Papers by John P Délano-Frier
Real-time PCR analysis of gene expression in different tissues of PGPR-inoculated <i>A. cruentus</i> plants
<p>The expression levels of a battery of genes involved in C and N metabolism and transport... more <p>The expression levels of a battery of genes involved in C and N metabolism and transport were measured in roots (<b>A</b>), and leaves (<b>B</b>), of <i>A. cruentus</i> plants inoculated with <i>Burkholderia caribensis</i> XV. The relative expression levels were determined by qPCR at 3, 5 and 7 weeks after seed inoculation, using the 2<sup>−ΔΔCt</sup> method, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088094#pone.0088094-Livak1" target="_blank">[94]</a>. The bars represent mean values ± SE. Dashed lines indicate upper and lower limits beyond which genes were considered to be up- and down-regulated, respectively. Experiments were performed thrice, and results from a representative experiment are shown.</p
Expression of betacyanin biosynthetic genes in response to salt stress
<p>Relative expression levels<sup>2</sup> were determined in leaves, stems and ... more <p>Relative expression levels<sup>2</sup> were determined in leaves, stems and roots of <i>A. hypochondriacus</i> plants, with contrasting pigmentation patterns, subjected to salt stress. Induced or repressed levels of expression (i.e. relative expression ≥1.5 or ≤0.5) are shown in bold text and italics, respectively.</p>1<p>The genotypes examined in this study were <i>Ah</i> cv. Nutrisol (<i>AhNut</i>; with predominantly betacyanic leaves), <i>Ah</i> India Red (<i>AhIR</i>; with predominantly betacyanic stems) and <i>Ah</i> India Green (<i>AhIG</i>; with all tissues acyanic).</p>2<p>The fold change in the expression of the target genes was calculated using the 2<sup>−ΔΔCt</sup> method according to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099012#pone.0099012-Livak1" target="_blank">[76]</a>.</p
Expression levels of betacyanin biosynthetic genes in <i>A. hypochondriacus</i> plants with contrasting pigmentation patterns
<p>Real-time quantitative polymerase chain reaction analysis of the basal expression of 5 b... more <p>Real-time quantitative polymerase chain reaction analysis of the basal expression of 5 betacyanin biosynthetic genes in <b>(A)</b> leaves, <b>(B)</b> stems and <b>(C)</b> roots of three genotypes of <i>A. hypochondriacus</i> plants differing in their pigmentation patterns, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099012#pone-0099012-g001" target="_blank">Figure 1</a>. The genes analyzed were the following: a UDP-glucose: cyclo-DOPA 5-O glucosyltransferase (<i>cD5-GT</i>), two 4, 5-DOPA-extradiol-dioxygenase genes (<i>DODA-1</i> and <i>DODA-2</i>), a UDP-glucose: betanidin 5-<i>O</i>-glucosyltransferase (<i>B5-GT</i>), and an ortholog of the red beet cytochrome P-450 <i>R</i> gene (<i>CYP76</i>). Transcript levels of these genes were normalized using <i>A. hypochondricus</i> actin and tubulin, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099012#pone.0099012-Yuan1" target="_blank">[77]</a>. Data are means ± SE (n = 6). Different letters over the bars represent statistically different values at <i>P</i>≤0.05 (Tukey Kramer test). FW = fresh weight.</p
Properties of the rhizobacteria used in this study
Systemin (Sys) is an octadecapeptide which, upon wounding, is released from the carboxy terminus ... more Systemin (Sys) is an octadecapeptide which, upon wounding, is released from the carboxy terminus of its precursor, prosystemin(ProSys) to promote plant defenses. Recent findings on the disordered structure of ProSysprompted us to investigate a putative biological role of the whole precursor deprived of Sys peptide. We produced transgenic tomato plants expressing a truncated ProSys gene in which the exon coding for Sys was removed and compared their defense response with that induced by the exogenous application of the recombinant deleted ProSys[ProSys(1-178)].By combining protein structure analyses, transcriptomic analysis, gene expression profiling and bioassays with different pests we demonstrate that the truncated ProSys, that does not induce the endogenous ProSys gene, promotes defense barriers in tomato plants through a hormone independent defense pathway, likely associated with the production of oligogalacturonides (OGs). Both transgenic and plants treated with the recombinant...
Journal of the Science of Food and Agriculture, 2019
BACKGROUND: In contrast to commercial Diospyros species, Mesoamerican fruit producing species are... more BACKGROUND: In contrast to commercial Diospyros species, Mesoamerican fruit producing species are scarcely known, in particular wild species that might harbor desirable traits suitable for breeding. Thus, metabolomic, chemical and antioxidant profiles of fruits harvested from cultivated Diospyros digyna and wild D. rekoi trees during consecutive winter seasons were obtained. Fruits were harvested in habitats having marked differences in soil quality, climate and luminosity. RESULTS: D. digyna fruits were larger and less acid than D. rekoi fruits, whereas antioxidant activity tended to be higher in D. rekoi fruits. Phenolic, flavonoid and sugar content also varied significantly between species. Metabolomic analysis allowed the pre-identification of 519 and 1665 metabolites in negative and positive electrospray ionization [ESI] modes, respectively. Principal component analysis of the positive ESI data explained 51.8% of the variance, and indicated clear metabolomic differences between D. rekoi and D. digyna fruits that was confirmed by DIESI-MS profiles. Twenty one discriminating metabolites were detected in fruits of both species; D. digyna fruits differentially accumulated lysophospholipids, whereas discriminating metabolites in D. rekoi fruits were chemically more diverse than those in D. digyna fruits. CONCLUSION: Domesticated D. digyna fruits have improved physicochemical fruit traits compared to wild D. rekoi fruits, including larger size and lower acidity. The metabolomic and chemical composition of their respective fruits was also significantly different, which in D. rekoi was manifested as a notable season-dependent increase in antioxidant capacity. Therefore, wild D.rekoi can be considered as an important genetic resource for the improvement of commercial Diospyros fruit quality.
Journal of Applied Microbiology, 2017
Article type : Original Article Translocation of Bacillus thuringiensis in Phaseolus vulgaris tis... more Article type : Original Article Translocation of Bacillus thuringiensis in Phaseolus vulgaris tissues and vertical transmission in Arabidopsis thaliana.
Background: Amaranthus hypochondriacus, a grain amaranth, is a C4 plant noted by its ability to t... more Background: Amaranthus hypochondriacus, a grain amaranth, is a C4 plant noted by its ability to tolerate stressful conditions and produce highly nutritious seeds. These possess an optimal amino acid balance and constitute a rich source of health-promoting peptides. Although several recent studies, mostly involving subtractive hybridization strategies, have contributed to increase the relatively low number of grain amaranth expressed sequence tags (ESTs), transcriptomic information of this species remains limited, particularly regarding tissue-specific and biotic stress-related genes. Thus, a large scale transcriptome analysis was performed to generate stem-and (a)biotic stress-responsive gene expression profiles in grain amaranth. Results: A total of 2,700,168 raw reads were obtained from six 454 pyrosequencing runs, which were assembled into 21,207 high quality sequences (20,408 isotigs + 799 contigs). The average sequence length was 1,064 bp and 930 bp for isotigs and contigs, respectively. Only 5,113 singletons were recovered after quality control. Contigs/ isotigs were further incorporated into 15,667 isogroups. All unique sequences were queried against the nr, TAIR, UniRef100, UniRef50 and Amaranthaceae EST databases for annotation. Functional GO annotation was performed with all contigs/isotigs that produced significant hits with the TAIR database. Only 8,260 sequences were found to be homologous when the transcriptomes of A. tuberculatus and A. hypochondriacus were compared, most of which were associated with basic housekeeping processes. Digital expression analysis identified 1,971 differentially expressed genes in response to at least one of four stress treatments tested. These included several multiple-stressinducible genes that could represent potential candidates for use in the engineering of stress-resistant plants. The transcriptomic data generated from pigmented stems shared similarity with findings reported in developing stems of Arabidopsis and black cottonwood (Populus trichocarpa). Conclusions: This study represents the first large-scale transcriptomic analysis of A. hypochondriacus, considered to be a highly nutritious and stress-tolerant crop. Numerous genes were found to be induced in response to (a)biotic stress, many of which could further the understanding of the mechanisms that contribute to multiple stressresistance in plants, a trait that has potential biotechnological applications in agriculture.
Physiologia Plantarum, 2009
A suppression‐subtractive‐hybridization (SSH) strategy was used to identify genes whose expressio... more A suppression‐subtractive‐hybridization (SSH) strategy was used to identify genes whose expression was modified in response to virus‐free whitefly Bemisia tabaci (Bt, biotype A) infestation in tomato (Solanum lycopersicum) plants. Thus, forward and reverse SSH gene libraries were generated at four points in the whitefly's life cycle, namely at (1) 2 days (adult feeding and oviposition: phase I); (2) 7 days (mobile crawler stage: phase II); (3) 12 days (second to third instar nymphal transition: phase III) and (4) 18 days (fourth instar nymphal stage: phase IV). The 169 genes with altered expression (up and downregulated) that were identified in the eight generated SSH libraries, together with 75 additional genes that were selected on the basis of their involvement in resistance responses against phytofagous insects and pathogens, were printed on a Nexterion® Slide MPX 16 to monitor their pattern of expression at the above phases. The results indicated that Bt infestation in toma...
BMC Plant Biology, 2012
Background Amaranthus cruentus and A. hypochondriacus are crop plants grown for grain production ... more Background Amaranthus cruentus and A. hypochondriacus are crop plants grown for grain production in subtropical countries. Recently, the generation of large-scale transcriptomic data opened the possibility to study representative genes of primary metabolism to gain a better understanding of the biochemical mechanisms underlying tolerance to defoliation in these species. A multi-level approach was followed involving gene expression analysis, enzyme activity and metabolite measurements. Results Defoliation by insect herbivory (HD) or mechanical damage (MD) led to a rapid and transient reduction of non-structural carbohydrates (NSC) in all tissues examined. This correlated with a short-term induction of foliar sucrolytic activity, differential gene expression of a vacuolar invertase and its inhibitor, and induction of a sucrose transporter gene. Leaf starch in defoliated plants correlated negatively with amylolytic activity and expression of a β-amylase-1 gene and positively with a sol...
Biologia plantarum, 2004
Amaranthus hypochondriacus plants were grown under three photosynthetic photon flux densities (PP... more Amaranthus hypochondriacus plants were grown under three photosynthetic photon flux densities (PPFD). Mature plants grown at full sunlight (38.8 mol m 2 d-') had higher maximum net photosynthetic rate (PN) and significantly higher leaf trypsin inhibitor activity than plants that developed under lower PPFD (19.4 and 12.8 mol m 2 d'). In contrast, seeds collected from plants fully exposed to sunlight showed the lowest activity of trypsin inhibitor, higher rate of germination and susceptibility to infection by Aspergillus niger.
At world level, Mexico is located as an important participant in fresh mango production and trade... more At world level, Mexico is located as an important participant in fresh mango production and trade, standing out as producer (2 * 10 6 t year-1) and exporter (2 * 10 5 t year-1) of mango and importer of minimum quantities with regard to its production and export. The economic activity around mango, is integrated by a group of stages that goes from fruit production to its consumption, which has been called value chain of mango; with base in scientific and empiric knowledge in relation to this cultivation, the critical points of more impact on this value chain in Mexico that we have detected, with negative repercussions for the economy of fruit farmers, are located in stages: production (flowering and anthracnosis) and industrialization (use of vegetable residuals generated in fresh mango process of industrialization). This revision presents a global analysis of stages that put in risk to this value chain in Mexico, as well as the causes and possible solutions to these, by means of alternatives that optimize its integral handling.
Rev Mex …, 2007
2007 Miriam Tejeda Sartorius / Norma Angélica Martínez Gallardo / Víctor Olalde Portugal / John P... more 2007 Miriam Tejeda Sartorius / Norma Angélica Martínez Gallardo / Víctor Olalde Portugal / John Paul Délano Frier JASMONIC ACID ACCELERATES THE EXPRESSION OF A PATHOGEN-SPECIFIC LIPOXYGENASE (POTLX-3) AND DELAYS FOLIAR LATE BLIGHT DEVELOPMENT IN ...
Critical Aspects on the Integral Management of Mango: Flowering, Anthracnosis and Industrial Waste
At world level, Mexico is located as an important participant in fresh mango production and trade... more At world level, Mexico is located as an important participant in fresh mango production and trade, standing out as producer (2∗106 t year-1) and exporter (2∗105 t year-1) of mango and importer of minimum quantities with regard to its production and export. The economic activity around mango, is integrated by a group of stages that goes from fruit production to its consumption, which has been called value chain of mango; with base in scientific and empiric knowledge in relation to this cultivation, the critical points of more impact on this value chain in Mexico that we have detected, with negative repercussions for the economy of fruit farmers, are located in stages: production (flowering and anthracnosis) and industrialization (use of vegetable residuals generated in fresh mango process of industrialization). This revision presents a global analysis of stages that put in risk to this value chain in Mexico, as well as the causes and possible solutions to these, by means of alternati...
Inoculation of tomato plants (Solanum lycopersicum) with growth-promoting Bacillus subtilis retards whitefly Bemisia tabaci development
Planta, 2009
Phytoparasitica, 2009
Mexico is considered to be one of the centers of origin of grain amaranth species. Recently, plan... more Mexico is considered to be one of the centers of origin of grain amaranth species. Recently, plants with abnormal anatomical features were observed in experimental fields established in Central Mexico. The most noticeable symptoms, which consisted of excessive stem and bud proliferation, mosaics and unusual coloration, suggested that they might be phytoplasma-induced disorders. Thus, different accessions of grain amaranth (Amaranthus hypochondriacus and A. cruentus) plants were subjected to polymerase chain reaction (PCR) analysis specifically designed to detect these pathogens. Two universal phytoplasma-specific primer pairs were tested in a nested PCR assay, with primer pair P1/tint (followed by primer pair R16F2/R16R2). Further DNA sequencing analysis of the resulting amplicons indicated that these phytoplasmas may be related to others already affecting important agricultural crops in Mexico, such as soybean. Data are presented that disclose the etiology of these syndromes by the use of molecular techniques. To the best of our knowledge, this finding constitutes the first report of a phytoplasma-related disease in grain amaranth.
Metabolic fingerprints for suboptimal mycorrhizal colonization in wild-type and the jasmonic acid deficient spr2 tomato mutant
Raw data for metabolic fingerprinting of tomato roots by DLI-ESI-MS to examine the effect of myco... more Raw data for metabolic fingerprinting of tomato roots by DLI-ESI-MS to examine the effect of mycorrhizal colonization on the global metabolic profile of WT and <em>spr2</em> mutant plants. Data were acquired in positive mode by direct liquid introduction electrospray ionization (DLI-MS).
Systemic Wound Signaling in Plants
Signaling and Communication in Plants, 2013
Ever since the seminal discovery of systemic wound signaling in tomato and potato plants by Green... more Ever since the seminal discovery of systemic wound signaling in tomato and potato plants by Green and Ryan (Science 1972), a number of candidate systemic wound signals have been proposed. These can be classified into three groups: (1) Chemical signals, including the alarm hormone systemin and other peptide hormones, jasmonic acid is a phytohormone, as well as reactive oxygen species (ROS); (2) physical signals, including electrical and hydraulic signals; and (3) herbivore-induced volatile compounds, including green leafy volatiles and terpenes. These signals are generated at or close to the site of herbivore-inflicted injury and systemically move to target tissues where they induce defense responses. Chemical and physical signals depend on the connectivity of the plant body, whereas volatile compounds are released into the airspace. Different plants with different morphologies and ecological niches employ different modes of systemic signaling.
Identification of genetic and biochemical mechanisms associated with heat shock and heat stress adaptation in grain amaranths
Frontiers in Plant Science
Heat stress is poised to become a major factor negatively affecting plant performance worldwide. ... more Heat stress is poised to become a major factor negatively affecting plant performance worldwide. In terms of world food security, increased ambient temperatures are poised to reduce yields in cereals and other economically important crops. Grain amaranths are known to be productive under poor and/or unfavorable growing conditions that significantly affect cereals and other crops. Several physiological and biochemical attributes have been recognized to contribute to this favorable property, including a high water-use efficiency and the activation of a carbon starvation response. This study reports the behavior of the three grain amaranth species to two different stress conditions: short-term exposure to heat shock (HS) conditions using young plants kept in a conditioned growth chamber or long-term cultivation under severe heat stress in greenhouse conditions. The latter involved exposing grain amaranth plants to daylight temperatures that hovered around 50°C, or above, for at least 4...
Plant Science, 2018
Jasmonic acid (JA) is a phytohormone involved in plant development and defense. A major role of J... more Jasmonic acid (JA) is a phytohormone involved in plant development and defense. A major role of JA is the enhancement of secondary metabolite production, such as response to herbivory. Systemin is a bioactive plant peptide of 18 amino acids that contributes to the induction of local and systemic defense responses in tomato (Solanum lycopersicum) through JA biosynthesis. The overexpression of systemin (PS-OE) results in constitutive JA accumulation and enhances pest resistance in plants. Conversely, mutant plants affected in linolenic acid synthesis (spr2) are negatively compromised in the production of JA which favors damage and oviposition by insect herbivores. With undirected mass fingerprinting analyses, we found global metabolic differences between genotypes with modified jasmonic acid production. The spr2 mutants were enriched in di-unsaturated fatty acids and generally showed more changes. The PS-OE genotype produced an unidentified compound with a mass-to-charge ratio of 695 (MZ695). Most strikingly, the steroidal glycoalkaloid biosynthesis was negatively affected in the spr2 genotype. Complementation with jasmonic acid could restore the tomatine pathway, which strongly suggests the control of steroidal glycoalkaloid biosynthesis by jasmonic acid. spr2 plants were more susceptible to fungal infection with Fusarium oxysporum f.sp. ciceris, but not to bacterial infection with Clavibacter michiganensis subsp. michiganensis which supports the involvement of steroidal glycoalkaloids in the plant response against fungi.
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Papers by John P Délano-Frier