Papers by Laurent Nussaume
Cracking the code of plant central phosphate signaling
Trends in Plant Science, Mar 1, 2023
Current Biology, Oct 1, 2017
Frontiers in Plant Science, Jan 20, 2017
SeedUSoon Software intellectual property, export data, and facilitate seed exchange between labor... more SeedUSoon Software intellectual property, export data, and facilitate seed exchange between laboratories. The SeedUSoon program, which is customizable to match individual practices and preferences, provides a powerful toolkit to plant laboratories searching for innovative approaches in laboratory management.

Research Square (Research Square), Jan 14, 2022
Coordinated distribution of Pi between roots and shoots is an important process that plants use t... more Coordinated distribution of Pi between roots and shoots is an important process that plants use to maintain Pi homeostasis. SHR (SHORT-ROOT) is well-characterized for its function in root radial patterning 1-3 . Here, we demonstrate a new role of SHR in controlling phosphate (Pi) allocation from roots to shoots by regulating PHOSPHATE1 (PHO1) in the root differentiation zone. We recovered a weak mutant allele of SHR in Arabidopsis which accumulates much less Pi in the shoot and shows constitutive Pi starvation response (PSR) under Pi-sufficient condition. Besides, Pi starvation suppresses SHR protein accumulation and releases its inhibition on the HD-ZIP Ⅲ transcription factor PHB. PHB accumulates and directly binds the promoter of PHO2 to upregulate its transcription, resulting in PHO1 degradation in the xylem-pole pericycle cells. Our findings reveal a previously unrecognized mechanism of how plants repress Pi translocation from roots to shoots in response to Pi starvation.
© 2003 Kluwer Academic Publishers. Printed in the Netherlands
AnewArabidopsis thaliana mutant deficient in the expression of O-methyltransferase impacts lignin... more AnewArabidopsis thaliana mutant deficient in the expression of O-methyltransferase impacts lignins and sinapoyl esters

Structure of chloroplast signal recognition particle and its role in chloroplast biogenesis
Signal recognition particle (SRP) is a cytoplasmic ribonucleoprotein that facilitates the co-tran... more Signal recognition particle (SRP) is a cytoplasmic ribonucleoprotein that facilitates the co-translational insertion of proteins into the endoplasmic reticulum in eukaryotes and the cytoplasmic membrane in prokaryotes. In prokaryotes, the SRP-RNA binds a single 54 kD polypeptide subunit, while in eukaryotes, five additional subunits are bound. A homologue of the 54 kD subunit of SRP was found in chloroplasts; it is 44% and 27% identical to SRP54 from E. coli and dog, respectively (1). This paper describes our progress in determining the structure of cpSRP and its role in the chloroplast. In particular we find that cpSRP, comprised of 43 and 54 kD subunits, plays a role in the biogenesis of light harvesting chlorophyll proteins (LHCP), the major proteins of the thylakoid membrane. LHCP form a large family of related proteins that have three to four transmembrane domains. They are synthesized in the cytoplasm, and are targeted to the thylakoid membrane through the stroma by a post-tra...

The EMBO Journal, 1996
Abscisic acid (ABA) is a plant hormone which plays an important role in seed development and dorm... more Abscisic acid (ABA) is a plant hormone which plays an important role in seed development and dormancy and in plant response to environmental stresses. An ABA-deficient mutant of Nicotiana plumbaginifolia, aba2, was isolated by transposon tagging using the maize Activator transposon. The aba2 mutant exhibits precocious seed germination and a severe wilty pheno- type. The mutant is impaired in the first step of the ABA biosynthesis pathway, the zeaxanthin epoxidation reaction. ABA2 cDNA is able to complement N.plumbaginifolia aba2 and Arabidopsis thaliana aba mutations indicating that these mutants are homologous. ABA2 cDNA encodes a chloroplast-imported protein of 72.5 kDa, sharing similarities with different monooxigenases and oxidases of bacterial origin and having an ADP-binding fold and an FAD-binding domain. ABA2 protein, produced in Escherichia coli, exhibits in vitro zeaxanthin epoxidase activity. This is the first report of the isolation of a gene of the ABA biosynthetic pathway. The molecular identification of ABA2 opens the possibility to study the regulation of ABA biosyn- thesis and its cellular location. Keywords: abscisic acid/Arabidopsis thaliana/carotenoid/ Nicotiana plumbaginifolialtransposable element
Proceedings of the National Academy of Sciences, Apr 30, 2002
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific r... more HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
From mycorrhization to Pi homeostasis control: PHR is the key player!
Science Bulletin, Mar 1, 2022
Proceedings of the National Academy of Sciences of the United States of America, Apr 3, 2003

Approaches and determinants to sustainably improve crop production
Food and Energy Security, Jan 26, 2022
Plant scientists and farmers are facing major challenges in providing food and nutritional securi... more Plant scientists and farmers are facing major challenges in providing food and nutritional security for a growing population, while preserving natural resources and biodiversity. Moreover, this should be done while adapting agriculture to climate change and by reducing its carbon footprint. To address these challenges, there is an urgent need to breed crops that are more resilient to suboptimal environments. Huge progress has recently been made in understanding the physiological, genetic and molecular bases of plant nutrition and environmental responses, paving the way towards a more sustainable agriculture. In this review, we present an overview of these progresses and strategies that could be developed to increase plant nutrient use efficiency and tolerance to abiotic stresses. As illustrated by many examples, they already led to promising achievements and crop improvements. Here, we focus on nitrogen and phosphate uptake and use efficiency and on adaptation to drought, salinity and heat stress. These examples first show the necessity of deepening our physiological and molecular understanding of plant environmental responses. In particular, more attention should be paid to investigate stress combinations and stress recovery and acclimation that have been largely neglected to date. It will be necessary to extend these approaches from model plants to crops, to unravel the relevant molecular targets of biotechnological or genetic strategies directly in these species. Similarly, sustained efforts should be done for further exploring the genetic resources available in these species, as well as in wild species adapted to unfavourable environments. Finally, technological developments will be required to breed crops that are more resilient and efficient. This especially relates to the development of multiscale phenotyping under field conditions and a wide range of environments, and use of modelling and big data management to handle the huge amount of information provided by the new molecular, genetic and phenotyping techniques.
Plant and Cell Physiology, Feb 8, 2019
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific r... more HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Author response: Stress induced gene expression drives transient DNA methylation changes at adjacent repetitive elements
Structure and regulation of nitrate reductase in Solanaceous species
HAL (Le Centre pour la Communication Scientifique Directe), 1994
International audienc
P4-3-5 Contribution of SKOR gene to Cs and K absorption and translocation in plants(4-3 植物の有害元素,2019年静岡大会)
日本土壌肥料学会講演要旨集, 2019
Figure 1-source data 1
Figure 1-source data 1: FPKM and fold change of all genes in rice roots and shoots upon Pi treatm... more Figure 1-source data 1: FPKM and fold change of all genes in rice roots and shoots upon Pi treatments
From mycorrhization to Pi homeostasis control: PHR is the key player!
Science Bulletin, 2021
Peer Review Report for 10.1105/tpc.19.00399 Arabidopsis ALIX Regulates Stomatal Aperture and Turnover of ABA Receptors
Review timeline: TPC2017-00689-RA Submission received: August 29, 2017 1st Decision: October 1, 2... more Review timeline: TPC2017-00689-RA Submission received: August 29, 2017 1st Decision: October 1, 2017 manuscript declined (might reconsider) TPC2019-00399-RA Submission received: May 21, 2019 1st Decision: June 25, 2019 accept with minor revisions TPC2019-00399-RAR1 1st Revision received: July 2, 2019 2nd Decision: July 12, 2019 acceptance pending, sent to sci editor Final acceptance: July 26, 2019 Advance publication: July 30, 2019

Mineral nutrient signaling controls photosynthesis: focus on iron deficiency-induced chlorosis
Trends in Plant Science, 2021
Photosynthetic organisms convert light energy into chemical energy stored in carbohydrates. To pe... more Photosynthetic organisms convert light energy into chemical energy stored in carbohydrates. To perform this process, an adequate supply of essential mineral elements, such as iron, is required in the chloroplast. Because iron plays a crucial role during electron transport and chlorophyll formation, iron deficiency alters photosynthesis and promotes chlorosis, or the yellowing of leaves. Intriguingly, iron deficiency-induced chlorosis can be reverted by the depletion of other micronutrients [i.e., manganese (Mn)] or macronutrients [i.e., sulfur (S) or phosphorus (P)], raising the question of how plants integrate nutrient status to control photosynthesis. Here, we review how improving our understanding of the complex relationship between nutrient homeostasis and photosynthesis has great potential for crop improvement.
“Je t'aime moi non plus": A love-hate relationship between iron and phosphate
Molecular Plant, 2021
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Papers by Laurent Nussaume