Tomato plants grown in sterilized soil applied with Pseudomonas fluorescens grown on Jatropha cak... more Tomato plants grown in sterilized soil applied with Pseudomonas fluorescens grown on Jatropha cake resulted in highest recovery of P. flourescens from soil. Combined application of two bio-agents in the rhizosphere of tomato in sterilized soil resulted in comparatively lower recovery of P. fluorescens and Trichoderma harzianum but was found better for root growth, shoot growth and fruit yield enhancement than their single application. Combined application of bio-agents grown on any of the cake to the sterilized soil resulted in "three time" increase in root length, 1.5 times increase in shoot length and "five time" increase in fruit yields of tomato crop.
Deoiled cakes of Neem and Jatropha served as source of diversified nutrition for Pseudomonas flou... more Deoiled cakes of Neem and Jatropha served as source of diversified nutrition for Pseudomonas flourescens when used as substrate for mass culturing of antagonist. The present investigation were undertaken to test the suitability of neem and Jatropha cakes for liquid Concentration and longevity of Pseudomonas flourescens in vitro. Increasing the dilution of stock of initial inoculums resulted in decrease of total viable counts of Pseudomonas flourescens was comparatively highest population (64.67×10 5 ) and (201.67×10 5 ) lesser after 15 days than after 7 days of inoculation on Neem and Jatropha cake concentration respectively.
The present investigation was undertaken to isolate dif ferent strains of Pseudomonas fluorescens... more The present investigation was undertaken to isolate dif ferent strains of Pseudomonas fluorescens from various agroecological zones or crop’s rhizosphere like moong, brinjal, rice, chilli, mustard, chirchida and tomato. Totally eight micro flora resembling Pseudomonas fluorescenswere isolated and three isolates were confirmed as P. fluorescens (strain P.f.01, strain P.f.05 and strain P.f.07). Pseudomonas fluorescens strains P.f 07 were found most effective with the highest antagonistic activity against three fungal pathogen and show maximum inhibition of myceli al growth of Fusarium moniliforme (65.45%), Rhizoctonia solani (68.23%), and Alternaria alternate (48.13%).
Population dynamics of P. fluorescens on Mahua and Karanja cakes
Suitability of Mahua and Karanja cakes for mass multiplication and longevity of Pseudomonas flour... more Suitability of Mahua and Karanja cakes for mass multiplication and longevity of Pseudomonas flourescens in vitro was also tested. In this study, mahua cake was found to be best substrate for supporting the population dynamics and longevity of P. flourescens in vitro. Both these cakes, supported the population of P. flourescens up to 120 days. Highest population of P. flourescens was noticed on mahua cake after 45 days of inoculation when maintained with 35% moisture. On karanja cake, highest population of P. flourescens was noticed after 45 days of inoculation when maintained with 35% moisture. Increasing concentration of extract (20%) of both the cakes, resulted in increasing the population of P. flourescens. Tomato plants grown in sterilized soil applied with P. fluorescens grown on mixture of mahua and karanja cakes resulted in highest recovery of Pseudomonas flourescens from soil after 60 days of application
International Journal of Current Microbiology and Applied Sciences, 2017
Effect of different temperature and culture media was observed on all ten Trichoderma viride isol... more Effect of different temperature and culture media was observed on all ten Trichoderma viride isolates. The excellent growth of Trichoderma was found at temperature range of 25-30˚C. All the 10 Trichoderma isolates were evaluated on four different liquid media for assessing growth and sporulation. The maximum mycelial weight was found in potato dextrose broth (276.00 mg) and the lowest Czapek Dox broth (96.00 mg). Media, pH, Temperature, Trichoderma viride.
Journal of Data Mining in Genomics & Proteomics, 2014
Trichoderma species are widely used in agriculture as biopesticides. These fungi reproduce asexua... more Trichoderma species are widely used in agriculture as biopesticides. These fungi reproduce asexually by production of conidia and chlamydospores and in wild habitats by ascospores. Trichoderma species are well known for their production of enzymes called Cell Wall Degrading Enzymes (CWDEs). All living organisms are made up of genes that code for a protein which performs the particular function. Some genes that play an important role in the biocontrol process are known as the biocontrol genes. These genes send some signals which help in secretion of proteins and enzymes that degrade the plant pathogens. These biocontrol genes can be cloned in huge amounts and can be used on large scale for commercial production. Some Trichoderma genes are also helpful in providing resistance to the biotic and abiotic stresses such as heat, drought and salt .The major biocontrol processes include antibiosis, mycoparasitism and providing plant nutrition.
In vitro effect on chickpea seedling induced by biopriming of Trichoderma viride ie. the treatmen... more In vitro effect on chickpea seedling induced by biopriming of Trichoderma viride ie. the treatment T 1 (T. viride 5g/kg seed) was excelled overall significant superior performance by contributing 68%, 12.07cm, 16.37cm, 28.44cm, 0.592g, 1933.92 and 40.26 in germination, root length, shoot length, seedling length, dry weight, vigour index I and vigour index II, respectively. The highest protein content was observed in chickpea seedling that's bioprimed by Trichoderma viride formulation (T. viride @5g/kg seeds) was estimated through Kelplus analizer was 0.41%.The protein quantification of chickpea seedlings was estimated through Lowry and Bradford method were 1.06 and 1.08 mg /ml. The protein profiling of chickpea seedling on SDS PAGE were observed maximum five bands, first band at 250 kda, second band at 100 kda, third band was lying between 75 to50 kda, and fourth band was at 20 kda.
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