Metabolism and Nutrition : Phytase and Minerals 124 Effects of temperature , pH , and pepsin on the stability of intrinsic phytase of rye , wheat , and barley
An experiment was carried out to evaluate effects of temperature, pH, and incubation time on the ... more An experiment was carried out to evaluate effects of temperature, pH, and incubation time on the stability of intrinsic phytases. The experiment was conducted as a 3*4*3*3 factorial arrangement with 3 feed ingredients (rye, wheat, and barley), 4 temperatures (T) (20, 38, 55, and 80°C), 3 pH levels (3, 5.5, and 8), and 3 incubation times (30, 60, and 120 min), with 2 replicates per treatment. After incubation at the destined conditions of T and pH, residual phytase activity of treatment groups as well as non-treated (reference) samples were measured. Stability was calculated as the ratio of the residual phytase activity of a treated sample to the reference sample times 100. Phytase activity of the reference samples of rye, wheat, and barley was 3.14, 1.77, and 0.66 FTU/g, respectively. Overall, rye showed the highest and barley the lowest stability (58% vs. 47%). Stability decreased with increasing T to 6.5% at 80°C. Stability of intrinsic phytase significantly decreased with increas...
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