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Abbas Hassani

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Papers by Abbas Hassani

Salinity Effects on the Physiological Parameters and Oxidative Enzymatic Activities of Four Iranian Grapevines (Vitis vinifera L. ) Cultivar

Soil salinity is one of the major stresses especially in arid and semi-arid regions and can limit... more Soil salinity is one of the major stresses especially in arid and semi-arid regions and can limit vine growth and productivity however responses can be different based on genotypes. In this study, effects of different concentration (0, 40, 80 and 120 Mm) NaCl on photosynthetic rate, soluble sugars, proline content and CAT activity of four own-rooted vinifera cultivars (Askari, Yaghoti, Sarghola and Rasha) were analyzed. Photosynthesis rates reduced (P �0.05) by increasing salinity, the highest and lowest reduction in photosynthesis rate was observed in Yaghoti and Rasha cultivars, respectively. Soluble sugars and proline content as an osmoregulation increased markedly at the highest NaCl concentration. Yaghoti had the highest and Rasha showed lowest soluble sugars and proline content. By increasing salt levels from 0 to 120 mM, the activity of CAT in Rasha and Sarghola cultivars increased but, Askari and Yaghoti showed no response to different NaCl concentrations. In conclusion, the...

The Effect of Different Irrigation Water Levels on Water Use Efficiency in Basil Plant (Ocimum Basilicumvar. Keshkeny Levelu) Using Marginal Analysis Theory

Journal of Medicinal Plants and By-products (2014) 2: 147-153 Original Article Effects of Arbuscular Mycorrhizal (AM) Fungi on Essential Oil Content and Nutrients Uptake in Basil under Drought Stress

Study the effects of inoculation with two arbuscular mycorrhizal (AM) fungi, Gm (Glomus mosseae T

Phenolic and flavonoid compounds and antioxidant activity in flowers of nine endemic Verbascum species from Iran

Journal of the Science of Food and Agriculture, 2021

BACKGROUND In this study, flowers of nine mullein species (Verbascum erianthum, V. songaricum, V.... more BACKGROUND In this study, flowers of nine mullein species (Verbascum erianthum, V. songaricum, V. speciosum, V. szovitsianum, V. stachydiforme, V. sinuatum, V. haussknechtianum, V. cheirantifolium, and V. saccatum) were collected from Iran. Total flavonoid content (TFC), antioxidant activity, total carotenoid (TCC) and β-carotene content and the amount of phenolic compounds were determined in all of the collected mullein species. Quantitative analysis of phenolic compounds was performed by using HPLC-DAD. RESULTS V. saccatum and V. songaricum, in particular, were found rich in total flavonoid so exhibit the highest antioxidant capacity. The HPLC-DAD analysis revealed that V. erianthum possesses the highest amount of caffeic acid (0.022 g kg-1 DW), chlorogenic acid (2.649 g kg-1 DW), p-coumaric acid (0.253 g kg-1 DW), and apigenin (0.066 g kg-1 DW) and the highest amount of gallic acid (0.134 g kg-1 DW), rutin (5.254 g kg-1 DW), quercetin (1.303 g kg-1 DW), and cinnamic acid (1.031 g kg-1 DW) content were observed in V. saccatum. Our results demonstrate the considerable variations in the TFC, antioxidant activity, TCC and β-carotene content among the mullein species. CONCLUSIONS The findings of this study provide remarkable information for schematization breeding strategies, and for choosing the best species with high phenolic compounds content to produce natural antioxidant compounds as a medicinal plant for the medical uses and pharmaceutical industries. This article is protected by copyright. All rights reserved.

High-Frequency in Vitro Direct Shoot Regeneration from Nodal Explants of Hyssop Plant (Hyssopus officinalis L.)

Journal of Medicinal plants and By-product, Sep 1, 2016

Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass producti... more Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass production through in vitro culture, two individual experiments was conducted. Effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of TDZ and BAP in combination with 1 µmol of IAA on direct regeneration from nodal explants were assessed. Significant difference between treatments was observed (P≤ 0.01). In BAP treatments, the maximum shoot-buds induction (9 shoot-buds per explant) and shoot regeneration percentage (96.66%) were observed on MS medium fortified with 2.2 and 4.4 µmol BAP in combination with 1 µmol of IAA. In TDZ treatments, the highest regeneration percentage was achieved in MS medium supplemented with TDZ (2.2 µmol) and IAA (1 µmol), and the maximum shoot-buds induction (19.83 shoot-buds per explant) was observed in medium containing 4.4 µmol of TDZ in combination with 1 µmol of IAA. The highest root production frequency (89.5%) was achieved in medium contained 9.84 µmol of IBA. Rooted plants were acclimatized successfully in greenhouse conditions with 100% survival. The protocol described here could be applicable for mass in vitro production of the valuable medicinal plant Hyssopus officinalis L. for its genetic resource conservation as well as pharmaceutical purpose.

Genetic analysis of castor (Ricinus communis L.) using ISSR markers

Castor (Ricinus communis L.) is one of the most ancient medicinal oil crops in the world. It has ... more Castor (Ricinus communis L.) is one of the most ancient medicinal oil crops in the world. It has been vastly distributed in different parts of Iran. In the present study, the inter simple sequence repeat (ISSR) markers were used to evaluate the molecular genetic diversity among and within 12 castor accessions collected from 6 regions of Iran. Totally, 16 ISSR primers amplified 166 loci, of which 116 loci (69.89 %) were polymorph, indicating high genetic variability in castor germplasm. An accession-specific ISSR band was detected in ‘80-29’accession. Genetic distance among accessions ranged from 0.2 to 0.056. Analysis of molecular variance revealed a higher level of genetic variation within (80%) than between (20%) accessions. A model-based Bayesian approach subdivided 60 genotypes from 12 accessions into 6 subgroups. UPGMA dendrogram based on Nei’s genetic distance classified 12 accessions into 4 groups. The result indicates that there was no association between geographical origin...

Qualitative and Quantitative Changes in the Essential Oil of Origanum vulgare ssp. gracile as Affected by Different Harvesting Times

Qualitative and quantitative variations in the essential oil of wild growing Origanum vulgare L. ... more Qualitative and quantitative variations in the essential oil of wild growing Origanum vulgare L. ssp. gracile plants were studied in response to different phenological stages (pre, full and post-flowering). The essential oil of air-dried leaves was isolated by water distillation using a Clevenger-type apparatus and was analyzed by Gas Chromatography (GC) and Gas Chromatography–Mass Spectrometry (GC/MS). The highest (1.87%) and the lowest (1.01%) essential oil content were obtained from post-flowering and preflowering stages, respectively. In total, 24 components were identified and quantified in three phenological stages representing 96.75, 97.63, and 98.59% of the oil, respectively. Carvacrol (46.62, 46.5 and 27.6%), ρ-cymene (7.76, 13.54 and 37.08%) and γ-terpinene (21.54, 13.91 and 6.82%) were the main constituents of essential oils in pre, full, and postflowering stages, respectively. Oxygenated monoterpenes (43.35-61.32%) and monoterpene hydrocarbons (30.81-48.02%) were the mai...

Phenology of German Chamomile and its Changes under Different Irrigation Regimes and Plant Densities

Notulae Scientia Biologicae, 2010

In order to definite growth stages of Matricaria chamomilla L., 40 plants were planted with 200 ×... more In order to definite growth stages of Matricaria chamomilla L., 40 plants were planted with 200 × 200 cm distance from each other. To determine the phenology under different water stress condition and plant densities, an experiment was conducted in factorial based on randomized complete block design with two factors including irrigation at 4 levels (25, 50, 75, and 100 mm evaporation from pan class A), and plant density at 5 levels (cultivation in 30 cm rows with 5, 10, 15, 20 and 25 cm intra-row spaces) with three replications.

Allelopathy of Sage and White Wormwood on Purslane Germination and Seedling Growth

Notulae Scientia Biologicae, 2010

A bioassay run was carried out in the incubator to evaluate possible allelopathic effects of wate... more A bioassay run was carried out in the incubator to evaluate possible allelopathic effects of water extracts of sage and white wormwood on germination and seedling growth of purslane. Results showed that the type of extract and its concentration (0, 5, 10, 15 and 20%) significantly influenced final germination percentage, germination rate and index, root and shoot length, root/shoot ratio, fresh and dry weight of seedling of purslane. The interaction between these two experimental factors was always significant, producing different results according to the different combination levels. The statistical comparison of means indicated that the maximum germination percentage and germination rate (respectively 68% and 11.4% d-1) were obtained from the untreated control (0% extract), while the lowest values for the same two characters occurred with 15% of sage (respectively 45% and 6.4% d-1) and 20% of white wormwood (38% and 7.5% d-1). Concerning germination indexes, the highest (55) and t...

Thymus kotschyanus and Carum copticum essential oils as botanical preservatives for table grape

Journal of Medicinal Plants Research, Nov 18, 2010

In the present study, essential oils extracted from Thymus kotschyanus and Carum copticum has bee... more In the present study, essential oils extracted from Thymus kotschyanus and Carum copticum has been evaluated for efficacy in the control of postharvest fungal decay in Thompson seedless table grape. Results showed that the tested oils exhibited good inhibitory activity against fungal decay in oil-treated grapes. In addition, the essential did not show a negative impact on the sensory quality of the grape. The profile of the oil components showed that carvacrol (28.54%) and thymol (63.18%) were the main compounds available in T. kotschyanus and C. copticum respectively. Results of this research showed that the evaluated essential oils may be used as a preservative for reduction of postharvest losses.

Effects of Irrigation Regime and Plant Density on Essential Oil Composition of German Chamomile (Matricaria chamomilla)

Journal of Herbs, Spices & Medicinal Plants, 2011

A two-year factorial experiment was conducted in Randomized Complete Block Design to evaluate the... more A two-year factorial experiment was conducted in Randomized Complete Block Design to evaluate the effect of irrigation regimes on the yield of biomass, dried flower, essential oil and seed, and harvest index of dried flower, essential oil and seeds of German chamomile at different plant densities. Factors applied were irrigation regimes (irrigation at 25, 50, 75, and 100 mm evaporation from pan class A) and plant density (5, 10, 15, 20 and 25 cm intra-row spaces with 30 cm inter-row space) with three replications. Results showed the significant effect of irrigation and plant density on the yield and harvest index of dried flower, essential oil and biomass. The highest yield of dried flower (1,136 kg/ha) was obtained from irrigation at 50 mm evaporation from pan, followed by 75, 25 and 100 mm evaporation, respectively. The highest (7,474 g/ha) and lowest (5,812 g/ha) essential oil yield were obtained from irrigation at 50 and 100 mm evaporation, respectively. The greatest biomass (2,989 kg/ha) observed at 25 mm evaporation, as well as irrigation at 50 and 75 mm and the minimum (1,882kg/ha) was at 100 mm. The highest yield of dried flower (1,241 kg/ha), essential oil (8,057 g/ha), seed (765 kg/ha) and biomass (2,716kg/ha) were obtained from 10 cm intra-row distance, whereas the lowest yield of dried flower (765 kg/ha), essential oil (4,921 g/ha) and seed (574 kg/ha) observed at 25 cm distance. The minimum biomass (1,768 kg/ha) was obtained from 5 cm intra-row space. The maximum (39%) and minimum (33%) harvest index of dried flower were obtained from irrigation at 100 and 25 mm evaporation, while the highest (47%) and lowest (29%) harvest index belonged to 5 and 25 cm planting spaces, respectively. The largest and smallest harvest index of essential oil concerned to 5 (0.324%) and 25 cm (0.192%), respectively. Our finding showed that the best irrigation was irrigation at 50 mm evaporation from pan and the optimal plant distance was 10 cm intra-row space.

Essential Oils as Control Agents of Postaharvestalternariaandpenicilliumrots on Tomato Fruits

Journal of Food Safety, 2010

The antifungal activity of the essential oils of ajowan, fennel and caraway was evaluated in vitr... more The antifungal activity of the essential oils of ajowan, fennel and caraway was evaluated in vitro and in vivo conditions against growth of Alternaria alternata (Fr.: Fr.) Keissler. and Penicillium digitatum Sacc., two postharvest pathogens of tomato fruits. In vitro tests showed that ajowan and fennel oils exhibited the highest antifungal activity against A. alternata and P. digitatum, respectively. But caraway exhibited poor antifungal activity against two fungal species tested. Ajowan oil possessed the highest antifungal activity among tested oils in in vivo condition. Gas chromatography-mass spectrometry analysis of essential oils revealed that thymol (63%), trans-anethole (64%) and 2-caren-10-al (34%) were the main components of essential oils of

Chemical characterization of bioactive volatile molecules of four thymus species using nanoscale injection method

Digest Journal of Nanomaterials and Biostructures, 2009

The genus Thymus L. is a well known aromatic perennial herb originated from Mediterranean region.... more The genus Thymus L. is a well known aromatic perennial herb originated from Mediterranean region. Among 215 species of this genus grown in the world, 14 species are distributed in Iranian flora [1-3]. Thymus species are well known as medicinal plants because of their biological and pharmacological properties. In traditional medicine, the leaves and flowering parts of Thymus species are widely used as tonic and herbal tea, flavouring agents (condiment and spice), antiseptic, antitussive and carminative as well as ...

Analysis of Morphological and Molecular Genetic Diversity of Salicornia Iranica Akhani Wildlife Populations around Urmia Lake, Iran

Salicornia is a halophyte plant capable of being irrigated with seawater, which can be used as an... more Salicornia is a halophyte plant capable of being irrigated with seawater, which can be used as an alternative food. Given this, it is necessary to study the potentials of this plant's morphological diversity in the natural environment. In this study, 33 wild populations of Salicornia were collected from different geographical areas around Urmia Lake during the flowering stage, and some morphological traits and 25 ISSR loci of the plant were measured. Based on morphological traits and the cluster analysis, Salicornia populations were divided into four groups. Overall, the high percentage of polymorphic gene loci (65.69%), the average number of effective alleles per gene locus (1.63), and the Shannon data index (0.540) indicate that ISSR markers can be used in Identify genetic diversity to be used. Molecular data cluster analysis divided the studied populations into two main groups, which included 12.12% and 87.88% of the populations, respectively. Based on the effective analysis ...

A Study of Islamic and Conventional Banks in Malaysia

Journal of King Abdulaziz University-Islamic Economics

Many scholars have researched comparison of conventional and Islamic banking systems, with regard... more Many scholars have researched comparison of conventional and Islamic banking systems, with regards to their performance from time to time. This study compares their respective profitability in terms of volume and volatility of their deposits and loans in Malaysia in a six years period (2006-2011) that other writers have not discussed in detail. The results in this study indicate that there are positive and significant relationships between volume of loans and volume of customers' deposits and profitability of banks. However, volatility of loans and deposits does not have a significant impact on banks' profit-efficiency in Malaysia. The results of this research also reveal that Malaysian conventional banks were more profitable than Islamic banks during the period of study. The ROA and ROE are significantly different and mean of ROA and ROE in conventional banks are higher than those in Islamic banks. The main contribution of banks' profits comes from the spread between the interest earned on loans and the return payable on customer deposits. Both of these are internal factors. Volume of loans and volume of deposits are found to have significant influence on banks' profitability. Therefore, managers and banks' administrators should either focus their attention on these two determinants or adopt some kind of policies that will lead banks to increase their volume of loans and deposits to boost banks profitability level.

Seasonal variations of essential oil content and composition in male and female plants of Juniperus communis L. ssp. hemisphaerica growing wild in Iran

Journal of Essential Oil Research

Effects of irrigation intervals and organic manure on morphological traits, essential oil content and yield of oregano ( Origanum vulgare L.)

Anais da Academia Brasileira de Ciencias

In order to evaluate the effect of irrigation intervals and cattle manure levels on morphological... more In order to evaluate the effect of irrigation intervals and cattle manure levels on morphological traits, essential oil content and yield of oregano, an experiment was conducted at the experimental Farm of the Faculty of Agriculture, Urmia University, Iran. The experimental design was split-plots, arranged in randomized complete blocks with three replications. Main plots including irrigation intervals (1, 2 and 3 weeks) and four levels of cattle manure at 0, 10, 20 and 30 t ha-1 were allocated to sub plots. Our results showed that increasing irrigation intervals reduced values of all morphological traits except for proportion of stems. Also, values for stems number, plant spread, stem diameter, leaf area, fresh and dry herb yield increased by increasing cattle manure levels. On the other hand, morphological traits not influenced by interaction of treatments except for plant spread and leaf area. The highest essential oil content (2.07%) and yield (66.62 kg ha-1) obtained in highest ...

Effects of salinity on leaf mineral composition and salt injury symptoms of some Iranian wild grapevine (<em>Vitis vinifera</em> L. ssp. sylvestris) genotypes

OENO One, 2014

To evaluate the suitability of wild grapevine genotypes to saline conditions by measuring leaf io... more To evaluate the suitability of wild grapevine genotypes to saline conditions by measuring leaf ion content and salt injury symptoms. Methods and results: Vines of nine wild (Vitis vinifera L. ssp. sylvestris) genotypes were planted in pots and after the good establishment, salinity treatments (0, 50, 100 and 150 mM NaCl) started. At the end of the experimental period, the K + , Na + , NO 3 and Clcontent of leaves and visible symptoms of salt injury were recorded. Leaf Na + and Clcontent increased with increasing salinity but levels and accumulation rates were different among genotypes. Conclusion : Based on Na + and Clcontent and salt symptoms, genotypes 4 and 7 showed less symptoms than the other genotypes at moderate (50-100 mM) NaCl concentration and none could tolerate high salt (150 mM) concentration. Significance and impact of the study : Under saline conditions, ion accumulation in the leaves significantly varied among wild genotypes and some of them could be recommended as saline tolerant genotypes.

Influences of various factors on hairy root induction in Agastache foeniculum (Pursh) Kuntze

Acta agriculturae Slovenica, 2016

Agrobacterium rhizogenes is known as a natural tool of genetic engineering in many plant species.... more Agrobacterium rhizogenes is known as a natural tool of genetic engineering in many plant species. For the first time, hairy root induction in Agastache foeniculum using A. rhizogenes, rosmarinic acid content and the effect of different culture media and inoculation methods on hairy root growth rate were investigated. Hairy root culture of A. foeniculum was established by inoculation of the 1-month-old leaf explant with A4 strain of A. rhizogenes and the effectiveness of lightdark conditions and two inoculation methods (immersion and injection) were tested. Furthermore, in immersion method, the effects of inoculation time (3, 5 and 7 min) on root induction were investigated. In the second part of the study, the hairy root culture of A. foeniculum was studied using different basal culture media (MS, 1/2 MS and B5). Rosmarinic acid content in hairy roots and non-transformed roots was analyzed using high-performance liquid chromatography (HPLC). There was no significant difference between various inoculation methods in the ability of hairy roots induction. Observations showed that percentage of hairy root induction was higher when the explants were immersed for 5 min in bacterial suspension. Light conditions displayed the highest hairy root induction rates compared with dark condition. Various culture media are different in terms of types and amounts of nutrients and have influence on growth rate. The maximum growth rate (1.61 g fr wt/50 ml) of hairy roots were obtained in 1/2 MS medium. Rosmarinic acid content in transformed roots (213.42 µg/g dry wt) was significantly higher than non-transformed roots (52.28 µg/ g dry wt).

Salicylic Acid and UV-B/C Radiation Effects on Growth and Physiological Traits of <i>Satureja hortensis</i> L

Notulae Scientia Biologicae, 2016

Higher UV irradiation may affect the structure of ecosystems directly or indirectly. In the prese... more Higher UV irradiation may affect the structure of ecosystems directly or indirectly. In the present work, Satureja hortensis L. defence mechanisms against UV stress was studied with or without salicylic acid (SA). For this aim, a factorial based experimental was conducted on completely randomized design with three UV treatments (control, UV-B and UV-C) as first factor and two SA levels (0 and 1 mM) as second factor. The results showed UV-B and C decreased shoot dry weight, plant height (PH), node number (NN), inter-node distance (IND), root length (RL), leaf area index (LAI) and chlorophyll content, but increased stem diameter (SD), leaf thickness (LTh), total flavonoid content (TFC), total phenolic content (TFC) and antioxidant activity. Plants treated with SA (1 mM) showed higher growth factors than non-treated one. It seems that SA can be used as an alternative substance against UV stress.