In the immediate vicinity of a slaughterhouse, a fishpond was built that uses treated wastewater ... more In the immediate vicinity of a slaughterhouse, a fishpond was built that uses treated wastewater from the slaughterhouse, and in this way integrates a new value chain in the form of purification and use of slaughterhouse wastewater in an aquaculture production system. The negative aspect of such integrated production systems is the concern related to the safety of fish meat produced in these systems. The aim of this research was to determine the activity level of Cs-137, K-40, Ra-226, Th-232, and Na-22 in water, sediment, and carp harvested from a pond that received purified water from a slaughterhouse. All samples were collected in spring and in autumn. The activity concentrations of selected radionuclides were determined by gamma spectrometry (HPG-e detector). The activity of K-40 in water samples ranged from 8.4 to 15.6 Bq L−1. The specific activity concentrations of the Ra-226 in water samples ranged between 1.02 ± 0.11 and 2.76 ± 0.49 Bq kg−1. The results of the activity of nat...
An experimental recirculating aquaculture system was constructed under ambient seawater condition... more An experimental recirculating aquaculture system was constructed under ambient seawater conditions to compare microbial community diversity of nitrifying and denitrifying biofilters that were derived from a commercial inoculum used for aquarium applications. Next generation sequencing revealed distinct and diverse microbial communities in samples analyzed from the commercial inoculant and the denitrification and nitrification biofilters. In all samples, communities were represented by a few dominant operational taxonomic units (OTUs). Bacteria having the capacity to carry out ammonia and nitrite oxidation were more abundant in the nitrification biofilter. Similarly, the proportion of the bacterial taxa known to carry out heterotrophic and autotrophic denitrification and participate in sulfur cycling were found in the denitrification bioreactor, and likely originated from the ambient environmental water source. Our
Recently, three females of the rare pelagic species Ocythoe tuberculata (Rafinesque, 1814) were r... more Recently, three females of the rare pelagic species Ocythoe tuberculata (Rafinesque, 1814) were recorded in the Eastern Adriatic Sea. One specimen was collected near the northern shore of the island Koločep in May 2006, while another was collected near the northern shore of the island Hvar in June 2006. The third individual was found near the northern shore of island Korčula in March 2007. Morphometric measurements, including beak dimensions, were recorded from all specimens after preservation. The smallest female (ML: 77 mm) was immature while the other two, with mantle lengths 178 and 202 mm, were mature females, for which potential fecundity estimates were 180 000 and 220 000 respectively. Oocyte size ranged from 0.1 to 3.9 mm, with oocytes smaller than 0.2 mm predominating in the ovary of both mature females. Remains of fish and gelatinous plankton organisms were found in the two non-empty stomachs examined. Occasional findings of O. tuberculata in the eastern Adriatic Sea are discussed in relation to the water masses circulation (ingressions from the eastern Mediterranean) and the species biological characteristics.
Population structure and growth parameters of Prussian carp (Carassius auratus gibelio) were stud... more Population structure and growth parameters of Prussian carp (Carassius auratus gibelio) were studied in 12 freshwater ecosystems of the Donbass region (Eastern Ukraine). These ecosystems differed significantly with respect to their surface area, water transparency and annual concentrations of phosphorus and nitrogen. Amongst the studied ecosystems, diploid males and females as well as a smaller percentage of triploid females were found. The population structure of C. a. gibelio varied significantly in terms of the percentage of triploids and sex ratio amongst diploids. A considerable proportion of triploid females ([10%) was found in four ecosystems with intermediate surface area (38-50 ha) and relatively high growth rate of specimens. The sex ratio amongst diploids was significantly femalebiased in seven of 12 ecosystems, including those where triploids were present in considerable numbers. The growth parameters of Prussian carp were significantly correlated with ecosystem characteristics, but the growth patterns of diploids and triploids were not significantly different from each other. The restricted distribution of triploid specimens suggests that the triploid form might counter diverse environmental challenges, whereas the diploid form of Prussian carp seems to be efficiently adapted to a wide range of ecosystem conditions.
Assessment of the impact of trout fish farm on the environment
Brojni cimbenici utjecu na stanje vodenog okolisa, ukljucujuci obogacenje hranjivim tvarima (eutr... more Brojni cimbenici utjecu na stanje vodenog okolisa, ukljucujuci obogacenje hranjivim tvarima (eutrofikacija), koje je prepoznato kao jedan od glavnih ekoloskih problema. Koristenje vodenog okolisa kao resursa za akvakulturu, predstavlja moguci put unosa hranjivih tvari u okolis. Mikroorganizmi su prvi izloženi antropogenim utjecajima kroz obogacenja hranjivim tvarima, sto rezultira mikrobnim oneciscenjem vodenog okolisa. Sediment ima sposobnost za prihvat cestica iz okolisa, organske tvari i mikroorganizama. Ispiranje mikroorganizama iz sedimenta i njihovo uzdizanje uzlaznom strujom u vodeni stupac otvorenih voda, promijeniti ce kvalitetu vode. Cilj ovih istraživanja bio je procijeniti utjecaj pastrvskog ribnjaka na okolis na osnovi pracenja bakterijskih pokazatelja oneciscenja u vodi i sedimentu, kao pokazateljima utjecaja pastrvskog ribnjaka na vodeni okolis. Osim bakterijskih pokazatelja (ukupnih koliforma, Escherichia coli, enterokoka i Pseudomonas aeruginosa, ukupnog broja bakterija) u vodi i sedimentu, određivani su fizikalno kemijski pokazatelji u vodi na pastrvskom ribnjaku i u pripadajucem rijecom okolisu, kao i granulometrijski i mineralni sastav, udio karbonata, te udio organske tvari u sedimentu. Rezultati ovog istraživanja ukazuju na promjenjivu mikrobiolosku kvalitetu rijecne vode koja ulazi u ribnjak (E. coli, ukupni broj bakterija). Na ribnjaku je utvrđeno obogacenje vode organskom tvari sukladno proizvodnom procesu, a s tim u vezi i povecanje broja ukupnih koliforma. Bazeni za taloženje imaju pozitivan ucinak na kvalitetu vode koja izlazi iz ribnjaka. Mineralni i granulometrijski sastav sedimenta u njima je u skladu sa sedimentom rijeke. Udio organske tvari u sedimentu uzgajalista je nesto visi od 4% (4, 05- 4, 18%), sto ukazuje na njen povecani unos u odnosu na rijeku gdje je udio oko 1-1, 5%. Broj ukupnih koliforma i ukupnog broja bakterija u sedimentu je veci nego u vodi koja izlazi iz ribnjaka. Rezultati ovog istraživanja, analiza sedimenta i rijecne vode nizvodno od ribnjaka ukazuju na lokalizirani utjecaj pastrvskog ribnjaka na samom uzgajalistu.
Microbiological assessment of Sea bass (Dicentrarchus labrax) farmed in the Adriatic Sea (Croatia)
Sea bass (Dicentrarchus labrax) is an important fish species farmed in many countries, including ... more Sea bass (Dicentrarchus labrax) is an important fish species farmed in many countries, including Croatia. Farming of healthy and quality Sea bass is the main aim in Sea bass aquaculture. This study documents the bacteria associated with Sea bass aquaculture during regular normal farming. The study was carried out on a commercial marine farm in the Malostonski bay of the Adriatic Sea. During the sampling period in 2016 and 2017, in total 80 specimens of the Sea bass were collected seasonally (spring, summer, autumn and winter). Samples for microbiological analysis were taken as swabs from the gills and skin using the sterile cotton sticks. All swab samples were serially diluted, plated and incubated on Marine agar (22°C), CHROMagar™ Acinetobacter (37°C), Slanetz-Bartley (35°C), Pseudomonas agar F (22°C) and Thiosulphate Citrate Bile Salt Sucrose (22°C) for isolation. Representative colonies were picked for further identification by MLADI TOF MS. The bacterial genera were predominantly Gram-negative and comprised mostly of Pseudomonas, followed by Acinetobacter, Vibrio and Aeromonas. The other, less numerous bacteria are from the genera Achromobacter, Arthrobacter, Microbacterium, Pantoea, Photobacterium, Providencia and Shewanella. Because the quality of fresh Sea bass is a major concern for the consumers, obtained results should allow a better control of the bacteriological parameters in the Sea bass aquaculture and also better control of processing to reduce the possible microbial risk.
The influence of farming technology on the meat quality and survival of the flat oyster, Ostrea edulis (Linneaus, 1758) in the Bay of Mali Ston
Abstract: The meat quality and the mortality rate of the European flat oyster, Ostrea edulis (Lin... more Abstract: The meat quality and the mortality rate of the European flat oyster, Ostrea edulis (Linnaeus, 1758) was investigated using two different farming technologies: the traditional method of cementing them on nylon ropes, and the alternative of placing the oysters at a ...
Rast i ponašanje grgeča, Perca flavescens (Michill, 1814) u ovisnosti o različitoj učestalosti hranjenja
ABSTRACT Juvenile yellow perch, Perca flavescens, was raised for four months in the closed recirc... more ABSTRACT Juvenile yellow perch, Perca flavescens, was raised for four months in the closed recirculation system under two different feeding regimes: continuous feeding, and feeding four times per day. Both groups were fed with the same daily amount of feed. Fish fed continuously grew faster and reached 99,35 ± 6,18 g in four months. The other group fed 4 times per day was significantly smaller at the end of the experiment reaching 82,54 ± 8,23 g. Conversion factor for the group fed continuously ranged from 0,8 at the beginning to 0,58 at the end of the experiment. For the fish fed four times per day conversion factor ranged from 0,68 to 0,47. The survival within the groups was not significantly different.
Growth and Behaviour of Juvenil Yellow Perch, Perca flavescens (Michill, 1814)Under Two Different Feeding Frequencies
Grgec, Perca flavescens, nasadne srednje mase od 25 g uzgajan je u zatvorenom recirkulacijskom su... more Grgec, Perca flavescens, nasadne srednje mase od 25 g uzgajan je u zatvorenom recirkulacijskom sustavu u dva hranidbena režima koja su uspoređena pracenjem rasta i preživljavanja. Ista dnevna kolicina hrane davana je u prvom režimu cetiri puta dnevno, a u drugom kontinuiranom kroz 16 sati. Riba hranjena kontinuirano statisticki je znacajno brže rasla i dosegla za tri mjeseca srednju masu od 99, 35 +- 6, 18g, za razliku od ribe hranjene cetiri puta dnevno koja je narasla na 82, 54 +- 8, 23g. Faktor konverzije hrane za ribe hranjene kontinuirano varirao je od 0, 8 na pocetku do 0, 58 na kraju pokusa. Za ribe hranjene istom kolicinom hrane cetiri puta dnevno izracunat je faktor konverzije od 0, 68 na pocetku i 0, 47 na kraju pokusa. Preživljavanje ribe u oba tretmana bilo je visoko (89, 5 % za ribz hranjenu cetiri puta dnevno i 91, 8 % za ribu hranjenu kontinuirano) i nije se statisticki znacajno razlikovalo ni unutar ni između ispitivanih skupina.
Non-indigenous species likely introduced by shipping into the Adriatic Sea
Marine Policy, Jul 1, 2021
Abstract By the intensification of maritime traffic, shipping could become one of the most likely... more Abstract By the intensification of maritime traffic, shipping could become one of the most likely pathways of introduction of non-indigenous species (NIS) in the Adriatic Sea. In this paper we provided an updated list of NIS introduced into Adriatic Sea by shipping as pathway, the most likely vectors of introduction, country of first detection, year, origin, establishing success and invasive characteristics. In total, 127 NIS were identified as the most probably introduced by maritime shipping into the Adriatic Sea. Rhodophyta, Arthropoda and Annelida represents almost 50% of all the listed species. The highest number of NIS introduced likely by shipping has been identified for period from 2001 to 2010 year. More than 73% of NIS listed for the first time in the Adriatic Sea were recorded in Italy. Half of listed NIS (50%) were probably introduced by external ship’s vector i.e. boat hull fouling (BHF). Majority of these species were classified as established. Screening of the potential risk of NIS being invasive in the Mediterranean and Adriatic Sea has not been done systematically, and for the most of NIS has never been performed. Increased number of sailing, motor recreational boats and cruise ships, lack on solutions for biofouling suppression and lack on regulations for anchoring may intensify NIS introductions by shipping as the most probable pathway into Adriatic Sea. That could become serious threat for native biodiversity and natural heritage, and it needs to be taken seriously by all relevant organisations and stakeholders.
Vibrio spp. have an important role in biogeochemical cycles; some species are disease agents for ... more Vibrio spp. have an important role in biogeochemical cycles; some species are disease agents for aquatic animals and/or humans. Predicting population dynamics of Vibrio spp. in natural environments is crucial to predicting how the future conditions will affect the dynamics of these bacteria. The majority of existing Vibrio spp. population growth models were developed in controlled environments, and their applicability to natural environments is unknown. We collected all available functional models from the literature, and distilled them into 28 variants using unified nomenclature. Next, we assessed their ability to predict Vibrio spp. abundance using two new and five already published longitudinal datasets on Vibrio abundance in four different habitat types. Results demonstrate that, while the models were able to predict Vibrio spp. abundance to an extent, the predictions were not reliable. Models often underperformed, especially in environments under significant anthropogenic influence such as aquaculture and urban coastal habitats. We discuss implications and limitations of our analysis, and suggest research priorities; in particular, we advocate for measuring and modeling organic matter.
Aquaculture provides more than 50% of all seafood for human consumption. This important industria... more Aquaculture provides more than 50% of all seafood for human consumption. This important industrial sector is already under pressure from climate-change-induced shifts in water column temperature, nutrient loads, precipitation patterns, microbial community composition, and ocean acidification, all affecting fish welfare. Disease-related risks are also shifting with important implications for risk from vibriosis, a disease that can lead to massive economic losses. Adaptation to these pressures pose numerous challenges for aquaculture producers, policy makers, and researchers. The dataset AqADAPT aims to help the development of management and adaptation tools by providing (i) measurements of physicochemical (temperature, salinity, total dissolved solids, pH, dissolved oxygen, conductivity, transparency, total nitrogen, ammonia, nitrate, nitrite, total phosphorus, total particulate matter, particulate organic matter, and particulate inorganic matter) and microbiological (heterotrophic (...
The rapid development and intensive production of aquaculture have brought to light various envir... more The rapid development and intensive production of aquaculture have brought to light various environmental problems. In particular, fish farming adversely affects the benthic environments through the accumulation of organic matter (OM), which leads to the degradation of aquatic sediments and associated biotic communities. To prevent environmental degradation, proper site selection for fish farming is a prerequisite for sustainable aquaculture practices. An important physical factor in selecting a site for fish farming is sediment structure. Coarser sediments are preferred, while their mineral composition is rarely discussed. To investigate relationships between sediment characteristics and the content of OM, sediments were collected from three different sites with different aquaculture practices under different climatic and environmental conditions and analyzed during the one-year monitoring period. Grain size distribution, mineral composition, carbonate content, and organic matter c...
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Papers by Ana Gavrilovic