Papers by Maria Catarina M. Kasuya
Food Chemistry, 2012
Pleurotus ostreatus fungus forms an edible mushroom that possesses important nutritional and medi... more Pleurotus ostreatus fungus forms an edible mushroom that possesses important nutritional and medicinal properties. Selenium (Se) is essential to human diets and it is in low concentration in the soil, and consequently in food. P. ostreatus was grown in coffee husks enriched with various concentrations of sodium selenite. The biological efficiency of P. ostreatus was affected by the addition of high concentrations of Se. The highest level of Se absorption was obtained by adding 51 mg kg 1 of sodium selenite. The mushrooms from first flush contained more Se than the further flushes. These results demonstrate the great potential of coffee husks in the production of Se-enriched mushrooms and show the ability of this fungus to absorb and biomagnify Se.
Journal of Food Science, 2012
Mushrooms have effective mechanisms to absorb and accumulate trace elements from substrates and, ... more Mushrooms have effective mechanisms to absorb and accumulate trace elements from substrates and, therefore could be used as a strategy to produce mineral-enriched food and nutritional supplements. This study aimed to enrich shiitake mushrooms with selenium (Se), an important dietary element in human health. Strains of Lentinula edodes (Berk.) were grown on artificial logs composed of eucalyptus sawdust, and were subjected to cold shock in water containing sodium selenite (Na 2 SeO 3) at concentrations of up to 1.28 mM. The content of Se in the mushrooms increased linearly with increasing amounts of Na 2 SeO 3 added to the cold water although above 0.96 mM, mushroom formation was inhibited. Concentrations greater than 17 mg Se 100/g of dried mushrooms were observed after treatment with 0.64 mM Na 2 SeO 3. Shiitake mushroom had a demonstrate potential to offer an effective and economical way to produce Se-enriched products and, the strategy of adding selenite in cold water, used in this study, showed promising once it does not interfere with mycelial growth.
Anais da Academia Brasileira de Ciências, 2020
Effective microorganisms (EM) are inoculants formed by fungi and bacteria isolated from soil. EM ... more Effective microorganisms (EM) are inoculants formed by fungi and bacteria isolated from soil. EM are commonly used by farmers on agronomic crops to stimulate plant growth, but their composition and their benefi ts has been controverted. This study aimed to analyze the diversity of microorganisms growing in three EM inoculants, as well as to evaluate their effi ciency in the germination of palisade grass seeds. The total DNA of the three EM inoculants was extracted, the 16S rRNA and ITS genes were amplifi ed by PCR and sequenced on the Illumina MiSeq platform. Germination tests were conducted with three type of the EM, in three concentration and two times of the immersion. The bacterial group was the most abundant in EM, followed by fungi. Bacterial operational taxonomic units OTUs were shared by all EMs. Pre-treatments of palisade grass seeds with EMs resulted in a higher germination percentage (% G) and germination speed index (IVG) when EM was used at concentration of 1 or 2% in water. Seed immersion for 5 min was more effi cient than immersion for 24 h. We can conclude that EM of different origin can share microbial groups and diversity of microorganisms, besides being an alternative to increase palisade grass seeds germination.
BIOTROPIA, 1996
This work sought to verify the presence of compounds with antimicrobial properties in extracts of... more This work sought to verify the presence of compounds with antimicrobial properties in extracts of ectomycorrhizal fungi or in Picea glehnii inoculated with ectomycorrhizal fungi. Extracts from Pisolithus tinctorius, Scleroderma flavidum, Amanita pantherina and Paxillus sp., grown in liquid culture media, and from P. glehnii seedlings inoculated or not with the above ectomycorrhizal fungi and cultivated in in vitro condition, were processed to obtain two fractions, water and ethyl acetate solubles. These fractions were tested for the presence of inhibitory constituents against Fusarium roseum, Pythium sp. and Rhizoctonia solani. Direct bioautography technique on TLC or paper disc technique was used, depending on the extract and pathogenic fungi tested. The results showed the production on inhibitory components, not only by ectomycorrhizal fungi, but also by P. glehnii inoculated or not with ectomycorrhizal fungi. The sensitivity varied considerably according to the type of fungus or extract.
Revista Brasileira de Ciência do Solo, 2011
Rhizoctonia-like fungi are the main mycorrhizal fungi in orchid roots. Morphological characteriza... more Rhizoctonia-like fungi are the main mycorrhizal fungi in orchid roots. Morphological characterization and analysis of conserved sequences of genomic DNA are frequently employed in the identification and study of fungi diversity. However, phytopathogenic Rhizoctonia-like fungi have been reliably and accurately characterized and identified through the examination of the fatty acid composition. To evaluate the efficacy of fatty acid composition in characterizing and identifying Rhizoctonia-like mycorrhizal fungi in orchids, three Epulorhiza spp. mycorrhizal fungi from Epidendrum secundum, two unidentified fungi isolated from Epidendrum denticulatum, and a phytopathogenic fungus, Ceratorhiza sp. AGC, were grouped based on the profile of their fatty acids, which was assessed by the Euclidian and Mahalanobis distances and the UPGMA method. Dendrograms distinguished the phytopathogenical isolate of Ceratorhiza sp. AGC from the mycorrhizal fungi studied. The symbionts of E. secundum were gr...
African Journal of Microbiology Research, Mar 12, 2014
The detoxification of Jatropha curcas seed cake is of major interest for the biodiesel industry t... more The detoxification of Jatropha curcas seed cake is of major interest for the biodiesel industry to add economic value to this residue and also to reduce the environmental damage caused by its inappropriate disposal. In this context, the treatment of this residue with white rot fungus, Pleurotus ostreatus, can be a viable alternative because it produces enzymes capable of degrading different lignocellulosic residues and toxic compounds. In this study, the capacity of P. ostreatus to degrade phorbol esters found in Jatropha seed cake and the potential to transform this residue in animal feed was evaluated. After 60 days of incubation with the fungus, the phorbol ester concentration was reduced by 99% (final concentration of only 2 x 10-4 mg g-1 dry mass). This value is lower than the level observed in the non-toxic Mexican variety. Also, we showed that fungal growth improved some features desirable for animal feed, such as, increases the in vitro digestibility, decreases lignin and cellulose content and increases the protein content. Therefore, P. ostreatus is able to degrade phorbol esters found in Jatropha seed cake and has the potential to be used as animal feed.
African Journal of Microbiology Research, Sep 17, 2014
The cau oils (Gü Jatr for (Jo 200 *Co Aut 4.0I
Morphological and molecular characterization of Pisolithus occurring in Hokkaido Island, Northern Japan
Mycoscience, Oct 1, 2008
The ectomycorrhizae (EM) of Humaria and Genea, two closely related genera of the Pyronemataceae (... more The ectomycorrhizae (EM) of Humaria and Genea, two closely related genera of the Pyronemataceae (Ascomycetes), were regularly found in different deciduous forests of Hungary. In the present paper, the morphology and anatomy of these EM are described in detail, including morphometric analyses. Identification of the EM was carried out by molecular taxonomic analyses of the nrDNA ITS sequences obtained from mycorrhizae, herbarium ascomata, and public databases. The anatomy of the EM, examined during this work, was almost identical. They possessed angular outer and epidermoid inner mantle layers and warted, thick-walled emanating hyphae. Ten of our EM sequences grouped into the clade of Humaria hemisphaerica sequences and one into the genus Genea. Both molecular taxonomic analysis and morphometry differentiated three sub-groups within the clade of Humaria, and these methods also clearly separated the EM of Genea from those of Humaria. We may suppose that the previous morphological-anatomical descriptions, lacking molecular taxonomic identification, do not concern the denominated taxa. As a consequence, we stress the importance of revaluating the literature data, based on morphotyping of Humaria and Genea EM, to prevent misidentification in future studies. The presented work demonstrates that combining molecular and morphological analysis is essential for the unambiguous identification of the EM formed by problematic taxa.
Impacto do monocultivo de caf� sobre os indicadores biol�gicos do solo na zona da mata mineira
Cienc Rural, 2009
African Journal of Microbiology Research, Jul 9, 2014
In this work, we tested the ability of white-rot fungi to alter the chemical composition of ligno... more In this work, we tested the ability of white-rot fungi to alter the chemical composition of lignocellulosic agro-industrial residues so that they could be more susceptible to microbial degradation by rumen microorganisms, thus improving in vitro digestibility of neutral detergent fiber. Agro-industrial residues that were incubated for 60 days with Pleurotus ostreatus PLO06 or Lentinula edodes UFV73 showed significant changes in chemical composition, increasing (P<0.05) the crude protein content and the in vitro digestibility of neutral detergent fiber. Lignin content and structural carbohydrates decreased after fungal fructification in most substrates and an increase (P<0.05) in dry matter mineralization was also observed for residues treated with P. ostreatus PLO06. L. edodes UFV73 required a more balanced carbon/nitrogen ratio to grow on lignocellulosic substrates, and P. ostreatus PLO06 was in general, more effective than L. edodes UFV73 to alter the chemical composition and digestibility of the agroindustrial residues. Among the residues tested, eucalyptus bark showed the highest increase in digestibility after fungal growth. The marked increments in fiber digestibility obtained with substrates inoculated with either P. ostreatus PLO06 or L. edodes UFV73 indicate the usefulness of biological pretreatments to improve the nutritive value of low-quality lignocellulosic feedstuffs that could be incorporated into ruminant rations.
Psyche: A Journal of Entomology, 2012
The grass-cutting antAtta bisphaericais one of the most serious pests in several pastures and cro... more The grass-cutting antAtta bisphaericais one of the most serious pests in several pastures and crops in Brazil. Fungal diseases are a constant threat to these large societies composed of millions of closely related individuals. We investigated the occurrence of filamentous fungi associated with the antA. bisphaericain a pasture area of Viçosa, Minas Gerais State, Brazil. Several fungi species were isolated from forager ants, and two of them, known as entomopathogenic,Beauveria bassianaandAspergillus ochraceus, were tested against worker ants in the laboratory. The two species were highly virulent, achieving 50 percent worker mortality within 4-5 days. It is the first timeA. ochraceus, a commonly found fungal species, is reported to infectAttaspecies at a high prevalence. Possible uses for the fungus within biological control are discussed.
Journal of Food Research, 2012
Mushrooms have been used for food and medicinal purposes since ancient period. The β-glucans foun... more Mushrooms have been used for food and medicinal purposes since ancient period. The β-glucans found in mushrooms are currently considered to be responsible for some of the medicinal properties of mushrooms, thereby motivating studies on characterization of these compounds. In our study, we tested the effect of nitrogen supplementation on the productivity and nutritional composition of Pleurotus ostreatus mushrooms. The fungi were grown in various substrates supplemented with urea or rice bran, and the biological efficiency, mineral composition, protein and β-glucan content were evaluated. The growth of P. ostreatus in substrates with nitrogen supplementation increases the mushroom's productivity and nutritional value. Our results also indicate that nitrogen supplementation, a simple technique, efficiently increases the β-glucan content of mushrooms.
Revista Brasileira de Ciência do Solo, 2008
As espécies vegetais de Cerrado sensu stricto apresentam estratégias adaptativas às condições edá... more As espécies vegetais de Cerrado sensu stricto apresentam estratégias adaptativas às condições edáficas e climáticas de altos investimentos em fotoassimilados, nutrientes e água para sua estruturação. A simbiose entre fungos e raízes de plantas é uma importante adaptação radicular que auxilia as plantas na absorção de nutrientes e água do solo, sendo determinantes para a sobrevivência no Cerrado. Com o objetivo de estudar fungos micorrízicos arbusculares (FMAs) e fungos endofíticos do tipo dark septate (DSEFs) nas raízes de algumas espécies arbóreas e herbáceas, nativas do Cerrado sensu stricto, foram testados diferentes métodos para melhor observação das estruturas fúngicas em simbiose. O melhor método de clarificação foi observado quando as raízes foram autoclavadas a 121 °C em KOH 2 %, por 20 min, e com a subseqüente transferência para solução nova de KOH 2 %, por 24 h, em temperatura ambiente. Este procedimento foi repetido e, em seguida, essas amostras foram imersas em H2O2 2 % ...
Revista Brasileira de Ciência do Solo, 2012
Fungos micorrízicos arbusculares (FMAs) são fungos de solo, biotróficos obrigatórios e formadores... more Fungos micorrízicos arbusculares (FMAs) são fungos de solo, biotróficos obrigatórios e formadores da simbiose mutualista mais comum na natureza: a micorriza arbuscular (MA). Essa associação ocorre nas raízes da maioria das plantas terrestres, promovendo melhorias no crescimento, desenvolvimento e aumento na tolerância e, ou, resistência das plantas a vários agentes ambientais adversos. Além disso, os FMAs podem ser utilizados como potenciais agentes de controle biológico de doenças de plantas. Esses fungos produzem ainda glomalina, uma proteína que desempenha papel fundamental na estabilidade do solo e bioestabilização de solos contaminados. As diferentes respostas das plantas a essa simbiose podem ser atribuídas à diversidade funcional das MAs, em função da interação FMA-planta-condições ambientais. O estabelecimento e funcionamento da MA durante as condições de estresse envolvem um complexo processo de reconhecimento e desenvolvimento, concomitantemente às alterações bioquímicas, ...
Revista Brasileira de Ciência do Solo, 2005
Distúrbios causados pelo homem têm resultado no aumento do risco de extinção de diversos táxons d... more Distúrbios causados pelo homem têm resultado no aumento do risco de extinção de diversos táxons de orquídeas nativas da Mata Atlântica no Brasil. Na natureza, orquídeas utilizam obrigatoriamente fungos endomicorrízicos para a germinação de sementes e desenvolvimento da plântula, ao menos nos primeiros estádios do seu ciclo de vida. Assim, fungos micorrízicos associados ao sistema radicular de orquídeas nativas vêm sendo isolados, caracterizados e armazenados para uso em futuros programas de conservação de espécies de orquídeas, por meio da germinação simbiótica. Três isolados de fungos micorrízicos rizoctonióides foram obtidos do sistema radicular de três espécies de orquídeas neotropicais, Gomesa crispa, Campylocentrum organense e Bulbophyllum sp., de três diferentes fragmentos de Mata Atlântica no Brasil. Estudos taxonômicos, baseados na condição nuclear, morfologia da hifa vegetativa e ultra-estrutura do septo dolipórico, revelaram que os isolados pertencem aos gêneros Ceratorhiz...
Revista Brasileira de Ciência do Solo, 2005
Twenty-nine isolates of the ectomycorrhiza fungus Pisolithus sp. from different geographical and ... more Twenty-nine isolates of the ectomycorrhiza fungus Pisolithus sp. from different geographical and host origins were tested for their ability to form ectomycorrhizae on Eucalyptus grandis and E. urophylla seedlings under greenhouse conditions. The ectomycorrhiza-forming capacity of isolates varied greatly from one eucalypt species to the other. All isolates from Eucalyptus, nine from Pinus spp. and two isolates from unknown hosts formed mycorrhizae with E. grandis and E. urophylla. Root colonization rates varied from 0 to 5.2 % for all Pinus isolates and those from unknown hosts. Colonization rates for these isolates were lower than those observed for Eucalyptus isolates (0.8 to 89.4 %). Three isolates from unknown hosts formed mycorrhizae with neither Eucalyptus species. The main characteristic for distinguishing Pinus from Eucalyptus isolates was mantle color. These data corroborate previous results obtained in our laboratory indicating that the isolates tested represent at least tw...
Advances in Microbiology, 2013
The production of mushrooms enriched with essential elements, e.g. selenium, for human health is ... more The production of mushrooms enriched with essential elements, e.g. selenium, for human health is an interesting strategy to improve the functional foods supply. The selenium is an essential mineral and makes part of structure of enzymes involved in the oxidative metabolism. However, the selenium effect on the activity of respiratory and lignocellulolytic enzymes has not been considered. The understanding of this effect is important to determine the selenium concentration that increases the mushroom productivity and the degradation rate of the substrate. In this study, it was observed reduction of the respiratory activity of Lentinula edodes (Berk.) Pegler, the shiitake mushroom, in function of the increasing of the sodium selenite concentration in the substrate (p < 0.05). Selenium did not inhibit the activity of the hydrolytic enzymes (cellulase and xylanase), but it increased the activity of the oxidative enzyme (laccase). Respiratory activity of L. edodes has a negative correlation with sodium selenite concentration added in substrate. Thus is important to define the ideal dose of selenium to be added to the substrate for increasing lignocellulosic residues degradation and, consequently, guarantee a higher production of Se-enriched mushrooms.
PLoS ONE, 2014
Eucalypt cultivation is an important economic activity worldwide. In Portugal, Eucalyptus globulu... more Eucalypt cultivation is an important economic activity worldwide. In Portugal, Eucalyptus globulus plantations account for one-third of the total forested area. The nutritional requirements of this crop have been well studied, and nitrogen (N) is one of the most important elements required for vegetal growth. N dynamics in soils are influenced by microorganisms, such as diazotrophic bacteria (DB) that are responsible for biological nitrogen fixation (BNF), so the aim of this study was to evaluate and identity the main groups of DB in E. globulus plantations. Samples of soil and root systems were collected in winter and summer from three different Portuguese regions (Penafiel, Gavião and Odemira). We observed that DB communities were affected by season, N fertilization and moisture. Furthermore Bradyrhizobium and Burkholderia were the most prevalent genera in these three regions. This is the first study describing the dynamic of these bacteria in E. globulus plantations, and these data will likely contribute to a better understanding of the nutritional requirements of eucalypt cultivation and associated organic matter turnover.
Revista Ceres, 2010
A aplicação do glyphosate sobre a soja resistente a este herbicida pode causar prejuízos à simbio... more A aplicação do glyphosate sobre a soja resistente a este herbicida pode causar prejuízos à simbiose com o rizóbio. O objetivo deste trabalho foi avaliar a sensibilidade ao herbicida glyphosate de três estirpes de Bradyrhizobium recomendadas para a produção de inoculantes de sementes de soja no Brasil. Avaliou-se o efeito das concentrações de 0,0; 5,4; 10,8; 21,6 e 43,2 µg L-1 do ingrediente ativo do glyphosate [N-(fosfonometil) glicina] no meio YM líquido sobre o crescimento de B. japonicum (estirpe SEMIA 5079) e de B. elkanii (estirpe SEMIA 5019 e estirpe SEMIA 587), por meio de leituras das densidades óticas e geração de curvas de crescimento. As reduções de crescimento na presença da menor concentração do glyphosate foram de 18% para SEMIA 5079, 29% para SEMIA 5019 e de 35% para SEMIA 587, sendo, de modo geral, quanto maior a concentração do herbicida no meio de cultura maior a inibição do crescimento. As estirpes apresentaram sensibilidade diferencial somente às concentrações ma...
Revista Árvore, 2011
A associação micorrízica é advinda da associação simbiótica entre alguns fungos do solos e da mai... more A associação micorrízica é advinda da associação simbiótica entre alguns fungos do solos e da maioria das raízes das plantas. O eucalipto possui a capacidade de se associar com dois tipos de micorrizas, a micorriza arbuscular e a ectomicorriza, o que depende muito da sua idade. Este trabalho objetivou avaliar a taxa de colonização por fungos micorrízicos arbusculares (MA) e ectomicorrízicos (ECM) e o número de esporos de fungos micorrízicos arbusculares em plantios comerciais de Eucalyptus grandis e Eucalyptus urophylla, com diferentes idades e manejos, no período de dezembro de 2002 a fevereiro de 2004, na região leste de Minas Gerais, Brasil. Em todas as coletas e em todas as idades dos plantios, foram encontradas MA, e ECM e a média geral da colonização por fungos micorrízicos arbusculares (FMA) foi de 26%. O número médio de esporos desses fungos de 374,7 por 100 g de solo e a colonização por fungos ectomicorrízicos (FECM) de 20,2%. As maiores porcentagens de colonização por FECM...
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Papers by Maria Catarina M. Kasuya