Papers by Françoise Watteau
Introduction du Workshop Imagerie des Sols
The amending potential of vermicompost, compost and digestate from urban biowaste: Evaluation using biochemical, Rock-Eval® thermal analyses and transmission electronic microscopy
Bioresource Technology Reports
Carbon transfer from root and shoot litter to microorganisms in earthworm casts –TEM and NanoSIMS analyses
HAL (Le Centre pour la Communication Scientifique Directe), 2017
ImageSoil: a new database to boost explorations of the links between soil structure and soil functioning
HAL (Le Centre pour la Communication Scientifique Directe), Jul 31, 2022
Spatial and temporal distribution of 13C labelled plant residues in soil aggregates and Lumbricus terrestris surface casts: A combination of Transmission Electron Microscopy and Nanoscale Secondary Ion Mass Spectrometry
HAL (Le Centre pour la Communication Scientifique Directe), Jul 2, 2012
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific r... more HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Multi-scale approach of the structure evolution of constructed technosols during early pedogenesis
HAL (Le Centre pour la Communication Scientifique Directe), Jul 2, 2012

Localization and characterization by electron energy loss spectroscopy (EELS) of the brown cellular substances of beech roots
Soil Biology & Biochemistry, Oct 1, 1996
Degradation of roots from beech trees was investigated by a novel approach: localization and elem... more Degradation of roots from beech trees was investigated by a novel approach: localization and elemental characterization of predominant brown substances were performed at ultrastructural scale using electron energy loss spectroscopy (EELS). This technique allowed us to detect light elements such as C and N, main constituents concerned in the degradation of plant tissues, within cellular constituents observed with a transmission electronic microscope, after having localized them by light microscopy. The results showed that there was no relationship between the brown pigmentation of the cellular substances and the presence of N, a relationship previously found in studies of degrading leaves. Other elements such as Si and Ca were also found to be involved in these root degradation processes. This approach seem powerful for detailed descriptions of the process of root degradation in soil.
Journal of Environmental Quality, Jul 1, 2017
Studying soil development in ion-adsorbed REE mine tailings as tool to assess the sustainability of revegetation
HAL (Le Centre pour la Communication Scientifique Directe), May 22, 2017
Effects of two earthworms from two ecological groups on organic matter dynamics of a constructed Technosol
IV-4 Pedological engineering as a tool for management of urban ecosystems(IV Technogenic soils developed on settlements, soil formation, properties and utilization)
日本土壌肥料学会講演要旨集, Sep 9, 2014

Geoderma, Mar 1, 2019
Artificial soils constructed from wastes and by-products have been considered as a sustainable op... more Artificial soils constructed from wastes and by-products have been considered as a sustainable option for land reclamation. In particular, they could contribute in a complementary manner to natural soils to global climate regulation by storing large quantities of carbon (C). However, the evolution of C stocks in such newly formed soils remains largely unknown. This work aimed at evaluating the dynamics of C in constructed Technosols, focusing on two experimental sites in Lorraine, France, where Technosols were constructed from thermallytreated industrial soil, papermill sludge and green waste compost, and planted with grasses. Soil samples were collected over 12 years, and stocks of C were calculated, taking into account the increase in soil bulk density and the associated decrease in soil thickness over the years. The evolution of the stocks of organic C was compared to the evolution reported for natural grassland soils from the same region and for other Technosols. Initial organic C stocks in the two constructed Technosols were 50% higher than in natural analog soils when calculated over 30 cm, and up to 5 times higher when calculated over 100 cm. Organic C stocks in the two Technosols decreased over the first three years, but increased during the following years, most likely due to the accumulation of plantderived C. A similar evolution was observed in other planted Technosols. We conclude that Technosols constructed from an adequate set of parent materials can keep large amounts of organic C over time and sequester additional C under permanent plant cover.
Technosol micropedology : how micrometric interfaces reveal pedogenetic processes in man-made soils
HAL (Le Centre pour la Communication Scientifique Directe), Nov 27, 2016
Characterization of Soil Biodiversity hosted in Abandoned and Revegetated REEs Mine Sites In Southern China
HAL (Le Centre pour la Communication Scientifique Directe), Jun 16, 2019
Soils in urban environments: characteristics, services and problems of their investigation
Soils in urban environments: characteristics, services and problems of their investigation. SSP C... more Soils in urban environments: characteristics, services and problems of their investigation. SSP Congres Annuel

Soil Biology & Biochemistry, Feb 1, 2016
Earthworms transform organo-mineral associations in soil, especially by incorporating fresh resid... more Earthworms transform organo-mineral associations in soil, especially by incorporating fresh residues inside casts where the microbial abundance and activity are enhanced. The heterogeneous distribution of organic carbon in these structures influences decomposition levels at the microscale. The incorporation of 13 C labelled plant residues by Lumbricus terrestris inside cast was investigated, through the innovative combination of two fine scale imaging techniques: transmission electron microscopy and nanoscale secondary ion mass spectrometry (NanoSIMS). The association of these methods sheds new lights on organo-mineral structures. Different types of organic matter (plant residues, microbial remains) were identified in the casts and the freshly incorporated residues could be differentiated from the indigenous organic matter thanks to d 13 C NanoSIMS mapping. 13 C labelled bacteria and fungi abundance and diversity highlight their preeminent role in litter decomposition within casts. Labelled plant residues observed at various stages of decomposition and microorganisms presented highly variable d 13 C values, emphasizing the complexity of organic matter dynamics and the importance of microscale analyses to describe this variability. Thus, the combination of NanoSIMS and TEM shows great potential to relate organic matter stages of decomposition with their 13 C signature.
Spanish Journal of Soil Science, Jul 13, 2018
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific r... more HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

Applied Soil Ecology, Dec 1, 2018
Forest ecosystems can spontaneously establish themselves on former industrial sites contaminated ... more Forest ecosystems can spontaneously establish themselves on former industrial sites contaminated by metals. The sustainable management of these sites by natural attenuation requires a better understanding of the relationships between soil biodiversity, metals and soil functioning in such ecosystems. In this context, a forest ecosystem growing on iron industry by-products containing potentially toxic metals as major elements was studied using a multiproxy approach including chemical soil properties, microstructure, meso-and macro-fauna. Fauna communities in the topsoil were characterized by a high abundance and taxonomic diversity, but also by an unusual predominance of epigeic and detritivorous species. Fauna community structures were partly explained by the presence not only of heavy metals (Zn, Cd), but also Fe and Mn, dominant metals in iron industry by-products. The uncommon structure of macrofauna communities could have effects on the soil functioning, such as limiting the incorporation and mixing of organic matter into the soil. Mesofauna communities contributed to the metal redistribution in the soil by integrating metals into organo-mineral associations. In terms of management, this study showed the feasibility of using natural attenuation to manage sites with high metal contents but low metal availability and favorable conditions for plant growth. However, the influence of soil biota on metal mobility and soil functioning needs to be further investigated in order to assess the sustainability of such management.

Frontiers in Environmental Science, Apr 24, 2019
Earthworms play a major role in litter decomposition, in processing soil organic matter and drivi... more Earthworms play a major role in litter decomposition, in processing soil organic matter and driving soil structure formation. Earthworm casts represent hot spots for carbon turnover and formation of biogeochemical interfaces in soils. Due to the complex microscale architecture of casts, understanding the mechanisms of cast formation and development at a process relevant scale, i.e., within microaggregates and at the interface between plant residues, microorganisms and mineral particles, remains challenging. We used stable isotope enrichment to trace the fate of shoot and root litter in intact earthworm cast samples. Surface casts produced by epi-anecic earthworms (Lumbricus terrestris) were collected after 8 and 54 weeks of soil incubation in mesocosms, in the presence of 13 C-labeled Ryegrass shoot or root litter deposited onto the soil surface. To study the alteration in the chemical composition from initial litter to particulate organic matter (POM) and mineral-associated organic matter (MOM) in cast samples, we used solid-state 13 C Nuclear Magnetic Resonance spectroscopy ( 13 C-CPMAS-NMR) and isotopic ratio mass spectrometry (EA-IRMS). We used spectromicroscopic approach to identify plant tissues and microorganisms involved in plant decomposition within casts. A combination of transmission electron microscopy (TEM) and nano-scale secondary ion mass spectrometry (NanoSIMS) was used to obtain the distribution of organic carbon and δ 13 C within intact cast sample structures. We clearly demonstrate a different fate of shoot-and root-derived organic carbon in earthworm casts, with a higher abundance of less degraded root residues recovered as particulate organic matter on the short-term (8 weeks) (73 mg•g -1 in Cast-Root vs. 44 mg•g -1 in Cast-Shoot). At the early stages of litter decomposition, the chemical composition of the initial litter was the main factor controlling the composition and distribution of soil organic matter within casts. At later stages, we can demonstrate a clear reduction of structural and chemical differences in root and shoot-derived organic products. After 1 year, MOM clearly dominated the casts (more than 85% of the total OC in the MOM fraction). We were able to highlight the shift from a system dominated by free plant residues to a system dominated by MOM during cast formation and development.
Uploads
Papers by Françoise Watteau