Luiz Rosa

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Antarctic cryosphere fungal diversity (2015)

Amplicon sequencing dataset (Illumina MiSeq) targeting Fungi (ITS) in ice samples (n=8) from the ... more

Revista Filosofia Capital-RFC ISSN

RESUMO Neste artigo analisamos de que maneira Nietzsche estabelece sua insólita investigação sobr... more RESUMO Neste artigo analisamos de que maneira Nietzsche estabelece sua insólita investigação sobre o tipo psicológico de Jesus através de um viés intuitivo, alheio aos parâmetros historiográficos e filológicos próprios da pesquisa teológica moderna, considerados falhos em decorrência das camadas hermenêuticas que os textos evangélicos receberam ao longo das eras. ABSTRACT In this article we analyze how Nietzsche establishes his unusual research about psychological type of Jesus through an intuitive bias, oblivious to the Philological and historical parameters of modern theological research, considered flawed due to the layers that Evangelical hermeneutics received throughout the ages.

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DNA Metabarcoding Reveals Cryptic Geographically-influenced Microbial Diversity After Anthropogenic Impact in Original Forest Soils on the Isolated Trindade Island, South Atlantic

Located 1,140 km from the South American coastline in the South Atlantic Ocean, and with an age o... more Located 1,140 km from the South American coastline in the South Atlantic Ocean, and with an age of 4 million years, Trindade Island is the most recent volcanic component of Brazilian territory. Its vegetation was severely damaged by human influence in particular through the introduction of exotic grazing animals such as goats. However, since the complete eradication of goats and other feral animals in the late 1990s, the island’s vegetation has been recovering and even some endemic species that had been considered extinct have been rediscovered. In this study we set out to characterize the contemporary microbial diversity of Trindade Island forest soils using metabarcoding by High Throughput Sequencing (HTS). Sequences of representative of two domains (Bacteria and Archaea) and five kingdoms (Fungi, Metazoa, Protozoa, Chromista and Viridiplantae) were identified. Bacteria were represented by 20 phyla and 116 taxa, while and Archaea by only one taxon. Fungi were represented by seven ...

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Fungi in the Antarctic Cryosphere: Using DNA Metabarcoding to Reveal Fungal Diversity in Glacial Ice from the Antarctic Peninsula Region

Microbial Ecology, 2021

We assessed fungal diversity present in glacial from the Antarctic Peninsula using DNA metabarcod... more We assessed fungal diversity present in glacial from the Antarctic Peninsula using DNA metabarcoding through highthroughput sequencing (HTS). We detected a total of 353,879 fungal DNA reads, representing 94 genera and 184 taxa, in glacial ice fragments obtained from seven sites in the northwest Antarctic Peninsula and South Shetland Islands. The phylum Ascomycota dominated the sequence diversity, followed by Basidiomycota and Mortierellomycota. Penicillium sp., Cladosporium sp., Penicillium atrovenetum, Epicoccum nigrum, Pseudogymnoascus sp. 1, Pseudogymnoascus sp. 2, Phaeosphaeriaceae sp. and Xylaria grammica were the most dominant taxa, respectively. However, the majority of the fungal diversity comprised taxa of rare and intermediate relative abundance, predominately known mesophilic fungi. High indices of diversity and richness were calculated, along with moderate index of dominance, which varied among the different sampling sites. Only 26 (14%) of the total fungal taxa detected were present at all sampling sites. The identified diversity was dominated by saprophytic taxa, followed by known plant and animal pathogens and a low number of symbiotic fungi. Our data suggest that Antarctic glacial ice may represent a hotspot of previously unreported fungal diversity; however, further studies are required to integrate HTS and culture approaches to confirm viability of the taxa detected.

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Tipos incondicionais de inferência e o conhecimento lógico

Veritas, 2016

In this paper I address the question 'How is knowledge of logical truths possible'. The s... more In this paper I address the question 'How is knowledge of logical truths possible'. The sought-after explanation should be (ideally) independent of what the true story about logical truth is. In particular, I try to account for the epistemic warrant that is conferred upon logical beliefs when they are neither inferred from other beliefs nor grounded on empirical evidence or testimony. The need for such an account is motivated by the apparent failure of the notions ofanalyticity on the one hand and intuition on the other in addressing the relevant question. I end up defending an account according to which warranted logical beliefs can be grounded on pure reasoning: they can be inferentially formed on the basis of pieces of suppositional reasoning.

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Schopenhauer e Nietzsche: Do Dualismo Metafísico Ao Princípio Da Unidade- Múltipla Schopenhauer and Nietzsche: The Metaphysical Dualism the Principle of Multiple-Unit

Making the first tragic existential philosophy, the doctrine of Schopenhauer ascribes the origin ... more Making the first tragic existential philosophy, the doctrine of Schopenhauer ascribes the origin of the simultaneously tragic, absurd and painful character of existence to wanting to live, implying a pessimism that imposes a negative condition to happiness, as the suffering emerges as the foundation of all life, becoming aesthetic pleasure as a possibility to overcome the pain and boredom, as noted in the article whose work shows the correlation involving the perspective of the metaphysics of will and Nietzsche's thought that, pausing in nihilism as a event that expresses the negation of life and converges to its own overcoming, overlaps the metaphysical dualism the principle of multiple-unit construction through its metaphysical artist, which implies a movement of ethical awareness for artistic pathos.

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Plataforma para Avaliação de Conexão de Geração Distribuída

Anais da XIV Conferência Brasileira sobre Qualidade da Energia Elétrica, 2021

Description of Dioszegia patagonica sp. nov., a novel carotenogenic yeast isolated from cold environments

International Journal of Systematic and Evolutionary Microbiology, 2017

During a survey of carotenogenic yeasts from cold and oligotrophic environments in Patagonia, sev... more During a survey of carotenogenic yeasts from cold and oligotrophic environments in Patagonia, several yeasts of the genus Dioszegia (Tremellales, Agaricomycotina) were detected, including three strains that could not be assigned to any known taxa. Analyses of internal transcribed spacer and D1/D2 regions of the large subunit rRNA gene showed these strains are conspecific with several other strains found in the Italian Alps and in Antarctica soil. Phylogenetic analyses showed that 19 of these strains represent a novel yeast species of the genus Dioszegia. The name Dioszegia patagonica sp. nov. is proposed to accommodate these strains and CRUB 1147 T (UFMG 195 T =CBMAI 1564 T =DBVPG 10618 T =CBS 14901 T ; MycoBank MB 819782) was designated as the type strain. This Dioszegia species accumulates biotechnologically valuable compounds such as carotenoid pigments and mycosporines.

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New Pesticidal Diterpenoids from Vellozia gigantea (Velloziaceae), an Endemic Neotropical Plant Living in the Endangered Brazilian Biome Rupestrian Grasslands

Molecules (Basel, Switzerland), Jan 21, 2017

Vellozia gigantea is a rare, ancient, and endemic neotropical plant present in the Brazilian Rupe... more Vellozia gigantea is a rare, ancient, and endemic neotropical plant present in the Brazilian Rupestrian grasslands. The dichloromethane extract of V. gigantea adventitious roots was phytotoxic against Lactuca sativa, Agrostis stolonifera, and Lemna paucicostata, and showed larvicidal activity against Aedes aegypti. Phytotoxicity bioassay-directed fractionation of the extract revealed one new isopimaradiene, 8(9),15-isopimaradien-1,3,7,11-tetraone, and three new cleistanthane diterpenoids, 7-oxo-8,11,13-cleistanthatrien-3-ol, 3,20-epoxy-7-oxo-8,11,13-cleistanthatrien-3-ol, and 20-nor-3,7-dioxo-1,8,11,13-cleistanthatetraen-10-ol. These new structures are proposed based on interpretation of ¹H, (13)C, COSY, NOESY, HSQC, and HMBC NMR data. 8(9),15-isopimaradien-1,3,7,11-tetraone was especially phytotoxic with an IC50 value (30 μM) comparable to those of commercial herbicides clomazone, EPTC, and naptalam. In addition, 7-oxo-8,11,13-cleistanthatrien-3-ol provided 100% mortality at a conc...

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Evolution of Mating Systems in Basidiomycetes and the Genetic Architecture Underlying Mating-Type Determination in the Yeast Leucosporidium scottii

Genetics, Jan 14, 2015

In most fungi, sexual reproduction is bipolar, that is, two alternate sets of genes at a single m... more In most fungi, sexual reproduction is bipolar, that is, two alternate sets of genes at a single mating-type (MAT) locus determine two mating types. However, in the Basidiomycota, a unique (tetrapolar) reproductive system emerged in which sexual identity is governed by two unlinked MAT loci, each of which controls independent mechanisms of self/nonself recognition. Tetrapolar to bipolar transitions have occurred on multiple occasions in the Basidiomycota, resulting for instance, from linkage of the two MAT loci into a single inheritable unit. Nevertheless, owing to the scarcity of molecular data regarding tetrapolar systems in the earliest-branching lineage of the Basidiomycota (subphylum Pucciniomycotina), it is presently unclear if the last common ancestor was tetrapolar or bipolar. Here, we address this question, by investigating the mating system of the Pucciniomycotina yeast Leucosporidium scottii. Using whole-genome sequencing and chromoblot analysis, we discovered that sexual ...

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Sex in the cold: taxonomic reorganization of psychrotolerant yeasts in the order Leucosporidiales

FEMS yeast research, 2015

Species of Leucosporidiales are a group of psychrotolerant yeasts with biotechnological potential... more Species of Leucosporidiales are a group of psychrotolerant yeasts with biotechnological potential. In the present work, we studied the phenotypic, genetic and sexual characteristics of three species of this genus (Leucosporidium scottii, Leucosporidiella creatinivora and Le. yakutica) to clarify the evolutionary relationship among these closely related taxa. From the results obtained, it becomes clear that these yeasts can interbreed. Although genetic delimitation is possible for the three species, the extent of nucleotide substitutions and phenotypic differences observed between them are lower than that expected for species that have ended the speciation process. Our taxonomic conclusion is to maintain the three taxa until further genomic data are gathered. However, the concept of L. scottii species complex is proposed for this group of species. Finally, we transfer all Leucosporidiella and Mastigobasidium species to Leucosporidium (Leucosporidiales), and, in order to end the polyp...

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Manipulação robótica na educação a distância

International Conference on Engineering and Computer Education, 2013

This paper presents the proposal, justification, specification and development of software to mon... more This paper presents the proposal, justification, specification and development of software to monitor and control a robot, designated to E-Learning, with an intuitive interface developed from the language of blocks thought the "LabView" platform. The proposed software is able to control the robot's movements and plans its routine. Throughout the alterations performed by the user at a remote terminal, the software sends the data via Internet to a computer connected to the robot, which receives the data and performs the scheduled task.

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Degradation of thiocyanate by immobilized cells of mixed and pure cultures

Biohydrometallurgy and the Environment Toward the Mining of the 21st Century, Pt B 1999, 1999

Thiocyanate is frequently found in industrial wastewater such as those from coal, gold and silver... more Thiocyanate is frequently found in industrial wastewater such as those from coal, gold and silver mining. Although the microbial degradation of thiocyanate has been well documented, scarce information is available on the heterotrophic degradation of thiocyanate. A pure bacteria strain (BMV8) and a mixed culture of bacteria and fungi were isolated by an enrichment method from a fixed bed bioreactor for cyanide and thiocyanate removal and they were able to metabolize thiocyanate heterotrophically as a nitrogen source. The efficiency of thiocyanate biodegradation decreased when the cells were immobilized in citric pectin. The immobilized mixed culture was able to degrade thiocyanate more efficiently than the BMV8 strain.

Biosorption of free and complexed cadmium ions by Aspergillus niger

Biohydrometallurgy and the Environment Toward the Mining of the 21st Century, Pt B 1999, 1999

ABSTRACT

Nematode‐induced galls in Miconia albicans : effect of host plant density and correlations with performance

Plant Species Biology, 2012

ABSTRACT The study of nematodes parasitizing native plants plays a crucial role in understanding ... more ABSTRACT The study of nematodes parasitizing native plants plays a crucial role in understanding plant–pathogen interactions. In the present study we describe the patterns of attack by an undescribed species of Ditylenchus occurring in Miconia albicans (Melastomataceae), a widespread, native shrub from the Brazilian cerrado. We also tested the hypothesis that nematode-induced leaf galls negatively correlate to host plant performance and that gall density is a function of host plant density. We collected paired healthy and attacked shoots from 28 individuals of M. albicans and estimated the leaf area lost to nematode-induced galls in up to 10 leaves per shoot. We analyzed the relationships between leaf area lost to nematode galls and reproductive traits. Nematode attack levels were also compared to the spatial distribution of the host plant. Inflorescence length and fruit production were significantly reduced in attacked shoots compared with healthy shoots. Seeds from attacked shoots showed no significant reduction in germinability or germination time when compared with seeds collected from healthy shoots. Gall density was positively correlated with host density. Despite being seldom studied in tropical ecosystems, nematodes may play an important role in plant fitness and in structuring tropical communities.

Gross Greenhouse Gas Emissions from Brazilian Hydro Reservoirs

Environmental Science

This paper presents the results of gross carbon dioxide and methane emission measurements in seve... more This paper presents the results of gross carbon dioxide and methane emission measurements in several Brazilian hydro reservoirs. The term 'gross emissions' means gas flux measurements from the reservoir surface without correcting for natural pre-impoundment emissions by natural bodies such as the river channel, seasonal flooding and terrestrial ecosystems. The net emissions result from estimating pre-existing emissions by the reservoir. Measurements were carried in the Miranda, Barra Bonita, Segredo, Três Marias, Xingó, Samuel and Tucuruí reservoirs, located in two different climatological regimes. Additional data were used here from measurements taken at the Itaipu and Serra da Mesa reservoirs. Emissions of carbon dioxide and methane in each of the reservoirs selected, whether through bubbles or diffusive exchange between water and atmosphere, were assessed by sampling, with subsequent extrapolation of results to obtain a value for the reservoir. A great variability was found in the emissions, linked to the influence of various factors, including temperature, depth at the point of measurement, wind regime, sunlight, physical and chemical parameters of water, the composition of the local vegetation and the operational regime of the reservoir.

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In vitro antifungal activities of leaf extracts of Lippia alba (Verbenaceae) against clinically important yeast species

Revista da Sociedade Brasileira de Medicina Tropical, 2014

Introduction: There are few studies reporting the antifungal activities of Lippia alba extracts. ... more Introduction: There are few studies reporting the antifungal activities of Lippia alba extracts. Methods: A broth microdilution assay was used to evaluate the antifungal effects of Lippia alba extracts against seven yeast species of Candida and Cryptococcus. The butanol fraction was investigated by gas chromatography-mass spectrometry. Results: The butanol fraction showed the highest activity against Candida glabrata. The fraction also acted synergistically with itraconazole and fl uconazole against C. glabrata. The dominant compounds in the butanol fraction were 2,2,5-trimethyl-3,4-hexanedione, 3,5-dimethyl-4-octanone and hexadecane. Conclusions: The butanol fraction may be a good candidate in the search for new drugs from natural products with antifungal activity.

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Antifungal and other Biological Activities from Oudemansiella Canarii(Basidiomycota)

World Journal of Microbiology and Biotechnology, 2005

The ethyl acetate extract from the fungus Oudemansiella canarii grown in malt extract medium was ... more The ethyl acetate extract from the fungus Oudemansiella canarii grown in malt extract medium was evaluated against (a) the recombinant enzyme trypanothione reductase from Trypanosoma cruzi, (b) lymphocyte proliferation in human peripheral blood mononuclear cells (PBMCs) stimulated with phytohaemaglutinin, (c) the human tumour cell lineages MCF-7, TK-10 and UACC-62, and (d) the phytopathogenic fungus Cladosporium sphaerospermum. At 10 lg/ml, the crude extract was inactive against PBMC but inhibited the growth of UACC-62 cells by 47% and the enzyme trypanothione reductase (TryR). It also presented strong inhibition in the bioautographic assay with C. sphaerospermum. Chromatographic fractionation guided by this assay allowed the isolation of oudemansin A (1), a known fungitoxic compound that showed a minimum inhibitory concentration (MIC) of 1.25 lg/spot in the bioautographic assay. As oudemansin A was not active in the other assays, other components in the extract may be responsible for the observed activities by the crude extract against the UACC-62 cells or the TryR enzyme.

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Coniochaeta ligniaria: antifungal activity of the cryptic endophytic fungus associated with autotrophic tissue cultures of the medicinal plant Smallanthus sonchifolius (Asteraceae)

Symbiosis, 2013

Fungal community associated with marine macroalgae from Antarctica

Polar Biology, 2009

Filamentous fungi and yeasts associated with the marine algae Adenocystis utricularis, Desmaresti... more Filamentous fungi and yeasts associated with the marine algae Adenocystis utricularis, Desmarestia anceps, and Palmaria decipiens from Antarctica were studied. A total of 75 fungal isolates, represented by 27 Wlamentous fungi and 48 yeasts, were isolated from the three algal species and identiWed by morphological, physiological, and sequence analyses of the internal transcribed spacer region and D1/D2 variable domains of the large-subunit rRNA gene. The Wlamentous fungi and yeasts obtained were iden-tiWed as belonging to the genera Geomyces, Antarctomyces, Oidiodendron, Penicillium, Phaeosphaeria, Aureobasid-ium, Cryptococcus, Leucosporidium, Metschnikowia, and Rhodotorula. The prevalent species were the Wlamentous fungus Geomyces pannorum and the yeast Metschnikowia australis. Two fungal species isolated in our study, Antarctomyces psychrotrophicus and M. australis, are endemic to Antarctica. This work is the Wrst study of fungi associated with Antarctic marine macroalgae, and contributes to the taxonomy and ecology of the marine fungi living in polar environments. These fungal species may have an important role in the ecosystem and in organic matter recycling.

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