Human lymphocytes were either exposed to X-irradiation (25 to 200 rads) or treated with HzOz (9.1... more Human lymphocytes were either exposed to X-irradiation (25 to 200 rads) or treated with HzOz (9.1 to 291 u&f) at 4°C and the extent of DNA migration was measured using a singlecell microgel electrophoresis technique under alkaline conditions. Both agents induced a significant increase in DNA migration, beginning at the lowest dose evaluated. Migration patterns were relatively homogeneous among cells exposed to X-rays but heterogeneous among cells treated with HzOz. An analysis of repair kinetics following exposure to 200 rads X-rays was conducted with lymphocytes obtained from three individuals. The bulk of the DNA repair occurred within the first IS min, while all of the repair was essentially complete by 120 min after exposure. However, some cells demonstrated no repair during this incubation period while other cells demonstrated DNA migration patterns indicative of more damage than that induced by the initial irradiation with X-rays. This technique appears to be sensitive and useful for detecting damage and repair in single cells. @ IYXX Academic Press. Inc.
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