Papers by Nevcivan Guldas
DergiPark (Istanbul University), Aug 1, 2010
Bir projenin parçası olan bu çalışmanın ön verileri 2-4 Eylül 2007 tarihlerinde İzmir'de düzenlen... more Bir projenin parçası olan bu çalışmanın ön verileri 2-4 Eylül 2007 tarihlerinde İzmir'de düzenlenen II. Ulusal Sağlık Hizmetleri Meslek Yüksekokulları Sempozyumu'nda bildiri olarak sunulmuştur. Sağlık sektörünün tıbbi laboratuar teknikerinden beklentileri 46 according to communities needs and sector's expectations. This study was aimed to define the necessary knowledge and skills according to the expectations of the sector, and to formulate the gaps between its theoretical and practical parts. Material and method: The study frame consisted of 366 health care institutions wich employ MLTs and located within 8 districts of the Metopolitan City of İzmir. Sample size was defined as 85 institutions. Sector representative's (SP) expectation were investigated by a questionair form which was developped according to national occupational standards and curriculum contents. Structural charecteristics of the institutions were also examined. Questionaires were distributed to health institutions representatives and collected back by study asistants. Sector representatives expectations were studied under three headings: Fundamental Medical Knowledge (FMN), Supportive Knowledge and Skills (SKS), Fundamental Occupational Knowledge and Skills (FOKS). Results: Access rate was 86%. SK's expectations under FMN, SKS and FOKS were transformed into quantitative form in order to facilitate the comparisons and the figures were calculated respectively as 60.4 ± 15.2; 75.5 ± 15.2; 83.1± 11.1. Outpatient clinics, institutions which employ less then four MLTs and private sector institutions' expectations under FMN were higher (respectively 0,0001, 0,01 ve 0.01) than others. Conclusion: This study presents a set of important data which would be useful in the process of defining insufficiencies of curriculum and gaps between knowledge and skills.
Mikrobiyoloji Bulteni, Jan 24, 2013
Staphylococcus aureus suşlarının neden olduğu enfeksiyonların görülme sıklığı ve buna bağlı olara... more Staphylococcus aureus suşlarının neden olduğu enfeksiyonların görülme sıklığı ve buna bağlı olarak antibiyotik direnci giderek artmaktadır. Bu nedenle hem hastane hem de toplum kaynaklı S.aureus enfeksiyonlarının tedavisinde ciddi problemler yaşanmaktadır. Bu çalışmada, gentamisinin MİK ve sub-MİK dilüsyonlarının S.aureus'un virülans faktörlerinden biyofilm ve koagülaz oluşumuna etkisinin in vitro olarak ve hücre kültür ortamında belirlenmesi amaçlanmıştır. Çalışmaya standart S.aureus ATCC 25923 suşu ile kan kültürlerinden izole edilen iki klinik S.aureus suşu (K1 ve K2) alınmış; suşların gentamisin MİK değerleri CLSI standartlarına uygun olarak katyon ilaveli Mueller Hinton buyyonunda mikrodilüsyon yöntemi ile araştırılmıştır. Her suş için gentamisin MİK, %50 MİK ve %25 MİK değerleri ayrı ayrı belirlenmiştir. Belirlenen MİK yoğunluklarında suşların biyofilm oluşturma özelliği, kristal viyole boyama yöntemi ile spektrofotometrik olarak saptanmış; koagülaz aktiviteleri ise tüpte değerlendirilmiştir. İnsan kökenli epitelyal hücre kültürleri olan HEp-2 hücre dizileri, standart ve klinik S.aureus suşları ile enfekte edildikten sonra (Multiplicity of infection: 50/1) iki saat inkübasyona bırakılmıştır. Enfekte edilen hücre dizilerine her suş için ayrı ayrı belirlenen gentamisinin MİK, %50 MİK ve %25 MİK yoğunlukları eklenerek 18 saat inkübe edilmiş; daha sonra hücreler distile su ile patlatılarak açığa çıkan bakterilerin biyofilm ve koagülaz üretimleri değerlendirilmiştir. Suşların hücre ile karşılaşmadan önceki ve sonraki biyofilm oluşumları değerlendirildiğinde S.aureus ATCC 25923 ve K1 suşlarında bir değişiklik gözlenmezken, K2
Journal of basic and clinical health sciences, Jan 31, 2023
world, a pandemic on March 11, 2020, due to causing thousands of deaths and a high infection rate... more world, a pandemic on March 11, 2020, due to causing thousands of deaths and a high infection rate (2). Although SARS-CoV-2 is genomically similar to other members of the coronavirus family, such as the SARS-CoV and MERS-CoV virus, which have caused epidemics in various countries around the ABSTRACT Purpose: COVID-19 is an important public health problem impacting the whole world. This study aimed to evaluate health technician students' knowledge and behaviors regarding COVID-19 and protective measures. Material and Methods: This cross-sectional study was performed with Dokuz Eylül University Vocational School of Health Services students (n=648). Data were collected online using a descriptive data registration form that consisted of 65 questions via Google Forms. Results While the students' mean knowledge score on COVID-19 and protective measures was 15.31±3.22, the mean behavior score was 10.53±3.34. A significant difference was detected between the students' education program, gender, father's educational status, smoking, and exercise habits and total behavior score averages related to COVID-19 and protective measures (p<0.05). Students who received training on the prevention of infectious diseases during their student years and who stated that they received any training on COVID-19 had higher mean knowledge and behavior scores regarding protective measures against COVID-19 (p<0.05). Students who were afraid of being infected with COVID-19, who had had COVID-19, and whose relatives died due to COVID-19 had higher mean behavior scores regarding protective measures against COVID-19 (p<0.05). Conclusion: It was revealed that the students participating in the study had moderate knowledge about COVID-19 and protective measures and exhibited positive behavior regarding protective measures. Since increasing the knowledge levels of future health technicians will also be reflected in their protective behaviors, which are important for their professional life, it is recommended that necessary arrangements be made in the education curriculum to improve their knowledge and behavior.
Journal of basic and clinical health sciences, 2020
Objective: The aim of the study is to apply a risk management model for the examination and preve... more Objective: The aim of the study is to apply a risk management model for the examination and prevention of errors occurring in the preanalytical laboratory phase. Method: The data used in this study were obtained from the Error Reporting Sheet of Dokuz Eylul University Hospital Central Laboratory for 2013-2015. The Failure Mode and Effects Analysis (FMEA) method was used in the study. The pre-analytical laboratory process was defined and FMEA team was formed. By determining the potential effects of failure modes detected in the pre-analytical process, the severity, probability and detectability of them were calculated and potential causes were identified. An action plan was prepared to eliminate or reduce the highrisk failure modes. Results: In the pre-analytical laboratory phase, processes are defined as test request, sampling, identification, barcoding and transfer. The errors that occurred in the pre-analytical phase were classified under 9 types of errors. The highest number of errors was observed in the patient identification process and the number and the ratio of total errors in 2013 are 71 (29.58%), in 2014 it is 81 (29.89%) and in 2015 it is 102 (53.97%). It was observed that the failure mode with the highest risk priority number value with 576 points in the pre-analytical phase occurred in the identification process. Conclusion: With the FMEA procedure that can be included in the laboratory's quality system, it is expected to minimize the possibility of errors in the pre-analytical phase and improve the quality of laboratory tests.
Klimik Dergisi, Mar 13, 2019
Objective: Healthcare workers' mobile phones can be colonized by bacteria known to cause nosocomi... more Objective: Healthcare workers' mobile phones can be colonized by bacteria known to cause nosocomial infections. The aim of this study is to determine contamination of mobile phones of health care workers in the intensive care units and the operating room and the risk factors related to microbial contamination. Methods: A total of 96 culture samples were taken from mobile phones of health care workers who work in the Anesthesia, Internal Medicine and Pediatric Intensive Care Units and the Operating Room. A questionnaire about mobile phone usage habits was administered. The samples were screened for bacterial pathogens by standard bacteriological procedures. The methicillin sensitivity of Staphylococcus aureus isolates was tested. Results: The rate of bacterial contamination of mobile phones of health care workers was 90.6%. There was no relationship between gender, age and occupation of healthcare workers and mobile phone bacterial contamination (p value 0.270, 0.796, 0.414, respectively). In the Pediatric Intensive Care Unit, the rate of bacterial contamination was significantly lower than the other departments (p<0.001). Coagulase-negative staphylococci (57%) were the most frequently isolated bacteria followed by Bacillus spp. (32%), methicillin-sensitive S. aureus (5%), Pseudomonas stutzeri (2%), P. aeruginosa (1%), Stenotrophomonas maltophilia (1%), Sphingomonas paucimobilis (1%) and Aeromonas hydrophila/caviae (1%). Conclusions: It has been concluded that mobile phones of healthcare workers are contaminated with microorganisms which may be the cause of nosocomial infection. In order to increase awareness of healthcare workers about the possible risks of using mobile phones in the hospital environment, "mobile phone usage rules" and "mobile phone decontamination" methods should be added to infection control measures and the effectiveness of these measures should be evaluated.
Journal of basic and clinical health sciences, Mar 4, 2021
Staphylococcus aureus colonized in the nose of healthcare workers is an important risk factor for... more Staphylococcus aureus colonized in the nose of healthcare workers is an important risk factor for the development of hospital-acquired staphylococcal infections. Cross-contamination of this bacterium between the hands of healthcare workers and the surfaces they contact is known. In this study, we aimed to evaluate the clonal relationship between eight S. aureus strains isolated from the nose of healthcare providers and five S. aureus strains isolated from mobile phones carried by healthcare providers. Methods: The clonal relationship between the strains and molecular epidemiological status were investigated by the pulsed-field gel electrophoresis (PFGE) method. Results: The first and third strains are isolated from the mobile phone and the nose of a healthcare provider working in the intensive care unit were the same. The second and fourth strains were isolated from the mobile phone and nose of another healthcare provider working in the intensive care unit were the same. The fifth strain, which was found to be the same as the second and fourth strains, was isolated from the mobile phone of another healthcare provider working in the intensive care unit. No similarity was observed between the other strains. Conclusion: Our findings indicate that S. aureus strains colonized in the nose of healthcare workers are also transmitted to other surfaces and that the hospital environment and co-used devices pose a risk for spread. For this reason, training of healthcare workers on the infection control procedure, hand hygiene, environmental disinfection and regular cleaning of mobile phones are important components in order to prevent hospital-acquired infections.
Health Promotion Journal of Australia, 2020
Issue addressedThe aim of this study was to determine the knowledge and behaviour of health techn... more Issue addressedThe aim of this study was to determine the knowledge and behaviour of health technician students to determine their oral health (OH) status, and to evaluate the effectiveness of a structured education administered for increasing OH awareness.MethodsInterventional research was performed between December 2018 and March 2019 on 445 Vocational School of Health Services students in … University. Questionnaires were conducted under observation. The first questionnaire (Q1) included socio‐demographic characteristics, OH knowledge level, attitudes and behaviours. One month after the OH training was given to the students, the same questions in Q1 were asked again in the second questionnaire (Q2). Dental caries and Simplified Oral Hygiene Index (OHI‐S) status were determined by a dentist via a clinical oral examination.ResultsOf the 445 students, 416 (93.5%) participated in the study and completed the Q1 and Q2 questionnaires. A total of 277 (66.6%) students were female and 139...
Infection, 2004
Background: Although hepatitis B virus (HBV) infections are a major health problem in Turkey, the... more Background: Although hepatitis B virus (HBV) infections are a major health problem in Turkey, there is little information on the genotype distribution of the virus. In this study, HBV genotypes were determined by DNA sequencing and restriction enzyme analysis of the S gene. Materials and Methods: The S genes of hepatitis B virus isolated from 23 chronically infected HBV-DNA-positive Turkish patients were amplified and directly sequenced. Nucleotide sequences were then aligned with reference isolates from the GenBank database and subjected to phylogenetic analysis. HBsAg subtype-specific aminoacid substitutions at codons 122, 127, 134, 159, and 160 were analyzed using translated sequences. The amplified products were also subjected to restriction enzyme analysis using endonucleases Mva I, Rsa I, and Hinf I. Results: Phylogenetic analysis showed clustering of all samples with genotype D sequences. The mean intragroup divergence was 0.95% (range 0.00%-4.00%). All isolates were ayw2, as predicted from translated sequences. The results of the restriction enzyme analysis of the samples were consistent with the phylogenetic analysis. Conclusion: Our data complement the findings that genotype D viruses are prevalent in the Turkish population. Being rapid and inexpensive, restriction enzyme analysis described in this study should be useful for large-scale epidemiological analysis of HBV infections.
The Journal of Basic and Clinical Health Sciences, 2019
Objectives: Staphylococcus aureus (S. aureus) is one of the major human pathogens in both communi... more Objectives: Staphylococcus aureus (S. aureus) is one of the major human pathogens in both community acquired and nosocomial infections. Heavy increase of antibiotic resistance between S. aureus strains became an important public health problem in progress of time. In this study, the antimicrobial effects of piceatannol on S. aureus growth was investigated. Patients and Methods: The antimicrobial effect of piceatannol on a standard S. aureus (DSMZ 6148) strain and two clinical S. aureus strains (C1 and C2) was tested in vitro at concentrations between 0 and 750 µg/ mL. Tigecycline and gentamicin were used as positive controls. For each strain, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of piceatannol and the control antibiotics were determined separately using the broth microdilution method according to CLSI (Clinical and Laboratory Standards Institute) standards at 24 and 48 h. Results: After 24 and 48 h of treatment with piceatannol, the average MIC for all tested strains was 283 µg/mL and 383 µg/mL, respectively. Bactericidal activity increased as piceatannol concentration increased for one of the three strains. After 24 and 48 h of treatment with piceatannol, the average MBC for all strains was 717 µg/ mL and 583 µg/ mL, respectively. The S. aureus strains were found to be susceptible to tigecycline and gentamicin. Conclusion: Piceatannol has antimicrobial effect against S. aureus; however, more data regarding the effects of this compound on other microorganisms and its bioavailability are needed.
Staphylococcus aureus colonized in the nose of healthcare workers is an important risk factor for... more Staphylococcus aureus colonized in the nose of healthcare workers is an important risk factor for the development of hospital-acquired staphylococcal infections. Cross-contamination of this bacterium between the hands of healthcare workers and the surfaces they contact is known. In this study, we aimed to evaluate the clonal relationship between eight S. aureus strains isolated from the nose of healthcare providers and five S. aureus strains isolated from mobile phones carried by healthcare providers. Methods: The clonal relationship between the strains and molecular epidemiological status were investigated by the pulsed-field gel electrophoresis (PFGE) method. Results: The first and third strains are isolated from the mobile phone and the nose of a healthcare provider working in the intensive care unit were the same. The second and fourth strains were isolated from the mobile phone and nose of another healthcare provider working in the intensive care unit were the same. The fifth strain, which was found to be the same as the second and fourth strains, was isolated from the mobile phone of another healthcare provider working in the intensive care unit. No similarity was observed between the other strains. Conclusion: Our findings indicate that S. aureus strains colonized in the nose of healthcare workers are also transmitted to other surfaces and that the hospital environment and co-used devices pose a risk for spread. For this reason, training of healthcare workers on the infection control procedure, hand hygiene, environmental disinfection and regular cleaning of mobile phones are important components in order to prevent hospital-acquired infections.
Pro- inflammatory Effects of Carvacrol on Staphylococcus aureus
Mikrobiyoloji Bulteni, 2013
Staphylococcus aureus suşlarının neden olduğu enfeksiyonların görülme sıklığı ve buna bağlı olara... more Staphylococcus aureus suşlarının neden olduğu enfeksiyonların görülme sıklığı ve buna bağlı olarak antibiyotik direnci giderek artmaktadır. Bu nedenle hem hastane hem de toplum kaynaklı S.aureus enfeksiyonlarının tedavisinde ciddi problemler yaşanmaktadır. Bu çalışmada, gentamisinin MİK ve sub-MİK dilüsyonlarının S.aureus'un virülans faktörlerinden biyofilm ve koagülaz oluşumuna etkisinin in vitro olarak ve hücre kültür ortamında belirlenmesi amaçlanmıştır. Çalışmaya standart S.aureus ATCC 25923 suşu ile kan kültürlerinden izole edilen iki klinik S.aureus suşu (K1 ve K2) alınmış; suşların gentamisin MİK değerleri CLSI standartlarına uygun olarak katyon ilaveli Mueller Hinton buyyonunda mikrodilüsyon yöntemi ile araştırılmıştır. Her suş için gentamisin MİK, %50 MİK ve %25 MİK değerleri ayrı ayrı belirlenmiştir. Belirlenen MİK yoğunluklarında suşların biyofilm oluşturma özelliği, kristal viyole boyama yöntemi ile spektrofotometrik olarak saptanmış; koagülaz aktiviteleri ise tüpte değerlendirilmiştir. İnsan kökenli epitelyal hücre kültürleri olan HEp-2 hücre dizileri, standart ve klinik S.aureus suşları ile enfekte edildikten sonra (Multiplicity of infection: 50/1) iki saat inkübasyona bırakılmıştır. Enfekte edilen hücre dizilerine her suş için ayrı ayrı belirlenen gentamisinin MİK, %50 MİK ve %25 MİK yoğunlukları eklenerek 18 saat inkübe edilmiş; daha sonra hücreler distile su ile patlatılarak açığa çıkan bakterilerin biyofilm ve koagülaz üretimleri değerlendirilmiştir. Suşların hücre ile karşılaşmadan önceki ve sonraki biyofilm oluşumları değerlendirildiğinde S.aureus ATCC 25923 ve K1 suşlarında bir değişiklik gözlenmezken, K2
Klimik Dergisi/Klimik Journal
Objective: Healthcare workers' mobile phones can be colonized by bacteria known to cause nosocomi... more Objective: Healthcare workers' mobile phones can be colonized by bacteria known to cause nosocomial infections. The aim of this study is to determine contamination of mobile phones of health care workers in the intensive care units and the operating room and the risk factors related to microbial contamination. Methods: A total of 96 culture samples were taken from mobile phones of health care workers who work in the Anesthesia, Internal Medicine and Pediatric Intensive Care Units and the Operating Room. A questionnaire about mobile phone usage habits was administered. The samples were screened for bacterial pathogens by standard bacteriological procedures. The methicillin sensitivity of Staphylococcus aureus isolates was tested. Results: The rate of bacterial contamination of mobile phones of health care workers was 90.6%. There was no relationship between gender, age and occupation of healthcare workers and mobile phone bacterial contamination (p value 0.270, 0.796, 0.414, respectively). In the Pediatric Intensive Care Unit, the rate of bacterial contamination was significantly lower than the other departments (p<0.001). Coagulase-negative staphylococci (57%) were the most frequently isolated bacteria followed by Bacillus spp. (32%), methicillin-sensitive S. aureus (5%), Pseudomonas stutzeri (2%), P. aeruginosa (1%), Stenotrophomonas maltophilia (1%), Sphingomonas paucimobilis (1%) and Aeromonas hydrophila/caviae (1%). Conclusions: It has been concluded that mobile phones of healthcare workers are contaminated with microorganisms which may be the cause of nosocomial infection. In order to increase awareness of healthcare workers about the possible risks of using mobile phones in the hospital environment, "mobile phone usage rules" and "mobile phone decontamination" methods should be added to infection control measures and the effectiveness of these measures should be evaluated.
Nasal carriage of Staphylococcus aureus and presence of genetically identical strains on the mobile phones carried by healthcare providers in the intensive care unit
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Papers by Nevcivan Guldas