Papers by Chandrasekhar Kuppam
Multiproduct Algal Biorefineries: Challenges and Opportunities
Clean Energy Production Technologies, 2020

Applications of Nanomaterials and Future Prospects for Nanobionics
Nanotechnology is an interesting field in which molecules that are not visible to our eyes are cr... more Nanotechnology is an interesting field in which molecules that are not visible to our eyes are creating wonders. They exist in various fields of engineering and science such as electronics, agriculture, food, pharmaceuticals, and medical diagnostics, and many researchers are eyeing provision of better technology with these nanotechnological inventions. Nanomaterials show great promise, owing to their unique physicochemical properties, in the field of agriculture. Plants interact with these nanoparticles, resulting in various morphological, physiological, and genotoxic changes. Understanding of their interactions is very important for real gains in the potential use of nanotechnology in agriculture. Nanoparticles have been described as “magic bullets.” Nanoparticles can play a vital role in the growth of plants and help in the process of photosynthesis. They also influence the key processes that take place in the growth of plants, such as seed germination, root initiation, and the ph...
Use of chalcogenides-based nanomaterials for wastewater treatment including bacterial disinfection and organic contaminants degradation
Chalcogenide-Based Nanomaterials as Photocatalysts, 2021

International journal of molecular sciences, Jan 14, 2015
The current fossil fuel-based generation of energy has led to large-scale industrial development.... more The current fossil fuel-based generation of energy has led to large-scale industrial development. However, the reliance on fossil fuels leads to the significant depletion of natural resources of buried combustible geologic deposits and to negative effects on the global climate with emissions of greenhouse gases. Accordingly, enormous efforts are directed to transition from fossil fuels to nonpolluting and renewable energy sources. One potential alternative is biohydrogen (H2), a clean energy carrier with high-energy yields; upon the combustion of H2, H2O is the only major by-product. In recent decades, the attractive and renewable characteristics of H2 led us to develop a variety of biological routes for the production of H2. Based on the mode of H2 generation, the biological routes for H2 production are categorized into four groups: photobiological fermentation, anaerobic fermentation, enzymatic and microbial electrolysis, and a combination of these processes. Thus, this review pri...

Plant-based meat analogue (PBMA) as a sustainable food: a concise review
European Food Research and Technology, 2021
The global community is in a quest for nutritional and environment-friendly resources as a part o... more The global community is in a quest for nutritional and environment-friendly resources as a part of their food habit. The ubiquitous trend of veganism tied with the increasing apprehensions towards animal welfare, negative impact on human health and the environment has escalated the demand for meat alternatives mainly plant-based meat analogues (PBMA). Protein-rich bioresources such as cereals, vegetables, and algae have been explored to mimic animal meat in a similar flavour, texture, sensory and aromatic properties. This review aims to summarize the recent advancements in functional food technology based on vegetal proteins, a comparative account of traditional and commercially available meat alternates. The literature search for the last 10 years shows the rise in research on plant ingredients to develop novel human foods. A brief account of various production methods and their processing effects to improve the structural and techno-functionality of PBMA is suggested for designing sustainable food. The different combinations of plant and animal proteins are discussed to enhance the nutritional aspect, organoleptic profile and shelf-life of available food products. The positive feedback resulted in booming food industries across the world, incorporating vegetal proteins. The global market trend introducing well-established and promising food brands is listed to discuss the prospects of PBMA.
Algal Bioeconomy: A Platform for Clean Energy and Fuel
Industrial Saline Wastewater Treatment Technologies Using Microalgae Biomass
Removal of Pollutants from Saline Water
Algae—The Potential Future Fuel: Challenges and Prospects
Microbial Applications Vol.1
Biohydrogen Production: Integrated Approaches to Improve the Process Efficiency
Microbial Applications Vol.1

Phytotherapic Drugs for COVID-19 Treatment: A Scoping Review
Current Pharmaceutical Design
Background: The rapid eruption of Coronavirus at the end of 2019 has caused global health crisis ... more Background: The rapid eruption of Coronavirus at the end of 2019 has caused global health crisis and significant loss to the economy and social well-being. This created a massive shortage of advanced health facilities with inadequate medicinal supply, further deteriorating human health conditions. On the basis of adverse effects of the ongoing pandemic, this review has been proposed to evaluate the antiviral efficacy of plant- based therapeutics, against SARS-CoV-2 (commonly called COVID-19) infection. It highlights the possible action of the mechanism of phytotherapeutic drugs against coronavirus inhibition, further validated by clinical trials on herbal formulas is reviewed. Though the experimental studies on COVID-19 treatment are limited, the undesirable side effects of herbal drugs and unidentified compounds cannot be ignored. Objective: We have made an effort to study the prospective plant-derived bioactive entities and their effectiveness in the treatment of COVID-19 and also...
Greener synthesis of enzymes from marine microbes using nanomaterials
Handbook of Greener Synthesis of Nanomaterials and Compounds
Simultaneous production of astaxanthin and lipids from Chlorella sorokiniana in the presence of reactive oxygen species: a biorefinery approach
Biomass Conversion and Biorefinery
Simultaneous production of flavonoids and lipids from Chlorella vulgaris and Chlorella pyrenoidosa
Biomass Conversion and Biorefinery
A comprehensive review on two-stage integrative schemes for the valorization of dark fermentative effluents
Critical Reviews in Biotechnology

Production of biofuels from microalgae - A review on cultivation, harvesting, lipid extraction, and numerous applications of microalgae
Renewable and Sustainable Energy Reviews
The concern regarding alternate sources of energy is mounting day-by-day due to the effect of pol... more The concern regarding alternate sources of energy is mounting day-by-day due to the effect of pollution that is damaging the environment. Algae are a diverse group of aquatic organisms have an efficiency and ability in mitigating carbon dioxide emissions and produce oil with a high productivity which has a lot of potential applications in producing biofuel, otherwise known as the third-generation biofuel. These third generation biofuels are the best alternative to the present situation since they have the perspective to eliminate most of the ecological problems created by the use of conventional fossil fuels. These organisms are responsible for closely 50% of the photosynthesis process taking place on the planet and are distributed predominantly in many of the aquatic systems. The huge interest in utilizing these organisms as a potential source of energy lies in converting the primary as well as secondary metabolites into useful products. Algae are considered to be the most prominent resource for the upcoming generations as the most suitable and sustainable feedstock. The key process limitations in microalgal biofuel production are inexpensive and effective harvesting of biomass and extraction of lipids. The major objective of this article is to provide a comprehensive review on various methods of both biomass harvesting and lipid extraction from microalgae available, so far, besides to discuss their advantages and disadvantages. This article also deals with various conditions that are favourable for lipid accumulation as well as the yield from different species.

Solid phase bio-electrofermentation of food waste to harvest value-added products associated with waste remediation
Waste Management, 2015
A novel solid state bio-electrofermentation system (SBES), which can function on the self-driven ... more A novel solid state bio-electrofermentation system (SBES), which can function on the self-driven bioelectrogenic activity was designed and fabricated in the laboratory. SBES was operated with food waste as substrate and evaluated for simultaneous production of electrofuels viz., bioelectricity, biohydrogen (H2) and bioethanol. The system illustrated maximum open circuit voltage and power density of 443 mV and 162.4 mW/m(2), respectively on 9 th day of operation while higher H2 production rate (21.9 ml/h) was observed on 19th day of operation. SBES system also documented 4.85% w/v bioethanol production on 20th day of operation. The analysis of end products confirmed that H2 production could be generally attributed to a mixed acetate/butyrate-type of fermentation. Nevertheless, the presence of additional metabolites in SBES, including formate, lactate, propionate and ethanol, also suggested that other metabolic pathways were active during the process, lowering the conversion of substrate into H2. SBES also documented 72% substrate (COD) removal efficiency along with value added product generation. Continuous evolution of volatile fatty acids as intermediary metabolites resulted in pH drop and depicted its negative influence on SBES performance. Bio-electrocatalytic analysis was carried out to evaluate the redox catalytic capabilities of the biocatalyst. Experimental data illustrated that solid-state fermentation can be effectively integrated in SBES for the production of value added products with the possibility of simultaneous solid waste remediation.

Biohydrogen, 2013
The technically feasibility of converting organic pollutants in wastewater into hydrogen by a con... more The technically feasibility of converting organic pollutants in wastewater into hydrogen by a continuous two-step process was demonstrated. Two carbohydrates, i.e. glucose and sucrose, in wastewater were respectively acidified by dark fermentation at pH 5.5 with 6-6.6 hours of hydraulic retention in a 3-l fermentor, producing an effluent containing mostly acetate and butyrate, and a methane-free biogas comprising mostly hydrogen. The acidified effluent was then further treated by photo fermentation for hydrogen production. The overall yield based on the substrate consumed was 31-32%, i.e. 17-18% for dark fermentation and 14% for photo fermentation. It was found that under certain dark fermentation conditions, hydrogen-producing sludge was agglutinated into granules, resulting in a higher biomass density and increased volumetric hydrogen production efficiency. DNA-based analysis of microbial communities revealed that the respective predominant bacteria were Clostridium in dark fermentation and Rhodobacter in photo fermentation. Further investigations are warranted, particularly, in areas such as improving reactor design, treating protein and lipid rich wastewaters, and studying sludge granulation mechanisms and controlling factors.

The current fossil fuel-based generation of energy has led to large-scale industrial development.... more The current fossil fuel-based generation of energy has led to large-scale industrial development. However, the reliance on fossil fuels leads to the significant depletion of natural resources of buried combustible geologic deposits and to negative effects on the global climate with emissions of greenhouse gases. Accordingly, enormous efforts are directed to transition from fossil fuels to nonpolluting and renewable energy sources. One potential alternative is biohydrogen (H2), a clean energy carrier with high-energy yields; upon the combustion of H2, H2O is the only major by-product. In recent decades, the attractive and renewable characteristics of H2 led us to develop a variety of biological routes for the production of H2. Based on the mode of H2 generation, the biological routes for H2 production are categorized into four groups: photobiological fermentation, anaerobic fermentation, enzymatic and microbial electrolysis, and a combination of these processes. Thus, this review primarily focuses on the evaluation of the biological routes for the production of H2. In particular, we assess the efficiency and feasibility of these bioprocesses with respect to the factors that affect operations, and we delineate the limitations. Additionally, alternative options such as bioaugmentation, multiple process integration, and microbial electrolysis to improve process efficiency are discussed to address industrial-level applications.

Bioresource Technology, 2012
Remediation of real-field petroleum sludge was studied under self-induced electrogenic microenvir... more Remediation of real-field petroleum sludge was studied under self-induced electrogenic microenvironment with the function of variable organic loads (OLs) in bio-electrochemical treatment (BET) systems. Operation under various OLs documented marked influence on both electrogenic activity and remediation efficiency. Both total petroleum hydrocarbons (TPH) and its aromatic fraction documented higher removal with OL4 operation followed by OL3, OL2, OL1 and control. Self-induced biopotential and associated multiple bio-electrocatalytic reactions during BET operation facilitated biotransformation of higher ring aromatics (5-6) to lower ring aromatic (2-3) compounds. Asphaltenes and NSO fractions showed negligible removal during BET operation. Higher electrogenic activity was recorded at OL1 (343 mV; 53.11 mW/m 2 , 100 O) compared to other three OLs operation. Bioaugmentation to anodic microflora with anaerobic culture documented enhanced electrogenic activity at OL4 operation. Voltammetric profiles, Tafel analysis and VFA generation were in agreement with the observed power generation and degradation efficiency.

Dehydrogenase activity Tafel analysis Electron acceptor a b s t r a c t A novel bio-electrohydrol... more Dehydrogenase activity Tafel analysis Electron acceptor a b s t r a c t A novel bio-electrohydrolysis system (BEH) based on self-inducing electrogenic activity was designed as a pretreatment device to enhance biohydrogen (H 2 ) production efficiency of food waste. Experimental strategy involved two-stage integrated/hybrid operation with hydrolysis in initial stage followed by acidogenic fermentation for H 2 production in second stage. After pre-treatment, catabolized food waste from control (anaerobic) and BEH (closed circuit mode of operation) system was used as substrate in a separate bioreactor to evaluate H 2 production in dark-fermentation process. Pretreated-waste from BEH showed higher H 2 production (29.12 ml/h; 24th h) than control (26.75 ml/h; 16th h). Higher cumulative H 2 production and maximum substrate degradation were also noticed with BEHpretreated substrate (CHP, 0.91 l; COD, 52.42%) than control (CHP, 0.68 l; COD, 43.68%).
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Papers by Chandrasekhar Kuppam