Papers by Muhammad Ansari
Tropical Journal of Pharmaceutical Research, Jun 17, 2016
Purpose: To develop a new spectrophotometric method for the analysis of losartan potassium in pha... more Purpose: To develop a new spectrophotometric method for the analysis of losartan potassium in pharmaceutical formulations by making its complex with copper. Method: A coloured complex based on UV/Vis spectroscopic method was developed for the determination of losartan potassium concentration in pharmaceutical formulations in the visible region. The colored complex of losartan was formed with cupric acetate (5:4). Analysis was carried out by the two methods -absorption ratio and calibration curve methods. The proposed method was validated in terms of linearity, accuracy and precision. Results: The λ maximum of the complex was recorded at 530 nm. Beer`s law was obeyed in the range of 10 -50 µg/ml with a correlation coefficient (r 2 ) of 0.9989. Mean analytical results from the calibration curve and absorption ratio methods were 99.42 and 99.16 % with relative standard deviation (RSD) of 0.97 and 0.82 %, respectively. Mean recovery was between 98.61 and 101.35 % and precision expressed as relative standard deviation (RSD) was 0.91 %. The proposed method is simple, easy to apply, low-cost, and requires relatively inexpensive instruments. Thus, it is a suitable alternative to currently used spectrophotometric methods for the determination of losartan in bulk and solid dosage forms.
Pakistan Journal of Pharmaceutical Research, 2016
In this study four inorganic elements i.e. sodium, potassium, calcium and magnesium were estimate... more In this study four inorganic elements i.e. sodium, potassium, calcium and magnesium were estimated in eleven locally available herbal preparations used as sex stimulants using Flame Atomic Absorption Spectrometry and Flame Emission Spectroscopy. Samples names were Ginseng, Nan-bao, Choe, Safoof-e-Mughallis (Powder), Imsaki (Jawahar) capsules, Zarjam (capsules), Shahi (capsules), Mumsik sultani (Jawahar) capsules, Laboob-e-Kabeer, Laboob Bard (Jawahar wala), Laboob Khas (Jawahar wala). Results indicate the presence of variable amounts of metals in these samples. Order of concentration of metals in different samples has been found to be as Ca> Mg > K > Na. Shahi capsules exhibited highest amount of sodium (3100±1825 µg/g) and other three minerals were in higher quantities. Choe contained highest amount of calcium (54175±4898 µg/g) and magnesium (53223±3205 µg/g) while Mumsik showed highest quantity of potassium (12070±882 µg/g). Ginseng, Nan-bao and Zarjam contained moderate ...
American Journal of Advanced Drug Delivery, 2018
American Journal of Advanced Drug Delivery A m eri can J o u r n a l of Adva n c e d D ru g Deliv... more American Journal of Advanced Drug Delivery A m eri can J o u r n a l of Adva n c e d D ru g Deliv e ry
Pakistan journal of pharmaceutical sciences, 2020
Myelosuppression or bone marrow suppression is one of the most common side effects caused by anti... more Myelosuppression or bone marrow suppression is one of the most common side effects caused by anti-cancer drugs. Certain nonsteroidal anti-inflammatory drugs (NSAIDs), antibiotics and viruses like B19 virus can also cause bone marrow suppression resulting in serious consequences like leukopenia, anemia and thrombocytopenia. Currently, it is mainly treated by Filgrastim, use of which is not without side effects. Certain natural drugs can be a safer alternative to treat myelosuppression. Azadirachta indica, commonly known as Neem, is an important medicinal plant of subcontinent. Keeping in view the traditional uses of Neem, present study aims to investigate its potential role in reversing myelosuppression. Albino rats were used to determine hematopoietic activity of Neem leaves after inducing myelosuppression by cyclophosphamide given subcutaneously. Filgrastim was used as reference standard to compare the antimyelosuppressant activity of the drug. The drug was evaluated in three doses...
Pakistan journal of pharmaceutical sciences, 2020
Numerous ailments have been effectively treated with natural plants for long time all over the wo... more Numerous ailments have been effectively treated with natural plants for long time all over the world. Plants provided a back bone for the exploration of novel medicinal compounds. Therefore, chief focus of our study was to isolate the biologically active compounds from the plant source and evaluate their antidiarrheal potentials, as diarrhea is still the most dominant disease in developing countries. The isolation and structure elucidation of two new compounds were identified from methanolic and chloroform extracts of Psidium guajava (guava) leaves. Extracts of plants were acquired by successive maceration from dried powder. Castor oil induced diarrheal-model was used to evaluate the antidiarrheal activity and therapeutic response was endorsed to the suppression of normal and wet stools in Spraug Dawley rats. Through the series of fractionations, compound-A was obtained from methanolic extract and named 3-(4-amino 1,3,8-tri-OH 5,6-di-CH3 7-propyl 1,2,3,4,4a,5,8,8a-octahydronaphthale...
Targeting folate receptors (α1) to internalize the bleomycin loaded DNA-nanotubes into prostate cancer xenograft CWR22R cells
Journal of Molecular Liquids, 2020
Abstract DNA-nanotechnology based on DNA scaffolding technique is an established approach to form... more Abstract DNA-nanotechnology based on DNA scaffolding technique is an established approach to formulate water-miscible nano-structural frameworks. We have designed the hydrophilic DNA-nanotubes (D-NTs) as a (water-soluble) vehicle to encapsulate a water-insoluble DNA-intercalating anticancer drug. Bleomycin (BM) is a model hydrophobic anticancer drug used in this study capable to bind with the D-NTs to formulate BM loaded D-NTs. This BM@D-NTs system was evaluated for the improvement of the in vitro anti-cancer effects on the resistant prostate cancer xenograft CWR22R cells, over-expressed with the folate receptors/alpha (FRα). D-NTs were functionalized with the FRα-targeting antibodies to interact with the FRα (receptors) highly expressed on the resistant prostate cancer xenograft CWR22R cells. D-NTs not only increased the aqueous miscibility/dispersibility of BM but also enhanced the therapeutic efficiency as a targeted (site-specific) drug delivery system. D-NTs synthesis was achieved by sticky ends cohesions of DNA triangular tiles. DNA triangles were self-assembled from a freshly circularized short scaffold chain (84-NT) by annealing with the various staple strands. The polymerization of the triangular tiles gave rise to DNA-nanosheet lattices which underwent morphological transition guided by the twists in the DNA duplexes. This DNA double helix curvature caused self-coiling of the DNA 2D nano-sheets to condense into D-NTs morphology. Native-Page gel experiment showed decreased electrophoretic mobility down the gel confirming the successful execution of the polymerized lattices via sticky ends cohesion of the DNA-triangles. The final morphology and self-coiling of 2D DNA nano-sheets were confirmed through atomic force microscopy (AFM) showed the successful synthesis of D-NTs having diameter 3 to 5 μm and length 200 to 600 nm. BM was loaded onto D-NTs via incubation of hydro-alcoholic solution of the BM with the aqueous solution of D-NTs followed by the evaporation of alcohol and intercalation of the BM onto D-NTs. The intercalation of BM onto D-NTs was confirmed by UV-shift analysis. The targeted cytotoxicity of the BM@D-NTs for the CWR22R (resistant prostate cancer xenograft) cells was confirmed through MTT assay and the flow cytometry compared to the highly compatible empty D-NTs. Confocal microscopy revealed the time-dependent transfection of BM@D-NTs into CWR22R cells compared to the control cell line.
Journal of Drug Delivery and Therapeutics, 2020
The chief focus of our study is to evaluate the phytochemical and anti-cancer activity of methano... more The chief focus of our study is to evaluate the phytochemical and anti-cancer activity of methanol (PGM) and chloroform extracts (PGC) of the leaves of Psidium guajava (guava) collected from local area of district Sialkot, Pakistan. Shade dried milled leaves was subjected to extraction (maceration) with methanol and chloroform. Quantitative and qualitative screenings by GC-MS and phytochemical techniques were performed. Then different secondary metabolites and phytochemical compounds were identified which are typically associated with the existence of therapeutic characteristics. Psidium guajava has been extensively used as herbal remedies like, anti-diarrheal, antihypertensive, antibacterial, antifungal as well as to control obesity, ulcer, diabetes. In this study, both extracts of P. guajava were evaluated for their anticancer activities against HeLa cell-lines (cancerous cells). The healthiest anticancer response in the form of cell-line suppression was perceived with 200µg/mL of...
ACS Omega, 2019
Multidrug resistance (MDR) is a global threat posed by continuously evolving microbial resistance... more Multidrug resistance (MDR) is a global threat posed by continuously evolving microbial resistance against currently available antimicrobial agents. In this study, we synthesized hydroxyapatite-based porous nanocarriers with pH-dependent biodegradation, using cefazolin (CFZ) as cargo drug against MDR E. coli, S. aureus, and P. aeruginosa. Oval-shaped porous hydroxyapatite nanoparticles (opHANPs) were synthesized via core−shell method. Field emission scanning electron microscopy revealed that the average length and width of opHANPs were found to be ∼90 and ∼110 nm, respectively with monodispersed size and morphology. The encapsulation efficiency (EE) of CFZ was observed to be dependent on the initial concentration of the drug (EE, 41.37−92.40% with 300−2000 μg/mL of CFZ). Brunauer−Emmett− Teller specific surface area and pore width of opHANPs were 166.73 m 2 /g and 3.3 nm, respectively, indicating hierarchal pore distribution. The pH-responsive drug release was observed from CFZ-loaded opHANPs (CFZ@opHANPs). An enhanced drug-releasing behavior was observed at lower pH (4.5, 2.5, and 1.5). The study of release kinetics revealed that at pH 7.4, drug release is due to anomalous diffusion, while at lower pH, the drug release followed fickian diffusion model. Cytotoxic and hemolytic studies showed biocompatibility of CFZ@opHANPs with HepG2 and red blood cells. The growth kinetic study and colony-forming unit assay showed the superior antibacterial potential of CFZ@opHANPs, in contrast to carrier or CFZ alone, against MDR E. coli, S. aureus, and P. aeruginosa strains.
Acta Poloniae Pharmaceutica, May 1, 2014
Artemisinin (ARMN) is a potent antimalarial drug, which is effective against multidrug resistant ... more Artemisinin (ARMN) is a potent antimalarial drug, which is effective against multidrug resistant strains of Plasmodium falciparum and produces rapid recovery even in patients with cerebral malaria. Being poorly soluble in water, artemisinin is incompletely absorbed after oral intake due to poor dissolution characteristics in the intestinal fluids. To enhance these properties, solid dispersions of artemisinin with succinic acid (SUC) were prepared using drug-carrier ratios 1 : 1, 1 : 4, 1 : 6, 1 : 8 and 1 : 10 by solvent evaporation and freeze drying methods. These solid dispersions were characterized by differential scanning calorimetery (DSC), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction patterns (XRD), phase solubility and dissolution kinetics evaluated by applying zero order, first order, Higuchi, and Korsmeyer-Peppas models. Physical mixtures produced significantly higher aqueous solubility and rate of dissolution as compared to artemisinin alone. The dissolution profiles of all formulations followed Higuchi model and exhibited diffusion-controlled release of drug. Solvent evaporation method (SLVPs) exhibited improved solubility and freeze dried solid dispersions (FDSDs) produced highest solubility but stability constant was opposite. ARMN and SUC both were found completely crystalline as shown by their XRD patterns. Physical mixtures (PMs) showed reduced intensity in their XRD patterns while solid dispersions by SLVPs exhibited twice reduced intensity and much displaced angles, whereas FDSDs showed synergistic effects in some of ARMN and SUC peaks. DSC thermograms of FDSDs at drug-carrier ratios 1 : 1ñ1 : 4 showed lower melting temperature and enthalpy change (∆H) values than respective SLVPs, whereas at higher ratios, a reverse was true. SLVPs showed displaced methyl stretching bands at lower drug-carrier ratios and exhibited O-H stretching characteristic bands of SUC at higher drug-carrier ratios. In addition, carbonyl group and CO stretching vibrations characteristic of SUC (1307 cm-1) appeared prominently compared to PMs, whereas CO stretching characteristic bands of ARMN disappeared at higher ratios. FDSDs exhibited distinct nature of bonding compared to respective SLVPs and PMs.
Asian Journal of Chemistry, 2013
The purpose of the present study was to develop a new simple, accurate, precise and economic reve... more The purpose of the present study was to develop a new simple, accurate, precise and economic reverse phase HPLC method for the determination of montelukast sodium in bulk and pharmaceutical tablet dosage form. The separation of analyte was carried on lichorosovol octylsilyl (5 µm 250 mm × 4.6 mm) C8 column and the mobile phase was fixed to acetonitrile and sodium acetate buffer (adjusted to pH 4 with acetic acid), in the proportion of 80:20 v/v, UV detection was carried out at 350 nm with a flow rate of 1 mL/min. The developed method showed that Beer's law was obeyed in range of 0.00008-0.2 mg/mL having correlation coefficient (R 2) of 0.999. The per cent recovery was found from 100-103.28 % which indicate that the method is precise and reproducible. LOD and LOQ of drug were 0.00008 and 0.004 mg/mL, respectively. Precision, specificity, robustness studies showed good repeatability of the applied method. Percentage relative standard deviation values were found less than 2 % for proposed method. It was concluded that proposed method was versatile, low cost, accurate, selective, precise and rapid for the analysis of montelukast sodium.
Chinese Science Bulletin, 2012
Artemisinin (ART) is a sesquiterpene lactone with an endo-peroxide bridge that is thought to be r... more Artemisinin (ART) is a sesquiterpene lactone with an endo-peroxide bridge that is thought to be responsible for its antimalarial activity. It has low oral bioavailability because of aqueous insolubility, which leads to local toxicity at the site of aggregation. The present work focused on increasing its solubility and evaluating its permeation across a model membrane to mimic transdermal delivery that bypasses the hepatic metabolism. For this purpose, physical mixtures (PM), solid dispersions (SD) and lyophilized dispersions (LD) with different drug-polymer ratios (1 : 0.5, 1 : 1, 1 : 2, 1 : 4 and 1 : 9) were prepared using the hydrophilic polymer polyvinylpyrrolidone (PVP). Drug-polymer dispersions were characterized using X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Solubility was measured in three solvents: de-ionized water, phosphate buffered saline (PBS) and methanol. The toluene-water partition coefficient was evaluated and compared with the literature and calculated logP values. In vitro diffusion of ART was studied across a polydimethylsiloxane membrane from a saturated solution of drug-polymer dispersions. XRD patterns showed a gradual decrease in crystallinity of ART with increasing polymer concentration, while FTIR confirmed no interactions between ART and PVP. Solubility was increased up to 4-, 5-and 8-fold for LD in water, PBS and methanol, respectively. The logP for toluene-water was 2.65 ± 0.3, which is in good agreement with literature and calculated logP values. Permeation was enhanced, which is attributed to the decrease in crystallinity and increase in wettability of the drug. The ART flux was significantly higher than that of pure ART (0.12 ± 0.01) with increasing PVP concentration for SD and LD formulations. In conclusion, drug-polymer dispersions with PVP improve the pharmaceutical properties of ART in the order LD>SD>PM.
Development and validation of RP-HPLC method for determination of Famotidine and its application in quality control of different pharmaceutical dosage forms
Journal Chemical Society of Pakistan, 2013
Investigating chaos in ozone layer depletion at Pakistan air space
2009 4th International Conference on Recent Advances in Space Technologies, 2009
Phenomena like, atmospheric pollution, temperature variability, global warming, or ozone layer de... more Phenomena like, atmospheric pollution, temperature variability, global warming, or ozone layer depletion (OLD) may be analyzed for forecasts etc. by solving the primitive dynamic equations along with the continuity and energy ones. The environmental variables actually observed are the consequences of thousand of events, some of which may be poorly defined or imperfectly understood. However, such atmospheric models of meteorological
Dissolution Technologies, 2012
Artemisinin (ART), the parent compound of a novel family of antimalarial drugs, was used as a mod... more Artemisinin (ART), the parent compound of a novel family of antimalarial drugs, was used as a model drug that is lipophilic and has low bioavailability (32%) after oral delivery. The primary objective was to study the effect of polyvinylpyrrolidone (PVP) on the permeation enhancement and solubility of physical mixtures (PM) of ART in PVP-K30 in various solvent systems (i.e., distilled water, phosphate buffered saline [PBS], and methanol). PMs of drug-PVP in 1:0.5, 1:1, 1:2, 1:4, and 1:9 ratios were prepared by simple mixing in a mortar and pestle. Fourier transform infrared (FTIR) spectrophotometry and X-ray powder diffraction (XRPD) were applied to characterize the PMs. The solubility of ART was investigated at 37 ± 0.5 °C in three solvents. Permeation of ART-saturated solutions across a silicone membrane in Franz diffusion cells was studied using Fick's law of diffusion. FTIR and XRPD studies have shown some interactions, and there was a phase change of artemisinin from crystalline to amorphous form as the concentration of PVP-K30 in PM ratios increased. Solubility order increased with an increase in PVP-K30 concentration for the water-PBS solvent system, while in methanol, it was erratic. Flux rate and permeability coefficient were enhanced with an increase in PVP-K30 concentration. In less permeable solvents like water and PBS, the enhancement ratio was high, while the enhancement ratio was low in a highly permeable solvent like methanol. In conclusion, with an increase in concentration of PVP, the permeation rate and solubility of ART increased.
Diastereotopy in some 3,4-dihydroisocoumarins — effect of changing the substituents on the chiral centre
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2000
Diastereotopy has been investigated in eight different derivatives of 3,4-dihydroisocoumarins by ... more Diastereotopy has been investigated in eight different derivatives of 3,4-dihydroisocoumarins by changing the substituent on the pyran ring chiral centre, C-3. A diastereotopic NMR effect is observed for the prochiral methylene protons on the benzopyran ring and the effect is greatly influenced by the pi-electron system of the substituent. The effect is also observed for the substituents, however, the extent of diastereotopy depends upon their nature and the separation of methylene groups from the chiral centre.
Brazilian Journal of Pharmaceutical Sciences, 2011
The purpose of this study was to mask the bitter taste imparted by dihydroartemisinin (DHA) by th... more The purpose of this study was to mask the bitter taste imparted by dihydroartemisinin (DHA) by the use of different coating materials. Trial-1 and trial-2 were conducted to prepare the DHA granules. The granules produced from trial-1 were irregular in shape and smaller in size while the trial-2 granules were more regular and larger in size. The granules obtained from both trials were then coated with two different coating methods, namely A and B, depending upon coating material. The trial-2 granules showed better flow properties than trial-1 granules. In vitro dissolution studies in phosphate buffer at pH 6.8 revealed that granules of trial-2B released only 34% ± 3 DHA in two minutes compared with trial-1A (57% ± 2), trial-1B (48% ± 2) and trial-2A (53% ± 7). The pleasant taste perception (PTP) test also confirmed the taste masking efficacy of trial-2B (P < 0.05). Scanning electron microscopy (SEM) revealed the more regular and smooth surface of trial-2B granules. In addition, th...
Pakistan Journal of Zoology, 2011
A simple and rapid HPLC (High performance liquid chromatography) assay procedure has been develop... more A simple and rapid HPLC (High performance liquid chromatography) assay procedure has been developed and validated for the quantitative determination of metronidazole benzoate for raw material and commercially available suspensions. The HPLC conditions used were, column: C18, (0.5µm particle size, 150×4.6mm); detection: UV at 254 nm; injection volume: 20 µl; mobile phase: methanol and de-ionized water (64:40, v/v); isocratic elution under ambient temperature at flow rate of 1.0 ml min −1 . The procedure separated metronidazole benzoate, in an overall analysis time of about 10 minutes with metronidazole benzoate eluting at about 7.7 minutes. The injection repeatability was 0.05%, the intraday and interday repeatability were 0.59 and 0.91%, respectively. The procedure provided a linear response over the concentration range 10-1000 µgml −1 (r 2 = 1.0000) with the limits of detection and quantitation 0.0158 and 10 µg ml −1 , respectively. The results show no detectable hydrolysis of metr...
Artemisinin (ARMN) is a drug of choice against drug-resistant malaria especially due to Plasmodiu... more Artemisinin (ARMN) is a drug of choice against drug-resistant malaria especially due to Plasmodium falciparum. Being poorly soluble in water, its solid dispersions with nicotinamide (NA) were prepared at various drug-carrier ratios (1:1, 1:4, 1:6, 1:8, 1:10) by solvent evaporation and freeze drying methods. These solid dispersions were characterized by differential scanning calorimetery (DSC), fourier transform infrared spectroscopy (FTIR), X-ray diffraction patterns (XRD), phase solubility and dissolution studies. Artemisinin and nicotinamide both were found completely crystalline as shown by their XRD patterns. Physical mixtures (PMs) showed decreased intensity in their XRD patterns while solid dispersions by solvent evaporation method (SLVPs) exhibited displaced angles and decreased intensity whereas freeze dried solid dispersions (FDSDs) showed least number of peaks having low intensity and maximum displaced angles. DSC thermograms of drug-carrier ratios at 1:1-1:4 showed lower ...
Spectrophotometric method for the determination of Gemifloxacin mesylate in pure and tablet dosage form
Pakistan Journal of Pharmaceutical Sciences, Sep 1, 2014
Evaluation of physicochemical parameters of selected brands of pharmaceutical oils sold in Punjab, Pakistan
LATIN AMERICAN JOURNAL OF PHARMACY
Twenty four samples of selected brands (local and imported) of pharmaceutical oils (almond, cod l... more Twenty four samples of selected brands (local and imported) of pharmaceutical oils (almond, cod liver, clove, mustard, olive, and sesame oil) sold in Punjab, Pakistan, were investigated using standard procedures of the Association of Official Analytical Chemists (AOAC), the physicochemical properties (color, refractive index, viscosity, acid value, peroxide value, and iodine value) were determined. All oil samples collected from local market (series 3 & 4) showed refractive indices, viscosities, and acid values higher than smaller packing imported and pharmaceutical brands of oils (series 1 & 2 respectively). The results showed that the clove oil sample no. Cl-2 has unacceptable higher acid values and unusual iodine value while mustard oil sample no. M-4 exhibited unacceptable higher acid values. Saponification values of all samples were within international Codex standards. Almond oil sample no. A-4 and olive oil sample no. O-4 had higher peroxide values (> 10.0 mg/g of oil) whi...
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Papers by Muhammad Ansari