Papers by Mohammed Talha Khan
PubMed, 2011
β-thalassaemia, an autosomal recessive hemoglobinopathy, is one of the commonest genetically tran... more β-thalassaemia, an autosomal recessive hemoglobinopathy, is one of the commonest genetically transmitted disorders throughout the world. Collective measures including carrier identification, genetic counseling and prenatal diagnosis are required for preventing β-thalassemia. To achieve this objective, Identification of the spectrum of genetic mutations, especially for various ethnic backgrounds in Pakistan is necessary. Therefore, we designed a cross sectional prospective study to identify the frequency of various gene mutations in different ethnic groups of Pakistan. Over a 5-year period, DNA from 648 blood samples [including specimens of chorionic villus sampling (CVS)] were analyzed for the twelve most common β-thalassemia mutations found in the Pakistani population by a Multiplex amplification refractory mutation system (ARMS). The most common mutation identified was Intervening Sequence 1-5 (IVS 1-5 (G-C)); accounting for 40.89% mutated alleles, and was represented in all ethnic groups. 15.7 % of the β-thalassemia alleles were found to have Frameshift 8-9 (Fr 8-9) as the second most common mutation Other common genetic defects responsible for β-thalassemia: IVS 1-1 (G-T) was found in 8.17%, Codon-30 (Cd-30 (G-C)) 8.02%, Codon-5(Cd-5 (-CT)) contributed 2.16% and Deletion 619 base pair (Del 619bp) affected 11.11% were found in Pakistan. This large study adds to the pre-existing data in Pakistan. Knowledge of the predominant mutation in a given ethnic group will not only help in developing a short panel of (population-specific) primers of mutations thereby providing a cost-effective method for prenatal diagnosis and also help the clinicians to counsel regarding blood transfusion regimen/ pregnancy termination.
Pattern of injuries from road traffic accidents at tertiary care teaching hospital
Medpulse International Journal of Forensic Medicine, 2020
Study of Ossification at Elbow Joint in School Boys Aged 13–16 Years in Hyderabad City
Indian journal of forensic medicine and toxicology, 2017
Age estimation of living is one of the most important tasks faced by Forensic practitioners, espe... more Age estimation of living is one of the most important tasks faced by Forensic practitioners, especially in developing countries like India. A study was conducted to know the status of ossification at Elbow joint in school boys of 13–16 years in Hyderabad city.
Determination of Age by Pubic Symphysis Metamorphosis in Hyderabad Population
Medico-Legal Update, 2016
Forensic medical specialists are very often faced with the problem of fixing the individuality of... more Forensic medical specialists are very often faced with the problem of fixing the individuality of unknown human bodies. The bones are relatively indestructible structures of the body. Bones have abundance of information in them as they resist putrefaction to a great extent. Age related changes occurring in the pubic symphysis in 112 bones (60 males and 52 females) have been explored in this study using Mckern-Stewart criteria. The results show that the Mckern-Stewart criterion can be equally useful in estimation of age for Indian population also.
Profile of Fatal Electrocution in Visakhapatnam Region
Indian journal of forensic medicine and toxicology, 2013
It is a prospective study of fatal electrocution cases brought for post mortem examination at the... more It is a prospective study of fatal electrocution cases brought for post mortem examination at the Department of Forensic Medicine & Toxicology, Andhra Medical College, Visakhapatnam, Andhra Pradesh. Fatal electrocution cases accounted 1.68% of all total cases of autopsies done at our centre during the study period. Majority of the victims were male belonging to the age group of 21–30 years. Incidence was more during the summer and monsoon seasons. Most of the victims were from the unskilled or skilled category. Majority of the cases were accidental in nature and spot death.
To Study the Association between Weather and Frequency of Cataract Surgeries Performed Over Three Years in four different hospitals of Lahore, Punjab
Pakistan Journal of Medical and Health Sciences, Jun 30, 2021
Aim: To study the association between weather and frequency of cataract surgeries performed over ... more Aim: To study the association between weather and frequency of cataract surgeries performed over the year in four different hospitals of Lahore, Punjab. Place and duration of study: The data of 8375 patients who underwent cataract surgeries over last three years 2017, 2018 and 2019 from four different hospitals, Medicare Eye hospital, Jinnah Hospital, Akhtar Saeed Trust Hospital and Lahore General Hospital, was collected. Study design: It was a cross sectional survey. Non-probability conventional sample was used. The data was analyzed by SPSS-20 and presented in forms of bar charts and line graphs. Methods: Total 8375 patients were selected from previous records for our study from four different centers. The hospitals were Akhtar Saeed trust hospital, Jinnah hospital,Lahore general hospital and Lahore Medicare Eye center. Lahore General hospital (LGH) and Jinnah Hospital are from government sector. Akhtar Saeed Hospital is a trust hospital and Lahore Medicare is a private Hospital.All patients with unilateral or bilateral cataract with age above 40 years were included where as patients under 40 years, cataract planned under general anesthesia and patients who had cataract associated with retinal attachment were excluded. Results: Total patients included in our study were 8375.There were 4439 (53%) men and 3936 (47%) women. Out of these 3721 (44.43%) from Jinnah hospital, 2498 (29.83%) were from Lahore General Hospital, 1676 (20.01%) from Medicare and 480 (5.73%) from trust Hospital. In Jinnah hospital, the highest number of cataract surgeries were observed in months of March 500 (13.44%), October 375 (10.08%) and February 341 (9.16%) and months with a smaller number of cataract surgeries were July 201 (5.40%), June 185 (4.9%) and August 170(4.57%).In LGH three months showing maximum number of patients were October313(12.53%), November 285(11.41%) and April 281(11.25%) and minimal in July 158(6.33%), August 128 (5.12%) and June 99(3.96%). Conclusion: It was clear from results of our study that higher number of cases were seen in months of March, April and October and the lowest number of surgeries were observed in months of June, July and August on an average. A definite seasonal variation was observed in number of cataract surgeries performed over the year, in different eye hospitals of Lahore (Punjab) Pakistan. Keywords: Cataract, Surgery, Seasonal variation
Phase 1 Clinical Trial of Prion-Filtered Red Cell Concentrates (pfRCC) in Patients Requiring Allogeneic Blood Transfusion
Blood, Nov 16, 2008
Background; Transmission of vCJD by blood transfusion from pre-symptomatic blood donors has occur... more Background; Transmission of vCJD by blood transfusion from pre-symptomatic blood donors has occurred in 4 reported cases to date. Screening blood donors for infectivity is unlikely to be feasible for several years. Removing infectivity from blood using selective filtration may provide a useful degree of protection from transfusion transmission of the disease. A filter that may remove infectivity from red cell concentrates has been developed and trialed in volunteers receiving autologous blood. No studies have been carried out to date of pfRCC in allogeneic transfusions in the clinical setting. Aims; To establish safety and tolerability of transfusion of prion filtered red cell concentrates. Methods; Twenty patients scheduled to receive transfusion were recruited following ethical approval and with informed consent. Prion filtered units were prepared by the Irish Blood Transfusion Service. A mean loss of 9 gm of haemoglobin per unit of RCC occurred during the filtration process. Each patient received one unit of pfRCC, and a median of 2 units overall (range 1 to 4 units) per transfusion episode. A cross-match sample, full blood count (FBC), renal and liver profile was taken from each patient prior to transfusion. Patients were observed for adverse reactions. After 24 hours, FBC, renal and liver profile were repeated. Six weeks after the transfusion a further sample was tested for red cell antibodies. Six of these patients have consented to undergo re-transfusion with pfRCC. Two re-infusions have taken place uneventfully six months after the first exposure to pfRCC and 4 more are planned. Results No serious adverse events were encountered during the study, or at 24 hour and 6 week follow up after the initial transfusion episode. Mean haemoglobin increment per unit transfused was 0.68g (SD 0.45g; range −0.5 to 1.35g ). Recruitment and follow-up is ongoing in patients exposed to repeat transfusion challenge. Summary The first clinical transfusions of pfRCC were well tolerated. Two patients were rechallenged with transfusions of pfRCC without adverse effect. Further studies with transfusions of prion filtered red cells are now warranted to extend the safety data and to determine whether efficacy is comparable to standard transfusions in adult and paediatric populations.
Phytochemical and biological studies of Tagetes erecta and its clinical evaluation in the treatment of hallux abducto valgus and its associated condition, bunion
Doctoral thesis, UCL (University College London)., 1999
Predictive Control of Energy Management System for Fuel Cell Assisted Photo Voltaic Hybrid Power System
Advances in Intelligent Systems and Computing, 2018
Distributed generation systems also known as hybrid power systems which involve renewable energy ... more Distributed generation systems also known as hybrid power systems which involve renewable energy sources are extensively used due to their efficiency and green interface. Considering the varying environmental conditions, these systems are prone to many disadvantages and limitations. In order to overcome these constraints, intelligent techniques which can achieve steady process and power balance are to be implemented. This paper provides an intelligent control using fuzzy inference system and energy management algorithm for Fuel cell assisted PV Battery system. The supervisory control was implemented to achieve utmost feasible efficiency despite varying conditions such as irradiance and Hydrogen levels. With Levelized cost being adapted, an efficient energy management system attributes for even power distribution throughout the day can be implemented. Our thought process was demonstrated, and final software interface was simulated using MATLAB/Simulink to obtain results which confirm...
Energies, 2021
The use of artificial intelligence (AI) is increasing in various sectors of photovoltaic (PV) sys... more The use of artificial intelligence (AI) is increasing in various sectors of photovoltaic (PV) systems, due to the increasing computational power, tools and data generation. The currently employed methods for various functions of the solar PV industry related to design, forecasting, control, and maintenance have been found to deliver relatively inaccurate results. Further, the use of AI to perform these tasks achieved a higher degree of accuracy and precision and is now a highly interesting topic. In this context, this paper aims to investigate how AI techniques impact the PV value chain. The investigation consists of mapping the currently available AI technologies, identifying possible future uses of AI, and also quantifying their advantages and disadvantages in regard to the conventional mechanisms.
Study of acute poisoning cases at tertiary care teaching hospital
MedPulse International Journal of Forensic Medicine, 2020
Genotype of Beta Thalassemia Patients As Predictors for Prognosis Management without Blood Transfusion
Blood, 2011
3199 Introduction: Packed red blood cell (PRC) transfusion with iron chelation, despite their und... more 3199 Introduction: Packed red blood cell (PRC) transfusion with iron chelation, despite their undesirable effects, has been the mainstay of treatment for patients with beta-thalassaemia major. In the recent past introduction of oral medications that augment Hemoglobin F (HbF) has opened new horizons in the management…
Genetic Modifiers Of Beta-Thalassaemia Associated With Management Without Blood Transfusion: Ten Years Experience
Blood, 2013
Background Long survival with improve quality life in treatment of thalassaemia patients can be p... more Background Long survival with improve quality life in treatment of thalassaemia patients can be procure by blood transfusion and sedulous chelation but, it is cumbrous. Therapeutic manoeuvers designed to stabilize or increase hemoglobin without transfusion and to reduce the blood transfusion. Efforts have been undertaken to find an alternative approach to transfusion in β-thalassaemia. Methods On the basis of our previous studies evaluating the safety & efficacy of Hydroxyurea (HU) in beta thalassemia patients which is an oral Hb-F augmentation agent, at 10-15 mg/kg/day was used on 473 patients for Mean duration of 3 years under the guidelines of Helsinki’s declaration. Response was measured by using transfusion requirement prior to 6 months period of enrollment in the study as control. Patients were finally divided into Complete Responders (CR), partial responders (PR) and non-responders(NR).Patients’ genetic profile was investigated. Chi square test was applied to check associatio...
International Journal of Power Electronics and Drive Systems (IJPEDS), 2018
The present research shows the effect of dust accumulation on the surface of photovoltaic (PV) mo... more The present research shows the effect of dust accumulation on the surface of photovoltaic (PV) modules, which cause losses in their output power. We got 28% of losses in output power at Madinah city during 60 days of dust accumulation. Two ways were used to study the effect of dust on the PV modules of type monocrystalline silicon: the quantitative and the qualitative approaches respectively. A model based on dust density is used to determine the losses of output PV power. We propose to add an important parameter noted dust accumulation coefficient (%/mg.cm-2), in data sheet of PV modules manufacturer. In addition, an intelligent cleaning system is proposed, using the notion of dust density, to start cleaning when an admissible value of power losses is reached. This process allows minimizing the effect of dust.
Histopathological Study of Hepatorenopulmonary Changes in Deaths due to Organophosphorus Poisoning
Indian Journal of Forensic Medicine & Toxicology, 2017
Postmortem changes in these detected cases are usually nil or in few cases late changes due to po... more Postmortem changes in these detected cases are usually nil or in few cases late changes due to poison may be seen. Present study of histopathological changes in liver, lung and kidney in Organophosphorus poisoning is in relation to survival time after the consumption of poison along with that a suggestion that can have been proposed for quick diagnosis and early management of poisoning death. To study the effects of organophosphate on human body with particular reference to liver, lungs and kidney. The histopathological examination can support in arriving at the cause of death due to poisoning while toxicological analysis report is awaited. This can cut short the legal proceedings which is used to remain pending for evidence of poison from chemical analysis. Even though the histopathological findings are not specific for organophosphorus poisoning the progressive changes helps in the estimation of survival period after ingestion of poison and there by time since death before prior to autopsy.
Malaysian Journal of Medical Sciences, 2017
Neurofibromatosis-1 (Non-FSNF1) have been described in literature with tumors involving the spina... more Neurofibromatosis-1 (Non-FSNF1) have been described in literature with tumors involving the spinal roots at every level being even rarer. We reported an interesting case of bilateral symmetrical cervical neurofibroma with multiple spinal neurofibromas appearing as mirror image on CT, associated with non familial NF-1 as a rare presentation in a 25-year-old adult male.
Electrical energy transfer assembly for a motorcycle wheel
Audit of referral of diabetic patients
Saudi Medical Journal, Feb 1, 2002
To determine the referral, the feedback rates and to find out the prevalence of diabetic retinopa... more To determine the referral, the feedback rates and to find out the prevalence of diabetic retinopathy among diabetics who were referred from a primary health care center, to Abha General Hospital in Aseer Region. This study was conducted at Wasat Abha Primary Health Care Center in Aseer region at the end of 1996 and 1997. All the diabetic files were reviewed and evaluated for referral to the eye clinic, their feedback and the prevalence of diabetic retinopathy among diabetics for whom fundoscopy was carried out. The related data was entered and analyzed by using the statistical package for social sciences program. A total of 203 diabetic files were reviewed. Forty percent were referred during 1996 while 68.5% were referred during 1997. The rates of feedback were 72% in 1996 and 71.2% in 1997. Twenty percent were referred twice during these 2 years. The prevalence of diabetic retinopathy was 11.3%. The referral rate and the feedback from the eye clinic was lower than the national target. A poor referral system was responsible for such a low rate. Either reorganization of the referral system or calling the ophthalmologists to attend Primary Health Care Centers monthly will increase the rate of eye examination among diabetics and will help in early detection of diabetic retinopathy and in prevention of blindness.
The problem of constructing optimum stratum boundaries (OSB) and the problem of determining sampl... more The problem of constructing optimum stratum boundaries (OSB) and the problem of determining sample allocation to different strata are well known in the sampling literature. To increase the efficiency in the estimates of population parameters these problems must be addressed by the sampler while using stratified sampling. There were several methods available to determine the OSB when the frequency distribution of the study (or its related) variable is known. Whereas, the problem of determining optimum allocation was addressed in the literature mostly as a separate problem assuming that the strata are already formed and the stratum variances are known. However, many of these attempts have been made with an unrealistic assumption that the frequency distribution and the stratum variances of the target variable are known prior to conducting the survey. Moreover, as both the problems are not addressed simultaneously, the OSB and the sample allocation so obtained may not be feasible or may be far from optimum. In this paper, the problems of finding the OSB and the optimum allocation are discussed simultaneously when the population mean of the study variable y is of interest and its frequency distribution ( ) f y or the frequency distribution ( ) f x of its auxiliary variable x is available. The problem is formulated as a Nonlinear Programming Problem (NLPP) that seeks minimization of the variance of the estimated population parameter of the target variable, which is subjected to a fixed total sample size. The formulated NLPP is then solved by executing a program coded in a user's friendly software, LINGO. Two numerical examples, when the study variable or its auxiliary variable has respectively a uniform and a righttriangular distribution in the population, are presented to demonstrate the practical application of the proposed method and its computational details. The proposed technique can easily be applied to other frequency distributions.
Non Antibiotic Selectable Markers for Live Vaccines
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Papers by Mohammed Talha Khan