Papers by Rodrigo Barrios
The genetic diversity contained in crops and their wild relatives is the foundation of to-morrow&... more The genetic diversity contained in crops and their wild relatives is the foundation of to-morrow's agriculture, allowing farmers and professional breeders to develop the new crop varieties that agriculture needs to adapt to changing conditions. The loss of this diversity poses a considerable risk to global food security. The conservation of agrobiodiversity in situ (in nature and in agricultural practice) as well as ex situ (in genebanks) is indispensable. However, many genebanks are unable to guarantee the safe conservation of the material in their care, let alone to create opportunities for its use in plant breeding and for more sustainable agriculture. Much remains to be done, first and foremost: Strengthening the Global System of ex situ conservation; making this System fit for purpose for hard- to-conserve materials; and innovative funding of this System.
Un plan de formación-acción para la mejora de la atención al ciudadano: el caso de la Policía Local de Castilla-La Mancha
Capital Humano Revista Para La Integracion Y Desarrollo De Los Recursos Humanos, 1999
Cell Division, 2014
Background: Heterochromatin protein 1 (HP1) is important in the establishment, propagation, and m... more Background: Heterochromatin protein 1 (HP1) is important in the establishment, propagation, and maintenance of constitutive heterochromatin, especially at the pericentromeric region. HP1 might participate in recruiting and directing Mis12 to the centromere during interphase, and HP1 disruption or abrogation might lead to the loss of Mis12 incorporation into the kinetochore. Therefore, the centromere structure and kinetochore relaxation that are promoted in the absence of Mis12 could further induce chromosome instability (CIN) by reducing the capacity of the kinetochore to anchor microtubules. The aim of this study was to determine whether alterations in the localization of HP1 proteins induced by trichostatin A (TSA) modify Mis12 and Centromere Protein A (CENP-A) recruitment to the centromere and whether changes in the expression of HP1 proteins and H3K9 methylation at centromeric chromatin increase CIN in HCT116 and WI-38 cells. Methods: HCT116 and WI-38 cells were cultured and treated with TSA to evaluate CIN after 24 and 48 h of exposure. Immunofluorescence, Western blot, ChIP, and RT-PCR assays were performed in both cell lines to evaluate the localization and abundance of HP1α/β, Mis12, and CENP-A and to evaluate chromatin modifications during interphase and mitosis, as well as after 24 and 48 h of TSA treatment. Results: Our results show that the TSA-induced reduction in heterochromatic histone marks on centromeric chromatin reduced HP1 at the centromere in the non-tumoral WI-38 cells and that this reduction was associated with cell cycle arrest and CIN. However, in HCT116 cells, HP1 proteins, together with MIS12 and CENP-A, relocated to centromeric chromatin in response to TSA treatment, even after H3K9me3 depletion in the centromeric nucleosomes. The enrichment of HP1 and the loss of H3K9me3 were associated with an increase in CIN, suggesting a response mechanism at centromeric and pericentromeric chromatin that augments the presence of HP1 proteins in those regions, possibly ensuring chromosome segregation despite serious CIN. Our results provide new insight into the epigenetic landscape of centromeric chromatin and the role of HP1 proteins in CIN.
Through a cross-cut econometric model, we try to argue to what extent the government needs to sta... more Through a cross-cut econometric model, we try to argue to what extent the government needs to stay involved in telecom investments.
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Papers by Rodrigo Barrios