Staphylococcus aureus (S. aureus) is an important pathogen affecting children worldwide. Children... more Staphylococcus aureus (S. aureus) is an important pathogen affecting children worldwide. Children are at increased risk of nasal colonization and may be responsible for spreading S. aureus and methicillin-resistant S. aureus (MRSA) to the community settings. This study aimed to determine MRSA nasal colonization among school-going (aged 10–16 years) children of Kathmandu Valley and detect the mecA gene among isolated S. aureus. This study is the first study from Nepal to test the mecA gene from S. aureus from the community (school children). A total of 190 samples were collected from anterior nares, and S. aureus was identified using standard microbiological techniques. An antibiotic susceptibility test was performed, and MRSA screening was done by incorporating the cefoxitin disk in the AST plate. DNA was extracted using the hexadecyltrimethylammonium bromide (CTAB) method, and the mecA gene was detected using PCR. Statistical analysis was carried out using SPSS v16.0. Among the tot...
International Journal of Applied Sciences and Biotechnology, Mar 29, 2022
An insecticidal protein named Vegetative Insecticidal Protein (Vip) produced by Bacillus thuringi... more An insecticidal protein named Vegetative Insecticidal Protein (Vip) produced by Bacillus thuringiensis during its vegetative growth is gaining its popularity as second-generation insecticidal protein because of its lethal effect on insects of class Coleoptera, Lepidoptera, Diptera. The research was designed for the confirmation of Vip3 gene presence in soil samples collected from different geographical region of Nepal. For the purpose, 36 soil samples from different places of Nepal were collected. Primers were used for PCR amplification of DNA templates extracted and product size was observed around 1621bp.Out of total sample analyzed, 10% of sample showed the presence of Vip3 gene.
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Papers by Sandeep Rijal