twenty five years, much attention has been devoted to natural pest control agents. One of the most important groups among them are plant based active substances or mixtures of substances commonly known as 'botanicals'. Such natural products typically occur as cocktails of metabolically related compounds with differing activity/spectrum towards different insects. The present paper is a mini review presenting an updated account of biopesticidal properties of extracts from two different plant species, that could be developed as a potential substitute to the chemical pesticides.
Journal of biomedical research & environmental sciences, Mar 1, 2023
Journal of Biomedical Research & Environmental Sciences main aim is to enhance the importance of ... more Journal of Biomedical Research & Environmental Sciences main aim is to enhance the importance of science and technology to the scientifi c community and also to provide an equal opportunity to seek and share ideas to all our researchers and scientists without any barriers to develop their career and helping in their development of discovering the world.
Journal of biomedical research & environmental sciences, Jun 1, 2023
The transaminases such as Glutamate Oxaloacetate Transaminase (GOT or AST) and Glutamate Pyruvate... more The transaminases such as Glutamate Oxaloacetate Transaminase (GOT or AST) and Glutamate Pyruvate Transaminase (GPT or ALT) are the key enzymes known for their roles in utilization of protein and carbohydrates. The activities of these two enzymes have been shown to alter in fi sh and other organisms due to pesticide stress. Therefore, the study was initiated to monitor the levels of these enzymes in C. punctatus exposed to 3 subacute concentrations of lindane (0.025, 0.05, 0.1 mg/l) for 96h. The results showed increase in activity of AST and ALT in all the tissue of fi sh at highest concentration; the values being in following order: gills >kidney>brain>heart>muscle>liver for AST and for ALT the values were in following order: br ain>muscles=kidney>gills>liver>heart, after 96hr treatment period. At highest concentration of lindane (0.1 mg/l) tested the maximum rise in AST activity was found in gills (50.84%) and minimum in liver (27.99%). The other organs such as kidney, brain, heart and muscle exhibited 49.42, 45.14, 39.70 and 33.50% rise, respectively. The activity of ALT, however, showed maximum increase in brain (59.60%) and minimum in heart (30.26%) at highest concentration of lindane (0.1 mg/l) tested. The kidney, muscle, gills and liver registered 57.00, 57.02, 52.63 and 38.18% rise, respectively, under this condition.
Misinsertion and mispair extension by human immunodeficiency virus type 1 reverse transcriptase (HIV-1RT) as a mechanism of development of mutations in the viral DNA
Journal of Bacteriology & Parasitology, Jan 18, 2017
Introduction: Natural phytochemicals are considered safe to use as therapeutic agents. There is a... more Introduction: Natural phytochemicals are considered safe to use as therapeutic agents. There is a growing trend toward exploring anticancer effects of crude algal extracts or their active ingredients. Euglena tuba, a microalga, contains excellent antioxidant potential. However, the anticancer property of E. tuba has not been explored. This study investigates the chemical profiling as well as antitumor property of methanolic extract of E. tuba (ETME) against Dalton's lymphoma (DL) cells. Materials and Methods: E. tuba, procured from northern part of India, was extracted in 70% methanol, dried at room temperature, and stored at -20 • C for future use. A freshly prepared aqueous solution of ETME of different concentrations was employed into each experiment. The ETME mediated anti-tumor response in Dalton's lymphoma was evaluated in the inbred populations of BALB/c (H2d) strain of mice of either sex at 8-12 weeks of age. The cytotoxicity of ETME in cancer cells, effects on morphology of cell and nucleus, alteration in the mitochondrial membrane potential, and level of expression of proapoptotic proteins (Bcl-2, cyt C, Bax and p53) were done using known procedures. Results: The ETME contained high content of total alkaloids (96.02 ± 3.30 mg/100 mg), flavonoids (15.77 ± 2.38 mg/100 mg), carbohydrate (12.71 ± 0.59 mg/100 mg), ascorbic acid (12.48 ± 2.59 mg/100 mg), and phenolics (0.94 ± 0.05 mg/100 mg). Gas chromatographymass spectrometry (GC-MS) analysis indicated the presence of 23 phytochemicals with known anticancer properties. DL cells treated with ETME exhibited significant and concentration dependent cytotoxicity. Florescent microscopy and flow cytometry of ETME treated DL cells indicated significant repair in cellular morphology and decreased mitochondrial potential, respectively. Western blot analysis displayed up-regulation of proapoptotic proteins (Bax, Cyt-c, p53) and down regulation of anti-apoptotic protein (Bcl2) in DL cells treated with ETME. Conclusions: The findings of this study clearly indicated that the anticancer property of ETME was mediated via reduction in mitochondrial potential and induction of apoptotic mechanism. Further studies are warranted to explore the anticancer activities of active ingredients present in this microalga of pharmaceutical importance.
Effects of chemical environmental factors (both natural and anthropogenic) on DNA and their modes of action. A. Organic factors (pesticides, dyes) B. Inorganic Factors (Heavy metals)
Pharmacological Chemistry and Biomedical Implications of Chemical Ingredients from Parthenium hysterophorus
Current Topics in Medicinal Chemistry, 2022
: Parthenium hysterophorus L. belonging to the family Asteraceae is a noxious weed in-festation w... more : Parthenium hysterophorus L. belonging to the family Asteraceae is a noxious weed in-festation with allelopathic effects with its lower economic value. It poses a serious risk to its sur-roundings. The presence of oils, polyphenols, flavones, flavonoids, alkaloids, terpenes, pseudoguai-anolides, and histamines in P. hysterophorus makes it important and beneficial due to its medicinal properties. This review article is focused on the history, geographical distribution, chemical compo-sition, and molecular structure of some phytochemicals and ethanopharmacological aspects of P. hysterophorus. The harmful effects of this weed have also been included. The information avail-able from the existing literature revealed that P. hysterophorus is rich in various phytochemicals with different pharmacological activities. However, the complete analysis of different phytoconstit-uents isolated from P. hysterophorus and their specific properties are not fully understood. The spo-radic information published in some journals suggests that this plant could be exploited to develop new drugs against certain diseases, including cancer, HIV-1 infection, and immunological disorders. The structure and mode of action of some compounds such as parthenin and stigmasterol were also discussed. Though the current information on P. hysterophorus indicates the ethnopharmacological implications of extracts of this plant, more systematic and extensive studies are still required to properly understand the contribution of its specific chemical constituents responsible for their vari-ous medicinal properties.
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