Papers by Hasan A A Khudhair
Medical Journal of Indonesia/Faculty of Medicine, Universitas Indonesia, 2025
BACKGROUND Recurrent pregnancy loss (RPL) is linked to lower vitamin D levels and altered immune ... more BACKGROUND Recurrent pregnancy loss (RPL) is linked to lower vitamin D levels and altered immune responses, though unclear mechanisms. This study aimed to identify the effect of vitamin D on the balance between interleukin (IL)-10/IL-17A in women with RPL. METHODS This case-control study was conducted at the Bint Al-Huda Teaching Hospital, Thi-Qar Governorate, Iraq, from August 2022 to March 2023. Three study groups were included: RPL (47 women), non-aborted pregnant (40 women), and control (38 women). The sera concentrations of IL-17A, vitamin D, and IL-10 had been quantitatively detected using ELISA. SPSS was used for statistical analysis. RESULTS Women with RPL had significantly lower vitamin D levels (19.6 ng/ml) and higher IL-17A levels (35.66 ng/l) than the non-aborted pregnant (23.46 ng/ml, 24.04 ng/l) and control groups (25.69 ng/ml, 19.87 ng/l). IL-10 levels were substantially depleted in the RPL group (3.96 pg/ml), leading to a statistically lower IL-10/IL-17A ratio (0.19) than in the non-aborted pregnant (0.58) and control (1.60) groups. Regression analysis revealed a valuable positive association between vitamin D and IL-17A in all groups and between vitamin D and IL-10/IL-17A ratio in RPL and control groups. Vitamin D was also significantly associated with IL-10 in the non-aborted pregnant group. However, no statistical relationship was reported between vitamin D and IL-10 levels in the RPL and control groups. CONCLUSIONS Increased IL-17A and decreased IL-10 contributed to unexplained RPL. The IL-10/IL-17A ratio predicted endometrial function, and vitamin D affects these cytokines, potentially reducing inflammation.
Journal of Applied Hematology/Wolters Kluwer - Medknow, 2024
BACKGROUND: The disease of sickle cell anemia (SCA) is an inherited blood disease that causes sev... more BACKGROUND: The disease of sickle cell anemia (SCA) is an inherited blood disease that causes several complications, and oxidative stress (OS) may play a very important role in its complications and pathophysiology. OBJECTIVE: The aims of the study is to evaluate the possible pathophysiological role of some antioxidant and oxidant biomarkers within SCA patients and to evaluate the correlation of OS and atherogenic index of plasma (AIP) to predict the cardiovascular risk in SCA subjects. MATERIALS AND METHODS: A case-control research includes three study groups: SCA patients, sickle cell trait (SCT) subjects, and healthy controls (HCs). All study groups were evaluated for their serum catalase (CAT), serum superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA) as well as plasma triglycerides and high-density lipoprotein cholesterol. Data were collected, tabulated, and statistically analyzed using (SPSS) version 31 program. The frequencies, relative frequencies, and means were obtained as descriptive methods. The associations among parameters were obtained using the Chi-square statistical method. RESULTS: The results revealed significantly lower levels of serum SOD and GPx among sickle cell disease (SCD) and SCT subjects than the HCs, whereas the inverse findings were reported for CAT and AIP. However, the MDA results revealed a significantly higher level in SCD patients compared to SCT and HC. Furthermore, the levels of SOD and MDA were positively associated in all study groups, and the levels of GPx and MDA were negatively correlated in SCD and SCT groups. The AIP was positively correlated with MDA in the SCT group and negatively correlated with CAT in the SCD group. CONCLUSIONS: The conclusion of the study indicated the presence of an OS and antioxidant compensatory status in patients with SCD and the SCT subjects. The altered biomarkers in SCA patients made it clear that the impairment and generation of OS in this disease, as well as antioxidant biomarkers, are contributory factors toward cellular redox homeostasis. This addressed the aim of the study.
University of Thi-Qar Journal Of Medicine, 2023
Type 1diabetes (T1D) occurs due to immune system dysregulation and invading pancreatic beta (β)-c... more Type 1diabetes (T1D) occurs due to immune system dysregulation and invading pancreatic beta (β)-cells by auto-reactive immune T cells, which leads to decreases of β-cells activity and viability in addition to prolonged therapy with exogenous insulin. The primary aim of this study was to delve into and explore the roles of adiponectin (ADP) and islet antigen-2 autoantibody (IA-2A) as potential predictors and/or diagnostic biomarkers for T1D and to determine the effects of them on pancreatic β-cells dysfunction. A case-control investigation was conducted between the months of August and December 2022, which included three study groups; the T1D group which included 35 newly diagnosed T1D patients, first degree relatives (FDRs) group which included a total of 35 FDRs of T1D patients and healthy control (HC) group with a total of 20 healthy individuals. Serum levels of connecting (C) peptide, ADP, and IA-2A were measured
EUROMEDITERRANEAN BIOMEDICAL JOURNAL, 2023
The research aimed to detect the roles of vitamin D (VD) and micro-ribonucleic acid (miRNA)-21-5p... more The research aimed to detect the roles of vitamin D (VD) and micro-ribonucleic acid (miRNA)-21-5p as potential predictors and diagnostic biomarkers for type 1 diabetes (T1D) and to determine the degree of association and predictability of them on beta (β) cells stress/death. A case-control study included three study groups: a T1D group that comprised 35 newly onset T1D patients, a firstdegree relatives (FDRs) group that included a total of 35 FDRs of T1D patients, and a healthy control (HC) group that included a total of 20 subjects. All study subjects were evaluated for their serum connecting (C) peptide and VD, as well as the expression folds of serum miRNA-21-5p. The findings revealed significantly lower levels of C. peptide and VD among T1D and FDRs subjects than the HC group, whereas the folds of miRNA-21-5p were significantly higher in T1D subjects than FDRs and HC subjects. Furthermore, the level of C. peptide had a valuable positive association with VD within T1D and FDRs groups, whereas the folds of miRNA-21-5p have a valuable positive association with C. peptide and VD in FDRs and T1D groups, respectively. Vitamin D had exhibited a significantly decreased level among T1D and FDRs, and positively correlated with residual β-cells function, which indicated the possible utility of its low level as a βcells stress/death predictive and diagnostic biomarker. Micro-RNA-21-5p indicated the possible utility of its high folds expression as β-cells stress/death predictive and diagnostic biomarker and the high level of miRNA-21-5p might play a vital role in T1D prevention.
University of Thi-Qar Journal of Science, 2023
The most frequent pregnancy issue is a miscarriage, which is defined as the spontaneous end of pr... more The most frequent pregnancy issue is a miscarriage, which is defined as the spontaneous end of pregnancy before the fetus reaches viability. The current research aims to identify the potential significance of selenium (Se) and vitamin E (VE) as predictive diagnostic biomarkers in recurrent pregnancy loss (RPL). Methods: A case control study that included three study groups; 35 women with RPL, 30 non-aborted pregnant (NAP) women, and 25 healthy women. After rigorously applying the eligibility requirements, patients were chosen from those visiting Bint Al-Huda Teaching Hospital and Suq Al-Shuyukh General Hospital in Thi-Qar Province (Iraq). Serum VE and serum Se levels were determined using the ELISA and atomic absorption spectrophotometer techniques, respectively. Results: The outcomes showed that the RPL group's serum VE and Se below normal levels were (88.6% and 62.9% respectively) lower than in the healthy control group (28% and 24% respectively). The level of VE was depleted in NAP group (93.3%) compared to HC group (28%) with significant differences, whereas the level of Se was significantly lower in RPL group (62.9%) compared NAP group (13.3%). Conclusions: A significant positive association of VE and Se in RPL and a significant negative association of VE and Se in NAP and HC.
University of Thi-Qar Journal of Science, 2023
Introduction: Helicobacter pylori (H. pylori) is a member of the causative factors of digestive s... more Introduction: Helicobacter pylori (H. pylori) is a member of the causative factors of digestive system complaints, such as gastritis, stomach ulcers, mucosal-associated lymphoid tissue lymphoma (MALT), and gastric cancer (GC). This research aimed to determine the H. pylori bacteria's possible roles in autoimmune gastritis (AIG) and assess the sera concentrations of vitamin B12 (VB12) and iron in individuals with and without H. pylori disease. Methods: In the Province of Thi-Qar, Iraq, case-control research was conducted during the time of November-2021 to June-2022, which included 55 subjects having H. pylori-associated diseases and 35 healthy individuals. The eligibility criteria of both study groups were followed stringently. For the patient's group, subjects underwent an endoscopic examination and a stool antigen test (SAT). Additionally, the subjects of all research cohorts were assessed for their sera levels of anti-H. Pylori-antibodies (Abs), parietal cells auto-Abs (PCA), VB12, and iron. Results: The findings revealed a significantly higher level of PCA positivity and lower levels of VB12 and iron by comparing the patient group with the control group. In both study groups, serum level of anti-H. pylori immunoglobulin gamma (IgG) Abs positively associated with PCA and inversely correlated with serum VB12 and iron in the patient's group. Conclusions: There were correlations between H. pylori infection and PCA-positive status, lower VB12, and low iron levels. Anti-H. pylori-IgG serum concentration was a valuable biomarker for the risk of developing AIG, low VB12, and iron levels. Parietal cells auto-Abs were not associated with VB12 and iron levels.
International Journal of Health Sciences, 2022
Background: Helicobacter pylori (H. pylori) is a multiflagellated, spiral-shaped, Gram-negative, ... more Background: Helicobacter pylori (H. pylori) is a multiflagellated, spiral-shaped, Gram-negative, microaerophilic, slowgrowing bacteria and extremely mobile which colonizes the stomach mucosa of a human where it causes a long-lasting infection which consistent with either acute or chronic stomach inflammation. Current study was aimed to evaluate total antioxidant capacity (TAC) and selenium (Se) levels in the serum of H. pylori infected individuals and to elucidate the relationship between the two biomarkers. Methods: Two study groups were enrolled in a current case control study; first group was included fifty five subjects with H. pylori infection which considered as H. pylori-associated patients (HPP), and the second group included thirty five apparently healthy subjects as healthy control (HC). Subjects were selected during the period between November 2021 and June 2022 after stringent application of the eligibility criteria. The HPP group subjects were underwent endoscopy and stool antigen test (SAT) and then both study groups subjects were evaluated for their serum anti-H. pylori antibodies (Abs), TAC and Se. Results: The findings revealed a significantly lower serum TAC and Se in HPP group in comparison with HC group. Serum immunoglobulin gamma (IgG) anti-H. pylori Abs was positively correlated with serum TAC in both study groups and negatively correlated with serum Se in HPP group. Serum TAC and Se levels were inversely correlated in both study groups. Conclusions: Oxidative stress (OS) was correlated with H. pylori infection with considerably reduced serum TAC and Se levels in HPP compared with HC, thereof these biomarkers may play particular roles in the pathophysiology, 4209 development, and flow up of the diseases which may be uses as supplements to improve the OS status of patients.
Journal of Medicinal and Chemical Sciences, 2022
Background: Gingivitis is an inflammation limited to marginal gingival tissues, which can be refe... more Background: Gingivitis is an inflammation limited to marginal gingival tissues, which can be referred to as the mildest form of periodontal diseases, initial signs of inflammation which include: redness, bleeding, swelling, exudation, and to a lesser extent pain, which indicate the presence of gingivitis. Current research was proposed to detect the correlation between pregnancy status and oxidative stress (OS) levels among gingivitis pregnant women and to elucidate the role of pregnancy status in the pathogenicity of gingivitis. Methods: The current study was conducted in the Dental Center at Thi-Qar province (Iraq), and it included three study groups: 35 pregnant women with gingivitis, 35 non-pregnant women with gingivitis, and 30 non-pregnant women without gingivitis (the healthy control), with an age balance between the three study groups. Serological and salivary levels of malondialdehyde (MDA), total anti-oxidant capacity (TAC), protein carbonyl (Pc), protein, nitric oxide (NO), and ceruloplasmin (Crp) were biochemically determined. Results: The results of the tests showed that the concentrations of MDA, Pc, the total proteins (TP), NO, and Crp were significantly higher among pregnant and non-pregnant women with gingivitis compared with healthy controls in both serum and saliva, whereas the inverse findings were reported for TCA biomarker. The same results profiles were reported in comparison between pregnant women with gingivitis and non-pregnant women with gingivitis for serum biomarkers. For saliva biomarkers, the results revealed significant elevated levels of MDA, Pc, and NO among pregnant gingivitis women in comparison with non-pregnant gingivitis, whereas the other biomarkers revealed non-significant differences. Conclusions: The pregnancy status had an effect on the OS level which lead to a higher level of OS in both serum and saliva and the correlation between the pregnancy status and serum and/or saliva OS levels was positive which indicated the possible utility of its present lead to gingivitis development.
Journal of Medicine and Life , 2023
Autoimmune rheumatoid arthritis (RA) is a systemic condition closely correlated with a variety of... more Autoimmune rheumatoid arthritis (RA) is a systemic condition closely correlated with a variety of autoantibodies (Abs) that could be considered diagnostic and prognostic markers. The current research was designed to detect the diagnostic values for a number (n) of these auto-Abs in RA detection and to evaluate the accuracy of a combined diagnostic scheme. This prospective study was conducted between September 2021 and August 2022 and included 110 subjects with RA, 70 individuals with other autoimmune disorders as positive controls (PC), and 50 unrelated, apparently healthy individuals as healthy controls (HC). The eligibility criteria for all study groups were followed stringently. An enzyme-linked immunosorbent assay (ELISA) was employed to measure rheumatoid factors (RF), cyclic citrullinated peptide antibodies (CCP-Abs), mutated citrullinated vimentin antibodies (MCV-Abs), anti-perinuclear factor antibodies (APF-Abs), and anti-keratin antibodies (AKA). We calculated the specificity, sensitivity, and predictive values of all auto-Abs. Significantly higher levels of anti-CCP-Abs, anti-MCV-Abs, APF-Abs, and AKAs were reported in the RA patients compared to the HC and PC subjects. RF levels, however, were only statistically elevated when compared to the HC individuals. Anti-APF-Abs had a higher sensitivity rate (70.9%), and anti-CCP-Abs had a higher specificity rate (94.16%) compared to other auto-Abs, whereas the combined detection scheme revealed a higher sensitivity (81.81%) and excellent specificity (90.83%) compared to the two former auto-Abs. Anti-perinuclear factor-Ab was a highly sensitive test, and CCP-Ab was a surpassingly specific assay for identifying RA. Furthermore, the combined detection scheme is an essential serological approach for RA diagnosis and crucial in differentiating this disease from other autoimmune diseases, thus promoting early diagnosis and treatment.
Journal of Education for Pure Science, 2017
This paper was aimed to investigate the association between the ABO blood groups and H. pylori in... more This paper was aimed to investigate the association between the ABO blood groups and H. pylori infection and to determine how much endoscopic findings are predict for the presence of active H. pylori infection. One hundred and twenty dyspeptic patients (as diagnosed by endoscopy and confirmed by histopathology) and sixty apparently healthy individuals were enrolled in this study. All subjects were underwent ABO blood grouping and Rhesus (Rh) systems determination. The results reveals higher frequency present of blood type O and the lowest frequency present of blood types A and AB among H. pylori patients compared to positive control group. The endoscopic findings showed low sensitivity in the diagnosis of H. pylori associated gastritis. In conclusion, there are considerable positive relationship between H. pylori infection and blood type O.
Proceedings of 2nd International Multi-Disciplinary Conference Theme: Integrated Sciences and Technologies, IMDC-IST 2021, 7-9 September 2021, Sakarya, Turkey, 2022
Klebsiella pneumoniae (K. pneumoniae) is a Gram-negative capsulated bacterium that causes a varie... more Klebsiella pneumoniae (K. pneumoniae) is a Gram-negative capsulated bacterium that causes a variety of human diseases. This study was to investigate the prevalence and antibiotic susceptibility pattern of K. pneumoniae isolated from diverse clinical samples, as well as to discover some of the bacteria's virulence components using a molecular approach. One hundred and eighty patients suffering from respiratory, urinary, wounds and burns infections were enrolled in this study and evaluated for the presence of K. pneumoniae infection via culturing, microscopical, motility and biochemical characteristics as well as API 20E and VITEK 2 systems. Molecular technology was used for detection the presence of K1 and K2 genes as well as the presence and sequencing 16S ribosomal ribonucleic acid (16S rRNA) gene. The results showed that a low prevalence of K. pneumoniae (27.8%) in Iraqi patients with higher positivity rate in sputum samples (42%) compared to other clinical samples.. According to the results of phylogenetic tree construction of 16S rRNA gene, we identified six mutated clinical isolates of K. pneumoniae that were deposited in GenBank under the accession numbers of MW642198, MW642199, MW642200, MW642201, MW642202 and MW642203. The mutated 16S rRNA gene stains exhibited a significantly higher resistance to all antibiotics types that used in this study.
Diabetes Mellitus type 1 (T1D) occurs due to disturbance intolerance of the immune system and inv... more Diabetes Mellitus type 1 (T1D) occurs due to disturbance intolerance of the immune system and invasion of β-cells by auto-reactive immune T cells and inducing deterioration of β-cells activity and viability and a prolong therapy with external insulin. This study was aimed to detect the predictive power as well as the degree of correlation of defined glutamic acid decarboxylase autoantibody (GADA), protein tyrosine phosphatase type A2 (IA-2A) and total antioxidant capacity (TAC) biomarkers on β-cells stress and/or death in T1D individuals as well as their first degree relatives (FDRs). Three groups of T1D patients, FDRs and apparently diseases free subjects had been enrolled in this work and evaluated for their serum GADA, IA-2A, C-peptide and TAC. The results revealed positive GADA and IA-2A in 88.6% and 40%, respectively, of T1D patients. The frequency of normal level and mean titer of C-peptide were significantly low among T1D and their FDRs, also the frequency of normal level and...
Annals of Tropical Medicine & Public Health, 2020
The enhanced body activities after taking the nutritional addendum by athletes were observed via ... more The enhanced body activities after taking the nutritional addendum by athletes were observed via fluctuation in the clinical biomarkers. There are some previous evidences recorded that the nutritional supplements have a benefit and side influences, hence current paper was aimed to determine the potential influences of nutritional support compounds on biochemical parameters and kidneys damage among bodybuilders people. Two subjects of sportsmen bodybuilders; first group not take the nutritional supplements (control group), whereas the second group take the nutritional supplements (users group) were included within current study and screened for their serological level of creatinine, urea, total protein and albumin with measuring urine total protein and pH. The results showed that the mean titer of serum creatinine, serum albumin and urine total protein were significantly higher among users group compared to control group, whereas the level of serum urea, serum total protein and urine pH revealed non-significant different between both groups. For subjects aware to the risks and side effects of taking the nutritional supplements, the users group exhibited a significant higher frequency % of unaware to the risks in comparison to control group. In denouement, there is prodromal defect in the kidneys of the nutritional supplements consumers, confirmed by serum creatinine, serum albumin and urine total protein increase. Many of nutritional supplements users not have enough information about the consequences and side effects of the decompounds hence exercising related with abuse of it without consulting a dietitian may be hazardous.
International Journal of Pharmaceutical Research, 2019
Diabetes Type 1 (T1D) is an autoimmune condition represent by pancreatic β-cells loss due to auto... more Diabetes Type 1 (T1D) is an autoimmune condition represent by pancreatic β-cells loss due to autoimmune processes, which reflected by presence of autoantibodies directed against islets β-cells auto-antigens. This study was aimed to identify the predictive power and the degree of correlation of genetic biomarkers (miRNA-25 and miRNA-375) on βcells stress and/or death in T1D individuals and their first degree relatives (FDRs). Three subjects of T1D individuals, FDRs and apparently healthy individuals were enrolled in this study and evaluated for their serum C-peptide and expression level of miRNA-25 and miRNA-375. The results showed that the frequency of normal level and mean titer of C-peptide were significantly low among T1D and their FDRs. The frequency of high level and mean titer of miRNA-25 were significantly low in both groups. For miRNA-375, the results revealed low level in 35% of T1D patient and in 10% of FDRs, whereas the mean titer was significantly higher among FDRs in comparison to HC. The miRNA-25 level was significantly lower in subjects with below normal C-peptide than subjects with normal or above normal C-peptide. Furthermore, miRNA-25 level was statistically highest in individuals with low miRNA-375 level than those with high level. Finally, a significant elevationinmiRNA-375 level was noticed among the individuals with below normal C-peptide compared to individuals with normal or above normal level of C-peptide. In conclusion, miRNA-25 had expressed a decreased level among the new onset T1D patients and their FDR, whereas miRNA-375 had expressed decreased level among newly onset T1D and elevated level among FDRs. The correlation between the residual β-cells mass and the miRNA-25 serum level was positive which indicate the possible utility of its low expression as a β-cells stress and/or death predictive biomarker. On the other hand the correlation between the residual β-cells mass and the miRNA-375 serum level was negative which indicate the possible utility of its high expression as a β-cells stress and/or death predictive biomarker.
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Papers by Hasan A A Khudhair