Papers by Mst. Afifa Khatun
Microplastics in Different Fish and Shellfish Species in the Mangrove Estuary of Bangladesh and Evaluation of Human Exposure
SSRN Electronic Journal
Potentiality of ginger extract as natural preservative for raw tilapia fish ( Oreochromis mossambicus ) during storage at refrigerated temperature
Journal of Food Processing and Preservation

Archives of Applied Science Research, 2016
In this work, superwater absorbent hydrogel (SWA) based on polysaccharide was prepared from aqueo... more In this work, superwater absorbent hydrogel (SWA) based on polysaccharide was prepared from aqueous solution of carboxymethyl cellulose (CMC)/acrylamide with radiation processing technology using Co-60 gamma source at room temperature (~ 27ÂoC). When SWA is mixed with soil, the morphological study i.e., plant height, weight of roots, number of branch, number of leaves and yield of fruits of tomato plant showed higher values than that of the soil without SWA. It has also been observed that with and without SWA the elemental properties of soil gave almost the same values meaning that adding SWA did not affect the properties of soil. Regarding the quality of fruit, the food value (i. e., protein, ascorbic acid, iron) of green tomato showedno significant changes with and without SWA, although the significant increase in total phenol content of green tomato grown in SWA mixed soil indicates better antioxidative quality.
Environmental Nanotechnology, Monitoring & Management, 2019
Highlights Elemental abundances of soil & water samples from a southern part of Bangladesh are ... more Highlights Elemental abundances of soil & water samples from a southern part of Bangladesh are presented. Natural radioactivity of soil & physicochemical parameters of water samples are reported. Base-line data for our studied pre-industrial area (Rampal) has been established. Statistical approaches have been used to assess the measured analytical data. Using environmental and radiological indices environmental geochemistry has been evaluated.
Gamma Radiation Processing of Honey of Mustard, Black Seed and Lychee Flower: Measurement of Antioxidant, Antimicrobial, and Fourier Transform Infrared (FT-IR) Spectra
SSRN Electronic Journal, 2021

Japan Agricultural Research Quarterly: JARQ, 2022
We evaluated the effects of processing (i.e., polishing, washing, boiling, and rice cake preparat... more We evaluated the effects of processing (i.e., polishing, washing, boiling, and rice cake preparation) on the stable carbon (δ 13 C), nitrogen (δ 15 N), and oxygen (δ 18 O) isotopic compositions of rice (Oryza sativa) to assess its geographical origin. There were no significant differences in their δ 13 C, δ 15 N, and δ 18 O values before and after boiling and rice cake preparation, indicating that the database of the δ 13 C, δ 15 N, and δ 18 O values of rice samples could be adapted and used to trace the geographical origin of rice used for cooking and rice cake preparation. Conversely, the δ 13 C and δ 18 O values were significantly altered after polishing. After the brown rice samples were polished at a polishing rate of 90%-92%, we determined the δ 13 C, δ 15 N, and δ 18 O values of the polished rice samples from nine countries-Australia,

Remedial role of exercise training to deep‐fried oil‐induced metabolic and histological changes in Wistar rats
Journal of Food Biochemistry, 2020
Exercise training is a well-known lifestyle to maintain good health. The present study was conduc... more Exercise training is a well-known lifestyle to maintain good health. The present study was conducted to explore the effect of regular exercise training (for 15 min) on biochemical, physiological, and histological changes in fried oil intake (5 g/kg body weight, for 5 weeks) with or without raw oil supplementation. Liver disease and heart muscle injury were accounted for by significant (p < .05) increase in liver and heart biomarker enzymes in serum. Creatinine and urea level were also significantly increased in the fried oil-fed group as a sign of kidney injury. But all the biological markers including triglycerides, low-density lipoprotein cholesterol (LDL-C) was decreased significantly (p < .05) in the exercise-trained rat group. These metabolic changes were substantiated by the histological study of respective organs. Therefore, people should avoid repeatedly deep-fried oil consumption rather than fresh ones though regular exercise training has been found to resolve these metabolic abnormalities. PRACTICAL APPLICATIONS: Exercise training is a common practice to reduce the onset of some metabolic abnormalities for example cardiovascular disease, non-alcoholic fatty liver disease, chronic kidney disease, and also prevents various cell and tissue damages. Repeated frying of vegetable oil not only reduces its nutritional value but also produce free fatty acids and other toxic compounds. The present study revealed that repeatedly heated vegetable oil consumption causes injuries in the heart, liver, kidney, and small intestine by the oxidative products. As a result, the level of biomarkers of these responsive organs was found to be elevated. But regular exercise training ameliorated these detrimental effects. So, in terms of public health concern, regular exercise is one of the best ways to keep the body fit especially those who are consuming deep-fried oil.
Gamma radiation processing of honey of Mustard, Black seed and Lychee flower: Measurement of antioxidant, antimicrobial, and Fourier transform infrared (FT-IR) spectra
Measurement: Food, 2022
Biochemical composition and effects of radiation on sensory, biochemical and physiological qualit... more Biochemical composition and effects of radiation on sensory, biochemical and physiological quality of fresh spinach (Spinacia oleracea L.)

Bitter gourd (Momordica charantia L.) is one of the most popular vegetables in some Asian countri... more Bitter gourd (Momordica charantia L.) is one of the most popular vegetables in some Asian countries for their antioxidant properties and bioactive compounds to lower of diabetic diseases. The Objective of this study was to investigate the effect of gamma radiation on the antioxidant marker compounds (total phenol content, flavonoid content and ascorbic acid content) of fresh bitter gaurd. Microbial safety (total bacterial count, total coliform count, and total Salmonella count) was also examined. Phenolic content was significantly (p < 0.05) higher in bitter gaurd irradiated with 0.25 kGy compared to the control. Increased flavonoid content was observed in irradiated sample (0.25 and 0.5 kGy) compared to control sample. Ascorbic acid content decreased significantly with the increase of irradiation doses. Gamma irradiation (0.25 and 0.50 kGy) significantly reduced the microbial count in bitter gourd to keep the samples microbiologically safe.

Food Chemistry: Molecular Sciences, 2021
Food origin authentication is imperative for consumer protection. This study explored meat and mi... more Food origin authentication is imperative for consumer protection. This study explored meat and milk species identification as a pioneering country-specific report on mislabeling prevalence among processed meat and milk products in Bangladesh. Meat products (64; sausages, burger patty, meatball, kabab) labeled as chicken or beef and Mozzarella-type cheeses (25), made in Bangladesh and of overseas origins, were analyzed for species detection. Two duplex PCRs (cattle-buffalo and chicken-pig) were applied with species-specific mitochondrial Cyt b gene primers and found to be accurate for species identification in meat and milk products. Bangladeshi origin beef-labeled products were found to be mixed with buffalo (n = 2) and chicken (n = 5) suggesting up to one third of products might be mislabeled. Such mislabeling would allow these 'beef' products to charge a higher price compared with chicken products that command a lower price. Imported meat products were also found mislabeled with buffalo and chicken. Cheese samples, declared as bovine, were found to contain buffalo DNA, and no cattle or buffalo DNA was found in six imported cheese samples. All the meat and cheese products were Halal, as none contained pig DNA. This pilot study shows the majority of products were labelled correctly, but large proportions were not and strict monitoring is recommended to ensure food safety and address consumer preferences, especially religious concerns.
International Journal of Environment, Agriculture and Biotechnology, 2018
Milk consumption rendering the proof of geographical provenance is a vital issue in food and cons... more Milk consumption rendering the proof of geographical provenance is a vital issue in food and consumer protection. The present study deals with discrimination of Bangladeshi cow milk according to their region of production using multi element compositions.

Abstract: Antioxidant property of Sasa senanensis Rehd
ISSN 2168-8761 print/ISSN 2168-877X online ~ 236 ~ http://www.researchpub.org/journal/jbpr/jbpr.h... more ISSN 2168-8761 print/ISSN 2168-877X online ~ 236 ~ http://www.researchpub.org/journal/jbpr/jbpr.html Abstract: Antioxidant property of Sasa senanensis Rehd (Kumaisasa) leaf powder was examined both in vitro and in vivo. Kumaisasa is a native Japanese dwarf bamboo plant and the leaves have been traditionally used as tea, functional food, or folk medicine, such as for wrapping sushi and chimaki (rice cake) to prevent putrefaction. When the antioxidant activities were determined by calorimetric and Electron Spin Resonance (ESR) assays for the aqueous and 80% methanol (MeOH) extracts, the DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging activity was not markedly different between the aqueous and MeOH extract but the Oxygen Radical Absorbance Capacity (ORAC) value of the MeOH extract showed almost twice as strong as aqueous extract. Hydroxyl radical and superoxide radical scavenging activities were also higher than in MeOH than aqueous extract. Consistently, both the content of fl...

Study on antibacterial and antioxidant activities of raw and fermented Moringa Oleifera lam. leaves
Journal of Microbiology and Biotechnology Research, 2017
The Moringa plant (Moringa oleifera Lam.) has been the object of much research due to its multipl... more The Moringa plant (Moringa oleifera Lam.) has been the object of much research due to its multiple uses and wellknown bactericidal potential with good nutritional and therapeutic values. Raw (non-fermented) and fermented leaves were evaluated for antibacterial and antioxidant properties. The present study revealed that fermented leaves have great antibacterial activities against pathogenic microbes such as Pseudomonas aeruginosa, Staphylococcus aureus, Proteus vulgaris, Alcaligens spp., Micrococcus spp., Shigella spp., as well as two KTCC cultures of Bacillus cereus. The fermented leaves showed highest inhibition (16mm) to Proteus spp. and lowest inhibition (7 mm) to Pseudomonas spp. This result suggests that the fermented leaves are superior to non-fermented dried leaves considering antibacterial activities. Although, in comparison with raw leaves, fermented leaves showed reduced phenol content and DPPH radical scavenging activity. Keyword: Moringa oleifera Lam leaf, Sauerkraut pro...

Microbiological Quality Improvement of Dried Fish by Gamma Irradiation and Assessment of Food Value Upon Irradiation with Respect to Biochemical Aspect
A study was conducted to determine the effects of radiation on biochemical (moisture, protein, fa... more A study was conducted to determine the effects of radiation on biochemical (moisture, protein, fat, ash, carbohydrate, calcium and phosphorus) and microbiological qualities (TVBC, TCC and TFC) of three different dried fishes (Chepa, Loitta and Chingri) collected from retails markets. Among the three non-irradiated dried fishes, the highest moisture content (40.85 ± 0.42) was found in Chepa compare to Loitta (19.48 ±0.40) and Chingri (17.09 ±0.77). Highest rate of total protein content was found in non-irradiated Chingri (60.39 ±0.75) and Loitta (55.85 ± 0.40) compare to Chepa (32.67 ± 0.06). Results revealed that radiation (3 and 5 kGy) has no significant effect on biochemical composition of three dried fishes. Results of microbiology showed that total viable bacterial count (TVBC) was estimated in non-irradiated Chepa (5.58 ± 0.14 log cfu/g), Loitta (3.72 ± 0.09 log cfu/g) and Chingri (5.34 ± 0.15 log cfu/g) respectively which was significantly reduced by 1 to 3 logs at 3 kGy and 3...

American Journal of Food and Nutrition, 2013
Under surface culture condition, a number of cheap indigenous raw materials like cane molasses an... more Under surface culture condition, a number of cheap indigenous raw materials like cane molasses and jackfruits were selected for citric acid fermentation and gamma ray induced mutant strains of 14/20 and 79/20 of Aspergillus niger were used. The rate of citric acid production was also influenced by biomass production and residual sugar used. In both cases on the day 13 without presence of Prescott salt for the mutant strain of Aspergillus niger 14/20 the citric acid production was highest. The biomass production was found different in various fermentation medium. It was found to increase with the increase of fermentation period and maximum biomass was found on day 13. It is also found that biomass production was higher in mutant strain of A. niger 14/20 than 79/20. In addition to this, wet-weight biomass was higher than dry weight biomass. Without presence of Prescott salt, wet weight of mycelial body of the fungus Aspergillus niger 14/20 was found highest on day 13 in mixed substrat...

International Journal of Fisheries and Aquatic Studies, 2019
This research work has been conducted to evaluate the effect of gamma radiation and ascorbic acid... more This research work has been conducted to evaluate the effect of gamma radiation and ascorbic acid on organoleptic, biochemical and microbial quality to extend shelf life of freshwater hilsa. Organoleptic evaluation showed that ascorbic acid (1% and 2%) treated and irradiated (1.5 and 3 kGy) samples remained acceptable up to 19 and 21 days, respectively of storage periods at 4 oC. Tyrosine and phenolic content of fish flesh increased with the increase of storage periods in all the samples but increment of tyrosine value was somewhat lower in irradiated sample compare to control. However, the increasing trend of thiobarbituric acid reactive substance (TBARS) value of fish flesh was higher in both treated samples compared than control. Microbial assessment showed the better result in irradiated (3 kGy) sample compared to other and remained within the safe limit. The present study demonstrates that irradiation is effective for preservation of hilsa at 4oC temperature. Though, radiation ...

Hilsa is the only fish that has secured recognition as the geographical indication product of Ban... more Hilsa is the only fish that has secured recognition as the geographical indication product of Bangladesh. The present study was carried out to compare the morphometric, meristic and proximate composition of freshwater and marine hilsa fish. A number of freshwater hilsa were collected from Sandhya river and marine hilsa were collected from Chittagong Port. Morphometric characteristics showed higher value in marine hilsa than freshwater. Among them, total length (TL), standard length (SL) and head length (HL) of both varieties showed difference. Body proportions of both hilsa fish, were different. Number of pectorals, pelvic, anal fin rays showed different. Proximate data showed that, freshwater hilsa contained higher moisture content (70 ± 0.34 %) than marine hilsa. Protein (20.06 ± 0.94 %), lipid (13.8 ± 2 %) and ash contents (1.26 ± 0.15 %) were relatively higher in marine hilsa. Freshwater hilsa showed large amount of calcium (182.4 ± 3.48 mg/100 g) and phosphorus (133 ± 4.52 mg/ ...
1 Food Technology Division, Institute of Food and Radiation Biology, Atomic Energy Research Estab... more 1 Food Technology Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, GPO Box-3787, Savar, Dhaka-1000, Bangladesh 2 Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka, Bangladesh 3 International Affairs Division, Bangladesh Atomic Energy Commission, Paramanu Bhaban, Dhaka-1207, Bangladesh 4 Department of Biochemistry and Molecular Biology, Gono Bishwabidyalay, Savar, Dhaka, Bangladesh

Current Research in Food Science, 2021
This study was conducted with a popular, low price Bangladesh rice variety BRRI Dhan 29 with a vi... more This study was conducted with a popular, low price Bangladesh rice variety BRRI Dhan 29 with a view to possible carbohydrate modification targeting lower glycemic index using gamma radiation application. Irradiation process (5 and 10 kGy at a dose rate of 9.74kGy/h) altered amylose content, amylose/amylopectin ratio, swelling power, and solubility index. FTIR spectroscopy confirmed the changed molecular structures due to radiation application. Treatment with a dose of 5 and 10kGy caused increased amylose and amylose/amylopectin ratio significantly (p < 0.02). The highest amylose content was found in 10 kGy irradiated rice (30.20%) compared to unirradiated one (17.27%). Gamma radiation caused decreased swelling and increased water solubility in rice powder. These features of carbohydrate amendment in irradiated rice lead to reduced glycemic index as investigated with the in vivo experiments. Therefore, this study suggests gamma-irradiated rice (10kGy) is beneficial for diabetic subjects keeping lower blood glucose levels.
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Papers by Mst. Afifa Khatun