Papers by Dr L. Srikanth SRMC
ADaPT: Adaptive Development and Prototyping Technique
The transmission line towers are one of the important life line structures in the distribution of... more The transmission line towers are one of the important life line structures in the distribution of power from the source to the various places for several purposes. The predominant external loads which act on these towers are wind and earthquake loads. In this present study tower is analyzed using Indian Standards IS: 875:1987(Wind Load), IS: 802:1995(Structural steel), IS:1893:2002 (Earthquake) and dynamic analysis of tower has been performed considering ground motion of 2001 Bhuj Earthquake (India). The dynamic analysis was performed considering a tower system consisting two towers spaced 800m apart and 35m height each. This analysis has been performed using numerical time stepping finite difference method which is central difference method were employed by a developed MATLAB program to get the normalized ground motion parameters includes acceleration, frequency, velocity which are important in designing the tower. The tower is analyzed using response spectrum analysis.
In view of the biological prominence of benzotriazole derivatives, it is planned to synthesize ne... more In view of the biological prominence of benzotriazole derivatives, it is planned to synthesize new benzotriazole derivatives. So, some new 2-(1H-benzo[d][1,2,3]triazol-1-yl)-N'-(2-substituted benzylidene) acetohydrazides (Va-Vg) have been synthesized as depicted in scheme-I. The intermediates and final compounds were purified and their chemical structures have been confirmed by IR, 1 H NMR, Mass and by elemental analysis. All the newly synthesized compounds were screened for their CNS activity (Gross behavioral studies and loco motor activity). Among the compounds tested, compound Vb with 4-Chloro substitution on the phenyl ring showed more promising depressant activity among all the test compounds followed by Vg and Ve.
In Staphylococcus
aureus the regulation of pyruvate kinase activity by serine/threonine protein kinase favors biofi... more aureus the regulation of pyruvate kinase activity by serine/threonine protein kinase favors biofilm formation
Synthesis And Evaluation Of Newer Quinoline Derivatives Of Thiazolidinediones For Their Antidiabetic Activity
International journal of pharma and bio sciences, 2010
In view of various biological activities of both thiazolidinediones and quinoline derivatives lik... more In view of various biological activities of both thiazolidinediones and quinoline derivatives like antidiabetic, antifungal, antitumoral, antiinflamm atory, retinoidal, anti atherosclerotic activities, it was our interest to prepare various thiazolidinedione derivatives with a quinoline ring moiety and to evaluate them for antidiabetic activity. 5�(4�fluor obenzyl)�2,4�thiazolidinedione was synthesized by reaction of 4�fluoroaniline with methyl acrylate to give crude 2�Bromo�3�(4�fluorophenyl)�propionic acid methyl ester as an oil which was treated with thiourea in presence of sodium acetate and ethanol to give 5�(4�fluorobenzyl)�2�iminothiazolidine�4� one which on oxidation gave 5�(4�fluorobenzyl)� thiazolidine�2,4�dione. This was condensed with the quinoline derivatives in presence of Tetrahydrofuran. Among the synthesized derivatives five of them were screened for oral hypoglycemic activity, the compounds SK, SK�3 were showing significant activity and compound SK� 2 was showing m...
Journal of Clinical & Scientific Research, 2013
Background: In Staphylococcus aureus purine metabolism plays a crucial role in the formation of b... more Background: In Staphylococcus aureus purine metabolism plays a crucial role in the formation of biofilm which is a key pathogenic factor. The present study is aimed in the characterization of inosine monophosphate dehydrogenase (IMPDH) from Staphylococcus aureus ATCC 12600. Methods: IMPDH gene was amplified using primers designed from IMPDH gene sequence of S. aureus reported in the database. Then polymerase chain reaction (PCR) product was cloned in the Sma I site of M13mp18 and expressed in Escherichia coli JM109. The recombinant IMPDH (rIMPDH) was overexpressed with 1 mM isopropyl beta-D-1thiogalactopyranoside (IPTG); Michaelis constant (Km), maximum enzyme velocity (Vmax) and catalytic constant (Kcat) of expressed IMPDH were determined. Results: The enzyme kinetics of IMPDH grown under aerobic conditions showed a Km of 43.71±1.56 µM, Vmax of 0.247±0.84/µM/mg/min and Kcat of 2.74±0.015/min while in anaerobic conditions the kinetics showed Km of 42.81±3.154/ µM, Vmax of 0.378±0.036 µM/mg/min and Kcat of 4.78±0.021/min, indicating elevated levels of IMPDH activity under anaerobic conditions. Three-folds increased activity in the presence of 1 mM adenosine triphosphate (ATP) correlated with biofilm formation. The kinetics of pure rIMPDH were close to the native IMPDH of S. aureus ATCC12600 and the enzyme showed single band in sodium dodecyl sulphate polyacrylamide gel electrophoresis with a molecular weight of 53 KDa. Conclusions: Elevated activity of IMPDH was observed in S. aureus grown under anaerobic conditions and this was correlated with the biofilm formation indicating the linkage between purine metabolism and pathogenesis.
Journal of Reinforced Plastics and Composites, 2007
Degradation in mechanical properties (compression, flexural and ILS) of glass epoxy composite lam... more Degradation in mechanical properties (compression, flexural and ILS) of glass epoxy composite laminates with induced defects (simulating delaminations) was studied. The defects were characterized by using the A-scan ultrasonic pulse-echo technique. The two A-scan parameters, viz. the back wall echo amplitude and time of flight, were followed respectively, as functions of the defect size (diameter) and its location in the laminate thickness. The mechanical properties of laminates were evaluated destructively for different defect sizes and locations. An attempt has been made to express the mechanical properties in terms of the two NDT scan parameters characterizing the defects and empirical equations presented.
Journal of Composite Materials, 2013
Investigations were carried out on glass-epoxy composite specimens with varied fibre orientations... more Investigations were carried out on glass-epoxy composite specimens with varied fibre orientations (±2° to ±88°), prepared by both braided and filament winding techniques. The experimental strength and stiffness of these composites while exhibiting similar trends, correlated well with those predicted by classical laminate theory. The filament wound composites exhibited (20–40%) initial higher properties vis-a-vis the braided composites, while a correlation index between the experimental and theoretical values has been evolved.
3 Biotech, 2014
Staphylococcus aureus, a natural inhabitant of nasopharyngeal tract, survives mainly as biofilms.... more Staphylococcus aureus, a natural inhabitant of nasopharyngeal tract, survives mainly as biofilms. Previously we have observed that S. aureus ATCC 12600 grown under anaerobic conditions exhibited high rate of biofilm formation and L-lactate dehydrogenase activity. Thus, the concentration of pyruvate plays a critical role in S. aureus, which is primarily catalyzed by pyruvate kinase (PK). Analyses of the PK gene sequence (JN645815) revealed presence of PknB site in PK gene indicating that phosphorylation may be influencing the functioning of PK. To establish this hypothesis the pure enzymes of S. aureus ATCC 12600 were obtained by expressing these genes in PK 1 and PV 1 (JN695616) clones and passing the cytosolic fractions through nickel metal chelate column. The molecular weights of pure recombinant PK and PknB are 63 and 73 kDa, respectively. The enzyme kinetics of pure PK showed K M of 0.69 ± 0.02 lM, while the K M of PknB for stpks (stpks = NLCNIPCSALLSSDITASVNCAK) substrate was 0.720 ± 0.08 mM and 0.380 ± 0.07 mM for autophosphorylation. The phosphorylated PK exhibited 40 % reduced activity (PK = 0.2 ± 0.015 lM NADH/ min/ml to P-PK = 0.12 ± 0.01 lM NADH/min/ml). Elevated synthesis of pyruvate kinase was observed in S. aureus ATCC 12600 grown in anaerobic conditions suggesting that the formed pyruvate is more utilized in the synthesis phase, supporting increased rate of biofilm formation.
International Journal of Current Pharmaceutical Research, 2017
Objective: A reversed phase liquid chromatography was determined and validated for the estimation... more Objective: A reversed phase liquid chromatography was determined and validated for the estimation of Mirabegron in tablet dosage form.Methods: The validation study of RP-HPLC showed a simple, rapid, accurate, precise, reproducible results by using a stationary phase: Waters Acquity HSS T-3 C18 (100 × 2.1 mm, 1.7μm and Mobile Phase-Potassium di-hydrogen phosphate: acetone in the ratio (40:60 v/v) at PH6.0±0.02. Detection is carried out at 243 nm using UV detector.Results: The total chromatographic analysis time per sample was about 6 min with Mirabegron eluting at a retention time of 2.754. Tailing factor obtained from the standard injection is 1.6. Theoretical Plates obtained from the standard injection is 2736.7. The flow rate is 1 ml/min and linearity in the concentration range of 30-70μg/ml (R2=0.999). The precision was 0.4% the intermediate precision was 0.08%. The deliberately varied chromatographic conditions in the concentration range for the evaluation of robustness is 1...
Transplant International, 2019
Context and Aim: Low therapeutical adherence (TA) to immunosuppressive drugs is one of the main f... more Context and Aim: Low therapeutical adherence (TA) to immunosuppressive drugs is one of the main factors that determine the survival of heart transplant recipients (HTx). The aim of this study is to measure the TA improvement by means of a comprehensive care programme in a multidisciplinary environment, together with the support of Mobile Health Technology (mHealth). Materials and methods: A prospective pilot study was carried out. Patients who had received a HTx in the past 18 months and had a mobile device were included. TA was assessed by the SMAQ validated test at the beginning and end of the two-months follow-up. Personalized interventions on-line via the mHeart platform were performed. The mHeart platform is an APP and a website directed to facilitate communication, treatment adherence and patient empowerment. An independent statistician analysed the data (IBM-SPSS V22.0). Results and discussion: Of the 35 eligible recipients, 32 (91.4%) were included in the study; 23 (71.9%) were men of an average age of 52.4 years [42.9-63.7] taking a median of 12 [8.5-14] different daily drugs. The follow-up time was 2.03 months [1.3-2.5]. The effectiveness of the pharmaceutical interventions implemented through the mHeart tool was high: 83% of the nonadherent recipients in the first visit became adherent at the end of the study according to the SMAQ test. Medication adherence global rate in the first visit was 61%, increasing to 87% at the last visit according to the SMAQ test (p = 0.039). The final TA figure was over 85%, the target medication adherence figure in solid organ transplants to avoid negative results due to non-adherence. Conclusions: Personalized interventions together with the support of the mHeart tool, permits effective supervision of nonadherent patients, resulting in the improvement in TA. The final TA figure was over 85%, the target figure in SOT to avoid negative results due to non-adherence.
IP Annals of Prosthodontics and Restorative Dentistry, 2021
said that, "You have to be able to fabricate things, you have to be able to analyze things, and y... more said that, "You have to be able to fabricate things, you have to be able to analyze things, and you have to be able to handle things smaller than ever imagined in ways not done before". Science is presently undergoing a great evolution, taking humanity to a new era: The era of nanotechnology. Nanotechnology is the field of science and technology pertaining to the creation and use of materials or devices at nanometer scale. Nanoscale is small in size, but its potential is vast. The growing interest in the future of dental application of nanotechnology leads to emergence of "Nanodentistry" which involves the maintenance of oral health by the use of nanomaterials, biotechnology and dental nanorobotics. Nanotechnologies are increasingly used for surface modifications of dental implants. This paper is an attempt to give an overview about the nanomaterials and nanotechnology and its applications in the field of Prosthodontics.
IP Annals of Prosthodontics and Restorative Dentistry, 2021
Background: osseointegration of dental implants refers to direct structural and functional link b... more Background: osseointegration of dental implants refers to direct structural and functional link between living bone and the surface of non-natural implants. It follows bonding up of an implant into jaw bone when bone cells fasten themselves directly onto the titanium surface.it is the most investigated area in implantology in recent times. Evidence based data revels that osseointegrated implants are predictable and highly successful. This process is relatively complex and is influenced by various factors in formation of bone neighbouring implant surface. Keywords: Osseointegration, Implant, Bone.
International Journal of Oral Health Dentistry, 2021
The power, predictability and speed that today's digital solutions offer to human oral health... more The power, predictability and speed that today's digital solutions offer to human oral health care have gained a rapidly growing foothold in clinical dentistry. One such revolutionary innovation is the rapid prototyping technique. This technique facilitates the fabrication of physical models from computer aided design (CAD) data using 3D printers. This technique enables the dentist and laboratory technician to produce dental-related prosthesis with a greater speed and accuracy, thus making it a reliable alternative to the much labour intensive and time consuming hands on procedure. The present article reviews this current technology, its historical development, methods and applications in dentistry.
Design and development of LabVIEW based environmental test chamber controller
2017 International Conference on Electrical, Electronics, Communication, Computer, and Optimization Techniques (ICEECCOT), 2017
Polymer composite materials are the most sought after materials for aerospace applications in vie... more Polymer composite materials are the most sought after materials for aerospace applications in view of their superior specific stiffness and specific strength properties. However, these materials are susceptible to the combined effects of temperature and humidity. Monitoring and control of these two parameters in an environmental test chamber, within a close tolerance for a stipulated duration to simulate the service conditions on the material is an essential part of qualification procedure for composites. At the Environmental Test Facility of CSIR-NAL, hitherto only data acquisition was possible through the computer, but the control activity required the operator to move physically to access the controller and provide the necessary change in inputs. The limitations have been successfully addressed by the design and implementation of a LabVIEW based software for monitoring as well as control of temperature and humidity in the environmental test chamber. This approach has eliminated the use of the existing DAQ and retransmission module hardware and further the controller itself is used as a DAQ device facilitating the control and functionalities such as data acquisition, storage, retrieval and presentation through LabVIEW software. Further, the need for physical movement of the operator to access the controller has been eliminated. In this paper, the development of a closed loop control algorithm and data acquisition protocol using LabVIEW is presented. The LabView has been used with an iTool Modbus Open Platform Communication (OPC) server for monitoring and control of temperature & humidity in the environmental test chamber. The innovative and novel controller has made the environmental conditioning process more efficient and cost effective. The accuracy achieved using the newly developed ETC is ± 0.5°C for temperature and ± 1% RH in case of humidity vis-avis the accuracy of ± 2°C and ± 3% RH, achieved with the old controller.
Mutations and structural variations in catechol-O-methyltransferase gene of patients exhibiting chronic persistent surgical pain
Revista Española de Anestesiología y Reanimación (English Edition), 2021
Abstract Objectives Mutations in the exon 4 of the COMT gene are associated with chronic persiste... more Abstract Objectives Mutations in the exon 4 of the COMT gene are associated with chronic persistent surgical pain (CPSP). Especially COMT mutated allele G472A (Val158Met) associated with CPSP patients is reported in different ethnic population. The purpose of this study is to evaluate the prevalence of genetic mutations and structural variations in exon 4 of COMT that can be related to the appearance of CPSP in patients under sternotomy. Materials and methods One hundred patients with American Society of Anesthesiologists (ASA) physical status grades I, II and III, who underwent sternotomy procedures, were selected to assess the development and magnitude of the CPSP evaluated with pain questionaries’ at the end of three months after surgery. This was correlated with COMT allele presence. The exon 4 of COMT gene (that contains the G472A allele) was studied. The polymerase chain reaction (PCR) products were sequenced and mutated sequences were deposited in GenBank®. The structural analysis of COMT was performed using ProCheck® and distortions of three-dimensional tertiary structural orientation was evaluated with root-mean-square deviation (RMSD) score. Results Genetic analysis carried out through PCR showed 220 bp amplicons. The 25% of patients with CPSP showed a Numeric Rating Scale (NRS) >4 pain score. 20% of these patients have known Val158Met mutation, 5% of patients showed novel mutations c.382C>G, c.383G>C; p. (Arg128Ala). The mutations in COMT gene contributed major structural variations in COMT leading to the formation of inactive COMT that correlates with CPSP. Conclusion The results of the present study showed that both novel and previously reported mutations in COMT gene has strong association with CPSP.
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Papers by Dr L. Srikanth SRMC